{"title":"The Pichia pastoris enzyme production platform: From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass","authors":"Korbinian Sinzinger , Ulrike Obst , Samed Güner , Manuel Döring , Magdalena Haslbeck , Doris Schieder , Volker Sieber","doi":"10.1016/j.jobab.2023.12.005","DOIUrl":"10.1016/j.jobab.2023.12.005","url":null,"abstract":"<div><p>The demand for industrial enzymes is continually rising, fueled by the growing need to shift towards more sustainable industrial processes. However, making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a challenge. Moreover, the production of the enzymes themselves comes with unavoidable impacts, e.g., the need to utilize secondary feedstocks. Here, we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting <em>Pichia pastoris</em> fermentation. We demonstrate these capabilities by producing a phytase secreted by <em>P. pastoris</em> that is grown on cyanobacterium hydrolysate and buffered glycerol-complex (BMGY) medium, with genetic expression conditions identified by high-throughput screening of a randomized secretion library. When our best-performing strain is grown in a fed-batch fermentation on BMGY, we reach over 7 000 U/mL in three days.</p></div>","PeriodicalId":52344,"journal":{"name":"Journal of Bioresources and Bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2369969823000798/pdfft?md5=4d815df1c0f82f9bd1238b3a592fec7a&pid=1-s2.0-S2369969823000798-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139189624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bianjing Sun , Ping Wang , Jingang Zhang , Jianbin Lin , Lingling Sun , Xiaokun Wang , Chuntao Chen , Dongping Sun
{"title":"In situ biosynthesis of bacterial cellulose hydrogel spheroids with tunable dimensions","authors":"Bianjing Sun , Ping Wang , Jingang Zhang , Jianbin Lin , Lingling Sun , Xiaokun Wang , Chuntao Chen , Dongping Sun","doi":"10.1016/j.jobab.2023.12.003","DOIUrl":"10.1016/j.jobab.2023.12.003","url":null,"abstract":"<div><p>Bacterial cellulose (BC) hydrogel spheroid plays a significant role in diverse fields due to its spatial 3D structure and properties. In the present work, a series of BC spheroids with controllable size and shape was obtained via an in situ biosynthesis. Crucial factors for fabricating BC spheroid including inoculum concentration of 1.35 × 10<sup>3</sup> CFU/mL, shaking speeds at 100 r/min, and 48–96 h incubation time during the biosynthetic process, were comprehensively established. An operable mechanism model for tuning the size of BC spheroids from 0.4 to 5.0 mm was proposed with a fresh feeding medium strategy of dynamic culture. The resulting BC spheroids exhibit an interactive 3D network of nanofibers, a crystallinity index of 72.3 %, a specific surface area of 91.2 m<sup>2</sup>/g, and good cytocompatibility. This study reinforces the understanding of BC spheroid formation and explores new horizons for the design of BC spheroids-derived functional matrix materials for medical care.</p></div>","PeriodicalId":52344,"journal":{"name":"Journal of Bioresources and Bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2369969823000774/pdfft?md5=ce966d5804f460d2a2cb954cc00d9a43&pid=1-s2.0-S2369969823000774-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139192245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Long Cheng, Shanyong Wang, Hailong Lu, Jun Ye, Junming Xu, Kui Wang, Jianchun Jiang
{"title":"Selective Activation of C–C Bonds in Lignin Model Compounds and Lignin for Production of Value-Added Chemicals","authors":"Long Cheng, Shanyong Wang, Hailong Lu, Jun Ye, Junming Xu, Kui Wang, Jianchun Jiang","doi":"10.1016/j.jobab.2024.02.001","DOIUrl":"https://doi.org/10.1016/j.jobab.2024.02.001","url":null,"abstract":"","PeriodicalId":52344,"journal":{"name":"Journal of Bioresources and Bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139823850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amal Mlhem , Thomas Teklebrhan , Evenezer Bokuretsion , Basim Abu-Jdayil
