Zhongguo Zhongyao Zazhi最新文献

{"title":"[Investigating mechanism of salt-fried processed Plantaginis Semen active component calceolarioside B in intervening lipid peroxidation metabolic pathway to exert anti-renal fibrosis effects].","authors":"Jia-Hao Liu, Jia-Yi Xu, Qian-Xi Gu, Jia-Xuan Peng, Gang Cao, Lu Wang","doi":"10.19540/j.cnki.cjcmm.20260430.304","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260430.304","url":null,"abstract":"<p><p>This study employed an MCnebula-driven metabolomics approach to investigate the key biomarkers and metabolic pathways involved in the regulation of renal fibrosis by calceolarioside B. In a rat model of adenine-induced renal fibrosis, calceolarioside B was observed to significantly reduce serum creatinine and blood urea nitrogen levels. Western blot analysis showed that it markedly decreased the expression of fibrosis markers, including fibronectin(FN), hypoxia-inducible factor-1α(HIF-1α), and α-smooth muscle actin(α-SMA), in renal tissue. In vitro, calceolarioside B significantly inhibited the expression of FN, HIF-1α, vimentin, and α-SMA in transforming growth factor-β(TGF-β)-stimulated rat renal tubular epithelial cells(NRK-52E) and rat renal fibroblasts(NRK-49F). Metabolomics analysis showed that calceolarioside B significantly restored the levels of 47 serum metabolites in rats with renal fibrosis. Specifically, the content of fatty acids(e.g., N-acyl amides, and long-chain fatty acids) was increased, while that of lipids(lysophosphatidylcholine, phosphoethanolamine, and phosphatidylethanolamine) was decreased--a profile consistent with ACSL4-driven lipid peroxidation pathway. Based on these findings, key proteins and metabolites downstream of the lipid metabolism pathway were further validated. The results showed that, compared with the control group, rats with renal fibrosis exhibited significantly elevated serum levels of malondialdehyde(MDA), tumor necrosis factor-α(TNF-α), and interleukin-1β(IL-1β), while glutathione peroxidase 4(GPX4) and glutathione/oxidized glutathione(GSH/GSSG) were markedly reduced. calceolarioside B reversed these changes. In summary, these findings indicate that calceolarioside B can correct lipid metabolism abnormalities in the serum of rats with renal fibrosis by inhibiting the ACSL4-mediated lipid peroxidation pathway. Moreover, it can alleviate oxidative stress and inflammatory responses by activating the System Xc~--GSH-GPX4 antioxidant system, thereby ameliorating renal fibrosis.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 15","pages":"4331-4340"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Efficacy and safety of Salvianolic Acid for Injection treatment during recovery phase of ischemic cerebrovascular disease: clinical study based on single-arm objective performance criteria method].","authors":"Chen Zhang, Jing-Yang Wang, Min-Jing Luo, Mei-Juan Lu, Jian-Zhong Pang, Ou Li, Ye Li, Yu-Tong Fei, Qiang Xu","doi":"10.19540/j.cnki.cjcmm.20260514.501","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260514.501","url":null,"abstract":"&lt;p&gt;&lt;p&gt;The clinical treatment of ischemic stroke(IS) has long been plagued by the time window and complication risks of recanalization therapies, as well as the bleeding risk of antithrombotic therapy, making it urgent to explore safe and effective new regimens. Salvianolic Acid for Injection(SAFI), a TCM preparation with neuroprotective properties and the ability to improve cerebral blood flow, lacks large-sample clinical validation regarding its systematicness, efficacy, and safety of application in IS patients. This study relied on a clinical trial based on a multicenter, prospective, single-arm objective performance criteria method(registration No. ChiCTR1900026178), enrolling 2 203 IS patients with an onset time of 7-90 d and National Institutes of Health Stroke scale(NIHSS) scores of 4-20. All patients received intravenous infusion of SAFI at dose of 100 mg per day for 14 consecutive days. The primary efficacy indicator was the proportion of patients with a modified Rankin scale(mRS) scores of 0-1 at 90 d after treatment(preset target value of 35%). Secondary efficacy indicators include the proportion of patients with mRS scores recovers to 0-1 at 28 d after treatment, the comparison of NIHSS scores at 7, 14 d after treatment with the baseline, the proportion of patients with Barthel