Zhongguo Zhongyao Zazhi最新文献

{"title":"[Effects and mechanisms of Abelmoschus manihot-derived extracellular vesicle-like particles in inhibiting podocyte oxeiptosis in diabetic kidney disease by targeted regulation of \"mitochondria-endoplasmic reticulum coupling\"].","authors":"Yu Wang, Yan-Ru Huang, Yi-Gang Wan, Hang Zhao, Shi-Yu Shen, Liu-Yin DU, Hui Wang, Tian-Qing Sang, Qi-Han He, Qi-Jun Fang, Wei Wu, Fee-Lan Chong","doi":"10.19540/j.cnki.cjcmm.20260629.701","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260629.701","url":null,"abstract":"<p><p>Abelmoschus manihot-derived extracellular vesicle-like particles(AM-EVLPs), as a novel natural nano-delivery system, have a precise protective effect on podocytes. This study aims to verify the effects in vivo and in vitro of AM-EVLPs in protecting against podocyte injury using a modified rat model with diabetic kidney disease(DKD) and a high-glucose(HG)-induced podocyte model, and then to explore its therapeutic targets based on the pathological mechanisms of "mitochondria-endoplasmic reticulum coupling" and tumor protein 53(P53)-mediated oxeiptosis. The first, AM-EVLPs were extracted from fresh flower petals of Abelmoschus manihot using ultracentrifugation combined with density gradient centrifugation. The second, in the in vivo study, the modified DKD rat model was established. After successful modeling, the DKD model rats were randomly divided into 4 groups, including the model group, the low-dose AM-EVLPs(L-AM-EVLPs)-treated group, the high-dose AM-EVLPs(H-AM-EVLPs)-treated group, and total flavones of Abelmoschus manihot(TFA)-treated group, receiving corresponding drugs for consecutive 4 weeks. In the in vitro study, mouse podocytes(MPC5 cells) were exposed to HG and subsequently treated with L-AM-EVLPs, H-AM-EVLPs, empagliflozin(EMPA), P53 small interfering RNA(P53-siRNA), or P53-overexpressing lentivirus(P53-OE), respectively. Using the DKD model rats, blood glucose, urinary albumin, liver and kidney function-related indicators were observed. Additionally, glomerular histopathological features, foot process and mitochondrial ultrastructure, the protein expression levels of podocyte-specific structural molecules(podocin and nephrin),renal reactive oxygen species(ROS) levels, as well as the protein expression levels of oxeiptosis-related proteins including P53, Kelch-like ECH-associated protein 1(KEAP1), phosphoglycerate mutase family member 5(PGAM5), and apoptosis-inducing factor/mitochondria-associated 1(AIFM1) were detected, respectively. Furthermore, the differential expression proteomic profile of diabetic kidneys was also analyzed. Using the HG-induced podocyte model, the protein expression levels of podocin and nephrin, the degree of mitochondria-endoplasmic reticulum coupling, the binding intensity of the IP3R1-GRP75-VDAC1 complex, mitochondrial calcium ion(Ca~(2+)) concentration, mitochondrial reactive oxygen species(mtROS) levels, and the protein expression levels of key signaling molecules in the KEAP1/PGAM5/AIFM1 signaling axis were detected. The in vivo results showed that, for the DKD model rats, AM-EVLPs dose-dependently improved urinary albumin, blood urea nitrogen(BUN), and serum creatinine(Scr) levels; reduced glomerular cell proliferation, ECM deposition, and collagen deposition; ameliorated foot process fusion and mitochondrial ultrastructural damage; upregulated the protein expression levels of podocin and nephrin; reduced mtROS levels; and reversed the aberrant protein expression levels of key signaling","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 18","pages":"5117-5130"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Systematic review and quality evaluation of core outcome sets in traditional Chinese medicine(2019-2024)].","authors":"Hui-Zhong Zhu, Yu-Yun Li, Zi-Fan Sang, Bo Pang, Jun-Hua Zhang","doi":"10.19540/j.cnki.cjcmm.20260526.502","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260526.502","url":null,"abstract":"<p><p>A core outcome set(COS) refers to the minimum set of outcomes that should be measured and reported in all clinical studies within a specific field. With the development of the COS for traditional Chinese medicine(COS-TCM), there has been a rapid increase in related publications, highlighting the need for a periodic secondary review. This study systematically reviewed and evaluated the quality of published COS-TCM research by comprehensively searching Chinese and English literature databases as well as specialized databases, using qualitative analysis and quality assessment methods. The