Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology最新文献_第3页

Association of IL-17A (rs2275913) G/A and IL-21 (rs4833837) C/T Gene Polymorphisms in Iraqi patients with Rheumatoid Arthritis 伊拉克类风湿关节炎患者IL-17A (rs2275913) G/A和IL-21 (rs4833837) C/T基因多态性的相关性研究

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology Pub Date : 2023-10-07 DOI: 10.21608/eajbsc.2023.319638

Athraa Al-Shemmary, Nawfal Aldujaili

{"title":"Association of IL-17A (rs2275913) G/A and IL-21 (rs4833837) C/T Gene Polymorphisms in Iraqi patients with Rheumatoid Arthritis","authors":"Athraa Al-Shemmary, Nawfal Aldujaili","doi":"10.21608/eajbsc.2023.319638","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.319638","url":null,"abstract":"cytokines, such as interleukin (17A )and interleukin (21), genetic variation, and environmental variables induce it. The purpose of this study is to examine IL-17A (rs2275913) G/A and IL-21 (rs4833837) C/T gene polymorphism association in Iraqi patients with rheumatoid arthritis. The current study includes 120 subjects classified into two main groups: 60 RA patients and 60 healthy controls. Tetra ARMS-PCR and Allele-specific PCR were used for the detection of IL-17A and IL-21 gene polymorphisms respectively. Genotype and allele frequency results of IL17 rs2275913 G/A and IL-21 rs4833837 C/T gene polymorphisms were analyzed under codominant, dominant, recessive, and additive models. IL17A (rs2275913) G/A under codominant pattern with heterozygous genotype (G/A) and dominant pattern, those of the GA+AA genotypes significantly higher in RA patients with respect to those of the control group (OR:3.3, CI 95%: ( 1.11 to 9.96), P< 0.03) and (OR: 3.35, CI 95%: ( 1.12 to 9.99), P< 0.03) respectively. In addition, this genetic polymorphism doesn’t appear significant variation in other inheritance models. The allele (A) frequency was observed to be higher in RA patients (49%) opposite the allele (G) frequency observed to be higher in control (59%) without significant variation between them, On the other hand, IL-21 rs4833837 C/T genotyping and allele frequencies results revealed no significant variation between the rheumatoid arthritis patients in comparison with the control groups.","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135302076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of Virulence Factor Genes Pvl, nuc, Sea, icaA in Methicillin-Resistant in Staphylococcus aureus In Al Najaf City 纳贾夫市耐甲氧西林金黄色葡萄球菌毒力因子基因Pvl、nuc、Sea、icaA的流行

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology Pub Date : 2023-10-06 DOI: 10.21608/eajbsc.2023.319286

Fatima Nasser, Ebtehal Shubbar

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Role of Integrons 1 and 2 in Proteus spp. and Evaluation of Some Antibiotic's Resistance Genes 整合子1和整合子2在变形杆菌中的作用及部分耐药基因的鉴定

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology Pub Date : 2023-10-05 DOI: 10.21608/eajbsc.2023.319284

Mustafa AL-Fatlawi, Oday Zwain, Zahraa Alquraishi

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Ecofriendly System Based on Mesoporous Silica Nanoparticles Delivery of Chitosan to Control Soft Rot Disease and Maintain Postharvest Quality of Tuber Potato 基于介孔二氧化硅纳米颗粒输送壳聚糖的马铃薯软腐病防治及采后品质维持体系研究

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology Pub Date : 2023-10-01 DOI: 10.21608/eajbsc.2023.318812

Sodaf Maan, Gamar M., Abeer Faiesal, Hoda El-Zehery

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The Association between Epstein Barr virus and Systemic Lupus Erythematosus among Hemodialysis Patients eb病毒与血液透析患者系统性红斑狼疮的关系

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology Pub Date : 2023-09-30 DOI: 10.21608/eajbsc.2023.318717

Zahraa Shaker, Huda AL-Khilkhali

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A New Paper-Based Microfluidic Design (µPAD) for Determination of Total Phenols and Biological Applications Using Smartphone Sensor as a Detector 一种基于纸张的微流体设计(µPAD)用于测定总酚及其生物应用，使用智能手机传感器作为检测器

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology Pub Date : 2023-09-25 DOI: 10.21608/eajbsc.2023.317939

Mahmoud Rashed, Mustafa Hussein

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Identification of Ovarian Cancer Using in Silico-Based Analysis of the Downregulated Expressed miRNAs 用硅基分析下调表达的mirna来鉴定卵巢癌

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology Pub Date : 2023-09-23 DOI: 10.21608/eajbsc.2023.317702