index(BI) scores≥75 at 7, 14, 28, and 90 d after treatment, and the comparison of MMSE scores with the baseline. The incidence, severity, and drug correlation of adverse events were evaluated via vital sign monitoring, laboratory examinations, etc. The binomial test(one-sided α=0.025) was used for primary endpoint analysis, and the 95%CI was calculated by the Clopper-Pearson. RESULTS:: show that among 2 127 patients who completed the trial, 47.91% of these patients(95%CI[45.77%, 50.06%]) achieve the primary endpoint, which is significantly higher than the preset objective value(P&lt;0.000 1). NIHSS scores of patients decrease significantly from baseline scores of(6.57±3.20) to(4.62±3.33) at 14 d after treatment(P&lt;0.000 1), with 23.79% of patients achieving scores improvement of ≥4 points or scores of 0-1. The proportion of patients with BI scores ≥75 increases from the baseline of 41.18% to 74.52% at 90 d after treatment(P&lt;0.000 1), and MMSE scores rises from baseline scores of(22.16±8.03) to(23.95±7.38) at 90 d after treatment(P&lt;0.000 1). In terms of safety, the incidence rates of adverse events and serious adverse reactions are 27.49% and 3.19%, respectively, with no fatal event reported. This study confirmed that SAFI can significantly improve neurological function, capabilities of daily living, and cognitive function in patients with mild to moderate IS at 7-90 d after onset, with a favorable safety profile. The trial design combining single-arm and objective performance criteria method employed in this study provides an innovative paradigm for the clinical evaluation of TCM injections and offers high-quality evidence for the clinical treatment of I","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 15","pages":"4502-4511"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Application of numerical simulation in modern manufacturing of traditional Chinese medicine preparations].","authors":"Ge Song, Yang Yu, Zheng Li","doi":"10.19540/j.cnki.cjcmm.20260430.301","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260430.301","url":null,"abstract":"<p><p>Since the implementation of the development strategy for traditional Chinese medicine(TCM) modernization, numerical simulation has become a vital technical tool for studying TCM manufacturing processes. Using computers to simulate and analyze complex physical phenomena and related equipment in pharmaceutical production contributes to optimizing process parameters, improving equipment performance, and enhancing product quality. This paper systematically reviews the application of numerical simulation in the manufacturing of pills, tablets, capsules, granules, and liquid formulations over the past decade. Furthermore, it discusses practical challenges in its application and offers corresponding development suggestions. The aim is to promote deeper integration of numerical simulation into the TCM manufacturing system and support high-quality development of the TCM industry.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 16","pages":"4537-4544"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Therapeutic mechanism of Astragalus polysaccharides in bladder dysfunction in DBA mice based on regulatory role of myosin Va in neurotransmission].","authors":"Hong-Liang Li, Na Zhou, Jia-Ye Liu, Sheng-Lian Ye, Wei-Ling Qu, Bo Tan, Hong-Ying Cao","doi":"10.19540/j.cnki.cjcmm.20260429.401","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260429.401","url":null,"abstract":"<p><p>This study aimed to investigate the therapeutic mechanism of Astragalus polysaccharides(APS) in improving bladder function in DBA mice by focusing on the repair of neurotransmission processes via the myosin Va pathway, specifically addressing its role in transport and docking of neurotransmitter vesicles. DBA mice were used to assess bladder dysfunction based on metabolic cage monitoring and urodynamics. The experiment included a control group, a DBA group, APS low-, medium-, and high-dose(0.256, 0.513, and 1.026 g·kg~(-1)) groups, and a positive control mecobalamin(0.195 g·kg~(-1)) group. After 3 weeks of administration, in vitro bladder detrusor muscle tension, voiding monitoring, and urodynamic assessment were performed. The expression of myosin Va and the synaptic vesicle marker protein synaptophysin was detected by RT-qPCR and immunofluorescence to further investigate the regulation of myosin