Revista Do Instituto De Medicina Tropical De Sao Paulo最新文献

{"title":"Can archived lesion smears be used to identify Leishmania species in regions with high species diversity?","authors":"João Guilherme Pontes Lima Assy, Nara Karyne Delduck Feitosa, Jaqueline Alves Delprete, Vanessa Kehdy, Rose Grace Brito Marques, Expedito José de Albuquerque Luna, José Angelo Lauletta Lindoso, Lucia Maria Almeida Braz","doi":"10.1590/S1678-9946202567039","DOIUrl":"10.1590/S1678-9946202567039","url":null,"abstract":"<p><p>The identification of Leishmania species is crucial for eco-epidemiological purposes and may be useful for clinical management. Notably, archived smear slides can be valuable in this scenario. ITS-1 PCR followed by sequencing was used to identify Leishmania species from archived lesion smears of patients with suspected cutaneous leishmaniasis in Santarem city, Para State, Brazil. A total of 44 microscopically positive lesion smears were analyzed, of which 34 yielded positive PCR results. Of these, 22 were subjected to Sanger sequencing and 15 were successfully sequenced, revealing five Leishmania species. This study demonstrates the applicability of molecular testing on archived samples. ITS-1 sequencing effectively differentiated between species, revealing significant diversity of Leishmania in the Brazilian Amazon.</p>","PeriodicalId":54466,"journal":{"name":"Revista Do Instituto De Medicina Tropical De Sao Paulo","volume":"67 ","pages":"e39"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12204206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nipah virus infection: preparedness for the pathological diagnosis of an emerging Paramyxoviridae disease with epidemic potential.","authors":"Cinthya Dos Santos Cirqueira Borges, Ana Maria Gonçalves, Fernanda Alencar Rodrigues, Thais de Souza Lima, Ana Catharina Seixas Nastri, Venâncio Avancini Ferreira Alves, Mirian Nacagami Sotto, Thais Mauad, Soon Hao Tan, Paulo Hilário Nascimento Saldiva, Luiz Fernando Ferraz Silva, Kum Thong Wong, Marisa Dolhnikoff, Amaro Nunes Duarte-Neto","doi":"10.1590/S1678-9946202567040","DOIUrl":"10.1590/S1678-9946202567040","url":null,"abstract":"","PeriodicalId":54466,"journal":{"name":"Revista Do Instituto De Medicina Tropical De Sao Paulo","volume":"67 ","pages":"e40"},"PeriodicalIF":1.5,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12204208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastrointestinal mucormycosis associated with leptospirosis: should we be concerned during major floods?","authors":"Mariane Taborda, Juliana Possatto Fernandes Takahashi, Jessica de Brito Ferreira Nascimento, Julia Ferreira Mari, Vítor Falcão de Oliveira, Adriana Satie Gonçalves Kono Magri, Ana Catharina de Seixas Santos Nastri, Marcello Mihailenko Chaves Magri","doi":"10.1590/S1678-9946202567035","DOIUrl":"10.1590/S1678-9946202567035","url":null,"abstract":"<p><p>Gastrointestinal mucormycosis (GIM) and leptospirosis are two severe diseases associated with high morbidity and mortality rates. The coexistence of these two conditions has not yet been reported in the literature. This study presents a case involving this rare association. A 49-year-old man from Sao Paulo, Brazil, was hospitalized with acute abdominal pain, low blood pressure, and jaundice. He had a history of contact with floodwater and ingestion of contaminated water, was hospitalized with acute abdominal pain, low blood pressure, and jaundice. Upon ICU admission, he developed jaundice, acute renal failure requiring hemodialysis, and alveolar hemorrhage necessitating intubation. Leptospirosis was confirmed by serological tests and treated with ceftriaxone for 14 days. Two weeks later, he developed severe enterorrhagia, requiring a massive transfusion and a total colectomy with terminal ileostomy. Histopathology revealed necrotizing granulomatous inflammation with hyphae indicative of mucormycosis. He was treated with amphotericin B for 7 weeks, followed by posaconazole. Abdominal CT scans over the next five months showed complete clinical and radiological improvement. The association between mucormycosis and leptospirosis has not been previously documented, highlighting the diagnostic challenges and the critical importance of early detection. Successful management in this case required timely surgical intervention combined with prolonged antifungal therapy.</p>","PeriodicalId":54466,"journal":{"name":"Revista Do Instituto De Medicina Tropical De Sao Paulo","volume":"67 ","pages":"e35"},"PeriodicalIF":1.