Ima Fungus最新文献

{"title":"Symbiotic synergy: How Arbuscular Mycorrhizal Fungi enhance nutrient uptake, stress tolerance, and soil health through molecular mechanisms and hormonal regulation.","authors":"Nazir Ahmed, Juan Li, Yongquan Li, Lifang Deng, Lansheng Deng, Muzafaruddin Chachar, Zaid Chachar, Sadaruddin Chachar, Faisal Hayat, Ahmed Raza, Javed Hussain Umrani, Lin Gong, Panfeng Tu","doi":"10.3897/imafungus.16.144989","DOIUrl":"10.3897/imafungus.16.144989","url":null,"abstract":"<p><p>Arbuscular Mycorrhizal (AM) symbiosis is integral to sustainable agriculture and enhances plant resilience to abiotic and biotic stressors. Through their symbiotic association with plant roots, AM improves nutrient and water uptake, activates antioxidant defenses, and facilitates hormonal regulation, contributing to improved plant health and productivity. Plants release strigolactones, which trigger AM spore germination and hyphal branching, a process regulated by genes, such as <i>D27</i>, <i>CCD7</i>, <i>CCD8</i>, and <i>MAX1</i>. AM recognition by plants is mediated by receptor-like kinases (RLKs) and LysM domains, leading to the formation of arbuscules that optimize nutrient exchange. Hormonal regulation plays a pivotal role in this symbiosis; cytokinins enhance AM colonization, auxins support arbuscule formation, and brassinosteroids regulate root growth. Other hormones, such as salicylic acid, gibberellins, ethylene, jasmonic acid, and abscisic acid, also influence AM colonization and stress responses, further bolstering plant resilience. In addition to plant health, AM enhances soil health by improving microbial diversity, soil structure, nutrient cycling, and carbon sequestration. This symbiosis supports soil pH regulation and pathogen suppression, offering a sustainable alternative to chemical fertilizers and improving soil fertility. To maximize AM 's potential of AM in agriculture, future research should focus on refining inoculation strategies, enhancing compatibility with different crops, and assessing the long-term ecological and economic benefits. Optimizing AM applications is critical for improving agricultural resilience, food security, and sustainable farming practices.</p>","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":"16 ","pages":"e144989"},"PeriodicalIF":5.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143755938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pseudobaeosporoideae</i>, a new subfamily within the <i>Tricholomataceae</i> for the genus <i>Pseudobaeospora</i> (<i>Agaricales</i>, <i>Tricholomatineae</i>) based on morphological and molecular inference.","authors":"Alfredo Vizzini, Giovanni Consiglio, Katarína Adamčíková, Ledo Setti, Slavomír Adamčík","doi":"10.3897/imafungus.16.144994","DOIUrl":"10.3897/imafungus.16.144994","url":null,"abstract":"<p><p>Based on molecular and morphological evidence the new subfamily <i>Pseudobaeosporoideae</i> of the <i>Tricholomataceae</i> is established within the <i>Tricholomatineae</i> for accommodating the unique features of <i>Pseudobaeospora</i> such as gymnocarpic mycenoid/collybioid habit, small-sized spores with thick and dextrinoid wall, and presence of crassobasidia. Twenty-six <i>Pseudobaeospora</i> collections corresponding to eleven species (five types) were newly sequenced. Collections morphologically attributable to <i>P.oligophylla</i> (type of the genus) or to <i>P.pillodii</i> are here sequenced for the first time: accordingly, <i>P.oligophylla</i> is considered as a posterior synonym of <i>P.pillodii</i>. Quélet's original plate is selected as a lectotype for <i>Collybiapillodii</i> and a French collection as its epitype collection. <i>Pseudobaeosporadeceptiva</i> is described as a new species from Italy very close to <i>P.pillodii</i> from which it differs mainly by bigger spores and SSU and LSU rDNA sequences. The presence of <i>P.pyrifera</i> in Italy is documented for the first time and <i>P.mutabilis</i> is reduced to its later synonym. A neotype is established for <i>P.jamonii</i> which is here proved to be an independent species. Finally, a critical review of the characters used for interspecific distinctions in <i>Pseudobaeospora</i> was provided.</p>","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":"16 ","pages":"e144994"},"PeriodicalIF":5.