Va and synaptophysin by APS. Bladder function was evaluated by in vitro detrusor tension experiments and urodynamics. The results showed that compared with the control group, the mRNA and protein expression levels of myosin Va and synaptophysin in the bladder of DBA mice were significantly decreased, suggesting that insufficient myosin Va expression led to impaired short-distance transport of vesicles. DBA mice displayed bladder contractile dysfunction, with significantly reduced contractile force upon electrical field stimulation; decreased voiding frequency and reduced voided volume; and urodynamic manifestations including prolonged voiding time, increased maximum bladder capacity, and markedly decreased maximum voiding pressure. APS treatment significantly improved bladder function in DBA mice by increasing voiding frequency, enhancing detrusor contractility, reducing residual urine volume, decreasing compliance, and significantly upregulating the mRNA and protein expression of myosin Va and synaptophysin. In conclusion, the expression of myosin Va and synaptic vesicle marker proteins in the bladder of DBA mice was significantly downregulated, which may lead to impaired neurotransmitter vesicle transport and docking, subsequently causing voiding dysfunction characterized by decreased maximum voiding pressure. APS can significantly upregulate the expression of myosin Va and synaptophysin, repair neurotransmission, thereby enhancing detrusor contractility and improving bladder function.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 15","pages":"4458-4465"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanism of total saponins from Allium macrostemon in reducing inflammatory infiltration of aortic macrophages and anti-atherosclerosis in ApoE~(-/-) mice via regulating NF-κB signaling pathway].","authors":"Ya-Nan Xu, Yi-Ting Lu, Sheng-Ping He, Xiang-Lian Chen, Hong-Fei Wu","doi":"10.19540/j.cnki.cjcmm.20260509.703","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260509.703","url":null,"abstract":"<p><p>This paper aims to investigate the effects of total saponins from Allium macrostemon Bunge(SAMB) on aortic plaques and inflammatory infiltration macrophages in ApoE~(-/-) mice with atherosclerosis(AS). SAMB components were analyzed by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS). AS animal models were established by 50 ApoE~(-/-) mice fed with a high-fat diet and randomized into a model group, low-, medium-, and high-dose SAMB groups, and a simvastatin group. Ten C57BL/6J mice were selected as the control group. The mice in the blank group and model group were given normal saline by intragastric administration, and the remaining groups were administered corresponding drugs via gavage daily. Aortic sinuses were stained with hematoxylin and eosin(HE) to measure the ratio of aortic plaque area to luminal area. Aortic inflammatory factors such as tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), monocyte chemoattractant protein-1(MCP-1), and interleukin-1β(IL-1β) were measured by enzyme-linked immunosorbent assay(ELISA). Macrophage infiltration areas were assessed by immunohistochemistry, and the protein expression levels of phosphorylated-nuclear factor-κB p65(p-NF-κB p65)/NF-κB p65 and inhibitor of nuclear factor α(IκBα) were assessed by Western blot. RAW264.7 macrophages were exposed to SAMB-containing serum with different concentrations. Proliferation ability was detected by EdU, and the migration ability of macrophages was detected by the wound healing assay and Transwell. The inflammatory factors, including TNF-α, IL-6, MCP-1, and IL-1β, were detected by ELISA; the levels of p-NF-κB p65/NF-κB p65 and IκBα were detected by Western blot; NF-κB p65 nuclear translocation was assessed by immunofluorescence. A total of thirty saponin components were identified from SAMB. Animal experiment results show that compared with the model group, low, medium, and high-dose SAMB groups significantly reduce aortic plaque area, aortic inflammatory factor levels, macrophage infiltration area, and protein expressions of p-NF-κB p65/NF-κB p65 in the vascular tissue of AS mice. Cell experimental results indicate that SAMB-containing serum can significantly inhibit LPS-induced abnormal proliferation and migration of RAW264.7 macrophages, reduce inflammatory factor release, decrease the levels of p-NF-κB p65/NF-κB p65 and IκBα, inhibit NF-κB p65 nuclear translocation, and block NF-κB signaling pathway activation. SAMB reduces inflammatory factors in vascular tissue of AS mice and inhibits macrophage infiltration in aortic plaques, exerting anti-atherosclerotic plaque formation effects. Its mechanism may be related to inhibiting the NF-κB-related pathway in macrophages.