Plant Communications最新文献_第2页

DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains. DeepTYLCV：一种可解释且经实验验证的预测番茄黄卷叶病毒不同毒株毒力的人工智能模型。

IF 11.6 1区生物学

Plant Communications Pub Date : 2026-04-29 DOI: 10.1016/j.xplc.2026.101877

Nattanong Bupi, Hariharan Sangaraju, Duong Thanh Tran, Vinoth Kumar Sangaraju, Hyojin Im, Minkwan Kim, Sukchan Lee, Balachandran Manavalan

{"title":"DeepTYLCV: An interpretable and experimentally validated AI model for predicting virulence of different tomato yellow leaf curl virus strains.","authors":"Nattanong Bupi, Hariharan Sangaraju, Duong Thanh Tran, Vinoth Kumar Sangaraju, Hyojin Im, Minkwan Kim, Sukchan Lee, Balachandran Manavalan","doi":"10.1016/j.xplc.2026.101877","DOIUrl":"https://doi.org/10.1016/j.xplc.2026.101877","url":null,"abstract":"Tomato yellow leaf curl virus (TYLCV) is among the most devastating pathogens affecting tomato production worldwide, with emerging virulent strains increasingly overcoming genetic resistance and triggering severe outbreaks. Traditional field diagnosis, reliant on visual inspection or image-based AI models, remains constrained by symptom dependence, environmental variability, and poor strain-level interpretability. To address these challenges, we introduce DeepTYLCV, a novel deep learning framework for accurate virulence prediction directly from viral genome-derived open reading frame (ORF) sequences. We first constructed a comprehensive dataset of globally sourced TYLCV sequences, curated by virulence annotations. DeepTYLCV integrates protein language model (PLM)-based embeddings with optimal concatenated conventional descriptors (optCCDs) using a hybrid architecture composed of a Transformer encoder and a multi-scale convolutional neural network (CNN), enabling effective extraction of both global and local sequence features. Benchmark analyses demonstrate that DeepTYLCV significantly outperforms our previously developed IML-TYLCV model, which was trained on Korean isolates and lacked global generalizability. Importantly, blind predictions on 15 uncharacterized or representative TYLCV isolates were experimentally validated in tomato plants, achieving 100% concordance between model predictions and observed symptom severity. Furthermore, 1D-Grad-CAM++-based interpretability analyses revealed that the model consistently focused on relevant sequence motifs associated with severe strains, offering mechanistic insights into symptom severity. DeepTYLCV is publicly available at https://balalab-skku.org/DeepTYLCV/, represents a powerful, interpretable, and globally scalable platform for early TYLCV surveillance, resistance monitoring, and strategic disease management in tomato cultivation.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101877"},"PeriodicalIF":11.6,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DockingDB: an online reverse docking database for plant hormone research. DockingDB：用于植物激素研究的在线反向对接数据库。

IF 11.6 1区生物学

Plant Communications Pub Date : 2026-04-29 DOI: 10.1016/j.xplc.2026.101880

Yi-Xiong Guo, Fenmei Liu, Xin-Dong Xu, Guolyu Xu, Si-Ying Ye, Yan-Han Shao, Yanqiong Kong, Zihui Su, Qiaoqiao Zhao, Qiong Fu, Jinxue Ma, Jia-Ming Song, Wenguo Cai, Zhenhua Ming, Ling-Ling Chen

引用次数: 0

The dehydrin protein COR15 enhances antiviral RNA silencing by preventing viral protein-mediated SGS3 degradation and promoting SGS3 condensate formation. 脱氢蛋白COR15通过阻止病毒蛋白介导的SGS3降解和促进SGS3凝聚物形成来增强抗病毒RNA沉默。

IF 11.6 1区生物学

Plant Communications Pub Date : 2026-04-29 DOI: 10.1016/j.xplc.2026.101879

Yong-Le Zhang, Zuo-Kun Yang, Zhe Zhang, Li-Yuan Ma, Shan-Shan Jiang, Yan-Ping Tian, Guo-Ping Wang, Ni Hong, Xiang-Dong Li