objective is to synthesize the current state of research, identify common issues and methodological weaknesses, thereby supporting the high-quality advancement of the COS-TCM field. A total of 39 COS-TCM studies were included. It was found that existing studies generally employ sound methodologies. However, methodological improvements are needed in areas such as patient involvement, the development and publication of study protocols, the generation of a preliminary list of outcomes, and managing attrition in Delphi surveys. While the structural presentation is largely complete, reporting remains insufficient regarding the roles played by different stakeholder groups, detailed content of the consensus process, and the presentation of core outcomes. To improve future development, researchers could focus on both methodological application and reporting quality, and also address aspects such as research topic selection, stakeholder engagement, and the development of TCM-specific outcome and measurement instruments to better reflect the therapeutic characteristics and comparative advantages of TCM.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 18","pages":"5408-5416"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Compound Danshen Cataplasm promoting acclimatization to high-altitude environments].","authors":"Yu-Ting Chen, Hong Niu, Jing-Zhe Wang, Meng-Zhen Li, Jin Guo, Yi-Guang Jin, Li-Na DU","doi":"10.19540/j.cnki.cjcmm.20260608.302","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260608.302","url":null,"abstract":"<p><p>To address acute mountain sickness(AMS) easily induced by low pressure, hypoxia, and intense ultraviolet radiation in high-altitude environments, a novel Compound Danshen Cataplasm(CDC) was developed, and its adhesive properties were evaluated. The hippocampal neuron(HT22) hypoxia model and small-animal hypoxia model were established. The anti-hypoxia and anti-AMS efficacy of CDC was assessed by an open field test, novel object recognition test, routine blood examination, sleep duration measurement, determination of brain and lung wet-to-dry ratios, detection of inflammatory and oxidative stress factors, and pathological section observation. In addition, network pharmacology and molecular docking simulations were employed to explore the underlying mechanism of action. The results show that CDC can significantly increase the survival rate of HT22 cells under hypoxia, reduce cell apoptosis, improve spontaneous exploration ability and cognitive function of hypoxic model mice, prolong sleep duration under hypoxic conditions, promote blood cell recovery, alleviate brain and lung edema, significantly reduce the levels of TNF-α, i NOS, IL-6, and MDA in blood, brain tissue, and lung tissue of mice, increase SOD activity(P&lt;0. 01), and markedly alleviate hypoxia-induced brain and lung edema as well as inflammatory cell infiltration. Network pharmacology results reveal that the binding energies between key active components of CDC and core disease targets are all less than-5 k J·mol~(-1), and that CDC can downregulate the protein expression levels of relevant genes STAT3, JAK1, JAK2, and their phosphorylated forms in the brain tissue of mice. In conclusion, CDC can meet the need for prolonged efficacy under urgent and special conditions, demonstrating significant potential application value.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"4949-4960"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Action characteristics and mechanisms of Panlongqi Tablets in treating fractures based on research strategy integrating Meta-analysis,network pharmacology,and experimental verification].","authors":"Xiao-Xuan Li, Jia-Lin Wang, Wei-Jie Li, Yu-Dong Liu, Qian-Qian Yan, Qing-Yu Zhao, Yan-Qiong Zhang, Ya Lin, Chun-Fang Liu, Na Lin","doi":"10.19540/j.cnki.cjcmm.20260120.701","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260120.701","url":null,"abstract":"<p><p>This study adopted a research strategy and method integrating Meta-analysis, network pharmacology, and experimental verification, aiming to clarify the clinical effect and action characteristics of Panlongqi Tablets in treating fractures and preliminarily reveal the related action mechanism. Firstly, 13 randomized controlled trials(RCTs) of Panlongqi Tablets for treating fractures that met the criteria were retrieved and included. The analysis results show that compared with the control group, Panlongqi Tablets could significantly improve the imaging manifestations and motor function of patients, increase the total effective rate, reduce