Environmental DNA最新文献

{"title":"Tracking Aquatic Biodiversity With Environmental DNA: A Study in Quebec's Mining Region","authors":"Fidji Sandré,&nbsp;Tuan Anh To,&nbsp;Julie Couillard,&nbsp;Annie-Claude Bélisle,&nbsp;Valérie S. Langlois","doi":"10.1002/edn3.70179","DOIUrl":"https://doi.org/10.1002/edn3.70179","url":null,"abstract":"<p>In Quebec's James Bay region, mining activities pose significant risks to local aquatic biodiversity due to habitat disturbance and potential contaminant release. This study evaluates the efficacy of environmental DNA (eDNA) for detecting and monitoring fish species in areas affected by mining operations, specifically at an active gold mine and a prospective lithium mine. Over two sampling campaigns, eDNA analyses identified the presence of four target fish species, including species of ecological and cultural significance to the Cree communities. The eDNA method proved to be a sensitive and non-invasive tool, capable of detecting species across large aquatic ecosystems and providing insight into species distribution and abundance in relation to environmental changes. Results indicate that certain species, like lake sturgeon or sauger, may be absent or less detectable in mining-impacted areas, potentially due to habitat fragmentation and altered water quality parameters, including low pH and elevated heavy metal concentrations. Our findings support the integration of eDNA as a valuable monitoring tool for assessing biodiversity and establishing species presence baselines in sensitive ecosystems and highlight its potential for community-led environmental management initiatives in Indigenous territories.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"7 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Passive Environmental DNA Sampler for Aquatic Biodiversity Detection Tested in Marine Mesocosms","authors":"Dimitri Medetian,&nbsp;Alexia Delnatte,&nbsp;Julien Cambedouzou,&nbsp;Philippe Clair,&nbsp;David Cornu,&nbsp;Yvan Duhamel,&nbsp;Gilbert Dutto,&nbsp;Mikhael Bechelany,&nbsp;Thibault Geoffroy,&nbsp;Jean-Luc Rolland,&nbsp;Claude Miaud","doi":"10.1002/edn3.70183","DOIUrl":"https://doi.org/10.1002/edn3.70183","url":null,"abstract":"<p>In aquatic ecosystems, environmental DNA (eDNA) can be collected from water samples to produce species inventories. One method for this is passive eDNA sampling, whose development for aquatic biodiversity monitoring is in its early stages. While several materials have been successfully tested for passive eDNA samplers (PEDS), methodological advances are still needed to explore their versatility as a complement to the more common method of active filtration. This study tested for the first time a PEDS using human-crafted material in controlled marine mesocosms (1 m³) containing one species, the European seabass (<i>Dicentrarchus labrax</i>) in different conditions of fish density (1, 5, 10, 100 fish per m³) and exposure times (30 min, 2 h, 8 h, 24 h). We then tested the influence of another source of eDNA on the sampler's performance by introducing another species, the Pacific oyster (<i>Magallana gigas</i>). In addition, we compared the efficacy of the method with active filtration. The PEDS we produced consisted of a small electrospun polyacrylonitrile (PAN) membrane encapsulated in a custom-made 3D-printed frame. Each sampler is low-cost, easy to manipulate, highly replicable, and customizable. A specific quantitative polymerase chain reaction-based assay for the seabass was developed. Results were analyzed with multiscale occupancy modeling and continuous response variable modeling. We found that the PAN-PEDS efficiently collected eDNA in a large volume (1 m³) of renewed water (1 m³/h), with a clear positive effect of high fish density on fish detection. The introduction of oysters did not significantly influence detection. Regarding exposure times, a range of results were obtained that could be attributed to the unreached equilibrium between eDNA shedding and degradation. While active eDNA collection (30 L) outperformed PAN-PEDS, the ongoing development of passive methods can provide new insights in aquatic species monitoring when spatio-temporal eDNA collection is required.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"7 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70183","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145012199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Flower eDNA Metabarcoding to Identify the Effects of Forest Structure and Microclimate on Flower-Visiting Arthropods","authors":"Joan Díaz-Calafat,&nbsp;Donnie Lee Peterson,&nbsp;Pieter De Frenne,&nbsp;Adam Felton,&nbsp;Erik Öckinger,&nbsp;Sara A. O. Cousins,&nbsp;Fabian Roger,&nbsp;Michelle Cleary,&nbsp;Per-Ola Hedwall","doi":"10.1002/edn3.70185","DOIUrl":"https://doi.org/10.1002/edn3.70185","url":null,"abstract":"<p>Recording flower-associated taxa can be challenging in contexts where plant–arthropod interactions are limited, thereby constraining the assessment of their ecological responses. For example, forests typically provide fewer floral resources for pollinators than other ecosystems, such as grasslands, while understory microclimates influence the spatiotemporal dynamics of insect activity, further complicating their detection. In this study, we use environmental DNA (eDNA) to address these challenges and investigate the influence of forest microclimate, density, and