{"title":"The dehydrin protein COR15 enhances antiviral RNA silencing by preventing viral protein-mediated SGS3 degradation and promoting SGS3 condensate formation.","authors":"Yong-Le Zhang, Zuo-Kun Yang, Zhe Zhang, Li-Yuan Ma, Shan-Shan Jiang, Yan-Ping Tian, Guo-Ping Wang, Ni Hong, Xiang-Dong Li","doi":"10.1016/j.xplc.2026.101879","DOIUrl":"https://doi.org/10.1016/j.xplc.2026.101879","url":null,"abstract":"RNA silencing, a conserved gene regulatory mechanism mediated by small interfering RNAs (siRNAs), is a major component of plant antiviral immunity. The plant RNA-binding protein SUPPRESSOR OF GENE SILENCING 3 (SGS3) forms condensates that drive siRNA body assembly and promote siRNA biogenesis, but the regulators of SGS3 condensate formation and their roles in antiviral responses remain largely unknown. Here, we show that the dehydrin protein COR15 from Citrus aurantifolia and Nicotiana benthamiana interacts with citrus tristeza virus (CTV)-encoded p20 protein. COR15 abolishes the RNA silencing suppressor activity of p20, positively regulates antiviral RNA silencing, and confers resistance to CTV in N. benthamiana plants. Furthermore, COR15 also restricts the infection of other positive-sense RNA viruses, suggesting that COR15 functions as a broad-spectrum antiviral factor. Mechanistically, CTV infection induces NbCOR15 expression and promotes COR15 granule formation in the cytoplasm. COR15 attenuates p20-mediated SGS3 degradation by disrupting the p20-SGS3 interaction, thereby stabilizing SGS3. COR15 is recruited into siRNA bodies and undergoes liquid-liquid phase separation within these bodies. Notably, COR15 promotes SGS3 condensates formation and efficient siRNA processing. Knockout of COR15 via CRISPR/Cas9 in N. benthamiana plants reduces SGS3 condensate number, causes abnormally sized SGS3 condensates, and impairs SGS3-dependent siRNA synthesis, indicating that COR15 is a previously unrecognized component of the siRNA bodies. Together, this work reveals the function and mechanism of a dehydrin protein in plant antiviral immunity and provides new sight into the molecular arms race during plant-virus co-evolution.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101879"},"PeriodicalIF":11.6,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoupling the 'Attract-and-Kill' Strategy: Independent Functions for Zoospore Attraction and ROS-Executed Killing Synergize in Disease-Suppressive Intercropping. “吸引-杀死”策略的解耦：在抗病性间作中游动孢子吸引和ros杀伤协同作用的独立函数。

IF 11.6 1区生物学

Plant Communications Pub Date : 2026-04-29 DOI: 10.1016/j.xplc.2026.101876

Yixiang Liu, Jiaqing Wu, Yuxin Yang, Yiyi He, Rong Zhou, Ying Li, Junwei Sun, Mingchun Gong, Xinyue Mei, Yingbin Li, Huichuan Huang, Fei Du, Weiping Deng, Chen Ye, Xiahong He, Long Li, Jianjun Hao, Min Yang, Youyong Zhu, Shusheng Zhu

{"title":"Decoupling the 'Attract-and-Kill' Strategy: Independent Functions for Zoospore Attraction and ROS-Executed Killing Synergize in Disease-Suppressive Intercropping.","authors":"Yixiang Liu, Jiaqing Wu, Yuxin Yang, Yiyi He, Rong Zhou, Ying Li, Junwei Sun, Mingchun Gong, Xinyue Mei, Yingbin Li, Huichuan Huang, Fei Du, Weiping Deng, Chen Ye, Xiahong He, Long Li, Jianjun Hao, Min Yang, Youyong Zhu, Shusheng Zhu","doi":"10.1016/j.xplc.2026.101876","DOIUrl":"https://doi.org/10.1016/j.xplc.2026.101876","url":null,"abstract":"Soilborne Phytophthora diseases pose a major threat to agricultural sustainability. How non-host roots disrupt the transmission of soilborne Phytophthora pathogens without relying on classical antimicrobial exudates remains unknown. Through a decade-long field study, we demonstrate that strip intercropping achieves sustainable disease suppression (up to 46.85%) by leveraging non-host roots as ecological barriers that intercept zoospore transmission. Moving beyond the conventional focus on antimicrobial exudates, we decouple the 'attract-and-kill' strategy into two discrete functions: a broad-spectrum attraction function widespread among non-host plants (13 of 15 genera), which alone reduces disease by 9.2-24.4%; and a specialized killing function restricted to few species (e.g., garlic), where elevated root-interface concentrations of sulfur compounds induce cystospore rupture and germination inhibition, delivering 42.9-49.3% field suppression. The synergy of universal attraction and targeted killing intensifies efficacy at the rhizosphere interface. Mechanistically, killing is executed through a conserved ROS-PCD pathway, with pathogen sensitivity determined by intrinsic redox-buffering capacity. Metagenomic profiling further revealed that garlic roots and sulfur compounds enrich microbial motility genes and apoptosis pathways, adding a complementary mechanistic layer to the 'attract and kill'framework. We thus propose this two-component, ecology-based strategy for sustainable Phytophthora management in diversified cropping systems.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101876"},"PeriodicalIF":11.6,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147823374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multiplex hextuple luciferase assay for advancing cellular signaling analysis in plant cells. 一种用于推进植物细胞信号分析的多重六元荧光素酶试验。

