Journal of Genetic Engineering and Biotechnology最新文献

{"title":"Unveiling the association of sirtuin1 (SIRT1) gene polymorphisms rs3758391 and rs12778366 with the risk, clinicopathological characteristics and susceptibility of breast cancer in Bangladeshi population","authors":"Fariya Aboni,&nbsp;Md Rasel Khan Manik,&nbsp;Zimam Mahmud,&nbsp;Sonia Tamanna,&nbsp;Abdullah Al Saba,&nbsp;Farzana Ansari,&nbsp;Md. Zakir Hossain Howlader","doi":"10.1016/j.jgeb.2026.100833","DOIUrl":"10.1016/j.jgeb.2026.100833","url":null,"abstract":"<div><div>Breast cancer (BC) is one of the most prevalent malignancies and a leading cause of cancer-related mortality among women worldwide. Genetic variation in the SIRT1 (Sirtuin-1) gene has been implicated in cancer susceptibility; however, evidence regarding its association with breast cancer in the Bangladeshi population remains limited. This study aimed to investigate the association of SIRT1 polymorphisms (rs3758391 and rs12778366) with breast cancer susceptibility and clinicopathological characteristics, while also evaluating demographic and hematological parameters among Bangladeshi women. A hospital-based case-control study was conducted involving 199 histopathologically confirmed breast cancer patients and 100 healthy controls. Genotyping of rs3758391 and rs12778366 was performed using the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) technique. Logistic regression analysis was employed to estimate crude and age-adjusted odds ratios (ORs) with 95% confidence intervals (CIs). Herein, significant differences in genotype distributions were observed between cases and controls for both polymorphisms. For rs3758391, individuals carrying the TT genotype showed significantly higher odds of breast cancer under the codominant model (adjusted OR = 4.30, 95% CI: 1.60–11.55, <em>p</em> = 0.004) and recessive model (adjusted OR = 4.76, 95% CI: 1.80–12.59, <em>p</em> = 0.0003) after adjustment for age. In contrast, although the TC + CC genotype of rs12778366 demonstrated increased odds in the dominant model before adjustment (OR = 1.76, <em>p</em> = 0.038), the association lost statistical significance after age adjustment (<em>p</em> = 0.06). Haplotype analysis revealed a significant global association before age adjustment, which became non-significant after adjustment. Among clinicopathological characteristics, rs12778366 TC genotype was significantly associated with progesterone receptor positivity (OR = 3.20, 95% CI: 1.3–8.12, <em>p</em> = 0.012), while no associations were observed with tumor stage. Hematological analysis demonstrated significantly lower hemoglobin levels and elevated erythrocyte sedimentation rate (ESR), neutrophil percentage, plateletcrit (PCT), and mean platelet volume (MPV) in breast cancer patients compared with controls. In conclusion, SIRT1 rs3758391 may serve as a potential genetic susceptibility marker for breast cancer in Bangladeshi women, whereas the association of rs12778366 appears less robust after age adjustment. Larger studies and functional investigations are warranted to validate these findings.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100833"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149199781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of GSTM1 and GSTT1 gene polymorphisms with prostate Cancer risk in men of African descent: A systematic review and Meta-analysis","authors":"Itunuoluwa Isewon,&nbsp;Micheal Anthony,&nbsp;Jerry Emmanuel,&nbsp;Jelili Oyelade","doi":"10.1016/j.jgeb.2026.100834","DOIUrl":"10.1016/j.jgeb.2026.100834","url":null,"abstract":"<div><div>Prostate cancer (PCa) disproportionately affects men of African descent, and glutathione S-transferase Mu 1 (GSTM1) alongside glutathione S-transferase Theta 1 (GSTT1) have been reported to be potential genetic contributors due to their role in detoxification pathways. However, evidence on their association with PCa risk in African-origin populations remains limited. Therefore, this systematic review and meta-analysis evaluated the association between GSTM1 and GSTT1 polymorphisms and PCa risk in men of African descent. Following PRISMA guidelines, seven case-control studies published between 2006 and 2023 were included, with a total of 873 PCa cases and 1121 controls from North African, West