Plant Communications最新文献

{"title":"Transgenic expression of mAChR-C dsRNA in maize confers efficient locust control.","authors":"Hongyuan Zheng, Mengke Hua, Mina Jiang, Chunran Jiang, Yuxi Xi, Jingcai Deng, Huijing Xu, Baojuan Zeng, Shutang Zhou","doi":"10.1016/j.xplc.2025.101316","DOIUrl":"https://doi.org/10.1016/j.xplc.2025.101316","url":null,"abstract":"<p><p>Plant-meditated RNA interference (RNAi), by which double-stranded RNAs (dsRNAs) targeting insect genes are expressed in plants for insect ingestion, has shown great potential for herbivorous insect pest control. Locusts which are among the most destructive agriculture insect pests appear to be resistant to orally delivered naked dsRNA. Moreover, the feasibility of plant-mediated RNAi in suppressing the expression of target genes in locusts remains poorly understood. Using the migratory locust Locusta migratoria, we report here that C-type muscarinic acetylcholine receptor (mAChR-C), a G protein-coupled receptor (GPCR) belonging to bioamine receptor subfamily, played a pivotal role in chitin metabolism by regulating genes responsible for chitin synthesis and degradation. Knockdown of locust mAChR-C by injection-delivered dsRNA caused defective nymph molting and metamorphosis, accompanied by malformation, arrested development and motility. Notably, locusts feeding on transgenic maize expressing locust mAChR-C dsRNAs exhibited defective phenotypes similar to those subjected to injection of mAChR-C dsRNA. However, ingestion of transgenic maize with locust mAChR-C dsRNA had no significant effect on non-target insects including the fall armyworm Spodoptera frugiperda, the cotton bollworm Helicoverpa armigera, the Asian corn borer Ostrinia furnacalis and the oriental armyworm Mythimna separata. Our results suggest that transgene expression of locust mAChR-C dsRNA is an efficient RNAi approach for locusts, which offers a promising eco-friendly strategy for locust management.</p>","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101316"},"PeriodicalIF":9.4,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a key peptide cyclase for novel cyclic peptide discovery in Pseudostellaria heterophylla.","authors":"Xianjin Qin, Fengjiao Wang, Dejin Xie, Qi Zhou, Sheng Lin, Wenxiong Lin, Wei Li","doi":"10.1016/j.xplc.2025.101315","DOIUrl":"https://doi.org/10.1016/j.xplc.2025.101315","url":null,"abstract":"<p><p>Orbitides, also known as Caryophyllaceae-type cyclic peptides, from Traditional Chinese Medicine plant Pseudostellaria heterophylla (Mil.) Pax have great potential for improving memory and treating diabetes. Orbitides are ribosomally encoded and post-translationally modified peptides, but this key biosynthesis enzyme is still unknown in P. heterophylla. We investigated the orbitide distribution in P. heterophylla and mined novel precursor peptide genes and peptide cyclase from multiple omics data. The function of the key tailoring gene was elucidated using transient heterologous expression and virus-induced gene silencing systems. Our findings suggest that PhPCY3 is a unique gene involved in the cyclization of linear precursor peptides in planta. Molecular docking and multiple sequence alignment, followed by site-directed mutagenesis, showed that N500 and S502 were the key amino acid residues. More than 100 precursor peptide gene sequences were identified, and known active orbitides, such as heterophyllin B and pseudostellarin E/F/G, were successfully biosynthesized. Four novel orbitides, namely cyclo-[LDGPPPYF], cyclo-[WGSSTPHT], cyclo-[GLPIGAPWG], and cyclo-[FGDVGPVI], were identified using the heterologous expression platform. In this study, we describe a gene-guided approach for elucidating the biosynthesis pathway and discovering novel orbitides. Our work provides a strategy for mining and biosynthesizing novel orbitides in P. heterophylla and other plants to further investigate their activities.</p>","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101315"},"PeriodicalIF":9.