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 16","pages":"4744-4753"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[TCM digital human: an innovation engine driving TCM integrative pharmacology 2.0].","authors":"Hai-Yu Xu, Sen Li, Dan Teng, Ming-Bo Zhang, Yan-Hui Zhao, Ping Wang, Hong-Jun Yang","doi":"10.19540/j.cnki.cjcmm.20260522.401","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260522.401","url":null,"abstract":"<p><p>As the primary carrier of clinical treatment in traditional Chinese medicine(TCM), TCM formulae require elucidation of the interaction laws between their chemical substance basis and biological activities, which represents a key scientific issue in TCM research. The &quot;TCM integrative pharmacology&quot; theory previously proposed by our team has become an important paradigm in modern TCM research. However, current studies still face several limitations, including a static analytical perspective, insufficient spatiotemporal information, and unclear mechanisms of cross-organ coordinated regulation. Based on these challenges, this paper further proposes the innovative concept of a &quot;TCM digital human&quot;. Guided by the integrative pharmacology research paradigm, and drawing on advanced technologies such as virtual cells and virtual physiological human systems, this framework integrates artificial intelligence and multiscale modeling approaches to construct a computable virtual life system with TCM characteristics, ranging from virtual cells and virtual organs to a digital human. This system provides key support for the digital representation of TCM theory and for the scientific elucidation of the mechanisms underlying the therapeutic effects of TCM formulae.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 16","pages":"4517-4536"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Synthesis of derivatives of active compound from Ginkgo Semen and their anti-inflammatory activities].","authors":"Wei Li, Yu-Fei Miao, Qing-Xia Xu, Jun Zhang, Yan Liu, Chang Chen, An Liu, Cong Guo, Jin-Tang Cheng","doi":"10.19540/j.cnki.cjcmm.20260405.202","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260405.202","url":null,"abstract":"<p><p>GK-A is a new compound previously isolated by our research group from TCM Ginkgo Semen, which is traditionally used for relieving cough. Pharmacological results showed that it possessed good antitussive and anti-inflammatory activities, yet its low bioavailability is a significant problem. To search for candidate molecules with strong activity and high bioavailability, this study aimed to achieve this goal through structural modification. To evaluate the effects of different N-substituents in GK-A on the activity of active compounds, the study developed a concise synthetic route for these GK-A derivatives, synthesizing 15 derivatives(compounds 10-24) based on this route. Among these derivatives, compounds 10-21 were new compounds. An in vitro inflammatory model was established by inducing RAW264.7 cells with lipopolysaccharide(LPS). The cytotoxicity of different concentrations of the derivatives on mouse RAW264.7 macrophages was assessed using the CCK-8 assay to determine the appropriate dosing concentration. Nitric oxide(NO) levels in the cell supernatant were measured via the Griess assay, and the expression levels of inflammatory cytokines(interleukin-6(IL-6), tumor necrosis factor-α(TNF-α), and cyclooxygenase-2(COX-2) were detected by enzyme-linked immunosorbent assay(ELISA). The results showed that at a concentration of 100 μmol·L~(-1), all tested compounds significantly inhibited NO expression. Specifically, compounds 13 and 16 markedly reduced the expression levels of TNF-α, IL-6, and COX-2 in the cell supernatant. Compound 14 exhibited a significant inhibitory effect on TNF-α expression, while compound 17 significantly suppressed the expression of IL-6 and COX-2. These findings demonstrated that compounds 13, 14, 16, and 17 possessed promising anti-inflammatory activity in vitro. This discovery provides an experimental basis for screening antitussive drug candidates with high pharmacological activity, good bioavailability, and minimal side effects.