{"title":"Development of sustainable thermal insulation based on bio-polyester filled with date pits","authors":"Amal Mlhem , Thomas Teklebrhan , Evenezer Bokuretsion , Basim Abu-Jdayil","doi":"10.1016/j.jobab.2023.12.004","DOIUrl":"10.1016/j.jobab.2023.12.004","url":null,"abstract":"<div><p>Date palm pit (DPP)-filled poly (-hydroxybutyrate) (PHB) composites were prepared, evaluated, and characterized to determine their thermal insulation ability. Thermal conductivity values ranged between 0.086 and 0.100 W/(m·K). At a maximum filler concentration (50% (<em>w</em>)), the specific heat capacity and thermal diffusivity were 1 183 J/(kg·K) and 0.068 9 mm<sup>2</sup>/s, respectively. The DPP increased the thermal stability, and the highest compressive strength obtained was 80 MPa at 30% filler content. The PHB-DPP composites exhibited promising water absorption (less than 6%) and tensile strength (6–14 MPa). Date-pit-based PHB composites could be used in sustainable building engineering and cleaner production.</p></div>","PeriodicalId":52344,"journal":{"name":"Journal of Bioresources and Bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2369969823000786/pdfft?md5=8182a43f6405efc32ae5f51961c677f4&pid=1-s2.0-S2369969823000786-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139191438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sreesha Malayil , Luke Loughran , Frederik Mendoza Ulken , Jagannadh Satyavolu
{"title":"Exploring hemp seed hull biomass for an integrated C-5 biorefinery: Xylose and activated carbon","authors":"Sreesha Malayil , Luke Loughran , Frederik Mendoza Ulken , Jagannadh Satyavolu","doi":"10.1016/j.jobab.2024.01.002","DOIUrl":"10.1016/j.jobab.2024.01.002","url":null,"abstract":"<div><p>Large quantities of hemp hulls can be completely utilized for creation of value-added products (cost effective biofuels and biochemicals) through a biorefinery approach. A sustainable approach in making xylose, a low calorie sweetener and high surface area activated carbons (AC) for super capacitors, attracts interest. The AC when leveraged as a co-product from biorefinery process makes it more cost effective and, in this paper, we discuss the production of xylose and AC from hemp seed hull with methane sulphonic acid (MSA) hydrolysis. Xylose recovery with MSA hydrolysis was 25.15 g/L when compared to the traditional sulphuric acid (SA) hydrolysis of 19.96 g/L at the same acid loading of 1.8 %. The scanning electron microscope (SEM) images and Fourier transform infrared (FT-IR) spectra indicate partial delignification along with hemicellulose hydrolysis responsible for high xylose recovery. Post hydrolysis fibers were KOH activated and carbonized to make AC. The MSA hydrolyzed and KOH activated fiber produced pure, fluffier and finer particle AC with a drastic increase in surface area 1 452 m<sup>2</sup>/g when compared to SA hydrolyzed of 977 m<sup>2</sup>/g. These results indicate the potential of MSA in dilute acid hydrolysis of biomass for xylose recovery and production of high surface area activated carbon. From a production standpoint this can lead to increased use of sustainable low-cost agricultural biomass for making high surface area AC as components in supercapacitors.</p></div>","PeriodicalId":52344,"journal":{"name":"Journal of Bioresources and Bioproducts","volume":null,"pages":null},"PeriodicalIF":20.2,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2369969824000136/pdfft?md5=bc7ae392afcd02a6d3c33bb77a391044&pid=1-s2.0-S2369969824000136-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139637026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaohan Wang , Jinwon Jang , Yanqun Su , Jingang Liu , Hongjie Zhang , Zhibin He , Yonghao Ni
{"title":"Starting materials, processes and characteristics of bio-based foams: A review","authors":"Xiaohan Wang , Jinwon Jang , Yanqun Su , Jingang Liu , Hongjie Zhang , Zhibin He , Yonghao Ni","doi":"10.1016/j.jobab.2024.01.004","DOIUrl":"10.1016/j.jobab.2024.01.004","url":null,"abstract":"<div><p>Biofoam products have attracted considerable attention lately because there is a growing demand for green/sustainable products. To this end, various biobased foams have either been developed or are currently in development, e.g., bio-based polyurethanes (PUs), polylactic acid (PLA), starch, and polyhydroxyalkanotates (PHAs). Indeed, significant progress has been made; however, challenges still persist, for example, biobased foam products have poor processability, inferior compatibility, thermal and strength properties. In this review, we focus on five biofoam products: namely bio-based PUs, PLA, starch, PHAs, and cellulose biofoam products, along with their properties and performance, as well as their manufacturing processes. Further efforts are still needed to unlock the full potential of these bio-based products and meet the goal of complementing and gradually replacing some of their fossil-based counterparts. Finally, the challenges, as well as arising opportunities of future research directions are discussed.</p></div>","PeriodicalId":52344,"journal":{"name":"Journal of Bioresources and Bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S236996982400015X/pdfft?md5=08efe64aeef254b8f5d88074b01b7689&pid=1-s2.0-S236996982400015X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139632268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yunyi Liang , Yonghong Luo , Yingji Wu , Xiaona Li , Quyet Van Le , Jianzhang Li , Changlei Xia
