International Journal for Parasitology-Parasites and Wildlife最新文献

{"title":"Ectoparasites of the European wildcat (Felis silvestris) in Germany","authors":"Katrin Bisterfeld ,&nbsp;Marie-Kristin Raulf ,&nbsp;Andrea Springer ,&nbsp;Johannes Lang ,&nbsp;Michael Lierz ,&nbsp;Christina Strube ,&nbsp;Ursula Siebert","doi":"10.1016/j.ijppaw.2024.100977","DOIUrl":"10.1016/j.ijppaw.2024.100977","url":null,"abstract":"<div><p>Understanding the impact of parasites on wildlife populations is an important aspect of conservation management. However, research on ectoparasites in wildlife can be difficult, as examinations of live animals which are not habituated to human handling are often impossible. The European wildcat (<em>Felis silvestris</em>) is a strictly protected wildlife species whose population has been recovering in Germany in recent decades. Several studies from different European countries have investigated the parasitological status of European wildcat populations. However, most of these studies assessed endoparasite infections, whereas ectoparasite infestations have often been neglected. To fill this knowledge gap for wildcats in Germany, 131 dead found specimens were examined for ectoparasites by macroscopic and microscopic examination of the fur and the ear canals. Infestation with ectoparasites was present in 84.0% (110/131) of the wildcats. Ticks showed the highest prevalence with 72.5% (95/131) of wildcats infested, with 49.6% (65/131) infested with <em>Ixodes ricinus</em> and 36.6% (48/131) with <em>Ixodes hexagonus</em>/<em>canisuga</em>. A total of 27.5% (36/131) of the wildcats were positive for at least one flea species. Of the nine different flea species identified by morphology and/or molecular analyses, Ceratophyllidae were most common (16.8% [22/131]), with <em>Ceratophyllus sciurorum</em> confirmed on 12.2% (16/131) and <em>Nosopsyllus fasciatus</em> on 1.5% (2/131) animals, followed by <em>Pulex irritans</em> (5.3% [7/131]), <em>Spilopsyllus cuniculi</em> (3.8% [5/131]), <em>Chaetopsylla</em> spp. (3.1% [4/131]) (2/131 <em>Chaetopsylla trichosa</em> and 1/131 <em>Chaetopsylla globiceps</em>), <em>Ctenocephalides felis</em> (1.5% [2/131]), <em>Archaeopsylla erinacei</em> (1.5% [2/131]) and <em>Ctenophthalmus baeticus</em> (0.8% [1/131]). Further, 23.7% (31/131) of the wildcats harboured mites, identified as <em>Trombicula autumnalis</em> in 12.2% (16/131) and <em>Otodectes cynotis</em> in 4.8% (6/124) of cases. The only louse species detected was <em>Felicola hercynianus</em> with a prevalence of 2.3% (3/131). Infestation intensities ranged from 1 to 86 ticks, 1–49 fleas, 1–1896 mites, and 1–92 <em>F. hercynianus</em> per wildcat. This study demonstrates that a variety of ectoparasites infests wildcats in Germany, but they do not seem to have a serious impact on the general health of wildcats, as judged by the hosts' mostly good or very good nutritional condition. In addition, the potential risk to domestic cats (<em>Felis catus</em>) and humans posed by the wildcats’ ectoparasites, appears to be low but present.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"25 ","pages":"Article 100977"},"PeriodicalIF":2.0,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000737/pdfft?md5=ef8ab052c75594513213c919a6fb3b0e&pid=1-s2.0-S2213224424000737-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142136305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights from common buzzard broods on the interaction between Leucocytozoon infection, watercourse habitats and simuliid blackfly vectors","authors":"Anja Wiegmann ,&nbsp;Andrea Springer ,&nbsp;Meinolf Ottensmann ,&nbsp;Tony Rinaud ,&nbsp;Oliver Krüger ,&nbsp;Christina Strube ,&nbsp;Nayden Chakarov","doi":"10.1016/j.ijppaw.2024.100978","DOIUrl":"10.1016/j.ijppaw.2024.100978","url":null,"abstract":"<div><p>Blood parasites of the genus <em>Leucocytozoon</em> commonly infect many bird species worldwide and are particularly prevalent in birds of prey. As a vector-borne parasitic