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 15","pages":"4368-4376"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Two new indole alkaloids from Nelumbinis Folium and their hypolipidemic activities].","authors":"Xiao-Gen Ma, Xiao-Jing Wang, Hui-Yan Zhou, Jin-Jie Fan, Lin Zhang","doi":"10.19540/j.cnki.cjcmm.20260404.202","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260404.202","url":null,"abstract":"<p><p>Using modern chromatographic separation techniques such as silica gel, Sephadex LH-20, Toyopearl HW-40C column chromatography, and semi-preparative high-performance liquid chromatography, this study isolated 15 compounds from the 90% ethanol extract of Nelumbinis Folium. Their structures were identified by spectroscopic methods including ultraviolet(UV) spectroscopy, infrared(IR) spectroscopy, high-resolution electrospray ionization mass spectrometry(HR-ESI-MS), and nuclear magnetic resonance(NMR) spectroscopy. These compounds were identified as 1-(13,14-dimethoxybenzoyl)-3-[(8″E)-3″,4″,5″-trimethoxyphenylacetyl]-5-(1'-O-β-D-glucopyranosyl)-indole alkaloid(1), 2-(13-N-methyl-16,17-dimethoxyphenylacetyl)-3-(1'-O-β-D-glucopyranosyl)-7-methoxyindole alkaloid(2), isatindigoside A(3), isatindigoside B(4), wuzhuyuluckid A(5), wuzhuyuluckid B(6), wuzhuyuluckid C(7), talatensindoid B(8), talatensindoid C(9), isatisindigoticanine L(10), isatindigoside M(11), isatisindigoticanine M(12), isatisindigoticanine N(13), 1-methoxy-2-indoleacetonitrile(14), and 1-hydroxy-3-indoleacetonitrile(15). Among them, compounds 1-2 were determined as novel compounds, while compounds 3-15 were isolated from this plant for the first time. The novel compounds 1-2(50 μmol·L~(-1)) exhibited inhibitory effects on pancreatic lipase activity with inhibition rates of 68.38%±1.15% and 66.59%±1.13%, respectively. The novel compounds 1-2(50 μmol·L~(-1)) also suppressed the accumulation of triglycerides and neutral lipids in HepG2 cells with accumulation rates of 44.25%±0.95%, 52.81%±1.65% and 53.27%±0.97%, 67.08%±1.49%, respectively. Therefore, the novel compounds 1-2 demonstrated certain hypolipidemic activities.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 15","pages":"4360-4367"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanism of Shengqing Jiangzhuo Granules in ameliorating chronic functional constipation with dampness obstruction and Qi stagnation pattern and associated hyperuricemia based on \"gut-microbiota-barrier\" axis].","authors":"Yi-Xin Yang, Zi-Qing Xiao, Zhao-Chen Ma, Ming-Zhu Xu, Qian-Wen Lu, Ya Lin, Ping Wang, Hai-Yu Xu, Chang-Chuan Bai, Yan-Qiong Zhang","doi":"10.19540/j.cnki.cjcmm.20260206.707","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260206.707","url":null,"abstract":"<p><p>This study systematically investigated the efficacy and action mechanism of Shengqing Jiangzhuo Granules in ameliorating chronic functional constipation with dampness obstruction and Qi stagnation pattern and associated hyperuricemia by integrating pharmacodynamic evaluation for animals, multi-omics detection, and mechanistic validation. First, a rat model of chronic functional constipation with dampness obstruction and Qi stagnation pattern associated hyperuricemia was established by using a multi-factor modeling method. Animals were divided into a normal group, a model group, Shengqing Jiangzhuo Granules groups with low, medium, and high doses, linagliptin group, and prucalopride succinate group. Intestinal propulsion rate, histopathological changes, serum gastrointestinal hormones [substance P(SP), motilin(MTL), vasoactive intestinal peptide(VIP)], uric acid, and lipid metabolism indicators [total cholesterol(TC), triglycerides(TG)], were detected to comprehensively evaluate the efficacy. Subsequently, 16S rDNA sequencing and colonic content metabolomic analysis were performed to delineate changes in gut microbiota structure and metabolite profiles. Through multi-omics correlation analysis, the key regulatory pathways were identified. Finally, the hypoxia-inducible factor-1α(HIF-1α) protein and the distribution/expression of zonula occludens-1(ZO-1) and occludin in colon tissue were detected to verify the relevant mechanism. The results demonstrate that Shengqing Jiangzhuo Granules