Zhongguo Zhongyao Zazhi最新文献

{"title":"[Exploring comorbidity mechanism of systemic lupus erythematosus with osteoporosis and progress in TCM intervention from perspective of kidney-bone-immune axis].","authors":"Yang Qin, Hua-Yan Tang","doi":"10.19540/j.cnki.cjcmm.20260518.501","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260518.501","url":null,"abstract":"<p><p>Patients with systemic lupus erythematosus( SLE) have a significantly elevated risk of developing osteoporosis, a condition driven by complex interactions among immune inflammation, hormonal imbalances, and medication-related factors. Current western therapies often struggle to concurrently address immune regulation and bone protection. Based on the theory that &quot; the kidney governs the bones and generates marrow&quot;, TCM characterizes the core pathogenesis as &quot; kidney deficiency and marrow emptiness as the root, with heat toxin and blood stasis obstruction as the manifestations &quot;. This concept aligns closely with the modern osteoimmunological concept of &quot; immune-skeletal system crosstalk&quot;, giving rise to the integrated Chinese-western medicine theoretical framework known as the kidney-bone-immune axis. This review systematically summarized contemporary pathological mechanisms and TCM understanding of the pathogenesis of osteoporosis in SLE patients. It elaborated on the scientific implications of the kidney-boneimmune axis and highlighted the application of herbal formulas within the TCM syndrome differentiation system, as well as the osteoimmune-regulatory mechanisms of single and compound TCM herbs. Currently, research in this field remains nascent, lacking high-quality evidence and systematic mechanistic exploration. Future efforts should deepen the disease-pattern combination research paradigm and establish a multi-level evidence system to promote the systematic integration and clinical translation of TCM in the prevention and management of osteoporosis in SLE patients.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"5103-5109"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[In vivo and in vitro component analysis of Panlongqi Tablets based on HPLC-Q-TOF-MS/MS].","authors":"Xiao-Xuan Li, Jia-Lin Wang, Qian-Qian Yan, Qing-Yu Zhao, Ying Liu, Na Lin, Chun-Fang Liu, Jing Zhang, Ya Lin","doi":"10.19540/j.cnki.cjcmm.20251205.201","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20251205.201","url":null,"abstract":"<p><p>In order to rapidly and accurately identify the in vivo and in vitro components of Panlongqi Tablets,this study employed HPLC-Q-TOF-MS/MS to conduct a qualitative analysis of the chemical components and the prototype compounds that enter the bloodstream. The chromatographic separation was carried out by gradient elution through an Agilent Zorbax SB C_(18) column(4. 6 mm × 250 mm,5 μm),with a mobile phase consisting of 0. 1% formic acid in water(A) and acetonitrile(B). The column temperature was maintained at 35 ℃,with a flow rate of 1. 0 m L·min~(-1). The mass spectrometry analysis was performed with an electrospray ionization(ESI) source,with both positive and negative ion scanning modes being employed to collect the mass spectral data. The study involved 12 male SD rats,which were randomly allocated into two groups: a blank group and a Panlongqi Tablets group. The rats in the Panlongqi Tablets group were administrated with a drug suspension at a concentration of 480 g·L~(-1) by gavage,while the rats in the blank group were administrated with an equal volume of distilled water. The drug was administered three times,and one hour after the third administration,blood plasma was collected from the rats. The plasma samples were processed and analyzed for their chemical components. By comparing the data with relevant information from the literature and reference standards,the in vitro and in vivo components were identified in both the positive and negative ion modes. The results showed that a total of 105 chemical components were identified in the extract of Panlongqi Tablets,including 28 terpenoids,19 organic acids,13 alkaloids,7 quinones,6 volatile oils,5 glycosides,8 phenylpropanoids,3 sugars,4 flavonoids,and 12 other compounds. In the rat plasma,30 prototype compounds and 27 metabolites were identified,with terpenoids and organic acids being the predominant components. This study successfully achieved the rapid characterization and identification of the chemical components and prototype compounds that enter the bloodstream from Panlongqi Tablets. The results provide valuable experimental evidence for studying the pharmacodynamic material basis and the mechanism of action of Panlongqi Tablets.