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The lingering crisis: gaps in long-term care for children with congenital Zika syndrome and their families in Brazil.","authors":"Paulo Ricardo Martins-Filho","doi":"10.1590/S1678-9946202567036","DOIUrl":"10.1590/S1678-9946202567036","url":null,"abstract":"","PeriodicalId":54466,"journal":{"name":"Revista Do Instituto De Medicina Tropical De Sao Paulo","volume":"67 ","pages":"e36"},"PeriodicalIF":1.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlamydia trachomatis infection and risk of ovarian cancer: a systematic review and meta-analysis.","authors":"Pei Wang, Xiuxiu You, Xianjing Zeng, Qingmei Peng","doi":"10.1590/S1678-9946202567034","DOIUrl":"10.1590/S1678-9946202567034","url":null,"abstract":"<p><p>Chlamydia trachomatis infection has been implicated as a potential risk factor for ovarian cancer (OC), but evidence remains inconclusive. This study aims to assess the association between C. trachomatis infection and OC risk using a systematic review and meta-analysis. A comprehensive literature search was conducted in PubMed, Embase, Scopus, Web of Science, and SciELO from their inception to October 2024. Observational studies examining the association between C. trachomatis infection and OC risk were included. Pooled odds ratios (ORs) were calculated using random-effects models. Subgroup and sensitivity analyses were performed based on diagnostic methods and control group types, and publication bias was assessed using Egger's test. Out of 3,288 records, 11 studies involving 4,518 participants were included. The overall meta-analysis revealed a non-significant association between C. trachomatis infection and OC risk (OR = 1.30, 95% CI = 0.99-1.70). However, sensitivity analysis excluding two studies demonstrated a significant association (OR = 1.37, 95% CI = 1.16-1.61). Subgroup analysis showed that PCR-diagnosed C. trachomatis infection was significantly associated with an increased risk (OR = 6.64, 95% CI = 1.62-25.71), while serology-based studies yielded non-significant results. Heterogeneity was high (I² = 70.83%), and publication bias was detected (Egger's test p = 0.015). These findings highlight the role of infection chronicity in OC pathogenesis and suggest that diagnostic methodology significantly impacts observed associations. Future research should employ longitudinal designs with serial molecular testing to establish temporality and evaluate whether targeted Chlamydia screening or treatment interventions could mitigate OC risk in high-prevalence populations.</p>","PeriodicalId":54466,"journal":{"name":"Revista Do Instituto De Medicina Tropical De Sao Paulo","volume":"67 ","pages":"e34"},"PeriodicalIF":1.5,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of SARS-CoV-2 reinfection among blood donors from two Brazilian states in the post-vaccination period: a prospective cohort study.","authors":"Monike Aparecida Matos de Oliveira, Tiane Sena de Castro, Renata Buccheri, Tassila Salomon, Carla Luana Dinardo, Isabel Cristina Gomes Moura, Myuki Alfaia Esashika Crispim, Nelson Abrahim Fraiji, Ester Cerdeira Sabino, Cecília Salete Alencar","doi":"10.1590/S1678-9946202567033","DOIUrl":"10.1590/S1678-9946202567033","url":null,"abstract":"<p><p>To assess SARS-CoV-2 reinfection incidence in the post-vaccination period, we carried out a prospective cohort study of blood donors from Amazonas and Sao Paulo States, Brazil. Anti-nucleocapsid immunoglobulin (IgG anti-N) tests carried out by blood centers in 2020 were used to identify previous SARS-CoV-2 infections in blood donors and divide them into two groups: prior infection (n=386) and no prior infection (n=111). From March 2021 to January 2022, donors were followed up for six months, during which IgG anti-N and real-time reverse transcription polymerase chain reaction tests were performed every two months to detect SARS-CoV-2 infections. Symptoms and vaccination status were also recorded. Most participants (93.6%) received at least one COVID-19 vaccine dose. Reinfection incidence in the prior infection group equaled 1.39 per 100 person-months (95% CI: 0.90-2.06), in comparison to 2.68 per 100 person-months (95% CI: 1.28-4.93) for new infections in those without prior infection. The incidence risk ratio showed no significant association (0.52, 95% CI: 0.25-1.13). However, prior infection significantly increased the probability of remaining uninfected (Log-rank: p=0.009). Most reinfections (84%) showed no symptoms and occurred post-vaccination during the Delta and Omicron waves. IgG anti-N seroprevalence decreased in the prior infection group (from 35.5% at baseline to 22.5% after six months, p=0.003). Despite no significant incidence risk ratio differences, donors with prior infection had lower infection rates and a higher likelihood of remaining uninfected. Persistent post-vaccination asymptomatic infections emphasize the need for ongoing prevention, genomic surveillance, and booster programs to address emerging variants and protect vulnerable populations.