IF 11.6 1区生物学

Plant Communications Pub Date : 2026-04-25 DOI: 10.1016/j.xplc.2026.101870

Shanshan Song, Saloni Darji, Nathaniel Carter, Koen J T Venken, Santiago Navarro

{"title":"A multiplex hextuple luciferase assay for advancing cellular signaling analysis in plant cells.","authors":"Shanshan Song, Saloni Darji, Nathaniel Carter, Koen J T Venken, Santiago Navarro","doi":"10.1016/j.xplc.2026.101870","DOIUrl":"https://doi.org/10.1016/j.xplc.2026.101870","url":null,"abstract":"Plant cell-based assays offer substantial cost and throughput advantages over in planta testing. Current protoplast-based cellular activity assays are single biological measurements that require rigorous control to normalize inherent experimental variations when comparing across experiments. Commonly used assays incorporate two colorimetric, fluorescent, or luminescent probes, or a combination thereof, to measure one cellular response normalized against a control. Recently, multiplex hextuple luciferase assaying was developed to interrogate five signaling pathways against a control at once in mammalian cells. Inspired by this work, we explored, optimized, and adopted this strategy for plant protoplast applications, enabling the simultaneous measurement of up to five different cellular activities against a control from a single plant sample. As a proof of principle, we engineered inducible promoter-driven luciferase reporters to observe five different promoter activities activated by the exogenous application of stimuli against a constitutive control luciferase reporter. The strength of exogenous inducers, small-molecule inhibitors, or genetic small interfering RNA is indicated by luminescence light emission from the expression of luciferase driven by the different reporter activities. We measured inducible promoter activities by simultaneously reading multiple luciferases per sample, which significantly reduced the sample size and improved across experimental comparisons. The results of inducible promoter activation and inhibition using multiplexed luciferase reporters were comparable to those of conventional single luciferase reporters. The protoplast-based multiplex luciferase assay multiplies the number of testing agents in a single experiment by five, improving detection sensitivity through light emission measurements and enhancing comparability across experiments.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101870"},"PeriodicalIF":11.6,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147789020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and hormonal regulation of plant responses to waterlogging stress: From fundamental mechanisms to potential strategies of crop tolerance engineering. 植物对涝渍胁迫反应的分子和激素调控：从基本机制到作物耐受性工程的潜在策略。

IF 11.6 1区生物学

Plant Communications Pub Date : 2026-04-25 DOI: 10.1016/j.xplc.2026.101875

Zhengyuan Xu, Zheng Wang, Nanfei Jin, Yuling Zheng, Qiufang Shen, Lingzhen Ye, Guoping Zhang

{"title":"Molecular and hormonal regulation of plant responses to waterlogging stress: From fundamental mechanisms to potential strategies of crop tolerance engineering.","authors":"Zhengyuan Xu, Zheng Wang, Nanfei Jin, Yuling Zheng, Qiufang Shen, Lingzhen Ye, Guoping Zhang","doi":"10.1016/j.xplc.2026.101875","DOIUrl":"https://doi.org/10.1016/j.xplc.2026.101875","url":null,"abstract":"Waterlogging is a major environmental constraint that severely restricts plant growth by causing oxygen deprivation and metabolic disruption in root zones. In response, plants activate sophisticated physiological and molecular mechanisms mediated by phytohormones under hypoxia. Although key hormones, including ethylene (ET), auxin (IAA), gibberellin (GA), abscisic acid (ABA), cytokinin (CK), jasmonic acid (JA), salicylic acid (SA), and brassinosteroid (BR), are known to play central roles in regulating waterlogging adaptation, a holistic understanding of how their intricate crosstalk orchestrates adaptive decisions remains fragmented. In this review, we synthesize recent advances to construct an integrated framework that spans initial oxygen sensing and hormone-driven morphological adaptations to critical metabolic and physiological adjustments under waterlogging. Particularly, we introduce the novel concept 'stress-metabolic integration and hormonal allocation' under waterlogging/hypoxia, which explains how redox, carbon, and hormones are quantitatively integrated to determine cell fate. We also highlight how synergistic and antagonistic interactions among major hormones fine-tune the balance between survival strategies and growth repression. Furthermore, we integrate hormonal treatments with practical and effective agronomic strategies to enhance crop waterlogging tolerance under field conditions. Beyond synthesis, we identify critical knowledge gaps and propose transformative research directions, offering a blueprint for the rational design of 'climate-resilient crops' through targeted manipulation of molecular and hormonal networks in practical agriculture.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101875"},"PeriodicalIF":11.6,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147789031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The OsGSK1-OsBZR3-OsJAZ4 module integrates nitrate signaling with brassinosteroid and jasmonic acid crosstalk to drive cell wall remodeling for salt recovery in rice. OsGSK1-OsBZR3-OsJAZ4模块将硝酸盐信号与油菜素内酯和茉莉酸串音集成在一起，驱动水稻细胞壁重塑，实现盐的恢复。

