Zhongguo Zhongyao Zazhi最新文献

{"title":"[A new sesquiterpene alkaloid and a new caffeoylquinic acid derivative from roots of Saussurea lappa].","authors":"Mao-Xin Wang, Juan Huang, Li-Fen Cao, Jin Ouyang, Qi-Liang Ouyang, Jing Guo, Robertson Luke, Wen Xu","doi":"10.19540/j.cnki.cjcmm.20251225.201","DOIUrl":"10.19540/j.cnki.cjcmm.20251225.201","url":null,"abstract":"<p><p>The chemical components in the roots of Saussurea lappa were investigated via rapid separation technologies such as FLASH diol gel and semi-preparative liquid chromatography. The planar structures and absolute configurations of the compounds were determined by analysis of their physicochemical properties, HR-ESI-MS, UV-Vis, ECD and NMR spectroscopic data. Nine compounds were isolated from the root extract of S. lappa, and they were identified as saussureamine I(1), saussurenin A(2), dehydrocostus lactone(3), saussureamine B(4), saussureamine C(5), saussureamine F(6), picriside B(7), 3,4-dicaffeoylquinic acid(8), and arbusculin A(9). Among them, compounds 1 and 2 were novel compounds.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 5","pages":"1373-1379"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Construction and transdermal delivery characteristics evaluation of self-assembled gentiopicroside-glycyrrhizic acid micelles].","authors":"Wen-Ting Zha, Li Chen, Wen-Jie Ning, Yu-Ye Xue, Ling-Yu Hang, Hai-Long Yuan","doi":"10.19540/j.cnki.cjcmm.20251215.301","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20251215.301","url":null,"abstract":"<p><p>To address the critical challenge of poor skin permeability in the transdermal delivery of gentiopicroside(GPS), this study utilized the active herbal component glycyrrhizic acid(GA) as a natural carrier to construct self-assembled gentiopicroside-glycyrrhizic acid micelles(GPS-GA micelles), aiming to enhance the transdermal delivery efficiency of GPS and investigate its transdermal behavior. The micelles were prepared via the medium milling method. Techniques including UV spectroscopy, FT-IR, DSC, and XRD were employed to characterize micelle formation and the interactions between GPS and GA. In vitro and in vivo transdermal experiments were conducted to evaluate drug permeation and skin distribution behavior, which was combined with histological analysis to explore the permeation enhancement mechanism. The prepared micelles exhibited uniform spherical morphology with a particle size of(58.13±8.81) nm, a polydispersity index(PDI) of 0.249 7±0.077 6, and the zeta potential of(-30.01±0.85) mV. In vitro permeation studies demonstrated that GPS-GA micelles significantly enhanced both the cumulative permeation amount and permeation flux of GPS compared to a GPS solution. In vivo transdermal studies utilizing P4 and C6 fluorescently labeled hybrid micelles demonstrated distinct follicular targeting and accumulation characteristics of micelles via fluorescence tracking. The micelles were detectable, forming a drug reservoir that facilitated sustained release and deep permeation. This study provides a novel strategy for improving the transdermal delivery of soluble active components from TCM.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 5","pages":"1334-1343"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanism study on treating psoriasis of blood-heat type with Tuhuaidan Siwu Decoction based on metabolomics and p38 MAPK/NF-κB signaling pathway].","authors":"Si-Yuan Li, Xiao-Jie Fan, Teng-Zhen Jiao, Hai-Yan He, Yu-Bing Zhang, Ying Jia","doi":"10.19540/j.cnki.cjcmm.20251121.901","DOIUrl":"https://doi.org/10.19540/j.cnki.cjcmm.20251121.901","url":null,"abstract":"&lt;p&gt;&lt;p&gt;This study aims to investigate the effects of Tuhuaidan Siwu Decoction on serum metabolomics and p38 mitogen-activated protein kinase(p38 MAPK)/nuclear factor kappa B(NF-κB) signaling pathway in propranolol-induced mouse model of psoriasis with syndrome, and to investigate its mechanism of action. Forty KM mice were randomly divided into a blank group, a model group, a positive group, and a treatment group, with 10 mice in each group. The psoriasis model was induced by 5% propranolol hydrochloride, and the blood heat syndrome model was established by intragastric administration of Zingiberis