Progress in Histochemistry and Cytochemistry最新文献

{"title":"Application of laser-capture microdissection to analysis of gene expression in the testis","authors":"Pavel Sluka ,&nbsp;Liza O’Donnell ,&nbsp;Robert I. McLachlan ,&nbsp;Peter G. Stanton","doi":"10.1016/j.proghi.2007.10.001","DOIUrl":"10.1016/j.proghi.2007.10.001","url":null,"abstract":"<div><p>The isolation and molecular analysis of highly purified cell populations from complex, heterogeneous tissues has been a challenge for many years. Spermatogenesis<span> in the testis<span> is a particularly difficult process to study given the unique multiple cellular associations within the seminiferous epithelium, making the isolation of specific cell types difficult. Laser-capture microdissection (LCM) is a recently developed technique that enables the isolation of individual cell populations from complex tissues. This technology has enhanced our ability to directly examine gene expression in enriched testicular cell populations by routine methods of gene expression analysis, such as real-time RT-PCR, differential display, and gene microarrays. The application of LCM has however introduced methodological hurdles that have not been encountered with more conventional molecular analyses of whole tissue. In particular, tissue handling (i.e. fixation, storage, and staining), consumables (e.g. slide choice), staining reagents (conventional H&amp;E vs. fluorescence), extraction methods, and downstream applications have all required re-optimisation to facilitate differential gene expression analysis using the small amounts of material obtained using LCM. This review will discuss three critical issues that are essential for successful procurement of cells from testicular tissue sections; tissue morphology, capture success, and maintenance of molecular integrity. The importance of these issues will be discussed with specific reference to the two most commonly used LCM systems; the Arcturus PixCell IIe and PALM systems. The rat testis will be used as a model, and emphasis will be placed on issues of tissue handling, processing, and staining methods, including the application of fluorescence techniques to assist in the identification of cells of interest for the purposes of mRNA expression analysis.</span></span></p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"42 4","pages":"Pages 173-201"},"PeriodicalIF":0.0,"publicationDate":"2008-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2007.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27238038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ifc Editorial Board","authors":"","doi":"10.1016/S0079-6336(08)00003-X","DOIUrl":"https://doi.org/10.1016/S0079-6336(08)00003-X","url":null,"abstract":"","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"42 4","pages":"Page IFC"},"PeriodicalIF":0.0,"publicationDate":"2008-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0079-6336(08)00003-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91720215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modern techniques for the diagnostic evaluation of the trephine bone marrow biopsy: Methodological aspects and applications","authors":"Falko Fend ,&nbsp;Alexandar Tzankov ,&nbsp;Karin Bink ,&nbsp;Stefan Seidl ,&nbsp;Leticia Quintanilla-Martinez ,&nbsp;Marcus Kremer ,&nbsp;Stephan Dirnhofer","doi":"10.1016/j.proghi.2007.10.002","DOIUrl":"10.1016/j.proghi.2007.10.002","url":null,"abstract":"<div><p><span>Histopathological examination of a bone marrow (BM) trephine biopsy is an integral part of the diagnostic work-up of patients with haematological disorders and other diseases which may afflict hematopoiesis. Until recently, the dramatic increase in modern immunological and molecular techniques which have been added to the diagnostic repertoire of clinical haematology has largely bypassed the BM trephine. In recent years, however, many of the technical obstacles preventing application of these techniques to BM biopsies have been surmounted, and immunohistochemistry, fluorescence </span><em>in situ</em><span><span> hybridization and polymerase chain reaction<span> (PCR)-based molecular techniques for examination of DNA and </span></span>RNA have successfully been applied to conventionally processed BM trephines. This review tries to give an overview of techniques suitable for trephine biopsies, as well as diagnostic and research applications.</span></p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"42 4","pages":"Pages 203-252"},"PeriodicalIF":0.0,"publicationDate":"2008-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2007.10.