African, Afro-Caribbean, and African-American populations. Random-effects models were used to calculate pooled effect sizes, with heterogeneity assessed using the I² statistic. The results suggest that, for GSTM1, neither the null genotype nor the positive genotype demonstrated a significant association with PCa risk, a pattern that was consistent across populations. Funnel plot assessments and Egger's regression tests (<em>p</em> = 0.6876) were consistent with an absence of publication bias for GSTM1, although the small number of studies limits the power of these tests. However, GSTT1 analyses revealed considerable variability. While the null genotype showed no overall association, substantial heterogeneity (I² = 68.3%) was observed. Visual inspection of funnel plots suggested potential asymmetry, but Egger's test (<em>p</em> = 0.5032) did not provide statistical evidence of publication bias, although this assessment is underpowered given the small number of studies. Notably, North African studies reported increased risk with the GSTT1 null genotype, whereas the Caribbean cohort showed a protective effect. The positive genotype also displayed conflicting trends, with protective effects in North Africa and elevated risks in Caribbean populations. These region-specific GSTT1 patterns should be regarded as hypothesis-generating, and the findings overall suggest that genetic risks may vary between populations and highlight the need for ancestry-focused studies that also consider environmental and lifestyle factors to better understand prostate cancer in men of African origin.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100834"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149199782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of HOTAIR gene polymorphisms and biochemical markers to uterine fibroids in Egyptian women.","authors":"Mona M. Gad,&nbsp;Hossam Abdel-Megeed Abdou,&nbsp;Magdy M. Youssef","doi":"10.1016/j.jgeb.2026.100797","DOIUrl":"10.1016/j.jgeb.2026.100797","url":null,"abstract":"<div><h3>Background</h3><div>Uterine fibroids (UFs) are the most common benign tumors of the female reproductive system, with a multifactorial etiology involving genetic, hormonal, and inflammatory mechanisms. Long non-coding RNA HOX transcript antisense intergenic RNA (HOTAIR) has been implicated in tumorigenesis; however, the role of its genetic polymorphisms in uterine fibroids remains insufficiently explored.</div></div><div><h3>Aim</h3><div>This study aimed to investigate the association between HOTAIR gene polymorphisms (rs1899663, rs12826786, and rs4759314) and susceptibility to uterine fibroids in Egyptian women, as well as their relationship with inflammatory, hormonal, and biochemical parameters.</div></div><div><h3>Methods</h3><div>A total of 200 participants were enrolled, including 100 uterine fibroid patients and 100 age-matched healthy controls. Genotyping of HOTAIR polymorphisms was performed using amplification refractory mutation system polymerase chain reaction (ARMS-PCR). Serum cytokines, hormonal markers, and biochemical parameters were measured using ELISA and standard clinical methods. Statistical analyses were conducted to evaluate genotype–phenotype associations and disease risk.</div></div><div><h3>Results</h3><div>The rs12826786 polymorphism showed a significant association with uterine fibroid susceptibility, with the T allele being more frequent in patients than controls (45.5% vs. 33.5%, OR = 1.657, <em>p</em> = 0.019). Significant associations were also observed under the dominant and codominant genetic models (<em>p</em> = 0.026). In contrast, rs1899663 and rs4759314 polymorphisms showed no significant association. Uterine fibroid patients exhibited significantly elevated levels of inflammatory cytokines (IL-1β, IL-10, TNF-α, IFN-γ), hormonal markers (prolactin and estradiol), and liver enzymes (ALT, AST, ACP), along with decreased total protein and albumin levels (<em>p</em> &lt; 0.001). A significant association between rs12826786 and ALT levels was also observed (<em>p</em> = 0.038). Principal component analysis demonstrated clustering patterns influenced by inflammatory and hormonal parameters.