Annals of agricultural and environmental medicine : AAEM最新文献

{"title":"Clinical significance of serum ATP8B1 in children with Mycoplasma pneumoniae infection complicated with myocardial injury.","authors":"Xiaoya Li, Yanru Mi, Qinmei Lu, Congzhe Li","doi":"10.26444/aaem/215741","DOIUrl":"https://doi.org/10.26444/aaem/215741","url":null,"abstract":"<p><strong>Introduction and objective: </strong><i>Mycoplasma pneumoniae</i> (MP) infection can lead to various extrapulmonary complications, including myocardial injury (MI). However, the expression levels of ATPase phospholipid transporting 8B1 (ATP8B1) in MP-infected individuals with MI and its potential therapeutic role remain elusive. The aim of the study is to evaluate ATP8B1 as a therapeutic target for MP-induced MI.</p><p><strong>Material and methods: </strong>The study quantified ATP8B1 expression in patient serum via RT-qPCR and analyzed its diagnostic value for MP and MP+MI using receiver operating characteristic (ROC) curves. Binary logistic regression assessed its association with MP+MI. Serum interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) levels were measured by ELISA and correlated with ATP8B1. In a dual-cell model (MP-infected BEAS-2B cells and AC16 cells exposed to their supernatant), ATP8B1's effects on inflammation, proliferation, reactive oxygen species (ROS), and cardiac injury markers were evaluated via RT-qPCR, CCK-8, and ELISA.</p><p><strong>Results: </strong>The study revealed that serum ATP8B1 levels were significantly reduced in the MP group and further decreased in the MP+MI group. ATP8B1 expression levels could distinguish MP patients from MP+MI patients. Serum IL-6 and TNF-α levels were significantly higher in the MP+MI group than in the MP group, and ATP8B1 was negatively correlated with IL-6 and TNF-α. <i>In vitro</i>, ATP8B1 was downregulated in both cell lines. Functionally, over-expression of ATP8B1 effectively attenuated inflammation, ROS production, deficits in cell proliferation, and myocardial injury.</p><p><strong>Conclusions: </strong>ATP8B1 was expressed lowly in the serum of MP+MI patients. Functional experiments demonstrated that ATP8B1 upregulation significantly attenuated inflammation, ROS generation, proliferation deficits, and myocardial injury.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"385-392"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EBV DNA load in the saliva of healthy people in south-eastern Poland.","authors":"Karol Paradowski, Magdalena Góralczyk, Małgorzata Goździewska, Sylwester Bogacki, Małgorzata Polz-Dacewicz","doi":"10.26444/aaem/221046","DOIUrl":"https://doi.org/10.26444/aaem/221046","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Epstein-Barr virus (EBV) is widespread in the world's population. Transmitted mainly through saliva, the EBV virus infects the epithelial cells of the oropharynx, where it can replicate, establishing latency in B lymphocytes. Many authors analyse EBV viremia in saliva as a possible biomarker of various diseases. The aim of the study is to analyse the frequency and level of EBV viremia in the saliva of a healthy population of children and adults. The level of anti-EBV antibodies and a possible correlation between EBV viral load in saliva and antibody titres in serum were also examined.</p><p><strong>Material and methods: </strong>The study involved 66 healthy people, including 22 children aged 5-10 and 44 adults. EBV DNA was isolated from the saliva of all patients, and the presence and level of EBV DNA was detected using the qPCR method. Anti-EBV antibodies in serum were detected by ELISA.</p><p><strong>Results: </strong>The higher percentage of positive results was detected in children aged 5-10 (81.8%) and a smaller percentage in adults aged 20-49 (75.0%), this difference was not statistically significant. EBV DNA was most often detected in adults aged 50-70 (85.7%). The mean level of EBVCA IgG antibodies was similar in both age groups. Similarly, no difference in EBNA-1 antibody titers was observed between younger and older groups of adult patients. In three healthy people, despite the presence of EBV DNA in saliva, no anti-EBVCA or EBNA-1 antibodies were detected.</p><p><strong>Conclusions: </strong>The prevalence of EBV DNA in the healthy population ranged from 75% - 85.7%. Additionally, no correlation was observed between the EBV load in saliva and the titer of tested antibodies in the serum of healthy people.