Progress in Histochemistry and Cytochemistry最新文献

Intracellular and extracellular microRNA: An update on localization and biological role 细胞内和细胞外microRNA:定位和生物学作用的最新进展

Progress in Histochemistry and Cytochemistry Pub Date : 2016-11-01 DOI: 10.1016/j.proghi.2016.06.001

Julia A. Makarova , Maxim U. Shkurnikov , Daniel Wicklein , Tobias Lange , Timur R. Samatov , Andrey A. Turchinovich , Alexander G. Tonevitsky

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引用次数: 178

Protein multiplicity can lead to misconduct in western blotting and misinterpretation of immunohistochemical staining results, creating much conflicting data 蛋白质多样性可能导致免疫印迹的不当行为和免疫组织化学染色结果的误解，产生许多相互矛盾的数据

Progress in Histochemistry and Cytochemistry Pub Date : 2016-11-01 DOI: 10.1016/j.proghi.2016.11.001

Xingde Liu , Yiming Wang , Wenxiu Yang , Zhizhong Guan , Wenfeng Yu , D.Joshua Liao

{"title":"Protein multiplicity can lead to misconduct in western blotting and misinterpretation of immunohistochemical staining results, creating much conflicting data","authors":"Xingde Liu , Yiming Wang , Wenxiu Yang , Zhizhong Guan , Wenfeng Yu , D.Joshua Liao","doi":"10.1016/j.proghi.2016.11.001","DOIUrl":"10.1016/j.proghi.2016.11.001","url":null,"abstract":"<div><p>Western blotting (WB) and immunohistochemical staining (IHC) are common techniques for determining tissue protein expression. Both techniques require a primary antibody specific for the protein in question. WB data is band(s) on a membrane while IHC result is a staining on a tissue section. Most human genes are known to produce multiple protein isoforms; in agreement with that, multiple bands are often found on the WB membrane. However, a common but unspoken practice in WB is to cut away the extra band(s) and present for publication only the band of interest, which implies to the readers that only one form of protein is expressed and thus the data interpretation is straightforward. Similarly, few IHC studies discuss whether the antibody used is isoform-specific and whether the positive staining is derived from only one isoform. Currently, there is no reliable technique to determine the isoform-specificity of an antibody, especially for IHC. Therefore, cutting away extra band(s) on the membrane usually is a form of misconduct in WB, and a positive staining in IHC only indicates the presence of protein product(s) of the to-be-interrogated gene, and not necessarily the presence of the isoform of interest. We suggest that data of WB and IHC involving only one antibody should not be published and that relevant reports should discuss whether there may be protein multiplicity and whether the antibody used is isoform-specific. Hopefully, techniques will soon emerge that allow determination of not only the presence of protein products of genes but also the isoforms expressed.</p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"51 3","pages":"Pages 51-58"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2016.11.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55032935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

ifc Editorial Board ifc编辑委员会

Progress in Histochemistry and Cytochemistry Pub Date : 2016-11-01 DOI: 10.1016/S0079-6336(16)30056-0

引用次数: 0

A survey of clearing techniques for 3D imaging of tissues with special reference to connective tissue 组织三维成像清除技术综述，特别是结缔组织

Progress in Histochemistry and Cytochemistry Pub Date : 2016-08-01 DOI: 10.1016/j.proghi.2016.04.001

Adriano Azaripour , Tonny Lagerweij , Christina Scharfbillig , Anna Elisabeth Jadczak , Brita Willershausen , Cornelis J.F. Van Noorden

{"title":"A survey of clearing techniques for 3D imaging of tissues with special reference to connective tissue","authors":"Adriano Azaripour , Tonny Lagerweij , Christina Scharfbillig , Anna Elisabeth Jadczak , Brita Willershausen , Cornelis J.F. Van Noorden","doi":"10.1016/j.proghi.2016.04.001","DOIUrl":"10.1016/j.proghi.2016.04.001","url":null,"abstract":"<div><p>For 3-dimensional (3D) imaging of a tissue, 3 methodological steps are essential and their successful application depends on specific characteristics of the type of tissue. The steps are 1° clearing of the opaque tissue to render it transparent for microscopy, 2° fluorescence labeling of the tissues and 3° 3D imaging. In the past decades, new methodologies were introduced for the clearing steps with their specific advantages and disadvantages. Most clearing techniques have been applied to the central nervous system and other organs that contain relatively low amounts of connective tissue including extracellular matrix. However, tissues that contain large amounts of extracellular matrix such as dermis in skin or gingiva are difficult to clear. The present survey lists methodologies that are available for clearing of tissues for 3D imaging. We report here that the BABB method using a mixture of benzyl alcohol and benzyl benzoate and iDISCO using dibenzylether (DBE) are the most successful methods for clearing connective tissue-rich gingiva and dermis of skin for 3D histochemistry and imaging of fluorescence using light-sheet microscopy.</p></div>","PeriodicalId":54550,"journal":{"name":"Progress in Histochemistry and Cytochemistry","volume":"51 2","pages":"Pages 9-23"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.proghi.2016.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34452781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 138

L1CAM: Cell adhesion and more l1:细胞粘附和更多

Progress in Histochemistry and Cytochemistry Pub Date : 2016-08-01 DOI: 10.1016/j.proghi.2016.05.001

Timur R. Samatov , Daniel Wicklein , Alexander G. Tonevitsky

引用次数: 63

ifc Editorial Board ifc编辑委员会

Progress in Histochemistry and Cytochemistry Pub Date : 2016-08-01 DOI: 10.1016/S0079-6336(16)30043-2

引用次数: 0

Mutated tau, amyloid and neuroinflammation in Alzheimer disease—A brief review 阿尔茨海默病中的突变tau蛋白、淀粉样蛋白和神经炎症

Progress in Histochemistry and Cytochemistry Pub Date : 2016-05-01 DOI: 10.1016/j.proghi.2016.01.001

A.S.M. Hung , Y. Liang , Tony C.H. Chow , H.C. Tang , Sharon L.Y. Wu , M.S.M. Wai , D.T. Yew

引用次数: 38

ifc Editorial Board ifc编辑委员会

Progress in Histochemistry and Cytochemistry Pub Date : 2016-05-01 DOI: 10.1016/S0079-6336(16)30009-2

引用次数: 0

ifc Editorial Board ifc编辑委员会

Progress in Histochemistry and Cytochemistry Pub Date : 2016-01-01 DOI: 10.1016/S0079-6336(16)00011-5

引用次数: 0

In situ hybridisation: Technologies and their application to understanding disease 原位杂交:技术及其在疾病认识中的应用

Progress in Histochemistry and Cytochemistry Pub Date : 2016-01-01 DOI: 10.1016/j.proghi.2015.12.001

Anthony Warford

引用次数: 15