Zhongguo Zhongyao Zazhi最新文献

{"title":"[Exploration in molecular mechanism of icariin for ameliorating postmenopausal osteoporosis based on estrogen receptor α-mediated regulation of mitophagy in osteoblasts].","authors":"Ning Wang, Nan Cong, Xuan Liu, Si Chen, Jiao-Jiao Yang, Nian Liu, Yu-Xi Dong, Pi-Wen Zhao","doi":"10.19540/j.cnki.cjcmm.20251113.707","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20251113.707","url":null,"abstract":"<p><p>This study investigated the molecular mechanism by which icariin regulates mitophagy via the estrogen receptor α(ERα)-mediated silencing of the regulatory protein 1(SIRT1)/forkhead box protein O3a(FOXO3a) pathway and its downstream PTEN-induced kinase 1(PINK1)/Parkin signaling axis, thereby influencing bone metabolism and osteoblast differentiation to ameliorate postmenopausal osteoporosis(PMOP). Network pharmacology and molecular docking were initially employed to identify key targets of icariin and potential signaling pathways related to PMOP, followed by validation of icariin's binding affinity to these targets. For in vivo experiments, 36 female C57BL/6J mice were randomly divided into six groups: sham-operation, model, estradiol(E_2)-treated, and low-, medium-and high-dose icariin-treated groups, respectively. In the sham operation group, some fat pads around the ovaries were removed from the mice, while the PMOP models were established through castration surgery in other groups. The drug administration groups were respectively given estradiol or icariin for continuous intervention for 8 weeks. Estrus cycle changes were monitored, while serum hormone and bone metabolism levels were measured by ELISA. Meanwhile, the femoral microstructure was evaluated using Micro-CT and HE staining, and bone anabolism was assessed by Western blot, Masson staining, and Goldner staining, respectively. In addition, mitophagy and expression of related proteins were examined by Western blot and transmission electron microscopy, while SIRT1/FOXO3a pathway proteins were analyzed by Western blot. For in vitro experiments, MC3T3-E1 cells were divided into control(osteogenic induction) and icariin-treated groups(low, medium, and high doses), with additional ERα antagonist and SIRT1 inhibitor interventions. Osteogenic differentiation and extracellular matrix mineralization were evaluated using ALP staining, alizarin red staining, and Western blot. Mitophagy and expression of related proteins were examined by Western blot and immunofluorescence detection, while the expression of proteins related to SIRT1/FOXO3a pathway was detected by Western blot. The results of network pharmacology analysis showed that SIRT1/FOXO3a was identified as a critical pathway to regulate PMOP, with icariin exhibiting high binding affinity to ERα. The results of in vivo experiments showed that compared to the sham operation group, the model group exhibited disrupted estrous cycles along with significantly decreased serum E_2 and procollagen type Ⅰ N-terminal propeptide(P1NP) levels, while follicle-stimulating hormone(FSH), luteinizing hormone(LH), and C-terminal telopeptide of type Ⅰ collagen(CTX-1) levels were markedly elevated, indicating successful establishment of the PMOP model. Following icariin intervention, the treatment group showed significantly increased serum P1NP levels and decreased CTX-1 levels compared to the model group. Meanwhile, icariin improved femoral microstructure","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 4","pages":"1016-1030"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147437253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Structural modification of polysaccharides from TCM and their application in delivery systems].","authors":"Ying Zeng, Jin-Ling Duan, Zhi-Yu Guan, Zi-Xia Zhang, Xin-Yao Chen, Zhe Li, Jing Liu, Wen-Ting Wu, Peng-Fei Yue, Wei-Feng Zhu","doi":"10.19540/j.cnki.cjcmm.20251105.301","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20251105.301","url":null,"abstract":"<p><p>The biological activity of polysaccharides from TCM is closely related to their structure. Through structural modifications, such as grafting modification and crosslinking reactions, their physicochemical properties can be significantly improved. Modified polysaccharides from TCM can form nanocarriers through self-assembly, constructing intelligent drug delivery systems and self-healing hydrogels. By esterification, etherification to graft hydrophobic small molecules, radical graft copolymerization, sulfonation reaction to graft ionic