Carbapenemase-Encoding Genes in MDR Acinetobacter baumannii Isolated from ICUs and Surgical Wards in Minya, Egypt

Abstract

are among the last resort classes of antibiotics having the greatest efficacy against severe infections caused by bacteria that are resistant to many other antibiotics. This research was done in order to detecte the frequency of carbapenememase genes in A. baumannii isolated from Surgical Wards and Intensive Care Units (ICUs) in Minia, Egypt by phenotypic and genotypic methods. Methods: 310 patients who were undergoing surgery and receiving care in ICUs participated in the current study between August 2017 and October 2020. The isolates were obtained from a variety of clinical samples, including sputum, bronchial lavage, Pus and wound fluid. All clinical samples have been collected under complete aseptic conditions. A. baumannii isolates were identified by standard microbiological tests. The resistance patterns of the obtained isolates were detected by the disc diffusion method. Production of carbapenem-resistant genes was determined by phenotypic tests the Modified Hodge Test (MHT) and confirmed by the modified carbapenem inactivation method (mCIM). Various carbapenem-resistant genes were detected by PCR. Results :Out of 310 samples, Twenty-Five 25 (8%) isolates of A. baumannii were obtained. Antibiotic and sensitivity tests detect that 16(64%) and 12(48%) of A.baumannii isolates were resistant to meropenem and imipenem respectively. Out of the Carbapenem-resistant A. baumannii (CRAB): 9(56%) were (MHT) positive, and 13 (81%) were (mCIM) positive. Regarding resistance genes, bla OXA-23like and bla OXA-51like were detected in 94% and 100% of CRAB isolates respectively, while bla OXA-24like , bla oxa-48like , bla oxa-58like and bla NDM were not detected in CRAB isolates.