the swelling score and coagulation indicators, and increase the osteogenic factors, thereby promoting fracture healing. Secondly, the differential gene set of rat fractures at different stages(seven, 14, and 28 days) in the GEO database, namely GSE262132, was collected, and the drug target spectra of Panlongqi Tablets were obtained from databases such as TCMSP. Through the correspondence evaluation for the &quot; disease at different stages and prescription&quot; by using Jaccard and cosine similarity, it was found that the best correspondence was at seven days(early stage). Through the construction of the &quot; differential genes at different fracture stages and candidate targets of Panlongqi Tablets&quot; interaction network for functional enrichment analysis, it was found that the core targets in each stage were enriched in signaling pathways regulating inflammation immunity, pain, blood circulation, and bone metabolism, among which the correlation was the strongest as the phosphatidylinositol-3-kinase(PI3K)-protein kinase B(Akt) signaling pathway. The mechanism of the above candidate signaling molecules was verified by using a rat model of tibial transverse fracture. Western blot was used to detect the expression levels of PI3K, Akt, phosphorylated protein kinase B(p-Akt), and vascular endothelial growth factor A(VEGFA), and ELISA was used to detect the expression levels of transforming growth factor β(TGF-β), bone morphogenetic protein-2(BMP-2), bone alkaline phosphatase(BALP), type I procollagen amino-terminal propeptide(PINP), and osteocalcin(OCN). The results show that Panlongqi Tablets could significantly increase the protein expression levels of PI3K, p-Akt, Akt, and VEGFA in the healing tissue and could significantly elevate the contents of TGF-β, BMP-2, BALP, PINP, and OCN in the serum, indicating that the mechanism of Panlongqi Tablets promoting fracture healing may be related to the activation of the PI3K-Akt signaling pathway. The research results are helpful to clarify the efficacy connotation of the &quot; Qi yao&quot; and guide the rational clinical medication of Panlongqi Tablets.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"4827-4837"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Action mechanism of Fangji Huangqi Decoction in treating knee osteoarthritis via regulating AMPK/mTOR pathway and activating autophagy based on network pharmacology and animal experiments].","authors":"Hou-Yuan Wang, Jia-Cong Xiao, Xing-Zhu Yao, Shou-Jia Shao, Pai-An He, Nan Yang, Zi-Wei Jiang","doi":"10.19540/j.cnki.cjcmm.20260523.502","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260523.502","url":null,"abstract":"<p><p>Combining network pharmacology and animal experiments,this study explored the mechanism of Fangji Huangqi Decoction in intervening knee osteoarthritis(KOA).Potential targets of Fangji Huangqi Decoction for the treatment of KOA were obtained through multiple databases,such as traditional Chinese medicine system pharmacology(TCMSP) and Gene Cards.A proteinprotein interaction(PPI) network was constructed,and GO function and KEGG pathway enrichment analysis was performed by using the bioinformatics resource database,namely the Database for Annotation,Visualization,and Integrated Discovery(DAVID).Molecular docking was conducted between key components and core targets.Rats were divided into a control group,a model group,and Fangji Huangqi Decoction groups with low and high doses.The KOA model was established by anterior cruciate ligament transection(ACLT).Cartilage and synovium were evaluated by hematoxylin-eosin(HE) staining and safranin O-fast green(SFG)staining.The levels of interleukin(IL)-1β,tumor necrosis factor-α(TNF-α),and IL-6 were detected by enzyme-linked immunosorbent assay(ELISA).The expressions of microtubule-associated protein 1 light chain 3β(LC3B),phosphorylated AMPactivated protein kinase(p-AMPK),and phosphorylated mammalian target of rapamycin(p-mTOR) were detected by immunohistochemistry(IHC).The expressions of collagen type Ⅱ alpha 1 chain(COL2A1),aggrecan(ACAN),matrix metalloproteinase 13(MMP13),p-AMPK,p-mTOR,autophagy receptor SQSTM1(p62),and LC3-Ⅱ/Ⅰ were detected by Western blot.Network pharmacology analysis identified 180 potential targets of Fangji Huangqi Decoction for the treatment of KOA,which were mainly enriched in AMPK and autophagy-related pathways.Animal experiments show that compared with model group,Lequesne MG scores,OARSI scores,and Mankin scores were significantly reduced in Fangji Huangqi Decoction group(P&lt;0.01,P&lt;0.001).ELISA results