</div></div><div><h3>Conclusion</h3><div>This study demonstrates that the HOTAIR rs12826786 polymorphism is significantly associated with increased susceptibility to uterine fibroids in Egyptian women, potentially through modulation of inflammatory and hormonal pathways. These findings highlight the potential role of lncRNA polymorphisms as biomarkers for uterine fibroid risk. However, further studies in larger and diverse populations are required to validate these findings.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100797"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148856003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic protective effects of testosterone and propolis against cisplatin-induced hepatic-renal dysfunction in male rats: a biochemical and histological study","authors":"Assal G. Alshammary,&nbsp;Farah Mahmood Shteeb,&nbsp;Zainab Rasheed Hameed,&nbsp;Alaa Lateef Ali,&nbsp;Rusul A.A. Alshammary","doi":"10.1016/j.jgeb.2026.100808","DOIUrl":"10.1016/j.jgeb.2026.100808","url":null,"abstract":"<div><h3>Background</h3><div>Cancer remains one of the leading causes of mortality worldwide, with a continuously rising incidence. While cisplatin (Cis) is a widely used chemotherapeutic drug in cancer management, its clinical application is severely limited by chemotherapy –induced side effects, particularly hepatic and renal toxicity.</div></div><div><h3>Objective</h3><div>The study aimed to evaluate the protective effect of propolis and testosterone against cisplatin- induced hepatic-renal damage in male rats.</div></div><div><h3>Methods</h3><div>Thirty adult male white rats (200 and 220 g) were randomly divide into five groups (n = 6/group): (1) negative control group<span><span>2</span></span>; positive control group receiving cisplatin (7 mg/kg, IP single dose)<span><span>3</span></span>; testosterone and cisplatin (25 mg/kg, IP).<span><span>4</span></span> Propolis and cisplatin (250 mg/kg propolis, orally);(5) testosterone, propolis and cisplatin (combined treatment).</div></div><div><h3>Results</h3><div>The serum in cisplatin treated group revealed a significant increase in creatinine, urea, ALT, AST and ALP. Moreover, significant decrease in SOD, and GSH and an increase MDA level in serum rat in comparison with control group. In addition. DNA damage was assessed in rat blood cells using alkaline comet assay. Administration of testosterone alone or propolis alone partially improved hepatic-renal damage and reduced DNA damage, while the combination of both showed superior protective effects. Histological examinations showed the hepatic renal damage that induced by cisplatin was attenuated by testosterone and propolis.</div></div><div><h3>Conclusion</h3><div>these results suggested that propolis and testosterone, whether alone or in combination may effectively attenuate cisplatin- induced hepatotoxicity and nephrotoxicity, potentially through their antioxidant and anti-inflammatory properties.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100808"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148953450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative structure–activity relationships of green-synthesized silver, zinc oxide, and selenium nanoparticles mediated by Peganum harmala: physicochemical characteristics and multifunctional biological activities","authors":"Nouf Alharbi,&nbsp;Madyha H. Mahmoud,&nbsp;Eman Merghani A. Mohammed,&nbsp;Soha Taymour,&nbsp;Karim Abdelsalam,&nbsp;Fatemah Refaei,&nbsp;Ibrahim Shanyah,&nbsp;Mona M. Khalifa","doi":"10.1016/j.jgeb.2026.100815","DOIUrl":"10.1016/j.jgeb.2026.100815","url":null,"abstract":"<div><h3>Background</h3><div>An environmentally responsible route to sustainable nanomaterial production is provided by green synthesis, enabling multifunctional bioactive systems intended for potential biomedical applications. Plant-mediated processes can reduce cost and environmental burden relative to conventional nanoparticle fabrication methods; phytochemical surface capping may also strengthen biological functionality in the resulting materials. In this study, <em>Peganum harmala</em> L. seed extract, a medicinal plant rich in flavonoids, phenolics, and terpenoids, was used as the plant source for biosynthesis.