Progress in Histochemistry and Cytochemistry最新文献_第8页

Progress in Histochemistry and Cytochemistry Pub Date : 2007-04-17 DOI: 10.1016/S0079-6336(07)00005-8

引用次数: 0

Heat-induced antigen retrieval: Mechanisms and application to histochemistry 热诱导抗原回收:机制及其在组织化学中的应用

Progress in Histochemistry and Cytochemistry Pub Date : 2007-01-25 DOI: 10.1016/j.proghi.2006.09.001

Shuji Yamashita

{"title":"Heat-induced antigen retrieval: Mechanisms and application to histochemistry","authors":"Shuji Yamashita","doi":"10.1016/j.proghi.2006.09.001","DOIUrl":"10.1016/j.proghi.2006.09.001","url":null,"abstract":"<div><p><span>Since the introduction of the fluorescence-labeled antibody method by Coons et al. [Immunological properties of antibody containing a fluorescent group. Proc Soc Exp Biol Med 47, 200–2002], many immunohistochemical methods have been refined to obtain high sensitivity with low background staining at both light and electron microscopic levels. Heat-induced antigen retrieval (HIAR) reported by Shi et al. in the early 1990s has greatly contributed to immunohistochemical analysis for formalin-fixed and paraffin-embedded (FFPE) materials, particularly in the field of pathology. Although antigen retrieval techniques including enzyme digestion, treatment with protein denaturants and heating have been considered tricky and mysterious techniques, the mechanisms of HIAR have been rapidly elucidated. Heating cleaves crosslinks (methylene bridges) and add methylol groups in formaldehyde-fixed proteins and </span>nucleic acids<span> and extends polypeptides to unmask epitopes hidden in the inner portion of antigens or covered by adjacent macromolecules. In buffers having an appropriate pH and ion concentration, epitopes are exposed without entangling the extended polypeptides during cooling process, since polypeptides may strike a balance between hydrophobic attraction force and electrostatic repulsion force.</span></p><p><span>Recent studies have demonstrated that HIAR is applicable for immunohistochemistry with various kinds of specimens, i.e., FFPE materials, frozen sections, plastic-embedded specimens, and physically fixed tissues at both the light- and electron-microscopic levels, and have suggested that the mechanism of HIAR is common to aldehyde-fixed and aldehyde-unfixed materials. Furthermore, heating has been shown to be effective for flow cytometry, nucleic acid histochemistry (fluorescein </span>in situ hybridization (FISH), in situ hybridization (ISH), and terminal deoxynucleotidyl transferase-mediated nick labeling (TUNEL)), and extraction and analysis of macromolecules in both FFPE archive materials and specimens processed by other procedures. In this article, we review mechanism of HIAR and application of heating in both immunohistochemistry and other histochemical reactions.</p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"41 3","pages":"Pages 141-200"},"PeriodicalIF":0.0,"publicationDate":"2007-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2006.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26467751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 166

ifc Editorial Board ifc编辑委员会

Progress in Histochemistry and Cytochemistry Pub Date : 2007-01-25 DOI: 10.1016/S0079-6336(06)00063-5

引用次数: 0

ifc Editorial Board ifc编辑委员会

Progress in Histochemistry and Cytochemistry Pub Date : 2006-09-21 DOI: 10.1016/S0079-6336(06)00054-4

引用次数: 0

Silver enhancement of quantum dots resulting from (1) metabolism of toxic metals in animals and humans, (2) in vivo, in vitro and immersion created zinc–sulphur/zinc–selenium nanocrystals, (3) metal ions liberated from metal implants and particles 由于(1)动物和人体内有毒金属的代谢，(2)体内、体外和浸泡产生锌硫/锌硒纳米晶体，(3)从金属植入物和颗粒中释放的金属离子，导致量子点的银增强

Progress in Histochemistry and Cytochemistry Pub Date : 2006-09-21 DOI: 10.1016/j.proghi.2006.06.001