{"title":"Nucleophilic amino acids as a renewable alternative to petrochemically-derived amines in glycerol epoxy resins","authors":"Yunyi Liang , Yonghong Luo , Yingji Wu , Xiaona Li , Quyet Van Le , Jianzhang Li , Changlei Xia","doi":"10.1016/j.jobab.2024.01.003","DOIUrl":"10.1016/j.jobab.2024.01.003","url":null,"abstract":"<div><p>The standard epoxy resin curing agents revealed are from unsustainable petroleum-based sources, which produce poisonous exhaust when cured. Amino acids, a bio-based epoxy curing agent with amino and carboxyl groups, are another potential curing agent. Water-soluble epoxy resins cured with lysine (Lys), glutamic acid (Glu), leucine (Leu), and serine (Ser) as amino acids were investigated. The results showed that the water-soluble epoxy resin (glycerol epoxy resins, GER) was cured with Lys and Glu after reacting. Fourier transform infrared (FT-IR) spectroscopic analysis of the GER-Lys showed that the amino and carboxyl groups of Lys primarily reacted with the epoxy groups of GER. The elongation at break of Lys-cured GER (GER-Lys) cured at 70 ℃ with a molar ratio of 1꞉0.75 was 75.32%. The fact that elongations at break of GER-Lys (79.43%) were higher than those of GER-Glu (17.33%), respectively supports the decrease of crosslinking density by the amino acid-cured GER reaction. The potential of Lys and Glu alternatives for petrochemical amines is demonstrated and provides promising opportunities for industrial application.</p></div>","PeriodicalId":52344,"journal":{"name":"Journal of Bioresources and Bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2369969824000148/pdfft?md5=27db72844385b0d2bb4b195e20552d24&pid=1-s2.0-S2369969824000148-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139639245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ryen M. Frazier , Keren A. Vivas , Ivana Azuaje , Ramon Vera , Alonzo Pifano , Naycari Forfora , Hasan Jameel , Ericka Ford , Joel J. Pawlak , Richard Venditti , Ronalds Gonzalez
{"title":"Beyond cotton and polyester: An evaluation of emerging feedstocks and conversion methods for the future of fashion industry","authors":"Ryen M. Frazier , Keren A. Vivas , Ivana Azuaje , Ramon Vera , Alonzo Pifano , Naycari Forfora , Hasan Jameel , Ericka Ford , Joel J. Pawlak , Richard Venditti , Ronalds Gonzalez","doi":"10.1016/j.jobab.2024.01.001","DOIUrl":"10.1016/j.jobab.2024.01.001","url":null,"abstract":"<div><p>As the global population grows, the demand for textiles is increasing rapidly. However, this puts immense pressure on manufacturers to produce more fiber. While synthetic fibers can be produced cheaply, they have a negative impact on the environment. On the other hand, fibers from wool, sisal, fique, wood pulp (viscose), and man-made cellulose fibers (MMCFs) from cotton cannot alone meet the growing fiber demand without major stresses on land, water, and existing markets using these materials. With a greater emphasis on transparency and circular economy practices, there is a need to consider natural non-wood alternative sources for MMCFs to supplement other fiber types. However, introducing new feedstocks with different compositions may require different biomass conversion methods. Therefore, based on existing work, this review addresses the technical feasibility of various alternative feedstocks for conversion to textile-grade fibers. First, alternative feedstocks are introduced, and then conventional (dissolving pulp) and emerging (fibrillated cellulose and recycled material) conversion technologies are evaluated to help select the most suitable and promising processes for these emerging alternative sources of cellulose. It is important to note that for alternative feedstocks to be adopted on a meaningful scale, high biomass availability and proximity of conversion facilities are critical factors. In North America, soybean, wheat, rice, sorghum, and sugarcane residues are widely available and most suitable for conventional conversion through various dissolving pulp production methods (pre-hydrolysis kraft, acid sulfite, soda, SO<sub>2</sub>-ethanol-water, and potassium hydroxide) or by emerging cellulose fibrillation methods. While dissolving pulp conversion is well-established, fibrillated cellulose methods could be beneficial from cost, efficiency, and environmental perspectives. Thus, the authors strongly encourage more work in this growing research area. However, conducting thorough cost and sustainability assessments is important to determine the best feedstock and technology combinations.</p></div>","PeriodicalId":52344,"journal":{"name":"Journal of Bioresources and Bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S236996982400001X/pdfft?md5=e0e2def54c2eca7e64bb6eb9918633e5&pid=1-s2.0-S236996982400001X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139394235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yufeng Yuan , Xinyu Guo , Bo Jiang , Wenjuan Wu , Tingwei Zhang , Michael Sweeney , Mehraj Ahmad , Yongcan Jin