</div></div><div><h3>Methods</h3><div>Silver nanoparticles (Ag-NPs), zinc oxide nanoparticles (ZnO-NPs), and selenium nanoparticles (Se-NPs) were synthesized using <em>Peganum harmala</em> extract as a natural reducing and stabilizing agent. The synthesized nanoparticles were characterized by UV–visible spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), and zeta potential analysis. Their biological activities were evaluated through a series of <em>in vitro</em> assays, including antioxidant capacity (total antioxidant capacity, reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), and nitric oxide scavenging (NO), carbohydrate-metabolizing enzyme (α-amylase, α-glucosidase, and aldose reductase),</div></div><div><h3>Results</h3><div>The three nanoparticle systems exhibited distinct, pathway-specific biological activities consistent with their physicochemical profiles. ZnO-NPs (PDI = 0.184; zeta = +23.1 mV) demonstrated superior antioxidant and anti-diabetic enzyme inhibitory activities, with the lowest IC₅₀ values across DPPH, ABTS, α-amylase, α-glucosidase, and aldose reductase assays. Se-NPs (PDI = 0.091; zeta = +19.8 mV) exhibited the strongest anti-inflammatory (cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), and 5-lipoxygenase (5-LOX) and anti-arthritic activities, with IC₅₀ values approaching those of standard drugs. All three nanoparticles showed moderate, comparable cholinesterase inhibition. Ag-NPs consistently showed the weakest performance across most pathways, potentially related to their lower zeta potential (−14.4 mV) and reliance on steric rather than electrostatic stabilization.</div></div><div><h3>Conclusions</h3><div>The synthesized nanoparticles exhibited distinct bio functional profiles governed by elemental composition and physicochemical characteristics. ZnO-NPs were predominantly associated with antioxidant and anti-diabetic activities, whereas Se-NPs demonstrated superior anti-inflammatory and anti-arthritic properties. These findings highlight <em>P. harmala</em>-mediated nanoparticle synthesis as a sustainable and versatile platform for developing multifunctional nanomaterials with promising biomedical applications.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 4","pages":"Article 100815"},"PeriodicalIF":2.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148953471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Osteogenic enhancement of modular ceramic nanocomposites impregnated with human dental pulp stem cells: an approach for bone repair and regenerative medicine” [J. Genet. Eng. Biotechnol. 20(1) (2022) 123]","authors":"Eman E.A. Mohammed,&nbsp;Hanan H. Beherei,&nbsp;Mohamed El–Zawahry,&nbsp;Abdel Razik H. Farrag,&nbsp;Naglaa Kholoussi,&nbsp;Iman Helwa,&nbsp;Mostafa Mabrouk,&nbsp;Alice K. Abdel Aleem","doi":"10.1016/j.jgeb.2026.100666","DOIUrl":"10.1016/j.jgeb.2026.100666","url":null,"abstract":"","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 2","pages":"Article 100666"},"PeriodicalIF":2.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148274240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Isolation and characterization of chitinolytic fast-growing bacteria from marine environment in Vietnam”. [J. Genet. Eng. Biotechnol. 23 (2025) 100587]","authors":"Thi Van Anh Tran,&nbsp;Thuy Hang Dam,&nbsp;Lan Huong Nguyen,&nbsp;Tuan Le,&nbsp;Thi Kieu Oanh Vu,&nbsp;Thomas Walther,&nbsp;Ha Thanh Le","doi":"10.1016/j.jgeb.2025.100648","DOIUrl":"10.1016/j.jgeb.2025.100648","url":null,"abstract":"","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 2","pages":"Article 100648"},"PeriodicalIF":2.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148274190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Evaluation of ARHGAP26 expression, prognostic significance, and immune infiltration in clear cell renal cell carcinoma” [J. Genet. Eng. Biotechnol. 23(4) (2025) 100593]","authors":"Aichun Long,&nbsp;Tianyun Li,&nbsp;Cheng Huang,&nbsp;Yuwei Shi,&nbsp;Cuiwei Zhang","doi":"10.1016/j.jgeb.2025.100652","DOIUrl":"10.1016/j.jgeb.2025.100652","url":null,"abstract":"","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 2","pages":"Article 100652"},"PeriodicalIF":2.