disease, the infection occurrence overlaps with that of the dominant vectors: blackflies (Diptera, Simuliidae). These blood-sucking insects are dependent on habitats with flowing freshwaters for the development of their larval stages. We investigated the correlation between the proximity to flowing waters and <em>Leucocytozoon</em> infection probability in common buzzard (<em>Buteo buteo</em>) broods, as well as the occurrence of adult blackflies directly at the nests. In addition, we investigated the survival of captured simuliids in relation to host infection intensity. In total in 2019, we examined 112 different nests, including 297 common buzzard nestlings, with a <em>Leucocytozoon</em> prevalence of 56.6% among the nestlings and of 80.3% at brood level. We found no significant association of <em>Leucocytozoon</em> infection probability with nestling age, the distance to the nearest stream and the sum of the length of streams within a radius of 200 and 1000 m around each nest. The number of blackflies caught around the nest showed a tentative correlation with the probability of <em>Leucocyozoon</em> infection of the nestlings. Among the subsample of 218 blackfly individuals that survived day one after capture, survival averaged 6.2 days. Our results suggest that <em>Leucocytozoon</em> transmission is complex and requires consideration of many factors, related to habitat and vector prevalence, especially given their temporal variation.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"25 ","pages":"Article 100978"},"PeriodicalIF":2.0,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000749/pdfft?md5=f1872811366a10ec361f669ec02f9ae1&pid=1-s2.0-S2213224424000749-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142076073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of Babesia and Hepatozoon species and morphological characteristics of Babesia species in Japanese wild boars","authors":"Shiho Ohmori ,&nbsp;Motoko Nagano-Fujii ,&nbsp;Kazuo Suzuki ,&nbsp;Masataka Korenaga ,&nbsp;Fumi Murakoshi ,&nbsp;Atsuko Saito-Ito","doi":"10.1016/j.ijppaw.2024.100975","DOIUrl":"10.1016/j.ijppaw.2024.100975","url":null,"abstract":"<div><p>We investigated intraerythrocytic <em>Babesia</em> parasites in 21 Japanese wild boars, <em>Sus scrofa leucomystax</em>, captured in Wakayama Prefecture on the mainland from 2008 to 2009 and in 31 Japanese wild boars from 2011 to 2013 in Kochi Prefecture on Shikoku Island, Japan. We detected small subunit ribosomal RNA (18S rRNA) gene (SSUrDNA) fragments of a <em>Babesia</em> species in 17 boars from Wakayama and 18 boars from Kochi. The nearly full SSUrDNA sequence (1669 bps) of this species was determined. A FASTA search revealed that the SSUrDNA sequence of the <em>Babesia</em> sp. in Japanese wild boars was the most homologous to those of several <em>Babesia</em> isolates reported as <em>Babesia gibsoni</em>. Phylogenetic analysis showed that the <em>Babesia</em> sp. found in Japanese wild boars was the closest relative to <em>B. gibsoni</em> but made a different clade from <em>B. gibsoni</em>. The <em>Babesia</em> sp. in Japanese wild boars was completely different from <em>Babesia</em> sp. Suis found in a European domestic pig, <em>Sus scrofa domesticus</em>. By microscopic examination, ring-shaped, oval and pear-shaped small sized intraerythrocytic parasites were observed on blood smears of 12 of 18 Japanese wild boars whose blood smears could be examined in Wakayama. We also detected SSUrDNA fragments of a <em>Hepatozoon</em> species in 6 of the 21 wild boars from Wakayama. The nearly full SSUrDNA sequence (1774 bps) of the <em>Hepatozoon</em> sp. was shown to be identical to that of <em>Hepatozoon apri</em>.