tree composition on the diversity of flower-associated arthropods in a Swedish forest. We used two flowering plant species, <i>Fragaria vesca</i> and <i>Trifolium pratense</i>, as sentinel plants, translocating them to a mixed forest across 40 plots spanning a gradient of forest density and broadleaf tree dominance. The metabarcoding of flower eDNA documented a high diversity of arthropods with very specific communities in different forest plots. This high species turnover suggests either short eDNA persistence on flowers or unmeasured ecological factors structuring these communities. We found that forest structure, particularly light availability in broadleaf-dominated open plots, positively influenced species richness of arthropods detectable in flowers, while microclimate had a small impact. These effects varied between plant species, likely due to differences in flower visitor communities. Our study also offers significant methodological insights into using flower eDNA for detecting flower-associated taxa. We also emphasize the need for optimized sampling and DNA extraction processes to enhance the likelihood of successful amplification. We show that the number of flowers pooled in the same DNA extraction positively influences the number of taxa detected. By improving methods in flower eDNA sampling and analysis, future studies can more accurately assess the ecological interactions and conservation needs of forest environments and other ecosystems.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"7 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144934753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining Hydroacoustics and eDNA to Estimate Species-Specific Biomass in a Pelagic Fish Community","authors":"Victoria Elizabeth Kopf,&nbsp;Lee Frank Gordon Gutowsky,&nbsp;Kristyne Wozney,&nbsp;Caleigh Smith,&nbsp;Chris C. Wilson,&nbsp;Derrick T. de Kerckhove","doi":"10.1002/edn3.70145","DOIUrl":"https://doi.org/10.1002/edn3.70145","url":null,"abstract":"<p>Hydroacoustic surveys and eDNA monitoring are rapidly evolving technologies with significant applications for monitoring fish populations. Hydroacoustic technology is capable of enumerating size classes; however, species identification often relies on time-consuming, costly, and lethal supplementary sampling methods. Environmental DNA (eDNA) detection is a nonlethal alternative for ground-truthing hydroacoustic surveys; however, on its own, it does not provide estimates of fish size or stock biomass. We tested the utility of paired hydroacoustic and eDNA surveys by replicating samples over a 12-h period along the depth gradient of pelagic lake habitat where the fish community exhibits diel vertical migration. Generally, we found that (1) the detection and proportion of target species estimated by eDNA was similar to those found in historical gill-netting across depth strata, (2) eDNA-apportioned hydroacoustic data agreed with expected diel patterns in species vertical distributions, and (3) with some exceptions, eDNA-apportioned hydroacoustic estimates of biomass were strongly correlated with expected species biomass. Some species yielded unrealistically high concentrations in the deepest samples, suggesting that benthic accumulation of eDNA can result in inflated biomass estimates near the lake bottom. Combining eDNA and hydroacoustics as complementary noninvasive assessment tools provides a simplified species apportioning protocol for future fish populations and community assessments.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"7 5","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144934754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Importance of Within-Log Sampling Replication in Bark- and Wood-Inhabiting Fungal Metabarcoding Studies","authors":"D. Naranjo-Orrico,&nbsp;J. Purhonen,&nbsp;B. Furneaux,&nbsp;K. Ketola,&nbsp;O. Ovaskainen,&nbsp;N. Abrego","doi":"10.1002/edn3.70181","DOIUrl":"https://doi.org/10.1002/edn3.70181","url":null,"abstract":"<p>Despite the widespread use of metabarcoding approaches in wood-inhabiting fungal studies, there is currently no standardized procedure for sampling deadwood. How to sample and how much to replicate within logs varies among studies, making comparisons difficult. Here, we provide quantitative results about how bark- and wood-inhabiting fungi vary along logs in early decay stages, compared to how they vary between logs. For this, we used two datasets: one representing variation in the sample's location along the logs (tree part; bottom, middle or top) and across tree species (pine or spruce), and another representing small-scale variation (tens of centimeters) in sampling location and variation in sample type (wood or bark). Additionally, we conducted a visual survey of epiphytic lichens to assess the efficiency of bark metabarcoding for surveying epiphytic lichens. Our results show that the diversity of fungi varies greatly between tree species and among the tree parts. Yet, within the tree parts, fungal community composition is relatively homogeneous, with an increasing number of samples only moderately increasing the number of species detected. Strikingly, our results reveal bark samples to be especially species-rich, holding threefold the diversity of the wood beneath it, which represents a subset of the diversity found in bark. This finding suggests that the common practice of excluding bark-inhabiting fungal diversity in studies of saproxylic species in early decay-stage logs overlooks a substantial part of the saproxylic diversity. We found a poor overlap between lichen species identified morphologically and those detected via bark metabarcoding; however, both methods captured consistent patterns in how lichen diversity varied across the logs. We conclude that to gain a representative view of the fungal community composition in early decay-stage deadwood, bark should be included in fungal surveys and that replication within logs should focus on covering the different tree parts.