show that the levels of IL-1β,TNF-α,and IL-6 were significantly decreased in Fangji Huangqi Decoction group(P&lt;0.001).IHC results show that the expressions of LC3B and p-AMPK are up-regulated,while the expression of p-mTOR was downregulated in Fangji Huangqi Decoction group(P&lt;0.001).Western blot results show that Fangji Huangqi Decoction increases the expressions of COL2A1,ACAN,p-AMPK,and LC3-Ⅱ/Ⅰ,and decreases the levels of p-mTOR,MMP13,and p62(P&lt;0.001),with a more obvious effect in the high-dose group.In conclusion,Fangji Huangqi Decoction may improve KOA by activating the AMPK/mTOR signaling pathway to regulate autophagy,alleviate inflammatory response,and promote cartilage matrix restoration.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"5062-5072"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149186097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Evidence mapping analysis of clinical research on Chinese patent medicines for treating carotid atherosclerosis].","authors":"Ying-Tian Yang, Xin-Zheng Hou, Shi-Han Wang","doi":"10.19540/j.cnki.cjcmm.20260523.501","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260523.501","url":null,"abstract":"<p><p>This study aims to systematically map and visualize the landscape of clinical research evidence on Chinese patent medicines for treating carotid atherosclerosis(CAS), evaluating the current status, strengths, and limitations of research in this field to provide guidance for future studies. A systematic search was conducted across eight Chinese and English databases from their inception to November 14, 2025. The results were presented using a combination of figures, tables, and textual descriptions. A total of 343 publications were included, comprising 331 randomized controlled trials( RCTs), 8 systematic reviews/Meta-analyses, 1 network Meta-analysis, and 3 expert consensus statements. The publication trends showed an initial rise followed by a decline. The studies involved 68 distinct Chinese patent medicines, with Tongxinluo Capsules, Naoxintong Capsules, and Ginkgo biloba preparations being the most widely used. Most studies had treatment durations of 12 to 24 weeks and sample sizes ranging from 51 to 100 cases. The outcome measures encompassed 19 domains, including plaque parameters, lipid metabolism, safety evaluation, inflammatory factors, and clinical efficacy. The identified limitations included a lack of clear distinction between primary and secondary outcomes, low standardization of outcome measures, insufficient reflection of TCM advantages, absence of clinical endpoints and quality of life assessments, and a lack of health economics indicators. In terms of methodological quality assessment, the RCT exhibited significant deficiencies in randomization, allocation concealment, blinding implementation, and missing data processing, indicating a high risk of bias. The quality of systematic reviews/Meta-analyses was generally low, while expert consensus statements lacked rigor and applicability in their development. Future efforts should focus on conducting multicenter, large-sample, high-quality clinical trials, refining the standardization of TCM syndrome patterns, establishing core outcome indicator sets, and enhancing the overall research quality. This will fully realize the potential of Chinese patent medicines in treating CAS.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"5090-5102"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Identification and quality evaluation of Cistanche deserticola from different production areas based on DNA barcoding and UPLC technology].","authors":"Li Qiu, Wen-Ting Wang, Li Xiang, Ying Li, Bao-Lin Guo, Chao Sun","doi":"10.19540/j.cnki.cjcmm.20260428.406","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260428.406","url":null,"abstract":"<p><p>Cistanche deserticola(Orobanchaceae) has both medicinal and dietary properties and is cultivated on a large scale in Inner Mongolia, Ningxia, Gansu, Qinghai, and Xinjiang. In this study, 37 batches of cultivated C. deserticola samples were collected from five production areas. All samples were identified and differentiated based on the internal transcribed spacer 2(ITS2) sequence. An ultra-performance liquid chromatography( UPLC) method was established to simultaneously determine seven phenylethanoid glycosides, one iridoid glycoside, and two phenolic acids. This method was subsequently applied to the quality analysis and fingerprint comparison of the samples. The results of DNA barcoding identification showed that all 37 samples were authenticated as C. deserticola by the ITS2 sequence, and two single-nucleotide polymorphism(SNP) loci were identified for the first time, which may serve as potential molecular markers to distinguish samples from Inner Mongolia from those originating in other production areas, reflecting the genetic characteristics of the geo-authentic medicinal materials. The results of UPLC determination showed that the content of seven phenylethanoid glycosides in the 37 batches of cultivated C. deserticola was as follows: echinacoside, 0. 