polymers, etc., the hydrophilic-hydrophobic balance of polysaccharides can be adjusted, improving their drug loading efficiency and targeted delivery ability. By synergizing crosslinking reactions such as Schiff base reaction, ionic crosslinking, and covalent crosslinking, hydrogels with excellent properties can be constructed. After structural modification, polysaccharides from TCM can achieve efficient encapsulation of hydrophobic active ingredients, construct intelligent drug delivery systems, develop self-healing hydrogels, and build tissue scaffolds. Although they have advantages such as high plasticity and good biocompatibility as delivery systems, there are also potential issues such as stability, immune response, and quality control that need further research and resolution.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 4","pages":"926-935"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147437578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Angelica sinensis polysaccharide-chitosan nanosystem synergizes with radiotherapy to enhance anti-tumor immunity and suppress tumor growth].","authors":"Hao-Jie Wang, Xing-Tai Yu, Zhi-Jun Chen, Tian-Xin Wu, Zhe-Ming Hu, Jia-Yi Fan, Wen-Yi Wang, Yang Lu","doi":"10.19540/j.cnki.cjcmm.20251024.301","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20251024.301","url":null,"abstract":"<p><p>To enhance the immunomodulatory effect of Angelica sinensis polysaccharide(ASP) on the tumor microenvironment, this study prepared ASP-chitosan nanoparticles(ASP-NPs) using a molecular self-assembly technique. The preparation process was optimized through single-factor investigation. The results from dynamic light scattering and transmission electron microscopy showed that ASP-NPs had a particle size of(313.1±23.3) nm, with uniform distribution and regular morphology. In the 4T1 breast cancer mouse model, ASP-NPs combined with radiotherapy(RT) significantly inhibited tumor growth through two administration routes(intratumoral injection and intravenous injection): the tumor inhibition rate reached 69% in the intratumoral injection group and 47% in the intravenous injection group, both significantly higher than that of the RT group(P&lt;0.05). Serum cytokine assays showed that interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α) were significantly elevated under both administration methods(P&lt;0.05). Tumor hematoxylin-eosin(HE) staining revealed that the combined treatment group had significantly expanded necrotic areas in tumor tissues and obvious inflammatory cell infiltration. Immunohistochemical and TUNEL assays showed that ASP-NPs could inhibit tumor cell proliferation and promote apoptosis. Flow cytometry further compared and analyzed dendritic cells(DCs) in tumor-draining lymph nodes and effector T cell subsets in tumor tissues, showing that both intratumoral and intravenous injections increased mature DCs in lymph nodes by over 11.2%. The infiltration rates of CD4~+ T cells and CD8~+ T cells in tumor tissues were significantly increased by 1.6-fold and 2.5-fold respectively compared with the simple RT group(P&lt;0.05). In conclusion, this study reveals the immune activation effect of ASP-NPs combined with radiotherapy, achieving better anti-tumor efficacy, and providing important experimental evidence for tumor immune combination therapy strategies based on traditional Chinese medicine polysaccharides.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 4","pages":"952-961"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147437696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Antidepressant active substance basis of ethanol extract from Nardostachyos Radix et Rhizoma based on spectrum-effect relationships and blood-entering component verification].","authors":"Miao-Miao Yang, Le-le Lei, Xiao-Bin Pu, Zi-Mu Yang, Zhe Pang, Wen-Jing Li, Zhi-Jia Cui, Jing Shao","doi":"10.19540/j.cnki.cjcmm.20251110.202","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20251110.202","url":null,"abstract":"&lt;p&gt;&lt;p&gt;The antidepressant active substance basis of the ethanol extract from Nardostachyos Radix et Rhizoma was analyzed and interpreted by establishing the high-performance liquid chromatography(HPLC) feature spectra and the spectrum-effect relationship of cell damage protection, and combining the analysis and screening of blood-entering components. HPLC was used to establish feature spectra for 18 batches of Nardostachyos Radix et Rhizoma from different origins. Principal component analysis(PCA) and cluster