Journal of Genetic Engineering and Biotechnology最新文献

{"title":"Exploring the anticancer and antioxidant properties of Lagerstroemia speciosa bark extract via phytochemical and molecular docking analysis","authors":"Shahnaz Parvin Sweety ,&nbsp;Tahsin Ahmed Rupok ,&nbsp;Mst. Shahnaj Parvin ,&nbsp;Jaytirmoy Barmon ,&nbsp;Mst. Sarmina Yeasmin ,&nbsp;Md. Ekramul Islam","doi":"10.1016/j.jgeb.2025.100553","DOIUrl":"10.1016/j.jgeb.2025.100553","url":null,"abstract":"<div><div><em>Lagerstroemia speciosa</em> is traditionally used for treating diabetes and inflammation; however, its anticancer potential remains unexplored. This study assesses the antioxidant and anticancer activities of <em>L. speciosa</em> bark extract, with a focus on Ehrlich Ascites Carcinoma (EAC) cells in mice. The crude methanol extract (CME) was subdivided into n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), and aqueous (AQF) fractions. In vitro antioxidant assays identified CHF as the most active fraction, exhibiting high phenolic and flavonoid content. CHF and EAF were further investigated for anticancer activity in an EAC-induced mouse model, where CHF significantly (<em>P</em> &lt; 0.05) reduced tumor cell count compared to EAF. Phytochemical characterization using FTIR and GC–MS revealed bioactive compounds, including 9-Methoxybicyclo[6.1.0]nona-2,4,6-triene and 9,12-Octadecadienoic acid methyl ester (E,E). Molecular docking studies demonstrated strong interactions between these compounds and key cancer-related proteins, p53 and Topoisomerase-II, suggesting potential anticancer mechanisms. Overall, this study shows that <em>L. speciosa</em> bark extract has therapeutic potential, especially for CHF. Bioactive compounds like 9,12-octadecadienoic acid methyl ester (E,E) and 9-methoxybicyclo [6.1.0]nona-2,4,6-triene contribute to the extract’s antioxidant and anticancer effects in EAC models, possibly by modulating p53 and Topoisomerase-II.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100553"},"PeriodicalIF":2.8,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dumpsite-isolated Bacillus safensis Z1 with protease yield for potential industrial use","authors":"Zahra Abbass Farroukh ,&nbsp;Jamilah Borjac ,&nbsp;Dalia El Badan","doi":"10.1016/j.jgeb.2025.100543","DOIUrl":"10.1016/j.jgeb.2025.100543","url":null,"abstract":"<div><div>Proteases, particularly those derived from microbial sources, have become indispensable in various industries due to their cost-effectiveness, versatility, and sustainability. They provide a more efficient and environmentally friendly alternative to traditional animal- and plant-based enzymes. In this regard, water samples collected from the Sidon dump site were screened for their ability to produce protease. The isolates showed positive results on skim milk agar and were therefore selected as protease-producing strains. The isolates were tested on skim milk agar plates. Of the 6 isolated strains, the most potent isolate was identified as <em>Bacillus safensis Z1</em>. Optimized parameters (time, pH, temperature) for maximum protease activity and microbial growth of <em>B. safensis Z1</em> include 48 h, pH 7, at 40 °C. The enzyme was homogeneously purified by salt precipitation. SDS-PAGE confirmed that the isolated enzyme had a molecular weight of approximately 50 kDa. It was significantly inhibited by PMSF, indicating that it belongs to serine protease family. The enzyme’s tolerance with surfactants and commercial detergents indicates its potential application in the detergent industry. Furthermore, the partially purified enzyme demonstrated stain removal and feather disintegration capabilities.