Rhizoma and Glycyrrhizae Radix et Rhizoma decoction combined with ultraviolet lamp irradiation. After 7 days of modeling, the treatment group and the positive group were given corresponding drug interventions, while the blank group and the model group were given normal saline, for 14 consecutive days. The skin lesions of mice were recorded and scored using the psoriasis area and severity index(PASI). Spleen index was detected. Pathological changes of skin lesion tissues were observed by hematoxylin-eosin(HE) staining and scored by Baker scores. The expression of tumor necrosis factor-α(TNF-α), interleukin(IL)-1β, IL-6, and IL-17 in mouse serum was detected by enzyme-linked immunosorbent assay(ELISA). The expression of p38 MAPK, p-p38 MAPK, inhibitor of nuclear factor kappa B alpha(IκBα), NF-κB p65, and p-NF-κB p65 was detected by Western blot. Serum metabolomics analysis of mice was performed to screen differential metabolites and psoriasis-related metabolic pathways. The results showed that compared with the blank group, the model group showed significantly increased PASI score and spleen index, manifestations of parakeratosis or hyperkeratosis, acanthosis, inflammatory cell infiltration in skin histopathology, a significantly increased Baker score, significantly increased expression levels of TNF-α, IL-1β, IL-6, and IL-17, significantly up-regulated phosphorylation levels of p38 MAPK and NF-κB p65, and a significantly down-regulated IκBα level. Compared with the model group, the positive group and the treatment group exhibited significantly decreased PASI scores and spleen indices, improved skin pathological changes, significantly decreased Baker scores, significantly decreased expression levels of TNF-α, IL-1β, IL-6, and IL-17, significantly down-regulated phosphorylation levels of p38 MAPK and NF-κB p65, and significantly up-regulated IκBα levels. Serum metabolomics showed that there were 42 differential metabolites between the blank group and the model group, with 21 of them up-regulated and 21 down-regulated, which were related to metabolic pathways such as glycerophospholipid metabolism, arachidonic acid metabolism, citrate cycle(TCA cycle), and valine, leucine, and isoleucine degradation. There were 38 differential metabolites between the model group and the treatment group, with 15 of them up-regulated and 23 down-regulated, which were mainly r","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 6","pages":"1757-1765"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Shared mechanisms of obesity and male infertility and prediction of natural therapeutics based on machine learning and single-cell sequencing].","authors":"Zhuo-Zhi Gong, Qiu-Jian Feng, Qing-He Gao, Fu Wang, Jun Guo, Sheng-Jing Liu","doi":"10.19540/j.cnki.cjcmm.20251114.901","DOIUrl":"10.19540/j.cnki.cjcmm.20251114.901","url":null,"abstract":"<p><p>The incidence of obesity and male infertility continues to rise, and a complex pathophysiological association exists between the two. However, the comorbid molecular mechanisms remain incompletely elucidated. In this study, bioinformatics and machine learning methods were integrated to systematically explore the shared mechanisms of obesity and male infertility, and to predict natural compounds and TCMs with therapeutic potential based on multi-omics data. Datasets of male infertility and obesity were obtained from the GEO database, and 43 intersecting genes were identified through differential expression analysis. Using three machine learning algorithms, including random forest, least absolute shrinkage and selection operator(LASSO) regression, and support vector machine(SVM), five Hub genes(MAP4K4, GPT2, ADSSL1, PAIP2, and GINS3) were screened. The combined diagnostic model achieved an area under the curve(AUC) greater than 0.8, indicating good diagnostic performance. Functional enrichment analysis revealed that these genes are mainly involved in key biological processes such as amino acid metabolism, cell migration, and DNA replication. Immune infiltration analysis showed a significant upregulation of central memory CD4~+ T cells in both diseases, suggesting that chronic immune activation is an important basis for their comorbidity. Single-cell sequencing and