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein phosphorylation and oxidative protein modification promote plant photosystem II disassembly for repair.","authors":"Steven D McKenzie, Sujith Puthiyaveetil","doi":"10.1016/j.xplc.2024.101202","DOIUrl":"10.1016/j.xplc.2024.101202","url":null,"abstract":"<p><p>The light-driven water-splitting reaction of photosystem II exposes its key reaction center core protein subunits to irreversible oxidative photodamage. A rapid repair cycle replaces the photodamaged core subunits in plants, but how the large antenna-core supercomplex structures of plant photosystem II disassemble for repair is not currently understood. Here, we report the specific involvement of phosphorylation in removal of the peripheral antenna from the core and monomerization of the dimeric cores. However, monomeric cores disassemble further into smaller subcomplexes, even in the absence of phosphorylation, suggesting that there are other unknown mechanisms of disassembly. In this regard, we show that oxidative modifications of amino acids in core protein subunits of photosystem II are active mediators of monomeric core disassembly. Oxidative modifications thus likely disassemble only the damaged monomeric cores, ensuring an economical photosystem disassembly process. Taken together, our results suggest that phosphorylation and oxidative modification play distinct roles in photosystem II disassembly and repair.</p>","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101202"},"PeriodicalIF":9.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solanaceae pan-genomes reveal extensive fractionation and functional innovation of duplicated genes.","authors":"Lingkui Zhang, Yuanhang Liu, Yile Huang, Yiyue Zhang, Yu Fu, Ya Xiao, Shumin Chen, Kang Zhang, Feng Cheng","doi":"10.1016/j.xplc.2024.101231","DOIUrl":"10.1016/j.xplc.2024.101231","url":null,"abstract":"<p><p>The Solanaceae family contains many agriculturally important crops, including tomato, potato, pepper, and tobacco, as well as other species with potential for agricultural development, such as the orphan crops groundcherry, wolfberry, and pepino. Research progress varies greatly among these species, with model crops like tomato being far ahead. This disparity limits the broader agricultural application of other Solanaceae species. In this study, we constructed an interspecies pan-genome for the Solanaceae family and identified various gene retention patterns. Our findings reveal that the activity of specific transposable elements is closely associated with gene fractionation and transposition. The pan-genome was further resolved at the level of T subgenomes, which were generated by Solanaceae-specific paleo-hexaploidization (T event). We demonstrate substantial gene fractionation (loss) and divergence events following ancient duplications. For example, all class A and E flower model genes in Solanaceae originated from two tandemly duplicated genes, which expanded through the γ and T events before fractionating into 10 genes in tomato, each acquiring distinct functions critical for fruit development. Based on these results, we developed the Solanaceae Pan-Genome Database (SolPGD, http://www.bioinformaticslab.cn/SolPGD), which integrates datasets from both inter- and intra-species pan-genomes of Solanaceae. These findings and resources will facilitate future studies of solanaceous species, including orphan crops.</p>","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101231"},"PeriodicalIF":9.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956106/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PMGA: A plant mitochondrial genome annotator.","authors":"Jingling Li, Yang Ni, Qianqi Lu, Haimei Chen, Chang Liu","doi":"10.1016/j.xplc.2024.101191","DOIUrl":"10.1016/j.xplc.2024.101191","url":null,"abstract":"","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101191"},"PeriodicalIF":9.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering CO₂-fixing modules in Escherichia coli via efficient assembly of cyanobacterial Rubisco and carboxysomes.","authors":"Yaqi Sun, Taiyu Chen, Xingwu Ge, Tao Ni, Gregory F Dykes, Peijun Zhang, Fang Huang, Lu-Ning Liu","doi":"10.1016/j.xplc.2024.101217","DOIUrl":"10.1016/j.xplc.2024.101217","url":null,"abstract":"<p><p>Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) is the central enzyme for conversion of atmospheric CO₂ into organic molecules, playing a crucial role in the global carbon cycle. In cyanobacteria and some chemoautotrophs, Rubisco complexes, together with carbonic anhydrase, are enclosed within specific proteinaceous microcompartments known as carboxysomes. The polyhedral carboxysome shell ensures the dense packaging of Rubisco and creates a high-CO₂ internal environment to facilitate CO₂ fixation. Rubisco and carboxysomes have been popular targets for bioengineering, with the intent of enhancing plant photosynthesis, crop yields, and biofuel production. However, efficient