</p>","PeriodicalId":54466,"journal":{"name":"Revista Do Instituto De Medicina Tropical De Sao Paulo","volume":"67 ","pages":"e33"},"PeriodicalIF":1.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycobacterium marinum infection in an older woman with extensive verrucous skin lesions: a case report.","authors":"Hongyuan Shao, Yongxia Liu, Donghong Du, Hongqing Tian","doi":"10.1590/S1678-9946202567031","DOIUrl":"10.1590/S1678-9946202567031","url":null,"abstract":"<p><p>Mycobacterium marinum is a nontuberculous mycobacterium and an opportunistic pathogen which infects humans. Here, we report a case of an 87-year-old female who had extensive verrucous skin lesions on her upper limbs for 18 months. The patient was diagnosed with Mycobacterium marinum infection by pathology and quantitative polymerase chain reaction (qPCR) assay of skin tissue. After five months of oral rifampicin and clarithromycin, the skin lesions regressed completely.</p>","PeriodicalId":54466,"journal":{"name":"Revista Do Instituto De Medicina Tropical De Sao Paulo","volume":"67 ","pages":"e31"},"PeriodicalIF":1.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammatory markers in cerebrospinal fluid in pediatric Brazilian spotted fever with neurological manifestations.","authors":"Daniela Caldas Teixeira, Pedro Alves Soares Vaz de Castro, Maria Clara de Araujo Gontijo Lima, Roberta Maia de Castro Romanelli, Ana Cristina Simões E Silva, Lilian Martins Oliveira Diniz","doi":"10.1590/S1678-9946202567032","DOIUrl":"10.1590/S1678-9946202567032","url":null,"abstract":"","PeriodicalId":54466,"journal":{"name":"Revista Do Instituto De Medicina Tropical De Sao Paulo","volume":"67 ","pages":"e32"},"PeriodicalIF":1.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allochthonous zoonotic sporotrichosis in the Brazilian Central-West: diagnostic challenges in a nonendemic area.","authors":"Thaís Badini Vieira, Sirlei Franck Thies, Luana Candido Dias, Brenda Mênick de Oliveira, Flávio Silveira, Juliana Maciel Cassali Vieira, Eriana Serpa Barreto, Angélica Cavalheiro Bertagnolli Rodrigues, Mário de Menezes Coppola, Clairton Marcolongo-Pereira, Renata Osório Faria, Angelita Dos Reis Gomes","doi":"10.1590/S1678-9946202567030","DOIUrl":"10.1590/S1678-9946202567030","url":null,"abstract":"<p><p>Sporotrichosis, a neglected zoonotic fungal infection, is becoming increasingly prevalent in Brazil, with cats being the primary source of human transmission. This report details the first documented case of zoonotic human sporotrichosis in Mato Grosso State, a non-endemic area; the infection was acquired from an animal in an endemic area. The patient developed a subcutaneous ulcerative lesion following contact with a cat from Minas Gerais State, a known disease hotspot. Initially misdiagnosed, the infection was later confirmed as Sporothrix brasiliensis after fungal culture and molecular analysis. The patient was successfully treated with itraconazole. This case highlights the importance of considering sporotrichosis in the differential diagnosis, even in non-endemic areas, due to the risk of zoonotic transmission. It also emphasizes the need for a One Health approach to improve surveillance, diagnostic accuracy, and management of emerging fungal diseases in endemic and expanding areas.</p>","PeriodicalId":54466,"journal":{"name":"Revista Do Instituto De Medicina Tropical De Sao Paulo","volume":"67 ","pages":"e30"},"PeriodicalIF":1.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Oropouche fever cases in a Northeastern Brazilian state, April to September 2024.","authors":"Paulo Ricardo Martins-Filho, Thialla Andrade Carvalho, Lucindo José Quintans-Júnior, Adriano Antunes de Souza Araújo, Marco Aurélio de Oliveira Góes, Cliomar Alves Dos Santos","doi":"10.1590/S1678-9946202567027","DOIUrl":"https://doi.org/10.1590/S1678-9946202567027","url":null,"abstract":"<p><p>The recent Oropouche fever outbreak in Sergipe State, Northeastern Brazil, represents a significant expansion of the disease beyond the Amazon. This shift in transmission patterns highlights the urgent need to adapt public health strategies and strengthen surveillance efforts to better understand and manage the spread of the Oropouche virus across diverse ecological and climatic settings.</p>","PeriodicalId":54466,"journal":{"name":"Revista Do Instituto De Medicina Tropical De Sao Paulo","volume":"67 ","pages":"e27"},"PeriodicalIF":1.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}