pseudotime analyses demonstrated disordered cell differentiation trajectories in the adipose tissue of obese patients and the testicular tissue of infertile patients. Based on the connectivity map(CMap) database, ten natural compounds, including berberine, curcumin, and ginsenosides, were predicted and further validated by molecular docking to have strong binding affinities with the Hub genes. Integration with the traditional Chinese medicine systems pharmacology(TCMSP) platform enabled the construction of a &quot;target-natural compound-herbal medicine&quot; interaction network, identifying 38 corresponding herbal medicines. Property and meridian analysis indicated a predominance of warm nature, sweet flavor, and liver meridian tropism, consistent with the TCM therapeutic principles of &quot;resolving phlegm and dampness, strengthening the spleen, and tonifying the kidney&quot;. This study reveals, at the molecular level, the comorbid mechanisms of obesity and male infertility in immune-inflammatory regulation and cell differentiation dysfunction, providing a theoretical basis and potential drug targets for precise TCM intervention in obesity-related male infertility.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 6","pages":"1766-1774"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Protective effects and mechanisms of Sanguisorbae Radix Carbonisata nano-components against ulcerative colitis].","authors":"Min-Long Xia, Yan Huang, Xiao-Peng Li, Ming-Min Jiang, Hong-Hua Li, Yun Zhao, En-Li Wang, Jing-Chun Yao, Guo-Liang Cheng, Hui-Hua Qu, Yan Zhao, Hui Kong","doi":"10.19540/j.cnki.cjcmm.20251110.902","DOIUrl":"10.19540/j.cnki.cjcmm.20251110.902","url":null,"abstract":"<p><p>This study aims to isolate and extract Sanguisorbae Radix Carbonisata nano-components(SRC-NCs) from Sanguisorbae Radix Carbonisata(SRC) and investigate their protective effects against ulcerative colitis(UC) and the underlying mechanisms. The SRC-NCs were systematically characterized by nanomaterial characterization techniques to analyze their morphological structure, optical properties, and characteristics of functional groups on the surface. Meanwhile, a dextran sulfate sodium(DSS)-induced UC mouse model was established to evaluate the general condition, disease activity index(DAI) score, and pathological damage degree of the colon tissue of mice after SRC-NCs intervention. The level or activity of inflammatory factors(interleukin(IL)-17A, IL-6, IL-10, IL-2, tumor necrosis factor alpha(TNF-α), and IL-1β) and oxidative stress markers(myeloperoxidase(MPO), malondialdehyde(MDA), superoxide dismutase(SOD), glutathione(GSH), and nitric oxide(NO)) in the colon tissue was detected. The expression level of key proteins, such as Toll-like receptor 4(TLR4), myeloid differentiation primary response protein 88(MYD88), and nuclear factor-kappa B p65(NF-κB p65), in the colon tissue was detected by Western blot to provide key molecular evidence for revealing the intervention mechanism of SRC-NCs. The results showed that SRC-NCs were nearly spherical under transmission electron microscope(TEM), with a uniform particle size distribution(0.8-2.6 nm), a lattice spacing of 0.17 nm, and multiple active genes such as hydroxyl, amino, and carboxyl groups on the surface. In the UC model, SRC-NCs could slow down the weight loss and increase in DAI score of mice, and alleviate the shortening of the colon and the degree of tissue damage. SRC-NCs could down-regulate the levels of pro-inflammatory factors such as IL-17A, IL-6, IL-2, TNF-α, and IL-1β in the colon tissue, up-regulate the level of anti-inflammatory factor IL-10, reduce the activity of MPO and the content of MDA and NO, increase the activity of SOD and the level of GSH, and inhibit the expression of TLR4/MYD88/NF-κB p65 proteins. In conclusion, the study for the first time demonstrates that SRC-NCs are the key active components of SRC in exerting protective effects against UC, and the mechanism may be related to the inhibition of inflammatory responses of the TLR4/MYD88/NF-κB pathway and the reduction of oxidative stress.