analysis were used to evaluate and analyze the 18 batches of Nardostachyos Radix et Rhizoma. The cytoprotective effect of 95% ethanol extract of Nardostachyos Radix et Rhizoma was evaluated by using a corticosterone-induced PC12 cell damage model. Gray correlation analysis(GCA), partial least squares regression(PLSR) analysis, and Pearson correlation analysis were used to screen for key active components in Nardostachyos Radix et Rhizoma that protect PC12 cells from damage. Blood-entering components were identified by ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry(UHPLC-MS/MS). The results showed that the similarity of the feature spectra of the 18 batches of Nardostachyos Radix et Rhizoma samples established was all &gt; 0.8, with 36 common peaks calibrated and 12 chemical components identified. The results of cluster analysis and PCA were consistent. The 18 batches of Nardostachyos Radix et Rhizoma samples were divided into three categories. A total of 43 prototype components and one metabolite, mainly flavonoids, terpenoids, phenylpropanoids, alkaloids, fatty acids, and other compounds, were detected in the blood-entering components. The results of the gray correlation analysis showed that the correlation of the 36 common peaks in the Nardostachyos Radix et Rhizoma feature spectrum was all &gt; 0.8. PLSR analysis and Pearson correlation analysis preliminarily identified components such as cryptotanshinone, paeonol, aristolone, nardosinone, and luteolin as the key active substances responsible for improving PC12 cell damage in the ethanol extract of Nardostachyos Radix et Rhizoma. The active components identified through spectrum-effect relationship analysis, including cryptotanshinone, paeonol, nardosinone, luteolin, alpha-asarone, and alpha-cyperone, were all verified in the identification of blood-entering prototype components. The study of the spectrum-effect relationship analysis clarified that the improvement of PC12 cell damage by the 95% ethanol extract of Nardostachyos Radix et Rhizoma was the result of the synergistic action of multiple components. Furthermore, through the verification of blood-entering components, the 11 compounds screened out can serve as the active ingredient group responsible for the antidepressant activity of Nardostachyos Radix et Rhizoma. This provides a research foundation and direction for elucidating the substance basis and mechanism of action of the antidepressant activ","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 4","pages":"1001-1015"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147437707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Two new acyclic terpenoids from Croci Stigma].","authors":"Si-Bo Ji, Ting-Ting Li, Jun-Shi Tao, Bai-Xiang Cai, Ju-Tao Wang, Feng-Ling Wang, Yang Yu","doi":"10.19540/j.cnki.cjcmm.20251105.201","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20251105.201","url":null,"abstract":"<p><p>This study isolated chemical constituents from the ethyl acetate fraction of the methanol-dichloromethane extract from Croci Stigma and conducted their structural elucidation by a combination of chromatographic techniques, including silica gel column chromatography, reversed-phase ODS column chromatography, Sephadex LH-20 gel chromatography, and preparative high-performance liquid chromatography(HPLC), alongside spectroscopic methods such as nuclear magnetic resonance(NMR), high-resolution mass spectrometry(HR-MS), and circular dichroism(CD). Five compounds were isolated and identified as crosatin A(1), crosatin B(2), crosatin C(3), crosatin D(4), and(2E,4E)-2-methyl-6-oxo-hepta-2,4-dienoic acid(5). Among them, compounds 1 and 2 are novel compounds, while compounds 3 and 4 were isolated for the first time as new natural products from natural sources. The inhibitory effects of compounds 1-5 on melanogenesis in B16F10 melanoma cells were evaluated in vitro. The results demonstrated that at a concentration of 50 μmol·L~(-1), compounds 1 and 5 significantly reduced α-MSH-induced melanin content in B16F10 cells in a concentration-dependent manner, which suggested their potential as melanogenesis inhibitors and promising skin-whitening agents.