Plant Communications最新文献_第2页

The chloroplast pentatricopeptide repeat protein RCN22 regulates tiller number in rice by affecting sugar levels via the TB1-RCN22-RbcL module. 叶绿体五肽重复蛋白 RCN22 通过 TB1-RCN22-RbcL 模块影响糖分水平，从而调节水稻的分蘖数量。

IF 9.4 1区生物学

Plant Communications Pub Date : 2024-08-27 DOI: 10.1016/j.xplc.2024.101073

Tianyu Mo, Tianhao Wang, Yinglu Sun, Ashmit Kumar, Humphrey Mkumbwa, Jingjing Fang, Jinfeng Zhao, Shoujiang Yuan, Zichao Li, Xueyong Li

{"title":"The chloroplast pentatricopeptide repeat protein RCN22 regulates tiller number in rice by affecting sugar levels via the TB1-RCN22-RbcL module.","authors":"Tianyu Mo, Tianhao Wang, Yinglu Sun, Ashmit Kumar, Humphrey Mkumbwa, Jingjing Fang, Jinfeng Zhao, Shoujiang Yuan, Zichao Li, Xueyong Li","doi":"10.1016/j.xplc.2024.101073","DOIUrl":"https://doi.org/10.1016/j.xplc.2024.101073","url":null,"abstract":"As an important yield component, the rice tiller number controls panicle number and determines grain yield. The regulation of rice tiller number by chloroplast pentatricopeptide repeat (PPR) proteins has not been reported. Here, we report a rice reduced culm number22 (rcn22) mutant which produces few tillers due to suppressed tiller bud elongation. Map-based cloning revealed that RCN22 encodes a chloroplast-localized P-type PPR protein. We found that RCN22 specifically binds to the 5'-UTR of RbcL mRNA (encoding the large subunit of Rubisco) and enhances its stability. The reduced RbcL mRNA abundance in rcn22 led to a lower photosynthetic rate and decreased sugar levels. Consequently, transcript levels of DWARF3 (D3) and TEOSINTE BRANCHED1 (TB1) (encoding negative regulators of tiller bud elongation) increased, whereas protein levels of a positive regulator DWARF53 (D53) decreased. Furthermore, high concentrations of sucrose could rescue the tiller bud growth defect of the rcn22 mutant. On the other hand, TB1 directly binds to the RCN22 promoter and downregulates its expression. The tb1/rcn22 double mutant showed a tillering phenotype similar to rcn22. Our results suggest that the TB1-RCN22-RbcL module plays a vital role in rice tiller bud elongation by affecting sugar levels.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine-tuning of the dual-role transcription factor WRKY8 via differential phosphorylation for robust broad-spectrum plant immunity. 通过不同的磷酸化微调双重作用转录因子 WRKY8，实现强大的广谱植物免疫。

IF 9.4 1区生物学

Plant Communications Pub Date : 2024-08-26 DOI: 10.1016/j.xplc.2024.101072

Chun-Xiu Ren, Song-Yu Chen, Yu-Han He, You-Ping Xu, Juan Yang, Xin-Zhong Cai

{"title":"Fine-tuning of the dual-role transcription factor WRKY8 via differential phosphorylation for robust broad-spectrum plant immunity.","authors":"Chun-Xiu Ren, Song-Yu Chen, Yu-Han He, You-Ping Xu, Juan Yang, Xin-Zhong Cai","doi":"10.1016/j.xplc.2024.101072","DOIUrl":"https://doi.org/10.1016/j.xplc.2024.101072","url":null,"abstract":"Plants utilize plasma membrane-localized pattern recognition receptors (PRRs) to perceive pathogen-associated molecular patterns (PAMPs) to activate broad-spectrum pattern-triggered immunity (PTI). However, the regulatory mechanism ensuring robust broad-spectrum plant immunity remains largely unknown. Here, we reveal the dual roles of the transcription factor WRKY8 in transcriptional regulation of PRR genes: repressing the nlp20/nlp24 receptor gene RLP23 whereas promoting the chitin receptor gene CERK1. Remarkably, SsNLP1 and SsNLP2, two nlp24 type PAMPs in the destructive fungal pathogen Sclerotinia sclerotiorum, activate two calcium-elicited kinases, CPK4 and CPK11 to phosphorylate WRKY8 and consequently release its inhibition on RLP23 expression to accumulate RLP23. Meanwhile, SsNLPs activate a RLCK type kinase, PBL19 to phosphorylate WRKY8 and consequently enhance the accumulation of CERK1. Intriguingly, RLP23 is repressed at late stage by PBL19-mediated phosphorylation of WRKY8, to avoid excessive immunity for normal growth. Our findings unveil a \"killing two birds with one stone\" strategy employed by plants to elicit robust broad-spectrum immunity, which is based on PAMP-triggered fine-tuning of a dual-role transcription factor to simultaneously amplify two PRRs recognizing PAMPs well conserved in a wide range of pathogens. Moreover, our results reveal a novel plant strategy based on fine-tuning of multiple PRR gene expression to balance the trade-off between growth and immunity.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SynDiv: An efficient tool for chromosome collinearity-based population genomics analyses. SynDiv：基于染色体共线性的群体基因组学分析的高效工具。