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143694345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling fungal diversity associated with coffee trees in China using a polyphasic approach and a global review of coffee saprobic fungi.","authors":"Li Lu, Samantha C Karunarathna, Kunhiraman C Rajeshkumar, Abdallah M Elgorban, Ruvishika S Jayawardena, Sinang Hongsanan, Nakarin Suwannarach, Jaturong Kumla, Yin-Ru Xiong, Kevin D Hyde, Mei-Yan Han, De-Ge Zheng, Qiang Li, Dong-Qin Dai, Saowaluck Tibpromma","doi":"10.3897/imafungus.16.144874","DOIUrl":"10.3897/imafungus.16.144874","url":null,"abstract":"<p><p>Arabica coffee (<i>Coffeaarabica</i>) is the most cultured and popular coffee bean in today's world. Yunnan Province is well known as China's largest arabica coffee cultivation region. Fungi represent an important group of microorganisms associated with coffee, profoundly influencing its yield and quality. In this study, twelve fungal collections growing on dead and decaying twigs of coffee were collected and isolated to systematically document microfungi associated with coffee plants in Yunnan Province. Ten novel species, each representing a unique family within <i>Pleosporales</i>, were identified and introduced, based on comprehensive morphological analyses and multigene phylogenetic studies. The ten new species belong to the families <i>Bambusicolaceae</i>, <i>Didymellaceae</i>, <i>Didymosphaeriaceae</i>, <i>Longiostiolaceae</i>, <i>Lophiostomataceae</i>, <i>Massarinaceae</i>, <i>Neomassariaceae</i>, <i>Occultibambusaceae</i>, <i>Roussoellaceae</i> and <i>Thyridariaceae</i> with each family containing one new species. Macro- and micro-characteristics, descriptions and phylogenetic trees indicating the placement of the new taxa are provided. In addition, pairwise homoplasy index (PHI) test results and morphological comparisons between the new species and closely-related taxa are given. This study also establishes a comprehensive global inventory of saprobic fungi associated with coffee, which is intended to help researchers and professionals worldwide with practical information. This research enhances the understanding of coffee-associated fungal diversity in China and underscores the importance of introducing new saprobic fungal taxa related to coffee.</p>","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":"16 ","pages":"e144874"},"PeriodicalIF":5.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11915015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Fungi</i>: Pioneers of chemical creativity - Techniques and strategies to uncover fungal chemistry.","authors":"Hedda Schrey, Christopher Lambert, Marc Stadler","doi":"10.3897/imafungus.16.142462","DOIUrl":"https://doi.org/10.3897/imafungus.16.142462","url":null,"abstract":"<p><p>Natural product discovery from fungi for drug development and description of novel chemistry has been a tremendous success. This success is expected to accelerate even further, owing to the advent of sophisticated technical advances of technical advances that recently led to the discovery of an unparalleled biodiversity in the fungal kingdom. This review aims to give an overview on i) important secondary metabolite-derived drugs or drug leads, ii) discuss the analytical and strategic framework of how natural product discovery and drug lead identification transformed from earlier days to the present, iii) how knowledge of fungal biology and biodiversity facilitates the discovery of new compounds, and iv) point out endeavors in understanding fungal secondary metabolite chemistry in order to systematically explore fungal genomes by utilizing synthetic biology. An outlook is given, underlining the necessity for a collaborative and cooperative scenario to harness the full potential of the fungal secondary metabolome.</p>","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":"16 ","pages":"e142462"},"PeriodicalIF":5.