Gorm Danscher, Meredin Stoltenberg

{"title":"Silver enhancement of quantum dots resulting from (1) metabolism of toxic metals in animals and humans, (2) in vivo, in vitro and immersion created zinc–sulphur/zinc–selenium nanocrystals, (3) metal ions liberated from metal implants and particles","authors":"Gorm Danscher, Meredin Stoltenberg","doi":"10.1016/j.proghi.2006.06.001","DOIUrl":"10.1016/j.proghi.2006.06.001","url":null,"abstract":"<div><p>Autometallographic (AMG) silver enhancement is a potent histochemical tool for tracing a variety of metal containing nanocrystals, e.g. pure gold and silver nanoclusters and quantum dots of silver, mercury, bismuth or zinc, with sulphur and/or selenium.</p><p>These nanocrystals can be created in many different ways, e.g. (1) by manufacturing colloidal gold or silver particles, (2) by treating an organism in vivo with sulphide or selenide<span><span> ions, (3) as the result of a metabolic decomposition of bismuth-, mercury- or silver-containing macromolecules in </span>cell organelles, or (4) as the end product of histochemical processing of tissue sections. Such nano-sized AMG nanocrystals can then be silver-amplified several times of magnitude by being exposed to an AMG developer, i.e. a normal photographic developer enriched with silver ions.</span></p><p><span><span>The present monograph attempts to provide a review of the autometallographic silver amplification techniques known today and their use in biology. After achieving a stronghold in histochemistry by Timm's introduction of the “silver-sulphide staining” in 1958, the AMG technique has evolved and expanded into several different areas of research, including </span>immunocytochemistry, tracing of enzymes at LM and EM levels, blot staining, retrograde axonal tracing of zinc-enriched (ZEN) neurons, counterstaining of semithin sections, enhancement of histochemical reaction products, marking of phagocytotic cells, staining of </span>myelin, tracing of gold ions released from gold implants, and visualization of capillaries.</p><p>General technical comments, protocols for the current AMG methods and a summary of the most significant scientific results obtained by this wide variety of AMG histochemical approaches are included in the present article.</p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"41 2","pages":"Pages 57-139"},"PeriodicalIF":0.0,"publicationDate":"2006-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2006.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26233961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 107

Mucins and TFF peptides of the tear film and lacrimal apparatus 泪膜和泪器的粘蛋白和TFF肽

Progress in Histochemistry and Cytochemistry Pub Date : 2006-07-17 DOI: 10.1016/j.proghi.2006.03.001

Friedrich P. Paulsen , Monica S. Berry

引用次数: 95

ifc Editorial Board ifc编辑委员会

Progress in Histochemistry and Cytochemistry Pub Date : 2006-07-17 DOI: 10.1016/S0079-6336(06)00042-8

引用次数: 0

Hypercytolipidemia-induced cellular lipoapoptosis: Cytostructural and endometabolic basis of progressive organo-involution following expression of diabetes (db/db) and obese (ob/ob) mutation syndromes 高脂血症诱导的细胞脂肪凋亡:糖尿病(db/db)和肥胖(ob/ob)突变综合征表达后进行性器官退化的细胞结构和内膜代谢基础

Progress in Histochemistry and Cytochemistry Pub Date : 2006-07-05 DOI: 10.1016/j.proghi.2006.02.002