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 3","pages":"687-692"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147437670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analgesic mechanism of Entadae Semen based on its in vivo material basis and regulation of SRC-mediated microglial polarization in a rat model of chronic compression of dorsal root ganglion].","authors":"Sen-Sen Li, Han-Xue Wu, Yuan-Bin Li, Li-Min Zhao, Xiao-Hui Su, Hui Xiong, Na Lin, Chao Wang","doi":"10.19540/j.cnki.cjcmm.20251113.401","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20251113.401","url":null,"abstract":"<p><p>The ethnic medicine Entadae Semen shows good therapeutic effects on neuropathic pain(NP) such as chronic low back pain caused by lumbar disc herniation(LDH), yet its in vivo metabolites under pathological conditions and analgesic mechanisms remain unclear. In this study, a chronic compression of the dorsal root ganglion(CCD) model was established to simulate clinical LDH, and behavioral testing, safety evaluation, in vivo material-basis analysis, network pharmacology, and molecular biology were employed to investigate the analgesic mechanisms of the 70% ethanol extract of Entadae Semen(KB). Pharmacodynamic studies showed that KB dose-dependently increased mechanical pain thresholds and thermal radiation pain thresholds in CCD rats, alleviated spontaneous pain, and improved paw print parameters, while exerting no significant effects on basal pain thresholds or motor coordination in normal animals, and showing no obvious toxic or side effects. RESULTS:: from hot plate, tail-flick, and formalin tests suggested that the analgesic effects of KB occurred at the spinal and supraspinal levels and were associated with inhibition of central neuroinflammation. UHPLC-Q-Orbitrap-HRMS identified nine KB-derived components entering the spinal cord, including cuchiloside, curdione, eclalbasaponin V, muscone, 3-O-β-D-galactopyranosyl-(1→2)-β-D-glucuronopyranosyl-gypsogenin-28-O-β-D-xylopyranosyl(1→4)-[β-D-6-O-acetylglucopyranosyl(1→3)]-α-L-rhamnopyranosyl(1→2)-β-D-fucopyranoside, isoalantolactone, N-oxynarcotine, sweroside, and violutoside. Network analysis showed that six core pharmacological targets, i.e., non-receptor tyrosine kinase(SRC), phosphatidylinositol 3-kinase(PI3K), protein kinase B(AKT)1, Kirsten rat sarcoma viral oncogene homolog(KRAS), RAF proto-oncogene serine/threonine-protein kinase 1(RAF1), and extracellular signal-regulated kinase(ERK), were densely enriched in the chemokine signaling pathway and exhibited stable binding to the spinal cord-distributed components. Further experiments using ELISA, Western blot, immunofluorescence, pharmacological inhibitors, and behavioral testing revealed that the analgesic mechanisms of KB may be associated with inhibition of SRC-regulated MAPK and PI3K/AKT signaling axes, thereby suppressing spinal microglial polarization and neuroinflammation. These findings provide a scientific basis for the clinical application and rational use of KB in the treatment of chronic low back pain.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 3","pages":"811-826"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147437813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Shenzao Jiannao Oral Liquid treats Alzheimer's disease by regulating gut microbiota].","authors":"Ye-Xin Zhang, Yi-Jun Shi, Tong Li, Jia-Ming Bai, Yu-Feng Shen, Si-Yu Shan, Hong-Yan Li, Hong-He Xiao","doi":"10.19540/j.cnki.cjcmm.20250725.704","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20250725.704","url":null,"abstract":"<p><p>This study investigates the therapeutic effect and mechanism of Shenzao Jiannao Oral Liquid(SZJN) on Alzheimer's disease(AD) from gut microbiota. APP/PS1 double transgenic mice were used to establish the AD model and then allocated into the following groups: model(normal saline of equal volume), positive drug(donepezil hydrochloride, 0.65 mg·kg~(-1)), and low-, medium-, and high-dose(0.3, 1.5, and 7.5 g·kg~(-1), respectively) SZJN. Meanwhile, C57BL/6J mice of the same brood were selected as the blank group(normal saline of equal volume). All groups were treated by gavage for 8 weeks. The Morris water maze was used to evaluate the spatial exploration, learning, and memory abilities. The nesting test was conducted to assess the daily living abilities. Hematoxylin-eosin(HE) staining was employed to examine histopathological damage in the intestinal tissue. AB-PAS staining was performed to reveal the mucus barrier damage in the intestinal tissue. Western blot was conducted to measure the protein levels of zonula occludens-1(ZO-1) and Occludin. Immunofluorescence(IF) was used to analyze the expression of ZO-1, Occludin, and the macrophage marker F4/80. The diversity, species abundance, and correlations of gut microbiota were analyzed by 16S rDNA sequencing. Animal experiments demonstrated that