Estimation of Total Antioxidant for The Patients with Diabetic Nephropathy by Spectrophotometer and Microfluidic Sensor

Abstract

This study included a new, simple, fast, and sensitive new microfluidic sensor for the determination of total antioxidant (TAC) in patients with diabetic nephropathy and control then compare results with the conventional method (spectrophotometer technique). This method is dependent on the reduction of Cu 2+ into Cu 1+ by the action of antioxidants that are existing in the sample and chelating factor like neocuproine that produced complex colors. The spectrophotometry method was utilized to measure the absorbance of TAC at 450 nm in patients with diabetic nephropathy and control, also measured of TAC by using a innovative microfluidic system that contain a novel double split ring resonator sensor, after that compare outcomes between both techniques that indicated no significance differences in result of TAC after measured by spectrophotometer and new microfluidic sensor.