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"4838-4847"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on mechanism of traditional Chinese medicine in treating acute lung injury based on endoplasmic reticulum stress].","authors":"Yi-Wei Chen, Yan-Wen Wang, Da-Cheng Tian, Yi-Xuan Bai, Jin-Hui Xu, Shuai-Chun Liu, Li-Xue Ma, Xing-Fang Li","doi":"10.19540/j.cnki.cjcmm.20260415.401","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260415.401","url":null,"abstract":"<p><p>Acute lung injury(ALI) is a severe pulmonary condition with a high mortality rate, and is also a leading cause of morbidity and mortality among intensive care unit patients. Endoplasmic reticulum stress(ERS) is a key central mechanism in ALI. It primarily regulates endoplasmic reticulum function by activating the unfolded protein response and modulating three core pathways-inositol-requiring enzyme 1(IRE1), activating transcription factor 6(ATF6), and protein kinase R-like endoplasmic reticulum kinase(PERK)-which in turn regulate processes such as oxidative stress, ferroptosis, and autophagy. It is involved in processes including apoptosis, inflammatory storm, and calcium homeostasis, thereby influencing the progression of ALI. TCM offers therapeutic advantages through its multi-targeted and multi-pathway approach, improving the course of ALI by modulating ERS. This paper reviewed the experimental evidence and mechanisms for single herbs and their chemical constituents or extracts(casticin, ursolic acid, toosendanin, resveratrol, ginsenosides, and total flavonoids from Patrinia villosa), TCM compound formulas(Zhenwu Decoction, Buzhong Yiqi Decoction, and Dushen Decoction), and Chinese patent medicines(Lianhua Qingwen Capsules and Jingfang Granules) to modulate ERS for the treatment of ALI. It elucidated the scientific rationale behind the use of TCM to modulate ERS for ALI treatment, providing a mechanistic basis and research directions for the prevention and treatment of ALI by integrated traditional Chinese and western medicine.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 18","pages":"5161-5169"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Supramolecular differences and pulmonary protective activity of Forsythiae Fructus-Lonicerae Japonicae Flos herb pair prepared with green Forsythiae Fructus and ripe Forsythiae Fructus].","authors":"Ji-Chang Wei, Gen Li, Yi-Hang Zhao, Yao Pu, Zhen-Song Wang, Cheng Huang, Yuan-Yuan Li, Xue-Mei Huang, Yan Ni, Peng-Long Wang","doi":"10.19540/j.cnki.cjcmm.20260714.301","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260714.301","url":null,"abstract":"<p><p>This study aimed to investigate the supramolecular differences arising from the herb pair Forsythiae Fructus-Lonicerae Japonicae Flos(LF) prepared with green Forsythiae Fructus( GF) versus ripe Forsythiae Fructus( RF), and to elucidate their respective pulmonary protective activities. The macroscopic morphology, chemical composition, and in vitro biological activities including antibacterial, antioxidant, and anti-fibrotic effects(specifically the inhibition of TGF-β1-induced fibroblast hyperproliferation and collagen deposition) were systematically compared between GF-LF and RF-LF co-decoctions. The results revealed significant morphological distinctions: the GF-LF co-decoction exhibited higher turbidity and self-assembled spherical supramolecular particles with a diameter of approximately 1 μm, whereas the RF-LF co-decoction formed smaller particles with a diameter ranging from 400 to 600 nm. HPLC and UPLC-MS/MS analyses indicated that both co-decoctions exhibited qualitatively similar phytochemical profiles. However, quantitative differences were evident: GF-LF showed higher content of forsythoside A, phillyrin, and rutin, while RF-LF exhibited higher levels of chlorogenic acid and phillygenin. Biological activity assays demonstrated that GF-LF possessed significantly stronger antibacterial, antioxidant, and anti-fibrotic activities than RF-LF. This study reveals, at the supramolecular level, that the differences in component content between GF and RF drive distinct self-assembly behaviors in the decocting with LF, consequently influencing their pulmonary protective efficacy. These findings provide an experimental basis for the rational clinical selection of Forsythiae Fructus specifications.