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"313-317"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of serum miR-95 in children with <i>Mycoplasma pneumoniae</i> infection complicated with diarrhea.","authors":"Hongliang Zhang, Mengyang Cai, Qing Gong","doi":"10.26444/aaem/217203","DOIUrl":"https://doi.org/10.26444/aaem/217203","url":null,"abstract":"<p><strong>Introduction and objective: </strong><i>Mycoplasma pneumoniae</i> infection (MPI) can lead to extrapulmonary diseases, in which diarrhea occurs frequently. The role and impact of microRNA-95 (miR-95) in MPI and MPI with diarrhea (MPI+DIA) remain elusive. The aim of the study is to investigate miR-95 expression and its functional impact in paediatric MPI with diarrhea.</p><p><strong>Material and methods: </strong>The study enrolled 80 MPI patients (53 without diarrhea, 27 with diarrhea) and 80 matched healthy controls. Expression of miR-95 was quantified using reverse transcription quantitative polymerase chain reaction (RT -qPCR). Predictors of disease status were analyzed through binary logistic regression. An <i>in vitro</i> pneumonia model (MP-BEAS-2B) was generated by infecting BEAS-2B cells with <i>Mycoplasma pneumoniae</i> (MP). In parallel, an intestinal epithelial injury model was generated by treating Caco-2 cells with 4 μg/mL lipid-associated membrane proteins (LAMPs). Cell proliferation was measured with cell counting kit-8 (CCK-8), apoptosis by flow cytometry, and interleukin -8 (IL-8) and tumour necrosis factor-alpha (TNF-α) levels were determined via RT-qPCR.</p><p><strong>Results: </strong>The study revealed that miR-95 was significantly elevated in the MPI group, with a further increase observed in MPI patients complicated with diarrhea. Additionally, miR-95 served as a discriminator among healthy controls, MPI patients, and MPI+DIA patients. In MP-BEAS-2B and LAMPs-Caco-2 cells, miR-95 was highly expressed. However, downregulating miR-95 significantly enhanced cell proliferation, inhibited apoptosis, and reduced the expression of IL-8 and TNF-α.</p><p><strong>Conclusions: </strong>MiR-95 expression was significantly elevated in MPI patients, and further increased in those with MPI complicated by diarrhea. Downregulating miR-95 significantly protected BEAS-2B and Caco-2 cells from MP damage and alleviated cellular inflammation.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"407-412"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The diagnostic value of miR-1-3p in atopic dermatitis and its correlation with inflammatory factors.","authors":"Lun Pan, Jianping He, Peng Han, Juan Wang","doi":"10.26444/aaem/221076","DOIUrl":"https://doi.org/10.26444/aaem/221076","url":null,"abstract":"<p><strong>Introduction and objective: </strong>MicroRNAs (miRNAs), such as miR-1-3p, play a crucial role in inflammatory processes, including atopic dermatitis (AD). The aim of the study is to investigate the role of miR-1-3p in AD, and its correlation with inflammation.</p><p><strong>Material and methods: </strong>miR-1-3p level was analyzed using qRT-PCR. Its diagnostic value was assessed via ROC curve analysis. Correlations between miR-1-3p and inflammatory factors were assessed by Spearman's rank correlation coefficient. Proliferation of HaCaT cells were detected by CCK-8. The influence of miR-1-3p for inflammatory factors were examined through enzyme-linked immunosorbent assay (ELISA). Target genes of miR-1-3p were predicted using bioinformatics databases.</p><p><strong>Results: </strong>miR-1-3p was upregulated in AD patients and had a high diagnostic value (area under ROC curve=0.927, sensitivity=0.830, specificity=0.934) for AD. miR-1-3p was negatively correlated with lymphocytes (r=-0.558), and positively correlated with CRP (r=0.570), IL-6 (r=0.511), and IL-22 (r=0.596) levels, respectively. A high miR-1-3p level in HaCaT cells suppressed proliferation and increased inflammatory cytokine levels. Bioinformatics analysis identified 61 ovrerlapping target genes of miR-1-3p, enriched in pathways associated with inflammation and immune response.</p><p><strong>Conclusions: </strong>miR-1-3p is elevated in AD and associated with inflammation, suggesting a role in AD pathogenesis. Its effects on HaCaT cells and correlation with inflammatory markers indicate a mechanistic involvement in AD.