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"25 ","pages":"Article 100975"},"PeriodicalIF":2.0,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000713/pdfft?md5=e4ce270951389e8ed57a981d7cca6ce9&pid=1-s2.0-S2213224424000713-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142151234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges for assessment of cohabiting stocks of argentine shortfin squid Illex argentinus using parasites as biological tags","authors":"María Paz Gutiérrez ,&nbsp;Delfina Canel ,&nbsp;Paola E. Braicovich ,&nbsp;Ana L. Lanfranchi ,&nbsp;Manuel M. Irigoitia ,&nbsp;Marcela L. Ivanovic ,&nbsp;Nicolás I. Prandoni ,&nbsp;Beatriz Elena ,&nbsp;Juan T. Timi","doi":"10.1016/j.ijppaw.2024.100974","DOIUrl":"10.1016/j.ijppaw.2024.100974","url":null,"abstract":"<div><p>The Argentine shortfin squid <em>Illex argentinus</em> is one of the most important commercial species for the Argentine fisheries. The understanding of its stock structure is therefore necessary to ensure fishery sustainability and, given the relevance of squids in the regional food web, for biodiversity conservation. An overlap between parasitology and fisheries lies in the use of parasites as biological tags to identify the stock composition of exploited resources, however, the efficiency of this methodology has been questioned for stock assessment in cephalopods. In this work, the value of parasite assemblages of <em>I. argentinus</em> to discriminate between the co-occurring summer spawning stock (SSS) and south patagonic stock (SPS) in a mixing area over the Patagonian continental shelf during summer was evaluated for two cohorts. Five shortfin squid samples corresponding to SSS and SPS were examined for metazoan parasites. The squid size affected the parasite assemblage similarities, conversely, no gender effect on the infracommunities was observed. Multivariate analysis evidenced similarity in parasite assemblage composition and structure between both stocks captured in the mixing area on the same date. This similarity was related to the presence of short-lived trophically transmitted parasites, which are associated with their recently consumed food items and, indirectly, to the oceanographic conditions. The same set of host and environmental variables were identified as the most probable causes of the temporal variability observed in parasite assemblages between SPS cohorts and even intra-cohort. Despite the value of parasites as tags for discriminating squid stocks may have little value when cohabiting stocks are analysed, their variability could serve as a valuable indicator of environmental conditions. The use of parasites as biological tags to discriminate stocks needs to be verified at different spatiotemporal scales, including samples from other non-sympatric stocks in the analyses.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"25 ","pages":"Article 100974"},"PeriodicalIF":2.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000701/pdfft?md5=9b19e74c1ebe41a6d71c4f3514df56d4&pid=1-s2.0-S2213224424000701-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142044497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal dynamics in gastrointestinal helminth infections of sympatric mouse lemur species (Microcebus murinus and Microcebus ravelobensis) in Northwestern Madagascar","authors":"Annette Klein ,&nbsp;Ute Radespiel ,&nbsp;Andrea Springer ,&nbsp;Romule Rakotondravony ,&nbsp;Christina Strube","doi":"10.1016/j.ijppaw.2024.100972","DOIUrl":"10.1016/j.ijppaw.2024.100972","url":null,"abstract":"<div><p>Madagascar's lemur populations are declining in dwindling habitats due to anthropogenic expansion and changing climatic conditions. Gastrointestinal parasites can be important indicators to assess the health status of threatened species. However, parasites, hosts and the environment are connected in complex interactions. The present study aimed to disentangle the impact of seasonal and several host-specific factors (sex, species, age, reproductive status, and body mass) on endoparasitism in two small-bodied, co-occurring lemur species (<em>Microcebus murinus</em> and <em>Microcebus ravelobensis</em>) in the Ankarafantsika National Park. Helminth prevalence and egg shedding intensity was investigated via copromicroscopic