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100543"},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Licoisoflavone B and glabridin from Glycyrrhiza glabra as potent nucleoprotein antagonists of Lassa virus: insights from molecular docking, dynamics simulation, PCA, and DFT studies","authors":"Sk. Faisal Ahmed ,&nbsp;Md. Masudur Rahman Munna ,&nbsp;Md. Hossain Ahmed ,&nbsp;Md. Mostafizur Rahman ,&nbsp;Minhajul Islam ,&nbsp;Esha Mony Bristy","doi":"10.1016/j.jgeb.2025.100544","DOIUrl":"10.1016/j.jgeb.2025.100544","url":null,"abstract":"<div><div>Lassa virus causes a severe hemorrhagic disease referred to as Lassa fever. It exhibits a significant mortality rate among people in West and Central Africa. Currently, there is no vaccine available, and ribavirin is the sole treatment option with significant limitations. Given the lack of an effective medication, this study explores bioactive phytocompounds from the plant <em>Glycyrrhiza glabra</em> that may be safer and more effective than ribavirin in combating viruses’ nucleoprotein activity, which is essential for the replication of viruses and host immune evasion. Our virtual screening and multi-stage molecular docking analyses of 69 natural phytochemicals from this plant revealed the compounds licoisoflavone B and glabridin as potential therapeutics. These compounds exhibit strong binding affinities toward the target protein, with superior ADMET profiles. Both compounds also maintained structural stability throughout 100 ns molecular dynamics simulations, while principal component analysis further corroborated their conformational stability, highlighting potential efficiency. Furthermore, density functional theory analysis indicated favorable electronic properties, supporting the compounds’ potential as viable drug candidates. These findings suggested licoisoflavone B and glabridin as potential therapeutic candidates for Lassa fever. However, this study underscores the urgency of further experimental validation to advance these compounds toward novel anti-Lassa virus therapeutics.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100544"},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting Streptococcus pyogenes atpF protein for multi-epitope vaccine development: a genomics-driven immunoinformatics strategy","authors":"Manisha Agarwal ,&nbsp;Sanjeeb Handique ,&nbsp;Sanchaita Rajkhowa ,&nbsp;Abhichandan Das ,&nbsp;Debashis Panda ,&nbsp;Sami A. Al-Hussain ,&nbsp;Magdi E.A. Zaki","doi":"10.1016/j.jgeb.2025.100546","DOIUrl":"10.1016/j.jgeb.2025.100546","url":null,"abstract":"<div><div><em>Streptococcus pyogenes</em>, a medium-priority pathogen on the WHO’s 2024 Bacterial Pathogen Priority List, is a major cause of infectious disease-related mortality. The increasing prevalence of antibiotic resistance, coupled with the absence of a licensed vaccine due to the pathogen’s genetic diversity and autoimmune concerns, underscores the need for novel therapeutic strategies. This study employs reverse vaccinology and subtractive proteomics to design a multi-epitope vaccine targeting atpF, a conserved extracellular protein essential for ATP synthesis. The atpF protein was identified based on its high antigenicity and functional importance in <em>S. pyogenes</em>. Three vaccine constructs (SM1, SM2, and SM3) were designed by integrating antigenic B-cell and T-cell epitopes with immune-modulating adjuvants and linkers. Physicochemical and immunological assessments confirmed their stability, solubility, and antigenicity. Molecular modeling identified SM1 as the most promising candidate, demonstrating superior immune receptor binding affinity and flexible epitope interactions, facilitating effective antibody recognition. <em>In silico</em> immune simulations further demonstrated SM1′s potential to elicit strong humoral and cellular immune responses, while codon optimization confirmed efficient expression in <em>E. coli</em>. These findings introduce atpF as a promising vaccine target and highlight SM1′s potential as a viable vaccine candidate. However, experimental validation is essential to confirm its efficacy, safety, and immunogenicity <em>in vivo</em>. This study underscores the role of computational modeling in accelerating vaccine development, providing a strategic alternative to traditional approaches.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100546"},"PeriodicalIF":2.