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"7 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70181","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A State-Of-The-Art Review of Aquatic eDNA Sampling Technologies and Instrumentation: Advancements, Challenges, and Future Prospects","authors":"Kevan M. Yamahara,&nbsp;Elizabeth A. Allan,&nbsp;Julie Robidart,&nbsp;William H. Wilson,&nbsp;James M. Birch,&nbsp;Pascal Craw,&nbsp;Ethan Edson,&nbsp;Ivory B. Engstrom,&nbsp;Tatsuhiro Fukuba,&nbsp;Annette F. Govindarajan,&nbsp;Alfredo M. Martins,&nbsp;Kim M. Parsons,&nbsp;Vincent J. Sieben,&nbsp;Austen Thomas,&nbsp;Ian Wilson,&nbsp;Christopher A. Scholin","doi":"10.1002/edn3.70170","DOIUrl":"https://doi.org/10.1002/edn3.70170","url":null,"abstract":"<p>The field of environmental DNA (eDNA) analysis has revolutionized our ability to detect and monitor biodiversity in aquatic and terrestrial ecosystems. However, traditional eDNA sampling methods often present limitations in terms of temporal and spatial coverage, resulting in a loss of resolution associated with infrequent events or those prohibitive to onsite fieldwork. In recent years, the emergence of autonomous eDNA sampling technology has provided researchers with a powerful tool for collecting high-resolution genetic data, overcoming many of the challenges associated with manual sample acquisition. This review focuses exclusively on eDNA technologies designed for the collection and preservation of water samples, to provide a comprehensive overview of the current landscape of aquatic autonomous eDNA sampling technology and instrumentation. A new era of instrument development and capabilities is emerging; the result of knowledge gained through experience with long-tested marine biological observation instrumentation. Lastly, we highlight current research to develop an in situ eDNA analytical capability, as well as explore the challenges and future prospects associated with this rapidly evolving field.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"7 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “A Metadata Checklist and Data Formatting Guidelines to Make eDNA FAIR (Findable, Accessible, Interoperable, and Reusable)”","authors":"","doi":"10.1002/edn3.70184","DOIUrl":"https://doi.org/10.1002/edn3.70184","url":null,"abstract":"<p>Takahashi, M., Frøslev, T.G., Paupério, J., Thalinger, B., Klymus, K., Helbing, C.C., Villacorta-Rath, C., Silliman, K., Thompson, L.R., Jungbluth, S.P., Yong, S.Y., Formel, S., Jenkins, G., Laporte, M., Deagle, B., Rajbhandari, S., Jeppesen, T.S., Bissett, A., Jerde, C., Hahn, E.E., Schriml, L.M., Hunter, C., Newman, P., Woollard, P., Harper, L.R., Dunn, N., West, K., Haderlé, R., Wilkinson, S., Acharya-Patel, N., Lopez, M.L.D., Cochrane, G. and Berry, O. (2025). A Metadata Checklist and Data Formatting Guidelines to Make eDNA FAIR (Findable, Accessible, Interoperable, and Reusable). Environmental DNA, 7: e70100. https://doi.org/10.1002/edn3.70100</p><p>In paragraph 4 of the “Section 5.2”, the text “extending these requirements to additional journals including <i>Molecular Ecology and Evolution</i>.” was incorrect. This should have read “extending these requirements to additional journals including <i>Molecular Ecology</i> and <i>Ecology and Evolution</i>.”</p><p>This has now been fixed in the published article.</p><p>We apologize for this error.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"7 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70184","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monthly eDNA Metabarcoding Data From the Coastal North Sea Area Align With Fish Species-Specific Life Cycle Events","authors":"Marianne Lolivier,&nbsp;Isolde Cornelis,&nbsp;Sara Maes,&nbsp;Jonas Mortelmans,&nbsp;Hans Polet,&nbsp;Sofie Derycke","doi":"10.1002/edn3.70175","DOIUrl":"https://doi.org/10.1002/edn3.70175","url":null,"abstract":"<p>Coastal environments serve as essential nursing, feeding, and spawning grounds for commercially and ecologically important fish species, some of which use nearshore habitats as transitional steps in their ontogenetic migration. Understanding fish communities' spatial and temporal dynamics in coastal habitats is fundamental for sustainable ecosystems and fisheries management. Despite the importance of long-term monitoring to obtain information on fish movements and distribution, fine-scale temporal datasets on fish communities remain scarce due to the intense field work required. In the present study, we explored the use of eDNA 12S metabarcoding of seawater samples to monitor fine-scale temporal and spatial patterns in fish communities. In total, 168 samples were collected across 20 sampling campaigns conducted monthly between August 2021 and August 2023 within the 12 nautical miles of the Belgian part of the North Sea. eDNA patterns revealed no marked temporal or spatial patterns at the community levels due to the ubiquitous presence of the dominant Southern North Sea fish species linked to their non-migratory behavior and use of the coastal Belgian waters as nursing and spawning grounds. However, species-specific temporal patterns reflected their reproductive activity and seasonal migrations. Additionally, fish species spatial distribution was consistent with previous beam trawl and eDNA-based surveys conducted within the Belgian part of the North Sea and was mainly driven by the environmental gradient created by freshwater discharge from the Scheldt estuary. Our findings demonstrate that eDNA metabarcoding is a valuable biomonitoring tool and provides insight into fish distribution, migration, and reproductive activity.