significantly improve intestinal propulsion of the model rat, repair tissue damage in the colon, spleen, and stomach, regulate gastrointestinal hormone disturbances, reduce uric acid in the serum, and correct lipid metabolism disorders. Moreover, the prescription can reverse gut microbiota dysbiosis, promote the proliferation of butyrate-producing microbiota(e.g., Faecalibacterium and Blautia), increase colonic butyrate content, activate the HIF-1α signaling pathway, and enhance the expression of tight junction proteins. In conclusion, this study gradually delves from holistic pharmacodynamics to microscopic mechanism, clarifying that the action mechanism of Shengqing Jiangzhuo Granules in exerting a multi-target and holistic improvement effect on chronic functional constipation with dampness obstruction and Qi stagnation pattern, as well as its associated hyperuricemia through regulating the &quot;gut microbiota-butyrate-HIF-1α-intestinal barrier&quot; axis, providing a scientific basis for its clinical popularization and application.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 15","pages":"4246-4256"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Exploring therapeutic mechanism of Compound Pien Tze Huang Tablets for adenoid hypertrophy in children based on network pharmacology and animal experiments].","authors":"Ying Zhang, Yi-Wei Sang, Qing-Qing Li, Feng-Rong Zhang, Zhen Zhen, Xian-Yu Li","doi":"10.19540/j.cnki.cjcmm.20260429.802","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260429.802","url":null,"abstract":"<p><p>This study aimed to systematically predict and elucidate the potential therapeutic targets and molecular mechanisms of Compound Pien Tze Huang Tablets(PZHT) in the treatment of adenoid hypertrophy(AH) in children by integrating network pharmacology with animal experiments. Chemical components of PZHT were retrieved from the TCMSP, HERB 2.0, and SwissADME databases, which identified 276 putative bioactive components with favorable pharmacokinetic properties. A total of 1 266 potential targets corresponding to these components were predicted. Meanwhile, 943 AH-related targets were retrieved from the GeneCards and OMIM databases. Intersection analysis yielded 316 common targets between PZHT and AH. The protein-protein interaction(PPI) network analysis further identified 10 core targets, including BCL2, ESR1, interleukin(IL)-6, GSK3B, EGFR, PARP1, KDR, MMP2, HSP90AA1, and SRC. GO functional annotation and KEGG pathway enrichment analyses suggested that PZHT might exert therapeutic effects mainly through inflammation-and immunity-related pathways, such as the tumor necrosis factor(TNF) and PI3K-Akt signaling pathways. On the basis of network pharmacology predictions, a rat model of AH was induced by ovalbumin(OVA) combined with lipopolysaccharide(LPS) for experimental validation. The results demonstrated that compared with the model group, PZHT groups at different doses showed significantly reduced frequencies of nasal rubbing and sneezing, with the middle-dose exhibiting the most pronounced improvement. In addition, the PZHT groups showed significant declines in the serum levels of inflammatory cytokines TNF-α and IL-6, as well as Th2-type immune response-related cytokines IL-4, IL-5, and IL-13, and the levels of allergy-related indicators, including OVA-specific immunoglobulin E(sIgE) and histamine. Histopathological examinations revealed that PZHT alleviated nasal mucosal injury, goblet cell hyperplasia, edema, and inflammatory infiltration, and suppressed abnormal lymphoid hyperplasia in the nasopharyngeal region. In conclusion, by combining network pharmacology analysis with animal experimental validation, this study demonstrates that PZHT exerts therapeutic effects on AH in children through multi-target and multi-pathway regulation. The mechanism may involve coordinated modulation of Th2-type immune responses and key inflammatory signaling pathways, which leads to attenuation of local inflammation and abnormal lymphoid tissue proliferation. These findings provide experimental evidence and a theoretical basis for the clinical application of PZHT in the treatment of AH in children.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 15","pages":"4410-4418"},"PeriodicalIF":0.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148889364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}