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27238042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ifc Editorial Board","authors":"","doi":"10.1016/S0079-6336(07)00033-2","DOIUrl":"https://doi.org/10.1016/S0079-6336(07)00033-2","url":null,"abstract":"","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"42 3","pages":"Page IFC"},"PeriodicalIF":0.0,"publicationDate":"2007-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0079-6336(07)00033-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136603358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of angiogenesis: Wound healing as a model","authors":"Sabine A. Eming ,&nbsp;Bent Brachvogel ,&nbsp;Teresa Odorisio ,&nbsp;Manuel Koch","doi":"10.1016/j.proghi.2007.06.001","DOIUrl":"10.1016/j.proghi.2007.06.001","url":null,"abstract":"<div><p><span>Normal tissue function requires adequate supply of oxygen through blood vessels. Understanding how blood vessels form is a challenging objective because angiogenesis is vital to many physiological and pathological processes. Unraveling mechanisms of angiogenesis would offer therapeutic options to ameliorate disorders that are currently leading causes of mortality and morbidity, including cardiovascular diseases, cancer, chronic </span>inflammatory disorders<span><span><span>, diabetic retinopathy, excessive tissue defects, and chronic non-healing wounds. Restoring blood flow to the site of injured tissue is a prerequisite for mounting a successful repair response, and wound angiogenesis represents a paradigmatic model to study molecular mechanisms involved in the formation and remodeling of vascular structures. In particular, repair of skin defects offers an ideal model to analyze angiogenesis due to its easy accessibility to control and manipulate this process. Most of those growth factors, extracellular matrix molecules, and cell types, recently discovered and considered as crucial factors in blood vessel formation, have been identified and analyzed during skin repair and the process of wound angiogenesis. This article will review cellular and molecular mechanisms controlling angiogenesis in cutaneous tissue repair in light of recent reports and data from our laboratories. In this article we will discuss the contribution of growth factors, </span>basement membrane molecules, and </span>mural cells in wound angiogenesis. The article provides a rationale for targeting the angiogenic response in order to modulate the outcome of the healing response.</span></p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"42 3","pages":"Pages 115-170"},"PeriodicalIF":0.0,"publicationDate":"2007-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2007.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41012598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ifc Editorial Board","authors":"","doi":"10.1016/S0079-6336(07)00022-8","DOIUrl":"https://doi.org/10.1016/S0079-6336(07)00022-8","url":null,"abstract":"","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"42 2","pages":"Page IFC"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0079-6336(07)00022-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136983850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression profiles of MUC mucins and trefoil factor family (TFF) peptides in the intrahepatic biliary system: Physiological distribution and pathological significance","authors":"Motoko Sasaki,&nbsp;Hiroko Ikeda,&nbsp;Yasuni Nakanuma","doi":"10.1016/j.proghi.2007.02.001","DOIUrl":"10.1016/j.proghi.2007.02.001","url":null,"abstract":"<div><p>Mucin secreted by mucosal epithelial cells plays a role in the protection of the mucosal surface and also is involved in pathological processes. So far, MUC1–4, 5AC, 5B, 6–8, 11–13 and 15–17 genes coding the backbone mucin core protein have been identified in humans. Their diverse physiological distribution and pathological alterations have been reported. Trefoil factor family (TFF) peptides are mucin-associated molecules co-expressed with MUC mucins and involved in the maintenance of mucosal barrier and the biological behavior of epithelial and carcinoma cells<span><span>. Intrahepatic biliary system is a route linking the bile canaliculi and the </span>extrahepatic bile duct<span> for the excretion of bile synthesized by hepatocytes. Biliary epithelial cells line in the intrahepatic biliary system, secreting mucin and other molecules involved in the maintenance and regulation of the system. In this review, the latest information regarding properties, expression profiles and regulation of MUC mucins and TFF peptides in the intrahepatic biliary system is summarized. In particular, we focus on the expression profiles and their significance of MUC mucins in developmental and normal livers, various hepatobiliary diseases and intrahepatic cholangiocarcinoma.</span></span></p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"42 2","pages":"Pages 61-110"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2007.