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fusarioid fungus forms mutualistic interactions with poplar trees that resemble ectomycorrhizal symbiosis.","authors":"Ningning Yang, Xiaoliang Shan, Kexuan Wang, Junkun Lu, Ying Zhu, Redman S Regina, Russell J Rodriguez, Jiajia Yao, Francis M Martin, Zhilin Yuan","doi":"10.3897/imafungus.16.143240","DOIUrl":"https://doi.org/10.3897/imafungus.16.143240","url":null,"abstract":"<p><p><i>Fusarium</i> species, recognised as global priority pathogens, frequently induce severe diseases in crops; however, certain species exhibit alternative symbiotic lifestyles and are either non-pathogenic or endophytic. In this study, we characterised the mutualistic relationship between the eFp isolate of <i>F.pseudograminearum</i> and five poplar species, resulting in formation root structures reminiscent of ectomycorrhizal (ECM) symbiosis. This functional symbiosis is evidenced by enhanced plant growth, reciprocal nutrient exchange, improved nitrogen and phosphorus uptake and upregulation of root sugar transporter gene expression (<i>PtSweet1</i>). Comparative and population genomics confirmed that eFp maintains a structurally similar genome, but exhibits significant divergence from ten conspecific pathogenic isolates. Notably, eFp enhanced the growth of diverse plant lineages (<i>Oryza</i>, <i>Arabidopsis</i>, <i>Pinus</i> and non-vascular liverworts), indicating a near-complete loss of virulence. Although this specialised symbiosis has only been established <i>in vitro</i>, it holds significant value in elucidating the evolutionary track from endophytic to mycorrhizal associations.</p>","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":"16 ","pages":"e143240"},"PeriodicalIF":5.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel genus and species of <i>Diaporthostomataceae</i> (<i>Diaporthales</i>) in China.","authors":"Ning Jiang, Han Xue, Yong Li","doi":"10.3897/imafungus.16.145422","DOIUrl":"https://doi.org/10.3897/imafungus.16.145422","url":null,"abstract":"<p><p><i>Diaporthales</i> is a significant fungal order comprising species that predominantly inhabit plant tissues, being pathogens, endophytes, and saprobes. Recent studies have uncovered extensive species diversity across various hosts, utilizing both morphological characteristics and molecular phylogenetic analyses. In this study, samples of leaf spots and branch cankers were collected from China, and fungal isolations were established. Species identification was conducted using a phylogenetic approach based on combined sequence data from the internal transcribed spacer (ITS) region, large subunit ribosomal DNA (LSU), the DNA-directed RNA polymerase II second largest subunit (<i>rpb2</i>), and translation elongation factor 1-alpha (<i>tef1</i>) genes, together with morphological observations. As a result, the novel genus <i>Tiania</i> is proposed, with three newly described species: <i>T.chinensis</i>, <i>T.lithocarpicola</i>, and <i>T.quercicola</i>. These species are validated by pairwise homoplasy index (PHI) analysis, ensuring robust support for their distinction. This study explores the rare family <i>Diaporthostomataceae</i>, providing the first descriptions of their anamorphic forms. By offering detailed morphological and molecular data, this research lays a foundation for future taxonomic and systematic studies of the <i>Diaporthales</i>.</p>","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":"16 ","pages":"e145422"},"PeriodicalIF":5.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Nigromargaritatarda</i> gen. et sp. nov. and distribution of an intron position class within <i>Pleosporales</i>.","authors":"Meng-Yuan Li, Xiang Sun, Yu-Qing Liu, Sheng-Hui Qin, Min Li, Xue-Li He","doi":"10.3897/imafungus.16.145425","DOIUrl":"10.3897/imafungus.16.145425","url":null,"abstract":"<p><p><i>Pleosporales</i>, the largest order in <i>Dothideomycetes</i>, has a broad host range and inhabits host plants as epiphytes, endophytes, parasites and saprophytes. <i>Trematosphaeriaceae</i> is a monophyletic family in <i>Pleosporales</i>, composed of species of deviated ecological background and morphological traits. In this study, we described a new fungal taxon under <i>Trematosphaeriaceae</i>, based on root endophytic fungi recovered from the desert plant <i>Gymnocarposprzewalskii</i> in Gansu Province, China. The taxon is characterised by simple, aseptate conidia and pycnidia in unusually small sizes. Multilocus phylogenetic analysis, based on ITS, LSU, SSU and TEF sequences and a morphology study indicated that the taxon represented