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"367-372"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of lncRNA SNHG7 in osteogenic differentiation of bone marrow mesenchymal stem cells.","authors":"Wei Wang, Jing Liu, Yanning Luo","doi":"10.26444/aaem/218949","DOIUrl":"https://doi.org/10.26444/aaem/218949","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Bone marrow mesenchymal stem cells (BMSCs), as an ideal cell source for bone regeneration, are crucial in the osteogenic differentiation. The aim of the article is to explore the effects of lncRNA SNHG7 on osteogenic differentiation and its regulation of miR-214-5p.</p><p><strong>Material and methods: </strong>Alkaline phosphatase (ALP), osterix, runt-related transcription factor 2 (RUNX2), and osteocalcin (OCN) were measured to assay the regulating function of lncRNA SNHG7 in osteogenic differentiation in human BMSCs (HBMCs). Late osteogenic markers were assayed by a quantitative real-time polymerase chain reaction (qRT-PCR) and an ALP Activity Assay Kit. The dual-luciferase reporter assay was used to identify the binding relationship between lncRNA SNHG7 and miR-214-5p, or miR-214-5p and BMP2.</p><p><strong>Results: </strong>LncRNA SNHG7 was upregulated in osteogenic differentiation of HBMSCs and was time-dependent. Overexpression of lncRNA SNHG7 increased the level of ALP, RUNX2, osterix, and OCN. LncRNA SNHG7 targeted and negatively regulated miR-214-5p. When lncRNA SNHG7 was inhibited, miR-214-5p was upregulated. The downregulation of osteogenic markers (OCN, osterix, and RUNX2) caused by suppressing lncRNA SNHG7 was reversed by transfecting the miR-214-5p inhibitor. Additionally, the BMP2 was reduced by overexpressing lncRNA SNHG7, and the regulating effects of lncRNA SNHG7 on BMP2 were reversed by overexpressing miR-214-5p.</p><p><strong>Conclusions: </strong>LncRNA SNHG7 could increase the level of BMP2 via downregulating miR-214-5p to promote osteogenic differentiation in HBMSCs. LncRNA SNHG7 may be a promoting factor of the osteogenic differentiation of HBMSCs after being upregulated.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"359-366"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of Tanshinone IIA in affecting miR-499-5p/PDCD4 axis in diabetic foot-related endothelial cells.","authors":"Yu Ming, Wei Zhao, Bin Cao, Haiyan Tang, Jiuying Ji, Yongquan Cao, Shanshan Lu","doi":"10.26444/aaem/217674","DOIUrl":"10.26444/aaem/217674","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Diabetic foot wound healing is a significant challenge for diabetic patients. Tanshinone IIA (Tan IIA) is widely used in treating diabetes and related complications, but its role in diabetic foot wound healing remains unclear. The aim of the study is to investigate the mechanism of Tan IIA in diabetic foot wound healing.</p><p><strong>Material and methods: </strong>Human umbilical vein endothelial cells (HUVECs) cultured in high-glucose medium were used to simulate diabetic pathological conditions. Cell viability and migration were detected by MTT and transwell assays while Annexin V-FITC/PI was used for apoptosis analysis. Measurement of nitric oxide (NO) and Endothelin-1 (ET-1) were performed by ELISA. The expression of vascular endothelial growth factor (VEGF), miR-499-5p and PDCD4 was calculated by RT-qPCR. Dual-luciferase reporter assay was used to validate the interaction of miR-499-5p and PDCD4.</p><p><strong>Results: </strong>Tan IIA protected high-glucose-treated HUVECs by enhancing cell viability and migration, reducing apoptosis and oxidative stress, and improving endothelial function. Similar effects were observed when miR-499-5p was upregulated. Knockdown of miR-499-5p abolished the protective effects of Tan IIA. PDCD4 was identified as a direct target of miR-499-5p.</p><p><strong>Conclusions: </strong>Tanshinone IIA protects high glucose-induced HUVECs by enhancing viability, migration, and endothelial function, while reducing apoptosis and oxidative stress, via the miR-499-5p/PDCD4 axis.These in vitro findings support its potential in diabetic foot wound healing, pending in vivo validation.