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simplified, one step technique for disinfection of non-hardened rainbow trout eggs with tosylchloramide (Chloramine T) and peroxide (Wofasteril) compounds and the effects on bacterial load and microbiome composition in comparison to iodophore disinfection","authors":"Franz Lahnsteiner ,&nbsp;Anna Dünser","doi":"10.1016/j.jgeb.2025.100541","DOIUrl":"10.1016/j.jgeb.2025.100541","url":null,"abstract":"<div><div>Disinfection of the interior of non-hardened eggs with iodophors (Buffodine®) is an established hygienic practice in salmonid aquaculture to prevent pathogen transmission from the broodstock fish to their offspring. As iodophors inhibit sperm motility, fertilization is first performed in a 0.75 % NaCl solution, followed by egg disinfection in a second step after fertilization is complete. Although this two-step egg disinfection procedure is simple to perform under laboratory conditions, it presents challenges for fish farms using mass stripping. The process involves two highly time-sensitive steps, requiring precise execution, as any errors can lead to fertilization failure or ineffective disinfection. A more practical approach would be to simplify disinfection into a single-step procedure. The present study demonstrates that non-hardened rainbow trout eggs can be fertilized and disinfected simultaneously using a one-step method with tosylchloramide (100 mg/l Chloramine T®) or peroxide (100 µl Wofasteril®) compounds in 0.75 % NaCl for 40 min. This procedure is feasible as the applied concentrations of Chloramine T and Wofasteril have only low impact on sperm motility. The one-step methods do also not negatively impact embryo and early larval development. Non-hardened rainbow trout egg disinfection methods with Chloramine T and Wofasteril were as effective as the conventional Buffodine method in reducing total bacterial load of eggs at 3 h post-fertilization (hpf), the point at which water hardening is complete. Reanalysis of total bacterial load after 22 days of development (embryo stage at the onset of eye pigmentation) proved Chloramine T more effective than Wofasteril and Buffodine. Microbiome composition differed significantly across developmental stages and disinfection treatments. Notable variations were observed between non-disinfected controls and eggs treated with Buffodine, Chloramine T, or Wofasteril, in persistent bacterial communities, stage-specific bacteria, and bacteria colonizing the chorion during embryogenesis. Buffodine treatment increased bacterial diversity, while Chloramine T and Wofasteril led to reduced diversity compared to the control. These findings are discussed in the context of microbiome stability and resilience, key factors for long-term fish health. In summary, the simplified one-step disinfection protocol offers practical advantages in reducing time-sensitive handling steps and improving efficiency in large-scale aquaculture operations. Moreover, it is environmentally preferable as both Wofasteril and Chloramine T are used at lower concentrations than Buffodine and degrade rapidly in water, leaving no harmful residues.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100541"},"PeriodicalIF":3.5,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of silver nanoparticles using Pyrostegia venusta extract, characterization and estimation of antioxidant, antiglycation and anti-aging activities","authors":"Regildo Márcio Gonçalves da Silva ,&nbsp;Pedro Augusto Pereira Rosatto ,&nbsp;Isabelly do Nascimento Pereira ,&nbsp;Laura Camargo Zibordi ,&nbsp;Hugo Henrique Santos ,&nbsp;Filipe Oliveira Granero ,&nbsp;Célia Cristina Malaguti Figueiredo ,&nbsp;Patrícia Soares Santiago ,&nbsp;Carlos Augusto Prata Gaona ,&nbsp;Nilson Nicolau-Junior ,&nbsp;Luciana Pereira Silva","doi":"10.1016/j.jgeb.2025.100539","DOIUrl":"10.1016/j.jgeb.2025.100539","url":null,"abstract":"<div><div>Pyrostegia venusta presents phytoconstituents with biological