31-72. 56 mg·g~(-1); cistanoside A, 0-5. 55 mg·g~(-1); tubuloside A, 0-3. 74 mg·g~(-1); acteoside, 0-45. 34 mg·g~(-1); isoacteoside, 0. 07-5. 60 mg·g~(-1); 2'-acetylacteoside, 0-73. 89 mg·g~(-1); and tubuloside B, 0-11. 82 mg·g~(-1). The content of the iridoid glycoside, geniposidic acid, ranged from 0. 04 to 2. 72 mg·g~(-1), and the content of two phenolic acids, chlorogenic acid and caffeic acid, ranged from 0. 17 to 3. 29 mg·g~(-1) and 0. 28 to 1. 93 mg·g~(-1), respectively. The chemical composition of samples from different batches fluctuated significantly and did not show a consistent correlation with the production area. Moreover, the total content of echinacoside and acteoside in one batch from each of Inner Mongolia, Gansu and Qinghai was lower than the standard specified in the Chinese Pharmacopoeia. The postharvest processing methods might be one of the reasons for this phenomenon. The standardization of production procedures for C. deserticola is crucial for achieving stable and controllable quality for both medicinal and dietary use. This study provides guidance for the formulation of relevant quality standards and promotes the development of the C. deserticola comprehensive health industry.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"4926-4936"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[TCM pathogenesis of adverse reactions to molecularly targeted anti-tumor drugs in light of collateral disease theory].","authors":"Ling-Ling Sun, Yin-Shuang Liu, Jun-Jie Huang, Duo Li, Yi-Ying Lyu, Zi-Hao Zhao, Jia-Yi Li, Li-Zhu Lin","doi":"10.19540/j.cnki.cjcmm.20260514.502","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260514.502","url":null,"abstract":"<p><p>Toxic and adverse reactions associated with molecularly targeted anti-tumor drugs are highly prevalent and seriously affect the patients' quality of life. Drawing upon TCM meridian-collateral theory, this paper elucidated the essentials of TCM pathogenesis of toxic and adverse reactions associated with molecularly targeted anti-tumor drugs. It postulated a core pathological theory defined as &quot; drug pathogens invade the meridians, resulting in disharmony of Qi and blood&quot;. The toxic and adverse reactions of molecularly targeted anti-tumor drugs are not isolated or disordered local injuries but display a well-defined and intrinsically interconnected pattern. Their dynamic progression follows a sequential transmission pattern of &quot; skin and pores-collaterals-meridians-Zang-fu organs &quot;. Pathologically, this manifests as an evolutionary process of wind-heat accumulation in the skin, heat-toxin congestion in the collaterals, Qi stagnation and blood stasis in the meridians, and imbalance of Yin and Yang, which is highly consistent with the pathogen transmission theory described in Miraculous Pivot: Treatise on Contralateral Puncture(Lingshu: Miuci Lun). Toxic and adverse reactions of different classes of molecularly targeted anti-tumor drugs also exhibit distinct affinity for meridians and collaterals. For example, anti-angiogenic drugs primarily affect the Shaoyin meridian system and are prone to inducing hand-foot syndrome, hypertension, and hypothyroidism. Epidermal growth factor receptor tyrosine kinase inhibitors predominantly involve the Yangming and Taiyang meridian systems, commonly leading to cutaneous rash, diarrhea, and interstitial pneumonia. Human epidermal growth factor receptor-2 inhibitors preferentially affect the heart and spleen meridian systems, with cardiac toxicity and anemia being the most frequently observed manifestations. Meridian-collateral theory offers a novel perspective for understanding the TCM pathogenesis of toxic and adverse reactions associated with molecularly targeted anti-tumor drugs, providing a theoretical basis for the clinical implementation of early intervention, meridian-based syndrome differentiation, and stage-specific treatment.