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 5","pages":"1454-1463"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Variation of amino acid components and taste correlation analysis during stir-frying process of Gardeniae Fructus].","authors":"Lin Yan, Meng-Ru Qiao, Ya-Nan Song, Zhe Jia, Yun Wang, Cun Zhang","doi":"10.19540/j.cnki.cjcmm.20251103.302","DOIUrl":"10.19540/j.cnki.cjcmm.20251103.302","url":null,"abstract":"<p><p>This study investigated the dynamic changes of twelve amino acids during the processing of Gardeniae Fructus Praeparatus(Jiao Zhizi) and their association with taste properties, so as to provide a scientific basis for the underlying processing mechanism and quality assessment. Samples obtained during the stir-frying process were analyzed using an electronic tongue to quantify taste characteristics, and the contents of twelve amino acids were determined by pre-column derivatization with phenyl isothiocyanate(PITC) followed by high performance liquid chromatography(HPLC) via chemometric analysis. Correlation heatmaps were generated to explore the relationship between amino acid profiles and taste attributes. The electronic tongue results revealed an increase in bitterness(including bitter aftertaste and acidic bitterness) and astringency during the later stages of processing, while umami and richness decreased. Total amino acid content initially increased and subsequently decreased, with arginine and lysine undergoing the most substantial degradation. Chemometric analysis classified the processing into three distinct stages, identifying phenylalanine, threonine, glycine, lysine, arginine, isoleucine, and tyrosine as key amino acids indicative of the processing degree. Amino acids were significantly positively correlated with umami and richness and negatively correlated with bitterness. Arginine showed the strongest positive contribution to umami and the most significant suppressive effect on bitterness. During processing, amino acids were extensively consumed and degraded via Maillard and Strecker reaction, serving as critical precursors responsible for the enhancement of bitterness and the reduction of umami.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 6","pages":"1604-1614"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Two new compounds from Cyclocarya paliurus fruits and their α-glucosidase inhibitory activity].","authors":"Qi-Qi Hu, Ling-Yan Dai, Qin Huang, Yue-Qing Zhu, Jin-Bo Xiao, Li-Hong Tan, Han-Wen Yuan, Wei Wang, Yu-Qing Jian","doi":"10.19540/j.cnki.cjcmm.20251203.201","DOIUrl":"10.19540/j.cnki.cjcmm.20251203.201","url":null,"abstract":"<p><p>This study aims to isolate and identify the chemical constituents of Cyclocarya paliurus fruits. The constituents were isolated and purified by silica gel, Sephadex LH-20, and semi-preparative high performance liquid chromatography. Their structures were elucidated by a combination of IR, UV, HR-ESI-MS, NMR, and CD spectra. Four compounds(1-4) were successfully isolated from the 80% ethanol extract of the dried fruits, and they were identified as qingqianliulignanoside A(1), cyclopaloside E(2), emodin-8-O-β-D-glucoside(3), and daucosterol(4). Compounds 1 and 2 were new compounds, and compounds 3 was isolated from C. paliurus for the first time. The inhibitory activities of compounds 1-3 against α-glucosidase were assessed via the PNPG method. Compounds 2 exhibited certain α-glucosidase inhibitory activity, with the IC_(50) value of(148.3±11.4) μg·mL~(-1).</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 5","pages":"1367-1372"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Correlation between chemical composition and origin of Notoginseng Radix et Rhizoma based on LC-MS, GC-MS, and electronic nose].","authors":"Liang Li, Ming-Li Li, Hui-Jun Wang, Yu-Heng Wang, Xiao-Lu Wei, Tian Han, Yang Pei, Yan Rong, Hai-Yu Zhao, Li-Hua Chen","doi":"10.19540/j.cnki.cjcmm.20251011.401","DOIUrl":"10.19540/j.cnki.cjcmm.20251011.401","url":null,"abstract":"<p><p>In this study, thirteen batches of Notoginseng Radix et Rhizoma samples from Wenshan and non-Wenshan producing areas in Yunnan, China were selected as the research objects. Untargeted metabolomics analysis was performed via UHPLC-LTQ-Orbitrap-MS and GC-Q-TOF-MS to systematically compare the chemical composition of Notoginseng Radix et Rhizoma from different origins. Meanwhile, electronic nose technology