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"4895-4905"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanistic study on Kaixin Powder ameliorating chronic stress-induced CD8~+T cell exhaustion and delaying lung adenocarcinoma progression by modulating IDO1/Kyn/Ahr axis].","authors":"Xi Fu, Yu-Ting Bai, Jia-Wei He, Yu-Xuan Xiong, Xing-Yue Liu, Chun-Xia Huang, Tian-Yao Gong, Heng-Zhou Lai, Xue-Ke Li, Chuan Zheng, Feng-Ming You, Qiong Ma","doi":"10.19540/j.cnki.cjcmm.20260610.701","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260610.701","url":null,"abstract":"&lt;p&gt;&lt;p&gt;This study aimed to investigate the interventional effects of Kaixin Powder(KXP) on the progression of lung adenocarcinoma(LUAD) under chronic stress and its underlying mechanism. Ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was employed to characterize the chemical constituents of KXP, followed by network pharmacology and molecular docking analyses to screen for its potential targets. A chronic stress-associated lung cancer mouse model was established by axillary inoculation of Lewis lung carcinoma(LLC) cells combined with chronic unpredictable mild stress(CUMS). Sixty SPF-grade C57BL/6 mice were randomly divided into the following groups: normal group, lung cancer group, model group, low-and high-dose KXP(13.65 and 27.3 g·kg~(-1)) groups, and fluoxetine(2 mg·kg~(-1)) group. Tumor growth indicators, including body weight, tumor mass, and tumor inhibition rate of mice were measured. Depression-like behaviors were observed by open field test(OFT) and sucrose preference test(SPT). Serum levels of stress factors such as corticosterone(Cort) and kynurenine(Kyn) were measured by ELISA. Histopathological changes in tumor tissue were assessed by hematoxylin-eosin(HE) staining. Peripheral blood T lymphocyte subsets were analyzed by flow cytometry. Intratumoral CD8~+T cells infiltration was detected by immunofluorescence, and the protein expression levels of indoleamine 2,3-dioxygenase 1(IDO1) and aryl hydrocarbon receptor(Ahr) were determined by Western blot. Serum ALT, AST, BUN, and Scr levels were measured to evaluate hepatic and renal safety. UPLC-MS/MS analysis characterized 157 chemical constituents of KXP. The protein expression levels of indoleamine 2,3-dioxygenase 1(IDO1) and aryl hydrocarbon receptor(Ahr) were detected by Western blot. In addition, serum ALT, AST, BUN, and Scr levels were measured to evaluate hepatic and renal safety. UPLC-MS identified 157 chemical constituents of KXP. Network pharmacology analysis obtained 367 potential targets, among which epidermal growth factor receptor(EGFR), protein kinase B alpha(AKT1), signal transducer and activator of transcription 3(STAT3), heat shock protein 90 alpha family class A member 1(HSP90AA1), and IDO1 were screened as core targets. Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis and molecular docking indicated that abnormal tryptophan metabolism mediated by IDO1 may be a potential mechanism underlying KXP intervention in LUAD complicated with depression. Animal experiments showed that, compared with the lung cancer group, the model group exhibited significantly accelerated tumor growth, increased structural disarray and atypia in HE-stained tumor tissue, obvious depression-like behaviors, and significantly elevated serum Cort and Kyn levels. In addition, the proportions of peripheral blood CD3~+ and CD3~+CD4~+T cells and the CD3~+CD4~+/CD3~+CD8~+ ratio significantly decreased, whereas the proportion of CD3~+CD8~+T cells significantly incr","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 18","pages":"5373-5384"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on clinical application and action mechanism of TCM in prevention and treatment of chronic obstructive pulmonary disease].","authors":"Qing-Ting Meng, Hong-Yu Zhu, Jia-Yi Hu, Cheng Yang, Jia-Bing Tong, Ze-Geng Li, Qin-Jun Yang","doi":"10.19540/j.cnki.cjcmm.20260520.501","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260520.501","url":null,"abstract":"<p><p>The pathological mechanisms of chronic obstructive pulmonary disease(COPD) are complex, and current intervention strategies are difficult to control the overall progression of the disease. Guided by the treatment principle of