Bushra Mohammed, Sherzad Mustafa, Bayar Zeebaree

{"title":"Identification of Ovarian Cancer Using in Silico-Based Analysis of the Downregulated Expressed miRNAs","authors":"Bushra Mohammed, Sherzad Mustafa, Bayar Zeebaree","doi":"10.21608/eajbsc.2023.317702","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.317702","url":null,"abstract":"levels using small RNAs molecules including microRNAs. These molecules have played a key role in the growth of malignant tumour of ovary including cellular proliferation and metastasis. We carried out a meta-analysis of previously published miRNA expression datasets (two human datasets GSE83693 and GSE119055) and one mouse GSE98391 to identify the downregulated miRNA and its target genes with biological processes and pathways. Meta-analysis of miRNA datasets showed that miR-378a-3p, miR-378a -5p and miR-378c are commonly downregulated miRNAs among the three databases in cancerous samples in comparison to normal samples. A total of 405 common gene targets for miR-378a-3p, -5p and miR-378c were identified using miRWALK. Enrichment analysis revealed that miRNAs target genes were predominantly linked to protein binding as well as in Ras signalling pathways. In addition, multiple hub miRNA target genes in the PPI network provided poor prognosis for the patients with OC including FLT1, its level was closely relevant to ovarian cancer. Overall, these investigations exhibited that the defined miRNAs and their target genes could be exploited as biomarkers to identify ovarian malignancies and achieve an early effective therapy.","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135966771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Estimation of Total Antioxidant for The Patients with Diabetic Nephropathy by Spectrophotometer and Microfluidic Sensor 用分光光度计和微流体传感器测定糖尿病肾病患者总抗氧化水平

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology Pub Date : 2023-09-21 DOI: 10.21608/eajbsc.2023.317544

Abdullah Al-Mousawi, Ahmed Al-Khafagi, Ali Al-Kufaishi

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Evaluate the Levels of Lysophosphatidic Acid and Lipid Profile in Patients with Breast Cancer 评估乳腺癌患者溶血磷脂酸和脂质水平

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology Pub Date : 2023-09-15 DOI: 10.21608/eajbsc.2023.317442

Reham Khaled, Rafah Al-samarrai, Ahmed Abdel Hamid

{"title":"Evaluate the Levels of Lysophosphatidic Acid and Lipid Profile in Patients with Breast Cancer","authors":"Reham Khaled, Rafah Al-samarrai, Ahmed Abdel Hamid","doi":"10.21608/eajbsc.2023.317442","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.317442","url":null,"abstract":"The current study aims to evaluate the levels of lysophosphatidic acid-LPA and lipid profile in sera of women with breast cancer-BC. Ninety serum samples were taken from women; 30 samples were taken from healthy women (control group-C) without a history of BC, and 60 samples were divided into two groups: 30 untreated BC patients as first group-G1, and 30 post-treatment as the second group-G2. The study includes the determination of the level of serum LPA, total cholesterol-TC, triglycerides-TG, high-density lipoprotein-HDL, low-density lipoprotein-LDL and very low-density lipoprotein-VLDL. The results indicate that the value of body mass index-BMI was significantly higher in G1 as compared with G2 and the control group. The level of LPA and HDL was significantly elevated in the patients' group, while the levels of serum TC, TG, LDL, VLDL and TC/HDL ratio were significantly decreased in the patients' groups(G1 and G2) as compared with C. From the results of the current study, we can conclude that LPA plays an important role in the development of BC in new diagnostic cases or after treatment, while the correlation between lipids and breast cancer is obscure and needs more studies.","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"140 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135485645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Carbapenemase-Encoding Genes in MDR Acinetobacter baumannii Isolated from ICUs and Surgical Wards in Minya, Egypt 埃及明亚icu和外科病房多药耐药鲍曼不动杆菌碳青霉烯酶编码基因分析

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology Pub Date : 2023-09-13 DOI: 10.21608/eajbsc.2023.317111

Nehal Koriem, Noha Hassuna, Ahmed Mahmoud, Amani Abd El- moaty, Medhat El Fattah

{"title":"Carbapenemase-Encoding Genes in MDR Acinetobacter baumannii Isolated from ICUs and Surgical Wards in Minya, Egypt","authors":"Nehal Koriem, Noha Hassuna, Ahmed Mahmoud, Amani Abd El- moaty, Medhat El Fattah","doi":"10.21608/eajbsc.2023.317111","DOIUrl":"https://doi.org/10.21608/eajbsc.2023.317111","url":null,"abstract":"are among the last resort classes of antibiotics having the greatest efficacy against severe infections caused by bacteria that are resistant to many other antibiotics. This research was done in order to detecte the frequency of carbapenememase genes in A. baumannii isolated from Surgical Wards and Intensive Care Units (ICUs) in Minia, Egypt by phenotypic and genotypic methods. Methods: 310 patients who were undergoing surgery and receiving care in ICUs participated in the current study between August 2017 and October 2020. The isolates were obtained from a variety of clinical samples, including sputum, bronchial lavage, Pus and wound fluid. All clinical samples have been collected under complete aseptic conditions. A. baumannii isolates were identified by standard microbiological tests. The resistance patterns of the obtained isolates were detected by the disc diffusion method. Production of carbapenem-resistant genes was determined by phenotypic tests the Modified Hodge Test (MHT) and confirmed by the modified carbapenem inactivation method (mCIM). Various carbapenem-resistant genes were detected by PCR. Results :Out of 310 samples, Twenty-Five 25 (8%) isolates of A. baumannii were obtained. Antibiotic and sensitivity tests detect that 16(64%) and 12(48%) of A.baumannii isolates were resistant to meropenem and imipenem respectively. Out of the Carbapenem-resistant A. baumannii (CRAB): 9(56%) were (MHT) positive, and 13 (81%) were (mCIM) positive. Regarding resistance genes, bla OXA-23like and bla OXA-51like were detected in 94% and 100% of CRAB isolates respectively, while bla OXA-24like , bla oxa-48like , bla oxa-58like and bla NDM were not detected in CRAB isolates.","PeriodicalId":52600,"journal":{"name":"Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0