IF 9.4 1区生物学

Plant Communications Pub Date : 2024-08-23 DOI: 10.1016/j.xplc.2024.101071

Ze-Zhen Du, Jia-Bao He, Wen-Biao Jiao

引用次数: 0

Large-scale production of rice haploids by combining superior haploid inducer with PTGMS lines. 通过将优良单倍体诱导剂与 PTGMS 株系相结合，大规模生产水稻单倍体。

IF 9.4 1区生物学

Plant Communications Pub Date : 2024-08-22 DOI: 10.1016/j.xplc.2024.101067

Chaolei Liu, Song Yan, Fangming Mao, Tingting Sun, Huan Liang, Qing Liu, Qian Qian, Kejian Wang

引用次数: 0

GENOMES UNCOUPLED PROTEIN1 binds to plastid RNAs and promotes their maturation. GENOMES UNCOUPLED PROTEIN1 与质体 RNA 结合并促进其成熟。

IF 9.4 1区生物学

Plant Communications Pub Date : 2024-08-21 DOI: 10.1016/j.xplc.2024.101069

Qian Tang, Duorong Xu, Benjamin Lenzen, Andreas Brachmann, Madhura M Yapa, Paymon Doroodian, Christian Schmitz-Linneweber, Tatsuru Masuda, Zhihua Hua, Dario Leister, Tatjana Kleine

{"title":"GENOMES UNCOUPLED PROTEIN1 binds to plastid RNAs and promotes their maturation.","authors":"Qian Tang, Duorong Xu, Benjamin Lenzen, Andreas Brachmann, Madhura M Yapa, Paymon Doroodian, Christian Schmitz-Linneweber, Tatsuru Masuda, Zhihua Hua, Dario Leister, Tatjana Kleine","doi":"10.1016/j.xplc.2024.101069","DOIUrl":"https://doi.org/10.1016/j.xplc.2024.101069","url":null,"abstract":"Plastid biogenesis and the coordination of plastid and nuclear genome expression through anterograde and retrograde signaling are essential for plant development. GENOMES UNCOUPLED1 (GUN1) plays a central role in retrograde signaling during early plant development. The putative function of GUN1 has been extensively studied, but its molecular function remains controversial. Here, we evaluate published transcriptome data and generate our own data from gun1 mutants grown under signaling relevant conditions to show that editing and splicing are not relevant for GUN1-dependent retrograde signaling. Our study of the plastid (post)-transcriptome of gun1 seedlings with white and pale cotyledons demonstrates that GUN1 deficiency significantly alters the entire plastid transcriptome. By combining this result with a PPR code-based prediction and experimental validation by RNA immunoprecipitation experiments, several putative targets of GUN1 were identified, including tRNAs and RNAs derived from ycf1.2, rpoC1 and rpoC2, and the ndhH-ndhA-ndhI-ndhG-ndhE-psaC-ndhD gene cluster. The absence of plastid rRNAs and the significant reduction of almost all plastid transcripts in white gun1 mutants account for the cotyledon phenotype. Our study provides evidence for RNA binding and maturation as the long-sought molecular function of GUN1 and resolves long-standing controversies. We anticipate that our findings will serve as a basis for subsequent studies investigating the mechanism of plastid gene expression and will facilitate the elucidation of GUN1's function in retrograde signaling.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated pipeline facilitates fast cloning of a new powdery mildew resistance gene from the wheat wild relative Aegilops umbellulata. 集成管道有助于快速克隆小麦野生近缘种 Aegilops umbellulata 的抗白粉病新基因。

IF 9.4 1区生物学

Plant Communications Pub Date : 2024-08-21 DOI: 10.1016/j.xplc.2024.101070

Huagang He, Jiale Wang, Jiabao Liang, Qianyuan Zhang, Minfeng Xue, Zhaozhao Chen, Qiulian Tang, Xiaobei Chen, Shanying Zhu, Yajun Wang

引用次数: 0

IsDge10 is a hypercompact TnpB nuclease that confers efficient genome editing in rice. IsDge10 是一种超小型 TnpB 核酸酶，它能在水稻中实现高效的基因组编辑。

IF 9.4 1区生物学

Plant Communications Pub Date : 2024-08-20 DOI: 10.1016/j.xplc.2024.101068

Rui Zhang, Xu Tang, Yao He, Yangcun Li, Wei Wang, Yawei Wang, Danning Wang, Xuelian Zheng, Yiping Qi, Yong Zhang

引用次数: 0

Landrace introgression contributed to the recent feralization of weedy rice in East China. 陆稻引种导致了华东地区杂交水稻的近代野化。