examination of 810 fecal samples that were obtained from 178 individuals across an 11-month period with a longitudinal approach via repeated captures in a 30.6 ha forest area. Both mouse lemur hosts shed seven morphologically distinct egg types (assigned to <em>Subulura baeri</em>, unidentified Enterobiinae, <em>Spirura</em> sp., <em>Lemuricola</em> sp., two Hymenolepididae spp., one unidentified ascarid). Postmortem examination of two deceased individuals enabled assignment of adult worms to egg morphotypes of <em>S. baeri</em>, <em>Spirura</em> sp. and one Hymenolepididae sp., supported by molecular analysis. A significant seasonal variation was observed in the occurrence of the three most common helminth species <em>S</em>. <em>baeri</em> (total prevalence 71%), unidentified Enterobiinae (46%) and <em>Spirura</em> sp. (38%), with a higher likelihood of infection with advancing dry season. Neither host species, sex nor reproductive status had a significant effect on gastrointestinal helminth infections. Host body mass showed pronounced seasonal changes but did not differ significantly between infected and non-infected individuals. The pathogenic effects of gastrointestinal helminths therefore likely remained within compensable limits in the studied mouse lemur populations. Our findings highlight the prominent influence of seasonal changes on helminth communities. The results of combined morphologic and genetic approaches can furthermore help to overcome limitations of parasite identification via copromicroscopy by linking egg morphology to DNA sequences.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"25 ","pages":"Article 100972"},"PeriodicalIF":2.0,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000683/pdfft?md5=3d8da184945ba253f3041341192cd52b&pid=1-s2.0-S2213224424000683-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and ecological relationships of Cestoda and Monogenoidea parasites of freshwater stingrays (Myliobatiformes, Potamotrygonidae), in the upper Paraná River, Brazil","authors":"Jumma Miranda Araújo Chagas ,&nbsp;Douglas de Castro Ribeiro ,&nbsp;Thalita Fischer Santini Mendes ,&nbsp;Felipe Chinaglia Montefeltro ,&nbsp;Luciano Alves dos Anjos","doi":"10.1016/j.ijppaw.2024.100968","DOIUrl":"10.1016/j.ijppaw.2024.100968","url":null,"abstract":"<div><p>The Neotropical freshwater stingrays of <em>Potamotrygon</em> genus present a unique and complex natural history and biogeographical pattern that can be traced to a marine origin and the colonization of the continental environment during the Miocene. During the evolution of potamotrygonids, several species of the parasitic fauna coevolved and co-opted concomitantly to their hosts during the colonization of the new environments. One striking example can be observed during the colonization of the upper Paraná River region. However, few studies explored the ecological and taxonomic aspects of potamotrygonid parasites. In this work, we investigate aspects of the ecology and taxonomy of the species of Monogenea and Cestoda that are parasites the species of freshwater stingrays of the genus <em>Potamotrygon</em> in the upper Paraná River. Our results indicate that at least six species of parasites are present in potamotrygonids in the region. Two of the observed parasites are putative new species and three of the parasitic species were identified for the first time in the region, hence expanding their geographic distributions. We quantified ecological aspects at different levels of communities for the collected parasite species. We compared the diversity in different locations and hosts and performed an exploratory analysis to investigate the differences in parasite abundance. Additionally, an identification key for the Monogenea and Cestoda species of the sampled region is provided.