a new genus within the <i>Trematosphaeriaceae</i> and was named <i>Nigromargaritatarda</i>. Intriguingly, an intron of 355 bp in length located at site 453 on the ribosomal SSU gene was detected in one strain of <i>N.tarda</i>. Sequence analysis and phylogenetic analysis indicated that the intron belongs to an intron position class (Pcl) restricted to <i>Pleosporales</i>. Phylogeny affiliated distribution of this Pcl was confined at the genus or lower level, suggesting a horizontal transmission pattern of this Pcl. This study established a new genus in <i>Trematosphaeriaceae</i> and depicted the spread features of a less-documented Pcl amongst <i>Pleosporales</i> families with high resolution, which promotes our understanding of the origin and transmission mechanism of such mobile genetic elements.</p>","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":"16 ","pages":"e145425"},"PeriodicalIF":5.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The six whole mitochondrial genomes for the <i>Diaporthe</i> species: features, evolution and phylogeny.","authors":"Shunpei Xie, Xuyang Ma, Haiyan Wu, Rui Zang, Haiqiang Li, Miao Liu, Qiang Li, Qingzhou Ma, Yashuang Guo, Meng Zhang","doi":"10.3897/imafungus.16.140572","DOIUrl":"10.3897/imafungus.16.140572","url":null,"abstract":"<p><p>In this study, the complete mitogenomes of three <i>Diaporthe</i> species (<i>Diaportheeres</i> ZM79-3, <i>D.phaseolorum</i> ZM33-4 and <i>Diaporthe</i> sp. ZM41-5) were sequenced, assembled and compared with the other three previously sequenced <i>Diaporthe</i> mitogenomes (<i>D.caulivora</i> VNIIKR SE Dcaul3, <i>D.longicolla</i> MSPL 10-6 and <i>D.sojae</i> VNIIKR SE Dps12). The six <i>Diaporthe</i> mitogenomes were found to be circular DNA molecules, with lengths ranging from 53,646 bp to 108,865 bp. The mitogenomes of the six <i>Diaporthe</i> species mainly comprised the same set of 15 core protein-coding genes (PCGs), two rRNAs, and a certain number of tRNAs and unidentified open reading frames (ORFs). The PCG length, AT skew and GC skew showed large variability among the 15 PCGs in the six mitogenomes. The <i>nad1</i> gene had the least K2P genetic distance of the 15 core PCGs among the 13 <i>Diaporthales</i> species, indicating that this gene was highly conserved. The Ka/Ks values for all 15 core PCGs were < 1, suggesting that these genes were all subject to purifying selection. Comparative mitogenome analysis showed that introns contributed the most to the size variation of <i>Diaporthe</i> mitogenomes. Frequent intron loss/gain events were detected to have occurred in the <i>cox1</i> gene during the evolution of the <i>Diaporthales</i> mitogenomes. Although the mitogenomes of 13 species from <i>Diaporthales</i> had undergone large-scale gene rearrangements, six mitogenomes of <i>Diaporthe</i> species had identical gene arrangements. Phylogenetic analysis based on combined mitochondrial gene datasets showed that the six <i>Diaporthe</i> species formed well-supported topologies. To our knowledge, this study is the first report on the mitogenomes of <i>D.phaseolorum</i> ZM33-4 and <i>Diaporthe</i> sp. ZM41-5, as well as the first comparison of mitogenomes among <i>Diaporthe</i> species. Our findings will further promote investigations of the genetics, evolution and phylogeny of the <i>Diaporthe</i> species.</p>","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":"16 ","pages":"e140572"},"PeriodicalIF":5.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-distance gene flow and recombination shape the evolutionary history of a maize pathogen.","authors":"Flávia Rogério, Cock Van Oosterhout, Stéphane De Mita, Francisco Borja Cuevas-Fernández, Pablo García-Rodríguez, Sioly Becerra, Silvia Gutiérrez-Sánchez, Andrés G Jacquat, Wagner Bettiol, Guilherme Kenichi Hosaka, Sofia B Ulla, Jürg Hiltbrunner, Rogelio Santiago, Pedro Revilla, José S Dambolena, José L Vicente-Villardón, Ivica Buhiniček, Serenella A Sukno, Michael R Thon","doi":"10.3897/imafungus.16.138888","DOIUrl":"10.3897/imafungus.16.138888","url":null,"abstract":"<p><p>The evolutionary history of crop pathogens is shaped by a complex interaction of natural and anthropogenic factors. The fungus <i>Colletotrichumgraminicola</i> causes maize anthracnose which results in