generation of Form 1B Rubisco and cyanobacterial β-carboxysomes in heterologous systems remains a challenge. Here, we developed genetic systems to efficiently engineer functional cyanobacterial Form 1B Rubisco in Escherichia coli by incorporating Rubisco assembly factor Raf1 and modulating the RbcL/S stoichiometry. We then reconstituted catalytically active β-carboxysomes in E. coli with cognate Form 1B Rubisco by fine-tuning the expression levels of individual β-carboxysome components. In addition, we investigated the mechanism of Rubisco encapsulation into carboxysomes by constructing hybrid carboxysomes; this was achieved by creating a chimeric encapsulation peptide incorporating small sub-unit-like domains, which enabled the encapsulation of Form 1B Rubisco into α-carboxysome shells. Our study provides insights into the assembly mechanisms of plant-like Form 1B Rubisco and the principles of its encapsulation in both β-carboxysomes and hybrid carboxysomes, highlighting the inherent modularity of carboxysome structures. These findings lay the framework for rational design and repurposing of CO₂-fixing modules in bioengineering applications, e.g., crop engineering, biocatalyst production, and molecule delivery.</p>","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101217"},"PeriodicalIF":9.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of salt-responsive genetic variants using cross-condition multi-omics association analysis in maize.","authors":"Songyu Liu, Jing Yang, Can Yin, Shiya Mao, Qian Cheng, Jun Yan, Caifu Jiang, Xiangfeng Wang, Xiaoyan Liang, Haiming Zhao","doi":"10.1016/j.xplc.2024.101219","DOIUrl":"10.1016/j.xplc.2024.101219","url":null,"abstract":"","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101219"},"PeriodicalIF":9.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142808371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic marker-assisted genomic prediction improves hybrid breeding.","authors":"Yang Xu, Wenyan Yang, Jie Qiu, Kai Zhou, Guangning Yu, Yuxiang Zhang, Xin Wang, Yuxin Jiao, Xinyi Wang, Shujun Hu, Xuecai Zhang, Pengcheng Li, Yue Lu, Rujia Chen, Tianyun Tao, Zefeng Yang, Yunbi Xu, Chenwu Xu","doi":"10.1016/j.xplc.2024.101199","DOIUrl":"10.1016/j.xplc.2024.101199","url":null,"abstract":"<p><p>Hybrid breeding is widely acknowledged as the most effective method for increasing crop yield, particularly in maize and rice. However, a major challenge in hybrid breeding is the selection of desirable combinations from the vast pool of potential crosses. Genomic selection (GS) has emerged as a powerful tool to tackle this challenge, but its success in practical breeding depends on prediction accuracy. Several strategies have been explored to enhance prediction accuracy for complex traits, such as the incorporation of functional markers and multi-omics data. Metabolome-wide association studies (MWAS) help to identify metabolites that are closely linked to phenotypes, known as metabolic markers. However, the use of preselected metabolic markers from parental lines to predict hybrid performance has not yet been explored. In this study, we developed a novel approach called metabolic marker-assisted genomic prediction (MM_GP), which incorporates significant metabolites identified from MWAS into GS models to improve the accuracy of genomic hybrid prediction. In maize and rice hybrid populations, MM_GP outperformed genomic prediction (GP) for all traits, regardless of the method used (genomic best linear unbiased prediction or eXtreme gradient boosting). On average, MM_GP demonstrated 4.6% and 13.6% higher predictive abilities than GP for maize and rice, respectively. MM_GP could also match or even surpass the predictive ability of M_GP (integrated genomic-metabolomic prediction) for most traits. In maize, the integration of only six metabolic markers significantly associated with multiple traits resulted in 5.0% and 3.1% higher average predictive ability compared with GP and M_GP, respectively. With advances in high-throughput metabolomics technologies and prediction models, this approach holds great promise for revolutionizing genomic hybrid breeding by enhancing its accuracy and efficiency.</p>","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101199"},"PeriodicalIF":9.