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"5110-5116"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on structural characterization and pharmacological mechanisms of supramolecular aggregates in traditional Chinese medicine decoctions].","authors":"Shuang Liu, Hong-Jing Dong, Chuan-Zhi Kang, Yu-Zhang Mi, Xiao Wang","doi":"10.19540/j.cnki.cjcmm.20260603.302","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260603.302","url":null,"abstract":"<p><p>The investigations on pharmacodynamic material basis of traditional Chinese medicine have focused primarily on free compounds, while overlooking the complex interactions among active constituents. In recent years, it has been revealed that active constituents can self-assemble into supramolecular aggregates through non-covalent interactions during the decoction process, providing a new perspective for elucidating the pharmacodynamic material basis of traditional Chinese medicine. This study systematically reviewed the separation and purification methods and structural characterization techniques for supramolecular aggregates, explored their self-assembly mechanisms in depth, and summarized the pharmacological mechanisms. Finally, it examined the limitations of existing studies in terms of separation technologies, self-assembly mechanisms and in vivo metabolism, and proposed future research directions,with the aim of providing a reference for further in-depth research, development and utilization of supramolecular aggregates in traditional Chinese medicine.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"4886-4894"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanism of Danhong Injection in regulating endometrial breakdown during menstruation via HIF1A/CXCR4 pathway].","authors":"Bei-Bei Wu, Xiang Liu, Hong-Hong DU, Nan Liu, Tong-Tong Li","doi":"10.19540/j.cnki.cjcmm.20260603.801","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260603.801","url":null,"abstract":"<p><p>This paper aims to investigate the effects of Danhong Injection on endometrial breakdown in a menstrual-like mouse model and its underlying mechanisms.In this study,a menstrual-like mouse model was established,and uterine tissue morphology was observed at different time points after progesterone(P4) withdrawal.The menstrual-like mice were randomly divided into a sham group,a model group,low-(0.6 mL·kg~(-1)) and high-(1.2 mL·kg~(-1)) dose Danhong Injection groups,and a high-dose Danhong Injection plus hypoxia-inducible factor-lα(HIF1A) inhibitor(PX-478,40 mg·kg~(-1)) group.The results show that 24 hours after P4withdrawal,compared with those in the sham group,the mice in the model group exhibit decreased serum P4 and estradiol(E_2)levels,increased follicle-stimulating hormone(FSH) and luteinizing hormone(LH) levels,endometrial breakdown,and significantly increased apoptosis rate.The gene and protein expression of inflammatory cytokines,such as tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-10(IL-10),and cluster of differentiation 45(CD45) were increased.The gene and protein expression of apoptosis-related proteins,such as activated cleaved caspase-3,HIF1A,and C-X-C chemokine receptor 4(CXCR4),were also increased.The B-cell lymphoma-2(Bcl-2)/Bcl-2-associated X protein(Bax) decreased.After Danhong Injection intervention,especially in the high-dose Danhong Injection group,serum P4 levels were increased,while E_2,FSH,and LH levels were decreased.Endometrial breakdown and apoptosis were alleviated;inflammatory cytokines and cleaved caspase-3 expression were downregulated;IL-10 levels,Bcl-2/Bax,and gene and protein expression of HIF1A and CXCR4 were unregulated.By 48 hours after P4 withdrawal,partial endometrial repair occurred in the mice from the model group,with increased protein expression of α-smooth muscle actin(α-SMA) and matrix metalloproteinase-9(MMP-9),while mRNA expression of HIF1A and CXCR4 showed no significant changes.Although Danhong Injection with different doses did not significantly alter serum hormone levels at this time point,it accelerated endometrial repair and reduced α-SMA and MMP-9 protein expression.The HIF1A inhibitor PX-478 reversed its positive effects during the repair phase.In summary,Danhong Injection may delay P4 withdrawal and improve the hormonal environment,thereby alleviating endometrial breakdown in menstrual-like mice.In the local endometrium,its mechanism of action may be related to activating the HIF1A/CXCR4 signaling pathway,inhibiting excessive stromal cell apoptosis,alleviating inflammatory responses,and promoting tissue homeostasis restoration.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"4972-4982"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}