syndrome differentiation and treatment, TCM treats COPD based on the core pathogenesis of deficiency in origin and excess in superficiality, showing the unique advantages of multiple targets and holistic regulation. This paper systematically summarized the pathological mechanisms of COPD and the clinical and basic research progress of TCM treatment. Based on the comprehensive analysis of the etiology, pathogenesis, and modern pathological changes of COPD, this paper also summarized the clinical efficacy of TCM in the prevention and treatment of COPD and elaborated its action mechanism in depth. Clinical studies have shown that TCM compound formulas with the effects of tonifying the lungs and strengthening the spleen, nourishing the lungs and kidneys, clearing heat and dissolving phlegm, as well as external treatment methods such as acupuncture, acupoint application, and acupoint injection, can effectively improve the symptoms of cough, phlegm, shortness of breath, reduce the number of acute exacerbations, improve lung function and exercise tolerance, and significantly improve the quality of life of patients. At the level of basic research, a variety of active components such as flavonoids, saponins, and polysaccharides contained in TCM, as well as compound formulas play a protective role by intervening with multiple signaling pathways and targets, including nuclear factor-κB(NF-κB)/NOD-like receptor family pyrin domain-containing 3(NLRP3), transforming growth factor-β(TGF-β)/Smad, nuclear factor erythroid 2-related factor 2(Nrf2)/heme oxygenase-1(HO-1), and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt), thereby exerting multi-pathway protective effects in inhibiting inflammatory responses, alleviating oxidative stress, improving airway remodeling, regulating protease-antiprotease imbalance, and modulating programmed cell death. In addition, this paper pointed out the shortcomings in the current research field, aiming to provide new theoretical bases and research ideas for the comprehensive prevention and treatment of COPD and the modernized research of TCM.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 18","pages":"5170-5184"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanism of total iridoids from Gardeniae Fructus in treating ulcerative colitis based on \"bitterness drying dampness\" theory].","authors":"Xiao-Mei Fu, Rui-Xiang Xu, Yu-Ying Fan, Zhou Zhang, Shu-Yao Wu, Jing Tang, Han-Yue Huang, Yi-Qing Hu, Chao Yang, Zhi-Gui Wu, Jing Liu","doi":"10.19540/j.cnki.cjcmm.20260203.402","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260203.402","url":null,"abstract":"<p><p>This study aimed to explore the mechanism of total iridoids from Gardeniae Fructus in treating ulcerative colitis(UC) in rats from the perspective of the &quot; bitterness drying dampness&quot; theory in TCM. SD rats were randomly divided into a blank group, a model group, groups of high-and low-dose total iridoids from Gardeniae Fructus(200 and 100 mg·kg~(-1), respectively), and a positive drug group, with 10 rats per group. Except for the blank group, UC model was induced in all other groups via enema administration of 2,4, 6-trinitrobenzene sulfonic acid(TNBS) solution(100 mg·kg~(-1)). During the administration period, the appearance of the rats was observed and recorded daily, and the disease activity index(DAI) was scored. Colon length and spleen weight were measured, and the colon mucosal damage index(CMDI) was scored. Microscopic pathological damage in the colon was observed via HE staining and AB staining, and the histopathological index(HI) was scored. The levels of inflammatory cytokines tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), interleukin-10(IL-10), and 3',5'-cyclicade-nosine monophosphate(cAMP) in colon tissue were detected by ELISA. The expression levels and distribution/damage of intercellular tight junction proteins E-cadherin, claudin-1, and occludin in the colon mucosal layer were detected using Western blot and immunofluorescence. The expression levels of bitter taste receptor 38(TAS2R38), phospholipase C β2( PLCβ2), inositol 1,4,5-trisphos-phate( IP3), and α-gustducin(GNAT3) were detected by Western blot and RT-qPCR. The results showed that compared with the blank group, rats in the model group exhibited a significant decrease in body weight and a significant increase in DAI score. Furthermore, colon length was significantly shortened, while the