compared with the model group, the high-dose SZJN group exhibited shortened escape latency, increased platform crossings and time spent in the target quadrant, and improved nesting scores. The high-dose SZJN groups showed alleviated intestinal mucosal contraction, improved cellular morphology, reduced intestinal tissue damage, increased mucin content, and increased goblet cells. Additionally, high-dose SZJN groups exhibited upregulated expression of ZO-1 and Occludin in the intestinal tissue, along with reduced F4/80 protein expression. Gut microbiota analysis revealed increased species diversity and richness in the high-dose SZJN groups, with microbiota structures resembling that in the blank group. These results confirm that high-dose SZJN improves the learning and memory abilities, mitigates intestinal pathological damage, and protects the intestinal tissue of AD mice by regulating the gut microbiota.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 3","pages":"791-798"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147437729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of Viola tianshanica extract on innate immune response in mice infected with influenza A virus based on RIG-Ⅰ/NLRP3 signaling pathway].","authors":"Yan Liu, Fu-Xiang Luo, Xue Wang","doi":"10.19540/j.cnki.cjcmm.20251021.801","DOIUrl":"10.19540/j.cnki.cjcmm.20251021.801","url":null,"abstract":"<p><p>This study aims to investigate the effect of Viola tianshanica Maxim. extract(VTME) on the innate immune response in mice infected with influenza A virus. Mice were randomly divided into a control group, a virus-infected group, an oseltamivir group, and low-, medium-, and high-dose VTME groups, with 10 mice in each group. The model of influenza pneumonia in mice was established via nasal inhalation of influenza A virus(H1N1/PR8), and intragastric administration began on the day of infection, once a day for 4 days, with the lung tissues collected 24 h later. The pathological changes in lung tissues were evaluated by hematoxy-lin-eosin(HE) staining. The protein expression of retinoic acid-inducible gene-Ⅰ(RIG-Ⅰ) and NOD-like receptor thermal protein domain associated protein 3(NLRP3) in lung tissues was detected by immunohistochemistry. The mRNA expression of RIG-Ⅰ, interferon regulatory factor 3(IRF3), interferon-beta(IFN-β), NLRP3, cysteinyl aspartate specific proteinase-1(caspase-1), gasdermin D(GSDMD), and interleukin-1 beta(IL-1β) in lung tissues was measured by quantitative real-time polymerase chain reaction(qRT-PCR). The protein expression of RIG-Ⅰ, IRF3, phosphorylated IRF3(p-IRF3), IFN-β, NLRP3, caspase-1, apoptosis-associated speck-like protein containing a CARD(ASC) and IL-1β in lung tissues was determined by Western blot. The results showed that, when compared with the virus-infected group, VTME intervention significantly alleviated pathological injury in the lung tissue. Immunohistochemical analysis revealed that VTME markedly reduced the protein expression of RIG-Ⅰ and NLRP3 in the lung tissue. Additionally, qRT-PCR and Western blot experiments indicated that VTME intervention significantly decreased the mRNA and protein expression of RIG-Ⅰ, IRF3, and IFN-β, decreased the mRNA expression of NLRP3, caspase-1, GSDMD, and IL-1β, and simultaneously reduced the protein expression of NLRP3, caspase-1, ASC, and IL-1β. Collectively, the mechanism of VTME on influenza virus-infected mice may be related to the regulation of innate immune response through inhibiting the expression of related genes and proteins in the RIG-Ⅰ signaling pathway and NLRP3 inflammasome signaling pathway.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 4","pages":"1110-1117"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147437748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Identification of \"pharmacokinetic effect markers\" of Buyang Huanwu Decoction against atherosclerosis based on network pharmacology, text mining, and molecular docking].","authors":"Li Jiang, Yu-Ting Zheng, Ya-Fen Ding, Wei-Wei Wang, Nian-di Zeng, Wei-Ting Yan, Kang Luo, Wang-Ping Xiong, Guo-Liang Xu","doi":"10.19540/j.cnki.cjcmm.20251119.201","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20251119.201","url":null,"abstract":"<p><p>This study integrated network pharmacology, text mining, and molecular docking to identify core bioactive components of Buyang Huanwu Decoction(BYHWD) against atherosclerosis(AS) and investigate their pharmacokinetic profiles in normal rats and the rat model of lipopolysaccharide(LPS)-induced