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"325-332"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do <i>Aloe vera</i>, <i>Bryophyllum pinnatum,</i> <i>Callisia fragrans</i>, and <i>Pelargonium graveolens</i>, used in folk medicine to treat skin diseases, exhibit antioxidant activity?","authors":"Anna Horecka, Anna Olszewska, Sebastian Mertowski, Jerzy Kuliński, Anna Hordyjewska","doi":"10.26444/aaem/216269","DOIUrl":"https://doi.org/10.26444/aaem/216269","url":null,"abstract":"<p><strong>Introduction and objective: </strong><i>Aloe vera</i>, <i>Bryophyllum pinnatum</i>, <i>Callisia fragrans</i> and <i>Pelargonium graveolens</i> are medicinal plants traditionally used in folk medicine to treat a variety of diseases. The aim of the study is to evaluate the antioxidant properties of <i>Callisia fragrans</i>, and to the best of the authors' knowledge is the first such study on the topic.</p><p><strong>Material and methods: </strong>The focus of the study was on the antioxidant activities of plant ethanol extracts in an <i>in vitro</i> model of the mouse L929 fibroblastic cell line, in which oxidative stress was induced by hydrogen peroxide (H₂O₂). Extracts were obtained from biennial plants? Fibroblasts were first stimulated with 100 µM H₂O₂ for 3 h and subsequently incubated with various concentrations of plant extracts: 3.5 g/ml (extract 1); 1.75 g/ml (extract 2); and 0.875 g/ml (extract 3) for 24 h.</p><p><strong>Results: </strong>A significant decrease in ferric reducing antioxidant power (FRAP) and malondialdehyde (MDA) were observed in cell lysates treated with <i>Aloe vera</i>, <i>Bryophyllum pinnatum</i> and <i>Pelargonium graveolens</i> extracts. The concentration of carbonyl groups was statistically lower in cell lysates treated with <i>Aloe vera</i> and <i>Pelargonium graveolens</i> extracts. Significant increase in protein carbonyl groups concentration was observed in cell lysates treated with <i>Callisia fragrans</i>, but no significant changes were noticed in MDA and FRAP concentrations.</p><p><strong>Conclusions: </strong>The results obtained confirmed antioxidant activities of <i>Aloe vera</i>, <i>Bryophyllum pinnatum</i> and <i>Pelargonium graveolens</i>, which may contribute to their protective effects in cutaneous diseases. They also have the ability to protect fibroblasts in skin by a decrease in ROS production, and by the inhibition of lipid and protein oxidation. Therefore, they can be used in dermatology for the treatment of cutaneous diseases in the form of external skin care products. <i>Callisia fragrans</i>, however, showed the opposite trend, indicating a possible pro-oxidant behaviour requiring further investigation.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"347-352"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-483-5p promotes uterine leiomyoma progression by targeting TIMP2.","authors":"Yanhui Fang, Wenyu Pan, Junqiao Jiang, Jing Hu, Qiuxia Xu, Liyuan Huang, Yating Chen","doi":"10.26444/aaem/219468","DOIUrl":"https://doi.org/10.26444/aaem/219468","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Uterine leiomyoma (UL) is a prevalent tumour of the female reproductive system which is poorly understood in terms of pathogenesis, and treatment options are limited. MicroRNAs (miRNAs) have been identified as being associated with tumorigenesis. The aim of the study is to elucide the function of miR-483-5p in promoting UL progression by targeting TIMP metallopeptidase inhibitor 2 (TIMP2) and its underlying molecular mechanisms.</p><p><strong>Material and methods: </strong>The study included 340 patients with UL. The expression level of miR-483-5p was measured using quantitative real-time PCR (qPCR). Following knockdown of miR-483-5p in uterine leiomyoma cells (UtLMCs), cell proliferation, migration, and apoptosis were assessed using CCK-8, Transwell, and flow cytometry assays. Subsequently, bioinformatics analysis and luciferase reporter gene detection identified the target of miR-483-5p.</p><p><strong>Results: </strong>Compared to the adjacent myometrium, miR-483-5p was upregulated in UL tissues. Experiments revealed that miR-483-5p expression levels were increased in UtLMCs. It was determined that TIMP2 is the direct target of miR-483-5p. Furthermore, miR-483-5p significantly promoted the proliferation and migration of UtLMCs, and inhibited their apoptosis by targeting TIMP2.</p><p><strong>Conclusions: </strong>Collectively, these findings reveal the molecular mechanism by which miR-483-5p negatively regulates TIMP2 to promote UL development, providing new insights for the targeted therapy of UL.