spleen index, CMDI, and HI scores were all significantly increased. Concomitantly, the levels of TNF-α and IL-6 in colon tissue were significantly elevated, while IL-10 levels were significantly decreased. Additionally, the protein expression levels of E-cadherin, claudin-1, and occludin were significantly reduced. The protein expression level of TAS2R38 was significantly downregulated, and the protein and mRNA expression levels of PLCβ2, IP3, GNAT3 in colon tissue were significantly downregulated, and the content of cAMP was significant reduced. Administration of total iridoids from Gardeniae Fructus significantly improved all the above indicators in UC rats. These treatments significantly enhanced TAS2R38 protein expression in colonic tissues, restored the protein and mRNA expression of PLCβ2, IP3 and GNAT3, and raised cAMP content. In conclusion, total iridoids from Gardeniae Fructus may alleviate colonic epithelial barrier damage and inflammatory responses by activating TAS2R38 and regulating the GNAT3/cAMP and PLCβ2/IP3 signaling pathways, thereby improving symptoms in UC rats.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"5018-5026"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress in modern scientific connotation of compatibility of TCM compound prescriptions based on supramolecular assembly].","authors":"Shuang-Chen Zhang, Nian-Zhan Zhang, Xiao-Yu Huang, Chao-Ying DU, Cheng-Ying Shen, Bao-de Shen","doi":"10.19540/j.cnki.cjcmm.20260714.306","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260714.306","url":null,"abstract":"<p><p>The compatibility of TCM compound prescriptions is a core component of the TCM theory. Clarifying the modern scientific connotation of the compatibility theory is of great significance for promoting the modern development and clinical application of TCM compound prescriptions. In recent years, interdisciplinary research between supramolecular chemistry and TCM has gradually become a research hotspot. Studies have confirmed that the chemical components in TCM compound prescriptions can form supramolecular aggregates with specific structures and functions through molecular self-assembly, which serve as an important physical structural basis for the efficacy of TCM compound prescriptions. This can partially unravel the intrinsic mechanisms underlying the holistic synergy, toxicity attenuation, and efficacy enhancement of TCM compatibility. By reviewing the research progress in TCM supramolecular assembly, this paper elaborates on the basic characteristics of TCM supramolecular assembly. Furthermore, this paper systematically reviews the research advances in interpreting the connotations of efficacy enhancement and toxicity attenuation as well as the efficacy attenuation and toxicity enhancement(incompatibility) of the compatibility of TCM compound prescriptions via supramolecular assembly. This review is expected to provide a reference for further clarifying and enriching the modern scientific connotation of the compatibility theory and to promote the modern development and clinical application of TCM compound prescriptions.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"4878-4885"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research on mechanism of Panlongqi Tablets regulating β2-adrenergic receptors to improve early fracture injury].","authors":"Jia-Lin Wang, Xiao-Xuan Li, Chu-Pei Zheng, Shuang-Rong Gao, Qian-Qian Yan, Qing-Yu Zhao, Jia-Yun Shen, Yan-Qiong Zhang, Teng-Fei Han, Cong-Cong Sun, Wen-Jie Li, Chun-Fang Liu, Na Lin","doi":"10.19540/j.cnki.cjcmm.20260611.901","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260611.901","url":null,"abstract":"&lt;p&gt;&lt;p&gt;This study aims to clarify the alleviating effects of Panlongqi Tablets(PLQP) on early fracture injuries and to reveal the mechanism of action through a multi-dimensional research method integrating network pharmacology,molecular docking,surface plasmon resonance(SPR),and animal experiments. Firstly,SD rats were randomized into sham operation,model,low-,medium-,and high-dose(0. 16,0. 32,and 0. 64 g·kg~(-1),respectively) PLQP,and positive control(Jiegu Qili Capsules,0. 