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"24 ","pages":"Article 100968"},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000646/pdfft?md5=bf3b953f80bf3d18c2a1cc18da58828d&pid=1-s2.0-S2213224424000646-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141838750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double trouble: Co-infection of Angiostrongylus vasorum and Dirofilaria immitis in golden jackal (Canis aureus) in Friuli Venezia Giulia, Italy","authors":"Massimo Orioles ,&nbsp;Daniele Fabbri ,&nbsp;Giovanna Miani ,&nbsp;Stefano Pesaro ,&nbsp;Luca Dorigo ,&nbsp;Marco Bregoli ,&nbsp;Elena Saccà ,&nbsp;Paolo Tomè ,&nbsp;Paola Beraldo","doi":"10.1016/j.ijppaw.2024.100969","DOIUrl":"10.1016/j.ijppaw.2024.100969","url":null,"abstract":"<div><p>This study reports the first co-infection of <em>Angiostrongylus vasorum</em> and <em>Dirofilaria immitis,</em> two nematodes affecting canid cardiopulmonary systems, in golden jackals (<em>Canis aureus</em>) in Italy. Data on golden jackal carcasses, recovered in Friuli Venezia Giulia (Italy) from 2020 to 2023, were recorded using InfoFaunaFVG wildlife monitoring network. Out of 60 necropsied golden jackals, 24 tested positive for either pathogen, with 3 animals displaying co-infection. Pathological findings included verminous pneumonia, abdominal and thoracic bleeding, and adult individuals in the heart and pulmonary arteries. The recent expansion of the golden jackal in northern Italy may favour the establishment and spread of these nematodes, posing challenges for disease containment and surveillance. Given the increasing prevalence of angiostrongylosis and cardiopulmonary dirofilariosis in Europe, health monitoring of wild canids is essential to reduce their potential impact.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"24 ","pages":"Article 100969"},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000658/pdfft?md5=5bf17b3cc5024a8aa7a2d862bfc9b175&pid=1-s2.0-S2213224424000658-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of Babesia coryicola sp. nov. from Florida pumas (Puma concolor coryi) from southern Florida, USA","authors":"Barbara C. Shock ,&nbsp;Håkon H. Jones ,&nbsp;Kayla B. Garrett ,&nbsp;Sonia M. Hernandez ,&nbsp;Holly J. Burchfield ,&nbsp;Katie Haman ,&nbsp;Helen Schwantje ,&nbsp;Sam R. Telford ,&nbsp;Mark W. Cunningham ,&nbsp;Michael J. Yabsley","doi":"10.1016/j.ijppaw.2024.100963","DOIUrl":"10.1016/j.ijppaw.2024.100963","url":null,"abstract":"<div><p>Previously, a high prevalence of piroplasms has been reported from Florida pumas (<em>Puma concolor coryi)</em> from southern Florida. In the current study, we describe the biological characteristics of a novel <em>Babesia</em> species in Florida pumas. Ring-stage trophozoites were morphologically similar to trophozoites of numerous small babesids of felids including <em>B. leo, B. felis</em>, and <em>Cytauxzoon felis</em>. Parasitemias in Florida pumas were very low (&lt;1%) and hematologic values of 25 <em>Babesia-</em>infected Florida pumas were within normal ranges for <em>P. concolor</em>. Phylogenetic analysis of near full-length 18S rRNA gene, β-tubulin, cytochrome <em>c</em> oxidase subunit I, cytochrome <em>c</em> oxidase subunit III, and cytochrome <em>b</em> gene sequences indicated that this <em>Babesia</em> species is a member of the <em>Babesia</em> sensu stricto clade and is related to groups of <em>Babesia</em> spp. from carnivores or ungulates, although the closest group varied by gene target. Internal transcribed spacer (ITS)-1 region sequences from this <em>Babesia</em> sp. from 19 Florida pumas were 85.7–99.5% similar to each other and ∼88% similar to <em>B. odocoilei.