IF 11.6 1区生物学

Plant Communications Pub Date : 2026-04-22 DOI: 10.1016/j.xplc.2026.101874

Huijing Yang, Kunyang Song, Hang Yu, Xinxin Zhu, Yating Guo, Zhiyuan Pan, Baoming Guo, Weiye Tu, Ruichen Wang, Zixian Li, Yuping Hu, Nora Gigli-Bisceglia, Hai Zhou, Jian Sun, Chengcai Chu, Xiangdong Liu, Yanxia Zhang

{"title":"The OsGSK1-OsBZR3-OsJAZ4 module integrates nitrate signaling with brassinosteroid and jasmonic acid crosstalk to drive cell wall remodeling for salt recovery in rice.","authors":"Huijing Yang, Kunyang Song, Hang Yu, Xinxin Zhu, Yating Guo, Zhiyuan Pan, Baoming Guo, Weiye Tu, Ruichen Wang, Zixian Li, Yuping Hu, Nora Gigli-Bisceglia, Hai Zhou, Jian Sun, Chengcai Chu, Xiangdong Liu, Yanxia Zhang","doi":"10.1016/j.xplc.2026.101874","DOIUrl":"https://doi.org/10.1016/j.xplc.2026.101874","url":null,"abstract":"Salinity stress severely limits rice productivity. Understanding how crops sustain growth during prolonged salt stress exposure is therefore of critical importance. Here, we demonstrate that a brassinosteroid (BR)-jasmonic acid (JA) signaling network integrates nitrate signals to remodel root cell walls and promote salt recovery in rice (Oryza sativa). The GSK3-like kinase OsGSK1 interacts with and phosphorylates the transcription factor BRASSINAZOLE-RESISTANT3 (OsBZR3), which negatively regulates root growth recovery and seedling survival under prolonged salt stress. OsBZR3 fine-tunes root cell wall thickening and compositional remodeling, as well as the nitrate response in rice under salt stress. Notably, high nitrate supply represses the salt stress-induced phosphorylation of OsBZR3 by OsGSK1. OsBZR3 acts upstream of the JA signaling repressor Jasmonate ZIM-domain (JAZ) 4 (OsJAZ4) by directly binding to its promoter region. The OsGSK1-OsBZR3 module regulates OsJAZ4 activity to orchestrate the expression of downstream cell wall remodeling genes under salt stress. In short, our findings establish the OsGSK1-OsBZR3-OsJAZ4 module as a potential hub integrating nitrate and hormonal signals under prolonged salt stress, identifying novel key genetic targets for breeding salt-resilient crops and advancing sustainable green agricultural practices.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101874"},"PeriodicalIF":11.6,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147789039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MBD4 chromatin reader regulates transcription related to temperature and flowering responses through recognition of histone arginine methylation in Arabidopsis thaliana and Brassica napus. MBD4染色质读取器通过识别拟南芥和甘蓝型油菜的组蛋白精氨酸甲基化来调节与温度和开花反应相关的转录。

IF 11.6 1区生物学

Plant Communications Pub Date : 2026-04-21 DOI: 10.1016/j.xplc.2026.101869

José A Abelenda, Gerardo Carrera-Castaño, Julián Calleja, Iván Del Olmo, Jenifer Pozas, Elisa Alonso-Pérez, Manuel Piñeiro, José A Jarillo