properties including antioxidant activity. This study aimed to prepare <em>P. venusta</em> aqueous extract (PvAE), synthesize silver nanoparticles (AgNPs) through green synthesis using PvAE, determine their phytoconstituents, evaluate their in vitro antioxidants, antiglycation, and anti-aging activities, and analyze in silico docking of the main phenolic compounds. Phytoconstituents were identified by HPLC and determined by total polyphenols and flavonoids. AgNPs were characterized by scanning transmission electron microscopy and dynamic light scattering. Evaluation of antioxidant potential was performed by DPPH radical scavenging, FRAP, TBARS, ORAC, and oxidative hemolysis analysis. Evaluation of antiglycation activity was carried out by the determination of free amino groups, inhibition of AGEs formation, and REM assays. Anti-aging pandal was evaluated by in vitro and in silico assays. PvAE exhibited the highest total polyphenols (325.78 mg gallic acid equivalent g⁻¹), while AgNPs showed the highest total flavonoids (373.53 mg rutin equivalent g⁻¹). PvAE presented the highest antioxidant activity in DPPH test (74.90 %), although AgNPs exhibited antioxidant activity in ORAC, FRAP (862 μM Trolox equivalent g⁻¹), TBARS (53.97 % inhibition of TBARS formation), and oxidative hemolysis inhibition (85.36 %) assays. Antiglycation evaluation demonstrated the activity of PvAE and AgNPs. PvAE presented in vitro enzymes inhibition and in silico studies demonstrated possible active binding sites between P. venusta main compounds and enzymes that may be correlated with their potential to act at pre- and post-transcriptional levels. Therefore, this study provides information regarding P. venusta and demonstrates that further studies are needed to evaluate its application.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100539"},"PeriodicalIF":3.5,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144704405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers","authors":"Roshida Soraisham ,&nbsp;Punshi Tongbram ,&nbsp;Surendrakumar Singh Thingnam ,&nbsp;Boris Aheibam ,&nbsp;John Zothanzama ,&nbsp;Dinamani Singh Lourembam ,&nbsp;Robert Thangjam","doi":"10.1016/j.jgeb.2025.100540","DOIUrl":"10.1016/j.jgeb.2025.100540","url":null,"abstract":"<div><div>Characterization and identification of banana into their correct genome groups has been a problematic issue based on morphological and unappropriated molecular markers. In the present study, the genetic relationship between and among 28 banana (<em>Musa</em> spp.) accessions representing 5 genome groups (AAA, BB, AAB, ABB and AB) were evaluated using sequence-related amplified polymorphism (SRAP) and inter simple sequence repeat (ISSR) markers. Although similar genetic relationship parameters were observed with the two markers, the higher values were generated with SRAP profiles as indicated with mean gene diversity value of 0.69 with SRAP and 0.64 with ISSR, polymorphic information content (PIC) of 0.64 with SRAP and 0.59 with ISSR, total genetic diversity (<em>Ht</em>) of 0.39 with SRAP and 0.34 with ISSR, gene diversity within population (<em>Hs</em>) of 0.19 with SRAP and 0.12 with ISSR, coefficient of gene differentiation (<em>Gst</em>) value of 0.51 with SRAP and 0.62 with ISSR and gene flow (<em>Nm</em>)value of 0.51 with SRAP and 0.31 with ISSR. The resulting dendrogram and the population structure also supports and showed the concurrence with the genetic relationship parameters based on the genome groups of the accessions studied. The present finding, also indicates the higher efficacy of SRAP markers over the ISSR markers in the discrimination of banana accessions based on their genome groups by grouping the banana accessions belonging to the different genome groups and ploidy levels. The present finding could be utilized for effective discrimination of banana accessions belonging to different genome groups.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100540"},"PeriodicalIF":3.