was employed to capture olfactory characteristics, and multivariate statistical analysis was adopted to investigate the correlations between electronic nose responses and chemical markers, thereby comprehensively evaluating the impact of geographical origin on the quality of Notoginseng Radix et Rhizoma from multiple dimensions. The results of untargeted metabolomics showed significant differences in the chemical composition of Notoginseng Radix et Rhizoma between Wenshan and non-Wenshan producing areas. A total of 126 non-volatile and 11 volatile differential components were identified. Among them, 60 non-volatile and 3 volatile components had higher levels in Wenshan-sourced Notoginseng Radix et Rhizoma, primarily including saponins, flavonoids and their glycosides, uracil, and hydroxyproline. In contrast, samples from areas other than Wenshan showed higher levels of esters, small-molecule organic acids, sterols, terpenes, and cyclohexene derivatives. The electronic nose analysis further confirmed significant differences in the odor of Notoginseng Radix et Rhizoma from different origins. The Wenshan-sourced Notoginseng Radix et Rhizoma exhibited stronger responses in the W2W(sensitive to organic sulfurs), W1W(sensitive to inorganic sulfurs), and W5S(sensitive to nitrogen oxides) sensors than the samples from areas other than Wenshan. This study elucidates the influence of geographical origin on the quality of Notoginseng Radix et Rhizoma from both chemical composition(differential compounds of various polarity) and electronic senses and obtains quality markers closely related to the origin. It provides a scientific basis and data support for the rapid and accurate identification of Notoginseng Radix et Rhizoma origins.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 6","pages":"1583-1592"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Therapeutic effects of Baitouweng Decoction on ulcerative colitis mice with dampness-heat syndrome via inhibiting CaSR-Gq/11-MAPK signaling pathway based on fecal microbiota transplantation].","authors":"Xue Zheng, Dan-Dan Ye, Xiao-Ya Weng, Zhuo-Wen Huang, Yi-Hui Li, Lin-Sheng Zhang, Mu-Yuan Chen, Fei-Yang Liu, Ji-Ye Zhu, Ming-Guo Dong, Xue-Bao Zheng, Xiao-Qi Huang","doi":"10.19540/j.cnki.cjcmm.20251210.706","DOIUrl":"10.19540/j.cnki.cjcmm.20251210.706","url":null,"abstract":"&lt;p&gt;&lt;p&gt;This study aimed to investigate whether Baitouweng Decoction(BTD) exerts pharmacodynamic effects on ulcerative colitis(UC) mice with dampness-heat syndrome in the large intestine by modulating the gut microbiota and its metabolites to inhibit the calcium sensing receptor(CaSR)-G-protein alpha subunit Q/G-protein alpha subunit 11(Gq/11)-mitogen activated protein kinase(MAPK) signaling pathway. In the experiment, mice were divided into the following groups: a normal group, a model group, a Baitouweng Decoction fecal microbiota transplantation(BTD-FMT) group, a normal-FMT group, and a normal-FMT + Akkermansia muciniphila group. Except for the normal group, a UC with dampness-heat syndrome model was established in all mice. The corresponding fecal supernatant or Akkermansia muciniphila was administered on days 9, 11, 13, 15, 17, 19, and 21. Items below were recorded: body weight, diet, dampness-heat syndrome modeling conditions, and disease activity index(DAI) scores. The distribution and fluorescence intensity of fluorescein isothiocyanate(FITC)-Dextran in the gut were detected using a small animal 3D live imaging system. Pathological changes in the colon and tongue tissues were observed using hematoxylin-eosin(HE) staining. The mRNA expression of inflammatory factors and Muc2 in colon tissue was measured by quantitative real-time PCR(qRT-PCR). The expression levels of forkhead box protein p3(Foxp3) and retinoic acid related orphan receptor γt(RORγt) in colon tissue were analyzed by immunohistochemistry. Goblet cell and mucin expression were analyzed by alcian blue-periodic acid-Schiff(AB-PAS) staining. Western blot was employed to detect the expression of Zonula occludens-1(ZO-1), Occludin, Claudin-1, CaSR, G-protein alpha subunit αQ(Gnaq), G-protein alpha subunit 11(Gna11), extracellular signal-regulated kinase 1/2(ERK1/2), c-Jun N-terminal