{"title":"The MBD4 chromatin reader regulates transcription related to temperature and flowering responses through recognition of histone arginine methylation in Arabidopsis thaliana and Brassica napus.","authors":"José A Abelenda, Gerardo Carrera-Castaño, Julián Calleja, Iván Del Olmo, Jenifer Pozas, Elisa Alonso-Pérez, Manuel Piñeiro, José A Jarillo","doi":"10.1016/j.xplc.2026.101869","DOIUrl":"https://doi.org/10.1016/j.xplc.2026.101869","url":null,"abstract":"Precise regulation of flowering time in plants is achieved through the tight transcriptional control of master regulators, fine-tuned by the dynamics of histone modifications and structural chromatin alterations. In our search for flowering-related gene networks, we identified METHYL-BINDING DOMAIN PROTEIN 4 (MBD4) as a crucial hub for flowering and high ambient temperature responses in Brassica napus and Arabidopsis. While previously described as a DNA methylation reader, our findings reveal that MBD4 recognises histone arginine methylation. MBD4 genome-wide distribution and coordinated expression with histone methylation writers match arginine methylation global patterns. Although histone lysine methylation is well understood, the role of arginine methylation remains largely unexplored. Our results demonstrate that MBD4 plays a critical role in the transcriptional control of flowering regulators by recognising arginine methylation and facilitating acetylation erasure. Additionally, we confirmed the significance of the Brassica napus MBD4 homeolog in modulating the reproductive phase switch through chromatin alterations at the BnaFLC and BnaMAF4 loci, suggesting that chromatin readers may have a global influence on important developmental traits in crops. Overall, understanding these molecular mechanisms is extremely relevant regarding strategies that improve crop adaptation to global warming.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101869"},"PeriodicalIF":11.6,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147789000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

funPlantGenes: A pan-species curated knowledgebase that bridges crop genomics and improvement. funPlantGenes：一个泛物种管理知识库，连接作物基因组学和改进。

IF 11.6 1区生物学

Plant Communications Pub Date : 2026-04-18 DOI: 10.1016/j.xplc.2026.101872

Jinbiao Cao, Zhengfu Zhou, Ying Yu, Jialei Li, Yating Wang, Yang Li, Lihua Jia, Yidan Ouyang, Wen Yao

引用次数: 0

SnRK2.6-mediated phosphorylation stabilizes Proteasome regulator 1 (PTRE1) to enhance 26S proteasome activity and coordinate ABA signaling in Arabidopsis. snrk2.6介导的磷酸化稳定蛋白酶体调节因子1 (PTRE1)，增强26S蛋白酶体活性，协调ABA信号传导。

IF 11.6 1区生物学

Plant Communications Pub Date : 2026-04-18 DOI: 10.1016/j.xplc.2026.101873

Peng-Chao Hao, Ling-Li Zheng, Jin-Shuang Dou, Hong-Wei Xue

{"title":"SnRK2.6-mediated phosphorylation stabilizes Proteasome regulator 1 (PTRE1) to enhance 26S proteasome activity and coordinate ABA signaling in Arabidopsis.","authors":"Peng-Chao Hao, Ling-Li Zheng, Jin-Shuang Dou, Hong-Wei Xue","doi":"10.1016/j.xplc.2026.101873","DOIUrl":"https://doi.org/10.1016/j.xplc.2026.101873","url":null,"abstract":"Abscisic acid (ABA) regulates diverse aspects of plant growth, particularly adaptive responses to abiotic stress. While the ubiquitin-proteasome system (UPS) is recognized as a pivotal pathway for degrading key ABA signaling components, the regulatory mechanisms governing 26S proteasome activity in ABA-mediated responses remain unresolved. Here, we demonstrate that Arabidopsis Proteasome Regulator 1 (PTRE1) modulates ABA signaling through regulating proteasome activity. Phenotypic analyses revealed the heightened ABA sensitivity of ptre1 mutant. Mechanistically, ABA stabilizes PTRE1 protein to enhance 26S proteasome activity. Sucrose nonfermenting1-related kinase 2.6 (SnRK2.6), the key kinase activated by ABA, directly interacts with and phosphorylates PTRE1, which is essential for PTRE1 stability and function in ABA responses. Interestingly, PTRE1 deficiency elevates ABA-Insensitive 5 protein levels, and ptre1 partially rescues the ABA insensitivity of abi5. Collectively, these findings establish a SnRK2.6-PTRE1 axis that fine-tunes proteasome activity, providing new insights into the coordinated regulation of ABA signaling through dual phosphorylation by SnRK2.6.","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101873"},"PeriodicalIF":11.6,"publicationDate":"2026-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147718749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0