{"title":"Effect of various aromatic compounds with different functional groups on enzymatic hydrolysis of microcrystalline cellulose and alkaline pretreated wheat straw","authors":"Yufeng Yuan , Xinyu Guo , Bo Jiang , Wenjuan Wu , Tingwei Zhang , Michael Sweeney , Mehraj Ahmad , Yongcan Jin","doi":"10.1016/j.jobab.2023.12.006","DOIUrl":"10.1016/j.jobab.2023.12.006","url":null,"abstract":"<div><p>Low molecular aromatic compounds are detrimental to the enzymatic hydrolysis of lignocellulose. However, the specific role of their functional groups remains unclear. Here, a series of nine aromatic compounds as additives were tested to understand their effect on the hydrolysis yield of microcrystalline cellulose (MCC) and alkaline pretreated wheat straw. Based on the results, the inhibition of aldehyde groups on MCC was greater than that of carboxyl groups, whereas for the alkaline pretreated wheat straw case, the inhibitory effect of aldehyde groups was lower than that of carboxyl groups. Increased methoxyl groups of aromatic compounds reduced the inhibitory effect on enzymatic hydrolysis of both substrates. Stronger inhibition of aromatic compounds on MCC hydrolysis was detected in comparison with the alkaline pretreated wheat straw, indicating that the substrate lignin can offset the inhibition to a certain extent. Among all aromatic compounds, syringaldehyde with one aldehyde group and two methoxyl groups improved the glucan conversion of the alkaline pretreated wheat straw.</p></div>","PeriodicalId":52344,"journal":{"name":"Journal of Bioresources and Bioproducts","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2369969823000804/pdfft?md5=1b86379a64c7686f6087a41b7e612ece&pid=1-s2.0-S2369969823000804-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139191307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biorenewable materials for water remediation: The central role of cellulose in achieving sustainability","authors":"Kirti Mishra , Samarjeet Singh Siwal , Thandiwe Sithole , Nirankar Singh , Phil Hart , Vijay Kumar Thakur","doi":"10.1016/j.jobab.2023.12.002","DOIUrl":"10.1016/j.jobab.2023.12.002","url":null,"abstract":"<div><p>As the population increases and manufacturing grows, greenhouse gas and other harmful emissions increase. Contaminated with chemicals such as dyes, pesticides, pharmaceuticals, oil, heavy metals or radionuclides, wastewater purification has become an urgent issue. Various technologies exist that can remove these contaminants from wastewater sources, but they often demand high energy and/or high cost, and in some cases produce contaminant laden sludge that requires safe disposal. The need for methods which are less capital intensive, less operationally costly and more environmentally friendly is suggested. Cellulose-based materials have emerged as promising candidates for wastewater treatment due to their renewability, low cost, biodegradability, hydrophilicity, and antimicrobial property. In this review article, we focussed on developing sustainable and biodegradable cellulose-based materials for wastewater treatment. This article deals with cellulose-based materials’ scope and their conversion into valuable products like hydrogel, aerogel, cellulose composites, and nanocellulose. The cellulose-based materials have no harmful environmental impact and are plentiful. The modified cellulose-based materials applying as membrane, adsorbent, sorbent, and beads to purify the wastewater were discussed. Finally, the challenges and future prospects of cellulose-based materials for wastewater treatment were considered, emphasizing their potential to be sustainable and eco-friendly alternatives to traditional materials used in wastewater treatment.</p></div>","PeriodicalId":52344,"journal":{"name":"Journal of Bioresources and Bioproducts","volume":null,"pages":null},"PeriodicalIF":20.2,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2369969823000762/pdfft?md5=199c498d373a0953dd6b51ac665b5518&pid=1-s2.0-S2369969823000762-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138627303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}