kinase(JNK), mitogen-activated protein kinase p38(p38 MAPK, p38), phosphorylated extracellular signal-regulated kinase 1/2(p-ERK1/2), phosphorylated c-Jun N-terminal kinase(p-JNK), and phosphorylated mitogen-activated protein kinase p38(p-p38 MAPK, p-p38) in colon tissue. The results indicated that compared with the normal group, model mice with dampness-heat syndrome showed significant increases in rectal temperature, water intake, DAI scores, total movement trajectories/distance traveled in the open field test, and dampness-heat syndrome scores. Compared with the model group, BTD-FMT exerted therapeutic effects on UC mice with dampness-heat syndrome, which were evidenced by significantly ameliorated dampness-heat symptoms and tongue tissue pathology, reduced DAI scores, restored colon length, decreased colon histopathological scores, and modulated immune responses. Furthermore, BTD-FMT downregulated the mRNA expression of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), and interleukin-6(IL-6) while upregulating interleukin-10(IL-10) and interleukin-4(IL-4) expression. Barrier protein levels were ","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 5","pages":"1259-1272"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanism of Angelicae Pubescentis Radix-Taxilli Herba components in preventing nucleus pulposus cell apoptosis via regulating PINK1/Parkin and Nrf2 pathways based on \"Qu-Zhi-Bu-Yi\" theory].","authors":"Chao-Qun Feng, Min Zhao, Mao-Yin Luo, Chuan Leng, Fei Yang, Xiao-Hong Fan","doi":"10.19540/j.cnki.cjcmm.20251113.706","DOIUrl":"10.19540/j.cnki.cjcmm.20251113.706","url":null,"abstract":"<p><p>Based on the theory of ″Qu-Zhi-Bu-Yi″, this study aimed to investigate the optimal compatibility ratio of Angelicae Pubescentis Radix and Taxilli Herba for preventing and treating pressure-induced human nucleus pulposus(NP) cell apoptosis, and to elucidate the mechanisms. A human NP cell apoptosis model was established using abnormal mechanical pressure at 1.0 MPa. The concentration ratios of quercetin, osthole, and columbianadin were screened by the orthogonal experimental design. Cell proliferation and apoptosis were determined using the cell counting kit-8(CCK-8) assay and Annexin V-APC/PI double staining, respectively. Western blot was performed to detect apoptosis-related proteins, as well as key protein expressions in the phosphatase and tensin homolog(PTEN)-induced putative kinase 1(PINK1)/Parkin-mediated mitophagy pathway, and the nuclear factor erythroid 2-related factor 2(Nrf2)-mediated antioxidant pathway. Mitochondrial ultrastructure was observed by transmission electron microscopy(TEM). Mitochondrial membrane potential was measured using the JC-1 staining. Oxidative stress markers were evaluated by the enzyme-linked immunosorbent assay(ELISA). Energy metabolism was quantified using colorimetry. Reactive oxygen species(ROS) levels were detected using the 2',7'-dichlorodihydrofluorescein diacetate(DCFH-DA) fluorescent probe. The Nrf2 overexpression and knockdown model construction efficacy in NP cells was validated by quantitative real-time polymerase chain reaction(qRT-PCR). Additionally, rescue experiments were designed to validate the mechanism of the above pathways. Based on the results, the optimal combination of quercetin at 50 μmol·L~(-1), osthole at 6.25 μmol·L~(-1), and columbianadin at 12.5 μmol·L~(-1) significantly promoted NP cell proliferation and inhibited apoptosis. The components enhanced mitophagy by activating the PINK1/Parkin pathway and alleviated oxidative stress by activating the Nrf2 pathway. Rescue experiments showed that the anti-apoptotic effects of the traditional Chinese medicine(TCM) components could be reversed by mitophagy inhibition and Nrf2 knockdown. In conclusion, the Angelicae Pubescentis Radix-Taxilli Herba components inhibit pressure-induced NP cell apoptosis by synergistically activating PINK1/Parkin-mediated mitophagy and Nrf2-mediated antioxidant response. These findings provide experimental evidence for intervertebral disc degeneration treatment.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"51 6","pages":"1724-1735"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147730704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}