A bHLH7-PDF2 module mediates transcriptional regulation to repress seed oil accumulation in Arabidopsis thaliana.

IF 11.6 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Communications Pub Date : 2025-07-18 DOI:10.1016/j.xplc.2025.101459

Zijin Liu, Yu Zhao, Lianying Mao, Dong Li, Bo Song, Minshan Jin, Jun Ma, Jianjun Wang, Shuangcheng He, Shixiang Wang, Huan Hu, Yuan Guo, Saiqi Yang, Mingxun Chen

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引用次数: 0

Abstract

Seed oil accumulation requires precise coordination of multiple factors, and transcription factors (TFs) play crucial roles in governing this process. However, the knowledge of how TFs and the crosstalk between different TF families coordinate the transcriptional network associated with seed oil accumulation is still lacking. Here, we report that both basic helix-loop-helix7 (bHLH7) and PROTODERMAL FACTOR2 (PDF2) TFs, highly expressed in the developing embryos, negatively control seed oil accumulation. bHLH7 and PDF2 physically interact and cooperatively inhibit the transcription of MYB96, PYRUVATE DEHYDROGENASE E1 BETA1 (PDH-E1β1), PDH-E1β2, and BIOTIN CARBOXYL CARRIER PROTEIN1 (BCCP1) by directly binding to their promoters. Further genetic findings demonstrated that bHLH7 and PDF2 act in an additive manner to repress seed oil accumulation, and this effect is partially dependent on PDH-E1β1 that significantly promotes seed oil deposition. Collectively, we provide insights into the mechanisms by which transcriptional regulation mediated by the bHLH7-PDF2 module controls oil accumulation in A. thaliana seeds.

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bHLH7-PDF2模块介导转录调控抑制拟南芥种子油积累。

种子油脂的积累需要多种因素的精确协调，而转录因子在这一过程中起着至关重要的作用。然而，关于TF和不同TF家族之间的串扰如何协调与种子油积累相关的转录网络的知识仍然缺乏。在这里，我们报道了在发育中的胚胎中高度表达的基本螺旋环螺旋7 （bHLH7）和原胚层因子2 (PDF2) TFs对种子油积累具有负向控制作用。bHLH7和PDF2物理相互作用并协同抑制MYB96、丙酮酸脱氢酶E1 BETA1 （PDH-E1β1）、PDH-E1β2和生物素羧基载体蛋白1 （BCCP1）的转录，直接结合到它们的启动子上。进一步的遗传学研究表明，bHLH7和PDF2共同抑制种子油脂积累，这种作用部分依赖于显著促进种子油脂沉积的PDH-E1β1。总之，我们提供了bHLH7-PDF2模块介导的转录调控控制拟南芥种子油脂积累的机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Communications Agricultural and Biological Sciences-Plant Science

CiteScore

15.70

自引率

5.70%

发文量

105

审稿时长

6 weeks

期刊介绍： Plant Communications is an open access publishing platform that supports the global plant science community. It publishes original research, review articles, technical advances, and research resources in various areas of plant sciences. The scope of topics includes evolution, ecology, physiology, biochemistry, development, reproduction, metabolism, molecular and cellular biology, genetics, genomics, environmental interactions, biotechnology, breeding of higher and lower plants, and their interactions with other organisms. The goal of Plant Communications is to provide a high-quality platform for the dissemination of plant science research.