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A causal relationship between circulating immune cells, plasma metabolites, and pulmonary diseases: a mediated Mendelian randomization study","authors":"Chenyi Zhao ,&nbsp;Xuefei Song ,&nbsp;Dan Yang ,&nbsp;Chenbing Lv ,&nbsp;Yuhan Li ,&nbsp;Abualgasim Elgaili Abdalla ,&nbsp;Tingyu Shi ,&nbsp;Guirong Wang ,&nbsp;Longxiang Xie","doi":"10.1016/j.jgeb.2025.100537","DOIUrl":"10.1016/j.jgeb.2025.100537","url":null,"abstract":"<div><h3>Background</h3><div>Immune cells and plasma metabolites may play important roles in the development of pulmonary diseases, but the relationship between different immune cells, plasma metabolites and various pulmonary diseases is still unclear. In this study, we aim to employ Mendelian randomization (MR) to investigate the causality between immune cells, pulmonary diseases and plasma metabolites.</div></div><div><h3>Methods</h3><div>We analyzed immune cells and seven pulmonary diseases, including interstitial lung disease (ILD), idiopathic pulmonary fibrosis (IPF), lung cancer, pneumonia, chronic obstructive pulmonary disease (COPD), sleep apnea syndrome (SAS), and tuberculosis (TB), using genome-wide association analysis (GWAS) data for immune cells as an exposure factor and seven lung diseases as outcomes. Plasma metabolites GWAS data served as mediators. We applied MR analysis to explore the relationship between immune cells and pulmonary diseases, followed by two-step mediation analysis to identify potential metabolites that may mediate this association.</div></div><div><h3>Results</h3><div>As shown in the results, immune cells contribute to disease progression by reducing the protective effect of metabolites on disease or enhancing the promoting effect of metabolites on disease. These include CD4/CD8br and lung cancer, CD62L-CD86+ myeloid DC AC and Pneumonia, and Naive DN (CD4-CD8-) %T cell and COPD. On the other hand, immune cells suppress disease by increasing the inhibitory effect of metabolites on disease or decreasing the promoting effect of metabolites on disease, These include CD39 on CD39+ CD8br and ILD, CD28+ CD45RA+ CD8br AC and TB, Activated &amp; secreting Treg AC and SAS, HLA DR on DC and IPF.</div></div><div><h3>Conclusions</h3><div>We clarify the importance of the potential mechanisms pertaining to immune cells, metabolites, and pulmonary diseases, highlighting the complex interactions among these factors. This understanding may assist in the diagnosis and treatment of patients with pulmonary diseases.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100537"},"PeriodicalIF":3.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling genetic diversity in pointed gourd (Trichosanthes dioica) genotypes from India’s Eastern plateau and hill region: Insights from morphological and molecular markers","authors":"Ankit Kumar Sinha ,&nbsp;P. Bhavana ,&nbsp;A.K. Singh ,&nbsp;J.K. Ranjan ,&nbsp;H. Choudhary ,&nbsp;G.P. Mishra ,&nbsp;K. Thamilarsi ,&nbsp;Paresh Chaukhande ,&nbsp;Reshma Shinde ,&nbsp;Prakash Kumar ,&nbsp;Jitendra Rajak ,&nbsp;Sajiya Ekbal","doi":"10.1016/j.jgeb.2025.100542","DOIUrl":"10.1016/j.jgeb.2025.100542","url":null,"abstract":"<div><div>Pointed gourd (<em>Trichosanthes dioica</em> Roxb.), a nutritionally rich, dioecious cucurbit crop native to India, holds immense potential for enhancing food security, yet its genetic diversity remains underexplored, limiting breeding efforts for improved yield and quality. This study addresses this gap by evaluating the genetic diversity of 46 pointed gourd genotypes using morphological traits and ISSR markers. Principal component analysis (PCA) revealed three principal components explaining 72.53% of the total morphological variation, with fruit weight (0.47), pulp weight (0.46), and fruit volume (0.44) as primary contributors to PC1, and total fruit yield (0.53) and number of fruits per plant (0.49) dominating PC2. The PCA biplot identified five distinct genotype groups, highlighting significant diversity. Molecular analysis with