10 g·kg~(-1)) groups,with 12 rats in each group. Except the sham operation group,other groups were modeled for open transverse fracture of the tibia. After modeling,administration groups received corresponding drugs by gavage for 7 days. The efficacy was comprehensively evaluated in terms of local tenderness,mechanical pain,swelling degree,skin temperature,coagulation function,gait,and histopathological changes of fractures. Then,the fracture transcriptome dataset(GSE262132) from the GEO database and the corresponding targets of the blood-absorbed components of PLQP were collected. By comparison among different time points of fractures,the strongest timeeffect intervention targets of PLQP were predicted,and they were then verified by molecular docking,SPR,and animal experiments. The results showed that PLQP significantly reduced the local swelling and skin temperature in the early stage of fractures,increased the mechanical pain threshold and local tenderness threshold of the affected limb,improved the gait and coagulation function of the affected limb,reduced inflammatory cell infiltration in the callus,lowered the content of fibrous connective tissue,increased the deposition of cartilage and collagen fibers,and promoted angiogenesis in model rats. Network pharmacological analysis indicated that β2-adrenergic receptor(ADRB2),interleukin(IL)-1β,endothelial nitric oxide synthase(NOS3),tumor necrosis factor(TNF),and endothelin-1(EDN1) were candidate strong targets for PLQP intervention in the early stage(7 days) of fractures. Among them,ADRB2 and EDN1 were specific. The results of molecular docking and SPR showed that the blood-absorbed components of PLQP,such as cryptotanshinone,11-keto-β-boswellic acid,tetrahydroberberine,β-ecdysterone,and tectorigenin,all had good binding activities with ADRB2. The results of immunohistochemistry,immunofluorescence,Western blot,and ELISA showed that PLQP upregulated the coexpression of macrophage CD68~+ADRB2~+,osteoblast Osterix~+ADRB2~+,and vascular endothelial cell CD31~+ADRB2~+ in fracture callus,down-regulated the protein level of the M1 macrophage marker i NOS and inhibited the secretion of IL-1β,IL-6,and TNF-α,increased the level of the M2 macrophage marker CD206,and up-regulated the secretion of IL-10. In addition,PLQP increased the expression levels of VEGF and e NOS and the ratio of encoding gene EDN1(ET-1) to nitric oxide(NO). In conclusion,PLQP can significantly alleviate the redness,swelling,heat,and pain symptoms and functional d","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 17","pages":"4848-4859"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparative analysis of chemical constituents between wild and cultivated Paeoniae Radix Rubra from authentic producing areas based on UHPLC-Q-TOF-MS combined with multivariate statistical analysis].","authors":"Xi-Jie Tian, Yan Fang, Lan Yao, De-Xin Yu, Yi Nan, Xiao-Juan Chen, Juan Song, Bao-Lin Guo, Hai-Zhen Liang, Bai-Ping Ma","doi":"10.19540/j.cnki.cjcmm.20260512.201","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20260512.201","url":null,"abstract":"<p><p>To clarify the similarities and differences in chemical constituents between wild and cultivated Paeoniae Radix Rubra from traditional authentic producing areas, and to investigate the quality status of cultivated Paeoniae Radix Rubra as a new production model of medicinal materials, this study employed ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF-MS) to characterize and identify the chemical constituents in wild and cultivated medicinal materials derived from two original species of Paeoniae Radix Rubra(Paeonia lactiflora and P. veitchii) collected from authentic producing areas in Inner Mongolia and Sichuan. Multivariate statistical analysis was further applied to screen differential components between wild and cultivated samples. A total of 60 chemical compounds were identified from Paeoniae Radix Rubra, including monoterpene glycosides, flavonoids, tannins, phenolic acids, and other structural types. The multivariate statistical analysis showed that there were no obvious differences in the composition profile and overall content between wild and cultivated Paeoniae Radix Rubra, with differences mainly observed in the content of certain components. From a chemical perspective, this study demonstrates the similarity between cultivated and wild Paeoniae Radix Rubra from authentic producing areas and provides a reference for the quality control of cultivated Paeoniae Radix Rubra.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 18","pages":"5289-5303"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149185994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}