David R. Garris

{"title":"Hypercytolipidemia-induced cellular lipoapoptosis: Cytostructural and endometabolic basis of progressive organo-involution following expression of diabetes (db/db) and obese (ob/ob) mutation syndromes","authors":"David R. Garris","doi":"10.1016/j.proghi.2006.02.002","DOIUrl":"10.1016/j.proghi.2006.02.002","url":null,"abstract":"<div><p>Onset expression of Type 2 (NIDDM) diabetes and obesity metabolic syndromes (DOS) are characterized by premature, progressive cytoatrophy and organo-involution induced by dysregulated cellular gluco- and lipo-metabolic cascades. The consequential systemic, interstitial and intracellular hyperlipidemia<span><span> disrupts normal cytointegrity and metabolic responsivity to the established hypercaloric pericellular environments. The sequential cytostructural, metabolic and endocrine disturbances associated with the development of progressive DOS-associated hypercytolipidemia compromises cellular metabolic response cascades and promotes cytochemical disturbances which culminate with nuclear lipoapoptosis and cytoatrophy. The dramatic alterations in interstitial glucose and lipid (free fatty acids/triglycerides) concentrations are recognized to influence interstitial and cytoplasmic microchemical environments, which markedly alter cellular nutrient diffusion and active trans-membrane flux rates. The progressive exacerbation of interstitial and cytoplasmic lipid imbibition has been demonstrated to be associated with </span>DNA fragmentation by lipo-infiltration into the chromatin matrix, inducing structural disruption and physical dissolution, indexed as nuclear lipoapoptosis. Therapeutic reduction of the severity of hypercytolipidemia-induced structural and cytochemical compromise promotes the restoration of homeostatic metabolic support for normalized cytostructural indices and supportive cellular gluco- and lipo-metabolic cascades. The re-establishment of a homeostatic interstitial microenvironment moderates the severity of cytolipidemic compromise within affected cell types, reduces nuclear lipo-infiltration and DNA lipo-dissolution, resulting in the preservation of cytostructural integrity. Through the therapeutic restoration of extra- and intra-cellular microchemical environments in genetically dysregulated metabolic syndrome models, the coincident cytochemical, endocrine and metabolic disturbances associated with progressive hypercytolipidemia, resulting from the expressed systemic hypercaloric environmental and hepato-pancreatic endometabolic disturbances which characterize Type 2 (NIDDM) diabetes–obesity and metabolic (X) syndromes, may be ameliorated.</span></p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"40 4","pages":"Pages 181-231"},"PeriodicalIF":0.0,"publicationDate":"2006-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2006.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26080740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

ifc Editorial Board ifc编辑委员会

Progress in Histochemistry and Cytochemistry Pub Date : 2006-07-05 DOI: 10.1016/S0079-6336(06)00025-8

引用次数: 0

The anatomy of organogenesis: Novel solutions to old problems 器官发生解剖学:老问题的新解决方案

Progress in Histochemistry and Cytochemistry Pub Date : 2006-07-03 DOI: 10.1016/j.proghi.2006.02.001

Jamie A. Davies, Jane E. Armstrong

{"title":"The anatomy of organogenesis: Novel solutions to old problems","authors":"Jamie A. Davies, Jane E. Armstrong","doi":"10.1016/j.proghi.2006.02.001","DOIUrl":"10.1016/j.proghi.2006.02.001","url":null,"abstract":"<div><p><span>Understanding anatomical aspects of mammalian organ development, in both normal and mutant animals, is important for basic biology and also for regenerative medicine and tissue engineering. The size and complexity of developing organs, together with variations in their detailed anatomy, has made the obtaining of high-resolution time-courses of anatomical change difficult to obtain. The fact that organ development tends to use the same genes as early </span>embryogenesis also makes genetic manipulation difficult, as so many mutant embryos die before organogenesis begins. These problems have seriously hampered the study of organogenesis. Here, we describe three significant advances that promise solutions: (1) the production of GFP-reporter mice that can be used for high-resolution live-imaging of small tissues as they grow, (2) RNA interference, which allows the manipulation of specific genes at any stage of organ development, and (3) optical projection tomography, which allows medium-resolution three-dimensional images of complete embryos to be obtained easily. We finish by looking ahead to the prospects of uniting these three technologies to allow accurate, high-throughput screening of mutants and automated comparison of biological data with computer predictions.</p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"40 3","pages":"Pages 165-176"},"PeriodicalIF":0.0,"publicationDate":"2006-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2006.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26075598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9