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26816224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of tight junctions in cell proliferation and cancer","authors":"Lorenza González-Mariscal,&nbsp;Susana Lechuga ,&nbsp;Erika Garay","doi":"10.1016/j.proghi.2007.01.001","DOIUrl":"10.1016/j.proghi.2007.01.001","url":null,"abstract":"<div><p><span>The acquisition of a cancerous phenotype by epithelial cells involves the disruption of intercellular adhesions. The reorganization of the E-cadherin/</span><em>β</em><span>-catenin complex in adherens junctions<span> during cell transformation is widely recognized. Instead the implication of tight junctions (TJs) in this process is starting to be unraveled. The aim of this article is to review the role of TJ proteins in cell proliferation and cancer.</span></span></p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"42 1","pages":"Pages 1-57"},"PeriodicalIF":0.0,"publicationDate":"2007-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2007.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26722741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ifc Editorial Board","authors":"","doi":"10.1016/S0079-6336(07)00011-3","DOIUrl":"https://doi.org/10.1016/S0079-6336(07)00011-3","url":null,"abstract":"","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"42 1","pages":"Page IFC"},"PeriodicalIF":0.0,"publicationDate":"2007-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0079-6336(07)00011-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137217925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatocellular expression of glutamine synthetase: An indicator of morphogen actions as master regulators of zonation in adult liver","authors":"Rolf Gebhardt,&nbsp;Alicja Baldysiak-Figiel,&nbsp;Vera Krügel,&nbsp;Elke Ueberham,&nbsp;Frank Gaunitz","doi":"10.1016/j.proghi.2006.12.001","DOIUrl":"10.1016/j.proghi.2006.12.001","url":null,"abstract":"<div><p><span><span>Glutamine synthetase<span> (GS) has long been known to be expressed exclusively in pericentral hepatocytes most proximal to the central veins<span> of liver lobuli. This enzyme as well as its peculiar distribution complementary to the periportal compartment for </span></span></span>ureogenesis<span><span> plays an important role in nitrogen metabolism, particularly in </span>homeostasis of blood levels of ammonium ions and glutamine. Despite this fact and intensive studies </span></span><em>in vivo</em> and <em>in vitro</em><span><span>, many aspects of the regulation of its activity on the protein and on the genetic level remained enigmatic. Recent experimental advances using </span>transgenic mice<span><span> and new analytic tools have revealed the fundamental role of morphogens such as wingless-type </span>MMTV integration site family member signals (Wnt), </span></span><em>β</em><span><span><span>-catenin, and adenomatous polyposis coli in the regulation of this particular enzyme. In addition, novel information concerning the structure of transcription factor binding sites within regulatory regions of the GS gene and their interactions with </span>signalling pathways could be collected. In this review we focus on all aspects of the regulation of GS in the liver and demonstrate how the new findings have changed our view of the determinants of liver zonation. What appeared as a simple response of hepatocytes to blood-derived factors and local </span>cellular interactions<span> must now be perceived as a fundamental mechanism of adult tissue patterning by morphogens that were considered mainly as regulators of developmental processes. Though GS may be the most obvious indicator of morphogen action among many other targets, elucidation of the complex regulation of the expression of the GS gene could pave the road for a better understanding of the mechanisms involved in patterning of liver parenchyma. Based on current knowledge we propose a new concept of how morphogens, hormones and other factors may act in concert, in order to restrict gene expression to small subpopulations of one differentiated cell type, the hepatocyte, in different anatomical locations. Although many details of this regulatory network are still missing, and an era of exciting new discoveries is still about to come, it can already be envisioned that similar mechanisms may well be active in other organs contributing to the fine-tuning of organ-specific functions.</span></span></p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"41 4","pages":"Pages 201-266"},"PeriodicalIF":0.0,"publicationDate":"2007-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2006.12.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26610619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}