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148274195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational discovery of marine natural phytochemicals as novel SIRT7 inhibitors for cancer treatment","authors":"K.M. Tanjida Islam,&nbsp;Roksana Khanam,&nbsp;Shahin Mahmud","doi":"10.1016/j.jgeb.2025.100654","DOIUrl":"10.1016/j.jgeb.2025.100654","url":null,"abstract":"<div><div>With cancer causing over 10 million deaths annually, identifying novel therapeutic targets is crucial. SIRT7 is a NAD-dependent deacetylase that regulates oncogenic pathways, making it a promising therapeutic target. However, no approved medications currently exist against SIRT7, and available inhibitors exhibit limited efficacy alongside significant toxicity. Therefore, this study explores marine biodiversity as a source of SIRT7 inhibitors. Molecular docking screening of SIRT7 identified four promising marine phytochemicals (CMNPD28383, CMNPD24305, CMNPD24304, CMNPD14924) with superior binding affinities (−9.9 to −8.4 kcal/mol). Molecular dynamics simulations confirmed stable protein–ligand complexes with RMSD variations of 4.714 to 6.905 Å. Most of these phytochemicals demonstrated favorable ADMET profiles, high oral bioavailability, strong predicted anticancer activity (Pa &gt; 0,6), and potent machine learning (RandomForest Cross Validation R² = 0.8614 ± 0.0244) predicted inhibitory activity (pIC₅₀: −2.110 to −2.875; IC₅₀: 129 nM to 749 nM). Therefore, these marine phytochemicals could be potential novel SIRT7 inhibitors with excellent drug-like properties, providing a foundation for further experimental validation and potential clinical translation. In addition, these computational approaches offer a promising avenue to develop safer and effective cancer therapeutics from marine sources.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100654"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, characterization, and expression profiling of genes encoding tubby-like proteins in rice exposed to realistic environmental contamination of anilofos and bentazone","authors":"Zhi Jiang He ,&nbsp;Yang Yang Mo ,&nbsp;Xiao Lu Wang ,&nbsp;Zhi Zhong Zhou ,&nbsp;Yan Hui Wang ,&nbsp;Li Qing Zeng ,&nbsp;Ying Yu Zeng ,&nbsp;Xiao Liang Liu ,&nbsp;Xuesheng Li ,&nbsp;Zhao Jie Chen","doi":"10.1016/j.jgeb.2026.100680","DOIUrl":"10.1016/j.jgeb.2026.100680","url":null,"abstract":"<div><div>Tubby-like proteins, which are encoded by large multigene families in plants, play important roles in abiotic stress tolerance; however, it is still unclear how they work in rice during herbicides stress. In order to address this gap, we looked into the traits and roles of genes encoding tubby-like proteins in rice plants (<em>Oryza sativa</em>; hereafter referred to as <em>OsTLP</em> genes) exposed to two herbicides, anilofos and bentazone. Transcriptome analysis revealed 7 genes encoding tubby-like proteins. Quantitative reverse-transcription PCR confirmed that the expression of 6 and 4 <em>TLP</em> genes was enhanced under anilofos- and bentazone-induced stress, respectively. Seven genes were found to be unevenly distributed over five of the twelve chromosomes, with segmental duplication playing a role in the growth of gene families, according to chromosomal mapping. According to a collinearity research, rice and other plant species have the following orthologous gene pairs: 17 with sorghum (<em>Sorghum bicolor</em>), 0 with Arabidopsis (<em>Arabidopsis thaliana</em>), 11 with maize (<em>Zea mays</em>), and 7 with soybean (<em>Glycine max</em>). These genes were divided into three clades by phylogenetic analysis. According to structural study, <em>TLP</em> genes have a variety of conserved domains, motif compositions, <em>cis</em>-acting elements, and designs that allow them to respond to both biotic and abiotic stress. Analysis of protein–protein interaction networks further showed that tubby-like proteins may contribute to anilofos and bentazone metabolism. According to in silico predictions of subcellular localization, all seven <em>TLP</em> genes’ encoded proteins are found in the nucleus. Docking analyses identified important amino acid residues implicated in pesticide binding between tubby-like proteins and the two herbicides (anilofos and bentazone). These results shed light on the <em>TLP</em> gene superfamily and provide useful resources for functional research on these genes’ functions in herbicides metabolism.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"24 1","pages":"Article 100680"},"PeriodicalIF":2.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147395713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}