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142755900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geminivirus βV1 protein activates bZIP17/28-mediated UPR signaling to facilitate viral pathogenicity but its activity is attenuated by autophagic degradation in plants.","authors":"Tao Hu, Chenyang Li, Hui Liu, Chenlu Su, Yaqin Wang, Fangfang Li, Xueping Zhou","doi":"10.1016/j.xplc.2024.101198","DOIUrl":"10.1016/j.xplc.2024.101198","url":null,"abstract":"<p><p>The unfolded protein response (UPR) is a vital cellular pathway that maintains endoplasmic reticulum (ER) homeostasis under conditions of ER stress and is associated with the degradation of misfolded proteins. However, the role of ER-associated degradation in plant-microbe interactions has yet to be explored. In this study, we identified a novel viral protein, βV1, encoded by the tomato yellow leaf curl betasatellite (TYLCCNB), which is localized to the ER and triggers ER aggregation. Transient expression of βV1 in Nicotiana benthamiana induces robust ER stress and activates the bZIP17/28 branch of the UPR signaling pathway. The induction of bZIP17/28 by βV1 is crucial for successful virus infection. Furthermore, we demonstrated that βV1 is unstable in N. benthamiana mesophyll cells, as it is targeted for autophagic degradation. The autophagy-related protein ATG18a, a key component of autophagosomes, participates in the degradation of βV1, thereby exerting an anti-viral role. Taken together, our results reveal a novel function of the βV1 protein and provide the first evidence for involvement of bZIP17/28 and ATG18a in ER-associated autophagic degradation during geminivirus infection. These findings significantly expand our understanding of the arms-race dynamics between plants and viruses.</p>","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101198"},"PeriodicalIF":9.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142741218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenomics-assisted genetic dissection and molecular design of drought resistance in rice.","authors":"Qiaojun Lou, Yunyu Chen, Xin Wang, Yulu Zhang, Tingting Gao, Jiawei Shi, Ming Yan, Fangjun Feng, Kai Xu, Feng Lin, Shangyuan Xie, Xiaoyan Xi, Weikun Li, Yuanyuan Nie, Huan Gao, Hui Xia, Lei Wang, Tiemei Li, Shoujun Chen, Ying Zhu, Jianwei Zhang, Hanwei Mei, Liang Chen, Wanneng Yang, Lijun Luo","doi":"10.1016/j.xplc.2024.101218","DOIUrl":"10.1016/j.xplc.2024.101218","url":null,"abstract":"<p><p>Dissecting the mechanism of drought resistance (DR) and designing drought-resistant rice varieties are promising strategies to address the challenge of climate change. Here, we selected a typical drought-avoidant (DA) variety, IRAT109, and a drought-tolerant (DT) variety, Hanhui15, as parents to develop a stable recombinant inbred line (RIL) population (F₈, 1262 lines). The de novo assembled genomes of both parents were released. By resequencing of the RIL population, a set of 1 189 216 reliable SNPs were obtained and used to construct a dense genetic map. Using above- and belowground phenomic platforms and multimodal cameras, we captured 139 040 image-based traits (i-traits) of whole-plant phenotypes in response to drought stress throughout the entire rice growth period and identified 32 586 drought-responsive quantitative trait loci (QTLs), including 2097 unique QTLs. QTLs associated with panicle i-traits occurred more than 600 times on the middle of chromosome 8, and QTLs associated with leaf i-traits occurred more than 800 times on the 5' end of chromosome 3, indicating the potential effects of these QTLs on plant phenotypes. We selected three candidate genes (OsMADS50, OsGhd8, OsSAUR11) related to leaf, panicle, and root traits, respectively, and verified their functions in DR. OsMADS50 was found to negatively regulate DR by modulating leaf dehydration, grain size, and downward root growth. A total of 18 and 21 composite QTLs significantly related to grain weight and plant biomass were also screened from 597 lines in the RIL population under drought conditions in field experiments, and the composite QTL regions showed substantial overlap (76.9%) with known DR gene regions. Based on three candidate DR genes, we proposed a haplotype design suitable for different environments and breeding objectives. This study provides a valuable reference for multimodal and time-series phenomic analyses, deciphers the genetic mechanisms of DA and DT rice varieties, and offers a molecular navigation map for breeding of DR varieties.</p>","PeriodicalId":52373,"journal":{"name":"Plant Communications","volume":" ","pages":"101218"},"PeriodicalIF":9.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}