</em> Similarly, an ITS-2 sequence from one puma was 96% similar to <em>B. bigemina</em> and 92% similar to a <em>Babesia</em> sp. from a red panda (<em>Ailurus fulgens</em>). Infected pumas were positive for antibodies that reacted with <em>B. odocoilei</em>, <em>B. canis,</em> and <em>B. bovis</em> antigens with titers of 1:256, 1:128, and 1:128, respectively. No serologic reactivity was noted for <em>Theileria equi</em>. No molecular evidence of congenital infection was detected in 24 kittens born to 11 <em>Babesia</em>-infected female pumas. Pumas from other populations in the United States [Louisiana (n = 1), North Dakota (n = 5) and Texas (n = 28)], British Columbia, Canada (n = 9), and Costa Rica (n = 2) were negative for this <em>Babesia</em> sp. Collectively, these data provide morphologic, serologic, genetic, and natural history data for this novel <em>Babesia</em> sp. which we propose the name <em>Babesia</em> coryicola sp. nov. sp. This is the first description of a felid-associated <em>Babesia</em> species in North America.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"24 ","pages":"Article 100963"},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000592/pdfft?md5=1eb3491be68e60808fb2c62abab4374d&pid=1-s2.0-S2213224424000592-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141693816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of Cryptosporidium parvum in wild rodents (Phyllotis darwini) inhabiting protected and rural transitional areas in north-central Chile","authors":"Patricio D. Carrera-Játiva ,&nbsp;Gerardo Acosta-Jamett ,&nbsp;Pamela Muñoz","doi":"10.1016/j.ijppaw.2024.100971","DOIUrl":"10.1016/j.ijppaw.2024.100971","url":null,"abstract":"<div><p>Wild rodents often harbor <em>Cryptosporidium</em> species that can be transmitted to multiple mammal hosts. In Chile, little is known about <em>Cryptosporidium</em> in wild rodents, and available studies have been focused on morphological findings with no molecular-based evidence. A longitudinal survey was conducted between 2021 and 2022 to investigate the occurrence of <em>Cryptosporidium</em> spp. in populations of the Darwin's leaf-eared mouse (<em>Phyllotis darwini</em>) living in protected and rural transitional areas in north-central Chile, using staining and molecular methods. A total of 247 fecal samples were collected and examined by the modified Ziehl–Neelsen (ZN) staining test, 54 of which were positive for <em>Cryptosporidium</em>-like oocysts. Molecular analyses were carried out by PCR of the partial 18S ribosomal RNA and 60 kDa glycoprotein (<em>gp60</em>) genes. <em>Cryptosporidium</em> infection was confirmed in 34 samples (13.7 %) based on the PCR amplification, and individual (i.e., sex, and body mass index) and ecological variables (i.e., type of site and season) were not statistically significant (p &gt; 0.05). Using the nucleotide sequencing of the partial 18S rRNA gene, <em>Cryptosporidium parvum</em> was identified in nine isolates. Also, <em>C. parvum</em> subgenotype family <em>IIa</em> was determined in seven samples by the partial <em>gp60</em> gene, including the subtype <em>IIaA17G4R1</em> in two samples<em>.</em> This is the first molecular evidence of <em>Cryptosporidium parvum IIa</em> in <em>Phyllotis darwini</em> in Chile. These results indicate potential cross-species transmition between wild rodents and domestic-wild animals in north-central Chile. More research is needed to understand better the role of wild rodents in the transmission of <em>Cryptosporidium</em> spp. in Chile.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"24 ","pages":"Article 100971"},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000671/pdfft?md5=0c9d82badc2ec5ba82e546a136780348&pid=1-s2.0-S2213224424000671-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Outbreak of parasite-induced limb malformations in a declining amphibian species in Colorado” [Int. J. Parasitol.: Parasites Wildl. 24, August (2024), 100965]","authors":"Pieter T.J. Johnson ,&nbsp;Dana M. Calhoun ,&nbsp;Tyler J. Achatz ,&nbsp;Stephen E. Greiman ,&nbsp;Adrian Gestos ,&nbsp;William H. Keeley","doi":"10.1016/j.ijppaw.2024.100970","DOIUrl":"10.1016/j.ijppaw.2024.100970","url":null,"abstract":"","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"24 ","pages":"Article 100970"},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S221322442400066X/pdfft?md5=3449cbaf1b03f3a9d605e90d996da7cd&pid=1-s2.0-S221322442400066X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}