sixteen polymorphic ISSR markers generated 96 bands, of which 76 were polymorphic (78.91%), with Polymorphism Information Content (PIC) ranging from 0.35 to 0.47. Cluster analysis grouped genotypes into seven morphological and two primary molecular clusters, the latter further divided into six sub-clusters. Swarna Rekha, Swarna Suruchi, Swarna Alaukik, HAP 24, HAP 78, and HAP 113 were identified as highly diverse. These findings demonstrate that integrating morphological and molecular markers effectively uncovers genetic variability, providing a robust foundation for breeding programs. Future work will focus on leveraging these diverse genotypes for hybridization to develop high-yielding, nutrient-rich cultivars and exploring additional molecular markers to further refine genetic diversity assessments.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100542"},"PeriodicalIF":3.5,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro and bioinformatic studies of bioactive compounds from Oceanimonas sp. JM-AZM31 and Lysinibacillus fusiformis JM-AZM37 of sponge-associated marine bacteria from a mangrove habitat in Southeast Sulawesi","authors":"Jendri Mamangkey ,&nbsp;Corrina Lailatul Fadjri ,&nbsp;Sunarto ,&nbsp;Apon Zaenal Mustopa ,&nbsp;Dwi Suryanto ,&nbsp;Nabila Swarna Puspa Hermana ,&nbsp;Nur Arfa Yanti ,&nbsp;Kusmiati Kusmiati ,&nbsp;Herman Irawan ,&nbsp;Adrian Hartanto ,&nbsp;La Ode Adi Parman Rudia ,&nbsp;Rizna Akmaliyah ,&nbsp;Lucas William Mendes ,&nbsp;Ferdin","doi":"10.1016/j.jgeb.2025.100538","DOIUrl":"10.1016/j.jgeb.2025.100538","url":null,"abstract":"<div><div>The ongoing quest for novel therapeutic agents has directed attention toward bioactive compounds derived from sponge-associated bacteria. This study focuses on sponge symbiont bacteria from the mangrove ecosystems in Tanjung Tiram, Southeast Sulawesi, which have not yet been reported for their potential antibacteria, anti-inflammatory, antioxidant, and anti-diabetic activities. The screening of marine bacterial isolates was performed using a series of assays: disc diffusion method to assess antibacterial activity, protein denaturation to assess anti-inflammatory properties, DPPH free radical scavenging to evaluate antioxidant capacity, and α-Glucosidase inhibition for anti-diabetic activity, followed by <em>in silico</em> validation. Two promising strains, identified through molecular techniques were designated as <em>Oceanimonas</em> sp. JM-AZM31 and <em>Lysinibacillus fusiformis</em> JM-AZM37. Initial bioactivity screening revealed significant potential: The bacterial isolates JM-AZM31 and JM-AZM37 demonstrated broad-spectrum antibacterial activity against both Gram-positive pathogens (<em>Bacillus cereus, Staphylococcus aureus, Staphylococcus epidermidis</em>) and Gram-negative pathogens (<em>Escherichia coli, Salmonella typhimurium</em>). JM-AZM31 exhibited an anti-inflammatory inhibition rate of 76.9 ± 2.90 %, antioxidant activity of 80.3 ± 1.02 %, and anti-diabetic activity of 84.9 ± 0.49 %. Similarly, JM-AZM37 showed anti-inflammatory activity of 71.6 ± 1.85 %, antioxidant activity of 76.9 ± 0.03 %, and anti-diabetic activity of 83.2 ± 1.27 %. Further analysis using GC–MS identified five significant compounds, which were examined through in silico molecular docking. Results indicated that n-hexadecanoic acid, DL-proline, 5-oxo-, and <em>cis</em>-vaccenic acid showed high binding affinities to specific therapeutic targets, suggesting strong potential as biotherapeutic agents. This current inquiry concentrates on the therapeutic potential of marine bacteria from mangrove ecosystems as a source of bioactive compounds, positioning <em>Oceanimonas</em> sp. JM-AZM31 and <em>L. fusiformis</em> JM-AZM37 as promising candidates for developing new biotherapeutic treatments.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"23 3","pages":"Article 100538"},"PeriodicalIF":3.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144656295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}