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"7 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling Structure of Tropical Estuarine Communities Through eDNA and Implications for Biomonitoring","authors":"Johan Pansu,&nbsp;Christine Chivas,&nbsp;Geoffrey Carlin,&nbsp;Jacob Gruythuysen,&nbsp;Marcelo Merten Cruz,&nbsp;Rachael A. Smith,&nbsp;Fréderique Viard,&nbsp;Stuart L. Simpson,&nbsp;Anthony A. Chariton","doi":"10.1002/edn3.70180","DOIUrl":"https://doi.org/10.1002/edn3.70180","url":null,"abstract":"<p>Tropical estuaries are hyper-diverse ecosystems, hosting essential habitats for freshwater, euryhaline, and marine life. Understanding how biological communities are distributed in these systems has long been a challenge because of their inherent dynamic nature and the diversity of interacting natural pressures and anthropogenic stressors they are subjected to. In addition, most studies focus on a single taxonomic group, hindering a comprehensive understanding of the interactive effects of natural and human-driven environmental variations on the different components of tropical estuarian biodiversity. In this study, we used environmental DNA (eDNA) metabarcoding to examine the structure of multi-taxonomic communities, from diatoms to fish, and their relationships with environmental drivers in three differentially impacted locations facing the Great Barrier Reef in Central Queensland (Australia). We first demonstrated that eDNA signals from sediment and water matrices provide complementary information and that both should be monitored for a more holistic understanding of community trajectories in anthropogenically impacted aquatic environments. We also observed that, independently of the taxonomic group considered, communities were primarily structured by the ecological conditions of the estuary. A within-estuary differentiation along an upstream–downstream gradient was detected but only for small-bodied organisms, which further adds credence to eDNA approaches as an ecologically relevant tool for monitoring fine-scale biodiversity patterns even in profoundly dynamic environments. Finally, the different communities exhibited contrasting response patterns, in terms of diversity, composition, and uniqueness, to the anthropogenic gradient. Hence, our findings emphasize the need for multi-taxonomic assessments, for which eDNA is well suited, to better understand the impacts of multiple stressors on biodiversity and thereby assist decision makers in the protection and management of tropical estuaries.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"7 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70180","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flower-Derived Environmental DNA Reveals Community Diversity, Species Abundances and Ecological Interactions in Bee Pollinators","authors":"Arndt Schmidt,&nbsp;Lukas Schilbach,&nbsp;Arno Schanowski,&nbsp;Michael Erik Grevé,&nbsp;Christian Ulrich Baden,&nbsp;Christian Maus,&nbsp;Henrik Krehenwinkel","doi":"10.1002/edn3.70178","DOIUrl":"https://doi.org/10.1002/edn3.70178","url":null,"abstract":"<p>Flower-derived eDNA holds great promise as a rapid and non-invasive tool for monitoring pollinators and their plant associations. However, pollinators often only briefly interact with a plant and leave little eDNA, making them particularly challenging to detect. In addition, taxonomic biases in eDNA deposition and PCR amplification prevent quantitative analysis of pollinator diversity. These limitations have so far precluded the widespread use of eDNA in pollinator monitoring. Comparing flower-derived eDNA with conventional monitoring in flower strips, we here explore the utility of eDNA to detect community diversity, species abundances, and ecological specificity of plant-associated arthropods. We show that read abundances are a bad predictor of true abundances at the community level. Instead, the occupancy of individual species in replicated flower eDNA samples provides reliable quantitative estimates of pollinator biodiversity and detects their ecological specificity very well. Also, we find that pollinator eDNA can be collected non-invasively, by washing off from flowers in the field. Our work highlights eDNA analysis as a powerful tool for the rapid future monitoring of plant-arthropod interactions and plant-pollinator networks.</p>","PeriodicalId":52828,"journal":{"name":"Environmental DNA","volume":"7 4","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/edn3.70178","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144861746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}