significant yield losses worldwide. We conducted a comprehensive investigation into the evolutionary genomics of <i>C.graminicola</i> using a collection of 212 isolates from 17 countries across five continents. Genomic analyses supported the existence of three geographically isolated genetic lineages, with a significant pattern of isolation by distance. We identified two distinct gene flow patterns, driven by short- and long-distance dispersal, likely resulting from the natural spread of the pathogen and the exchange of contaminated seeds. We present evidence of genetic introgression between lineages, suggesting a long history of recombination. We identified significant recombination events coalescing at distinct points in time, with the North American lineage displaying evidence of the most ancient recombination. Demographic modelling has indicated that North America is an intermediate between Brazil, Europe and an ancestral, unsampled source population, which is hypothesised to be Mesoamerican. Our analyses revealed that the global genomic structure of <i>C.graminicola</i> is shaped by geographic differentiation driven by long-distance migration and a long history of recombination and introgression. We show historical relationships amongst these lineages, identifying a potential route for fungal spread, with the North American population emerging ancestrally, followed sequentially by the Brazilian and European populations. Our research indicates that the European lineage is more virulent, which has implications for the potential emergence of new outbreaks of maize anthracnose in Europe.</p>","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":"16 ","pages":"e138888"},"PeriodicalIF":5.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation and comparative analysis of mitochondrial genomes of false, yellow, black and blushing morels provide insights on their structure and evolution.","authors":"Gang Tao, Steven Ahrendt, Shingo Miyauchi, XiaoJie Zhu, Hao Peng, Kurt Labutti, Alicia Clum, Richard Hayes, Patrick S G Chain, Igor V Grigoriev, Gregory Bonito, Francis M Martin","doi":"10.3897/imafungus.16.138363","DOIUrl":"10.3897/imafungus.16.138363","url":null,"abstract":"<p><p><i>Morchella</i> species have considerable significance in terrestrial ecosystems, exhibiting a range of ecological lifestyles along the saprotrophism-to-symbiosis continuum. However, the mitochondrial genomes of these ascomycetous fungi have not been thoroughly studied, thereby impeding a comprehensive understanding of their genetic makeup and ecological role. In this study, we analysed the mitogenomes of 30 <i>Morchellaceae</i> species, including yellow, black, blushing and false morels. These mitogenomes are either circular or linear DNA molecules with lengths ranging from 217 to 565 kbp and GC content ranging from 38% to 48%. Fifteen core protein-coding genes, 28-37 <i>tRNA</i> genes and 3-8 <i>rRNA</i> genes were identified in these <i>Morchellaceae</i> mitogenomes. The gene order demonstrated a high level of conservation, with the <i>cox1</i> gene consistently positioned adjacent to the <i>rnS</i> gene and <i>cob</i> gene flanked by <i>apt</i> genes. Some exceptions were observed, such as the rearrangement of <i>atp6</i> and <i>rps3</i> in <i>Morchellaimportuna</i> and the reversed order of <i>atp6</i> and <i>atp8</i> in certain morel mitogenomes. However, the arrangement of the <i>tRNA</i> genes remains conserved. We additionally investigated the distribution and phylogeny of homing endonuclease genes (HEGs) of the LAGLIDADG (LAGs) and GIY-YIG (GIYs) families. A total of 925 LAG and GIY sequences were detected, with individual species containing 19-48HEGs. These HEGs were primarily located in the <i>cox1</i>, <i>cob</i>, <i>cox2</i> and <i>nad5</i> introns and their presence and distribution displayed significant diversity amongst morel species. These elements significantly contribute to shaping their mitogenome diversity. Overall, this study provides novel insights into the phylogeny and evolution of the <i>Morchellaceae</i>.</p>","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":"16 ","pages":"e138363"},"PeriodicalIF":5.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881001/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}