IF 9.4 1区生物学

Plant Communications Pub Date : 2024-08-20 DOI: 10.1016/j.xplc.2024.101066

Min Zhu, Kaicheng Yong, Kai Xu, Jia Cong, Xiaofang Zhou, Keyue Liu, Xuechen Wang, Longjiang Fan, Kenneth M Olsen, Xuehui Huang, Xiaoyi Zhou, Jie Qiu

引用次数: 0

An abscisic acid-responsive transcriptional regulatory module CsERF110-CsERF53 orchestrates citrus fruit coloration. 脱落酸响应转录调控模块 CsERF110-CsERF53 协调柑橘果实着色。

IF 9.4 1区生物学

Plant Communications Pub Date : 2024-08-20 DOI: 10.1016/j.xplc.2024.101065

Quan Sun, Zhengchen He, Di Feng, Ranran Wei, Yingzi Zhang, Junli Ye, Lijun Chai, Juan Xu, Yunjiang Cheng, Qiang Xu, Xiuxin Deng

{"title":"An abscisic acid-responsive transcriptional regulatory module CsERF110-CsERF53 orchestrates citrus fruit coloration.","authors":"Quan Sun, Zhengchen He, Di Feng, Ranran Wei, Yingzi Zhang, Junli Ye, Lijun Chai, Juan Xu, Yunjiang Cheng, Qiang Xu, Xiuxin Deng","doi":"10.1016/j.xplc.2024.101065","DOIUrl":"https://doi.org/10.1016/j.xplc.2024.101065","url":null,"abstract":"Carotenoid biosynthesis is closely associated with abscisic acid (ABA) during the ripening process of non-climacteric fruits, but the regulatory mechanism between ABA signaling and carotenoid metabolism remains largely unclear. Here, we identified two master regulators of ABA-mediated citrus fruit coloration, CsERF110 and CsERF53, which activated the expression of carotenoid metabolism genes (CsGGPPS, CsPSY, CsPDS, CsCRTISO, CsLCYB2, CsLCYE, CsHYD, CsZEP, and CsNCED2) to facilitate carotenoid accumulation. Further investigations showed that CsERF110 not only activated the expression of CsERF53 by binding to its promoter, but also interacted with CsERF53 to form a transcriptional regulatory module CsERF110-CsERF53. Furthermore, we discovered a positive feedback regulation loop between the ABA signal and carotenoid metabolism regulated by the transcriptional regulatory module CsERF110-CsERF53. Our results reveal that the transcriptional regulatory module CsERF110-CsERF53 responded to ABA signaling, thereby orchestrating citrus fruit coloration. Considering the importance of carotenoid content for citrus and many other carotenoid-rich crops, the revelation of molecular mechanisms underlying ABA-mediated carotenoid biosynthesis in plants will facilitate transgenic/gene editing approach development, further contributing to improving the quality of citrus and other carotenoid-rich crops.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142009891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct RNA sequencing in plants: practical applications and future perspectives. 植物中的直接 RNA 测序：实际应用与未来展望。

IF 9.4 1区生物学

Plant Communications Pub Date : 2024-08-18 DOI: 10.1016/j.xplc.2024.101064

Xi-Tong Zhu, Pablo Sanz-Jimenez, Xiao-Tong Ning, Muhammad Tahir Ul Qamar, Ling-Ling Chen

{"title":"Direct RNA sequencing in plants: practical applications and future perspectives.","authors":"Xi-Tong Zhu, Pablo Sanz-Jimenez, Xiao-Tong Ning, Muhammad Tahir Ul Qamar, Ling-Ling Chen","doi":"10.1016/j.xplc.2024.101064","DOIUrl":"https://doi.org/10.1016/j.xplc.2024.101064","url":null,"abstract":"The transcriptome serves as a bridge that links genomic variation and phenotype diversity. A vast number of studies using next-generation RNA sequencing (RNA-seq) in the last two decades emphasize the essential roles of plant transcriptome in response to developmental and environmental conditions, leading to numerous insights into the dynamic change, evolutionary trace and elaborate regulation of plant transcriptome. With substantial improvement in accuracy and throughput, direct RNA sequencing (DRS) has emerged as a new and powerful sequencing platform for the precise detection of native and full-length transcripts, which overcomes many limitations such as read length and PCR bias that are inherent to short-read RNA-seq. Here, we reviewed recent advances in dissecting the complexity and diversity of plant transcriptome utilizing DRS as a main technological mean from many aspects of RNA metabolism, including novel isoforms, poly(A) tail and RNA modification, and proposed a comprehensive workflow for the data process of plants DRS. Many challenges concerning the application of DRS in plants, such as machine learning tools tailored to plant transcriptome, remain to be solved, and together we prospect the future biological questions that can be potentially answered by DRS such as allele-specific RNA modification. This technology provides convenient support on which the connection of distinct RNA features is tightly built, sustainably refining our understanding of the biological functions of plant transcriptome.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":null,"pages":null},"PeriodicalIF":9.4,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142001308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0