chronic inflammation. Network pharmacology screening revealed 31 core components and 6 key targets(ESR1, PARP1, ALOX5, CNR2, NOS2, and PTGS2), enabling the construction of a herb-active component-target network for the anti-AS effects of BYHWD. Text mining supplemented high-frequency components reported in the literature, ultimately defining 8 critical pharmacokinetic effect markers: hydroxysafflor yellow A, amygdalin, albiflorin, paeoniflorin, ferulic acid, astragaloside Ⅳ, n-butylidenephthalide, and Z-ligustilide. Molecular docking demonstrated the binding energy below-5 kJ·mol~(-1) for all component-target pairs except hydroxysafflor yellow A-CNR2, indicating strong biological affinity. In the pharmacokinetic study, normal and model rats were administrated with BYHWD at a dose of 30 g·kg~(-1) by gavage. Then, plasma samples were collected at multiple time points and detected by the ultra performance liquid chromatography-triple quadrupole-mass spectrometry(UPLC-QQQ-MS) system. The results showed that compared with the normal group, the model group presented decreases in C_(max )and AUC_(0-t) and increases in t_(1/2), CL, and MRT_(0-t) of most components(P&lt;0.05). The t_(max) of 6 components showed a prolonged trend in the model group. Neither n-butylidenephthalide nor Z-ligustilide was detected in plasma samples due to concentrations below the lower limits of quantification(LLOQ). This multidimensional approach systematically elucidated the anti-AS pharmacodynamic basis and mechanism of BYHWD, establishing a novel methodology for investigating &quot;pharmacokinetic effect markers&quot; in TCM formulas.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 3","pages":"854-865"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147437357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Integrating network pharmacology and metabolomics to explore mechanism of Tricholoma matsutake extracts on alcoholic liver injury in mice].","authors":"Pei-Zhi Yuan, Cheng-Lun Tang, Qi Zhang, Tian-Yao Zhang, Jia-Xi Chen, Liu Yang, Zhi-Yan He, Xiao-Nian Cao, Yang Sun","doi":"10.19540/j.cnki.cjcmm.20251113.704","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20251113.704","url":null,"abstract":"<p><p>A metabolomics study was conducted on serum and liver samples from mice with alcoholic liver injury induced by the National Institute on Alcohol Abuse and Alcoholism(NIAAA). This was combined with network pharmacology to investigate the ameliorative effect of Tricholoma matsutake extracts on alcoholic liver injury in mice and its underlying mechanism. Serum levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), and triglyceride(TG) were measured, while liver pathological changes were assessed by hematoxylin-eosin(HE) staining. Moreover, differential metabolites in serum and liver were detected by gas chromatography-mass spectrometry(GC-MS), and potential targets of T. matsutake extracts were analyzed via network pharmacology. The predicted targets were compared with those associated with differential metabolites to explore key targets involved in the amelioration of alcoholic liver injury by the extract. The results showed that, compared with the pathological model group, the T. matsutake extracts treatment group showed significantly reduced serum ALT, AST, and TG levels, along with improved lipid vacuolation and hepatocyte morphology, indicating a significant ameliorative effect of the extract on alcoholic liver injury in mice. The metabolomics results revealed that the extract modulated 12 differential metabolites in liver tissues, including glucose, glutamate, leucine, and phenylalanine, and 31 differential metabolites in serum, including alanine, cholesterol, glucose, lactose, and methionine. Further network pharmacology analysis suggested that T. matsutake extracts may ameliorate alcoholic liver injury primarily by targeting pathways related to Myc and Cat, influencing genes such as Aldh9a1, Aldh3a2, Aldh2, Hsd17b4, Ehhadh, Acox1, Acaa1, Acot1, and Gls. These were mainly involved in five metabolic pathways: cysteine and methionine metabolism, arginine biosynthesis, and alanine, aspartate, and glutamate metabolism. In conclusion, T. matsutake extracts may ameliorate alcoholic liver injury in mice by regulating amino acid metabolism and glucose metabolism through pathways associated with Myc and Cat.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 3","pages":"782-790"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147437454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}