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"431-438"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xiangsha Weiling Pills alleviate intestinal barrier damage via the TGFB1/IL6 in diarrhea with syndrome of spleen deficiency with dampness encumbrance.","authors":"Zhanwei Liu, Guanglei Wang, Kexia Qin, Yihua Zhang, Pengcheng Geng, Huangliang Shen","doi":"10.26444/aaem/215740","DOIUrl":"https://doi.org/10.26444/aaem/215740","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Diarrhea with syndrome of spleen deficiency with dampness encumbrance (D-SSDDE) is a clinically debilitating condition, and the therapeutic mechanisms of Xiangsha Weiling Pills (XsWlP) against it remain unclear. The aim of the study is to explore the multidimensional regulatory mechanisms of XsWlP on D-SSDDE, and predict the key targets of its therapeutic effects using network pharmacology.</p><p><strong>Material and methods: </strong>In the D-SSDDE mouse model, physiological disorders were assessed by measuring loose stool rates and abdominal temperature, and using enzyme-linked immunosorbent assay (ELISA) to measure serum triglyceride (TG) levels. Gut barrier function was assessed by detecting goblet cell proportions using flow cytometry and quantifying tight junction protein (ZO-1, Claudin-1, MUC2) expression levels via reverse transcription quantitative polymerase chain reaction (RT-qPCR). The potential targets of XsWlP were predicted using network pharmacology. Additionally, we quantified interleukin 6 (IL6) and transforming growth factor beta 1 (TGFB1) expression levels in mouse intestinal tissues and serum via RT-qPCR and ELISA.</p><p><strong>Results: </strong>XsWlP substantially reverses D-SSDDE-induced metabolic disruptions, including reduced loose stool rate, restored abdominal temperature, and normalized serum TG levels, while repairing gut barrier integrity by adjusting goblet cell ratio and inducing tight junction proteins. Molecular docking validates TGFB1 and IL6 binding affinity. Moreover, XsWlP markedly suppresses the aberrant expression of D-SSDDE-induced pro-inflammatory factors, such as IL-6 and TGF-β1.</p><p><strong>Conclusions: </strong>The study demonstrates that XsWlP reduces intestinal barrier disruption in D-SSDDE via TGFB1/IL-6 signaling.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"339-346"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical significance of miR-218-5p and its potential mechanism in acute pulmonary embolism.","authors":"Rui Jin, Jinping Tong, Aiwen Zhang, Fang Liu","doi":"10.26444/aaem/217860","DOIUrl":"https://doi.org/10.26444/aaem/217860","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Acute pulmonary embolism (APE), a life-endangering cardiovascular acute disorder, brings about difficulties in early diagnosis. The aim of the study is to investigate the expression of miR-218-5p, its clinical significance, and mechanism in APE.</p><p><strong>Material and methods: </strong>A total of 102 APE patients and 98 healthy controls were recruited, with miR-218-5p levels assayed by qRT-PCR; its diagnostic value was assessed by ROC curves. Pearson correlation and multivariate logistic regression analyzed associations with clinical indicators and independent predictive value. Oxygen-glucose deprivation/reoxygenation (OGD/R)-induced human pulmonary artery endothelial cells (HPAECs) were transfected with miR-218-5p mimic to observe the effects on apoptosis, proliferation, inflammation, and oxidative stress. Bioinformatics and dual-luciferase assays validated miR-218-5p targeting CREB1.</p><p><strong>Results: </strong>Serum miR-218-5p was significantly downregulated in APE (P<0.0001) with high diagnostic efficacy (AUC=0.893). It positively correlated with D-dimer (r=0.759) and Wells scores (r=0.703; P<0.0001) and was an independent APE risk factor (OR=0.053; P<0.0001). In vitro, miR-218-5p overexpression reduced OGD/R-induced HPAEC apoptosis, promoted proliferation, inhibited inflammation (IL-6, IL-1β, TNF-α) and oxidative stress (MDA, ROS), and restored SOD activity (P<0.01). Mechanistically, miR-218-5p directly targeted CREB1 to suppress its expression.</p><p><strong>Conclusions: </strong>miR-218-5p is downregulated in APE, as a potential diagnostic biomarker. It targets CREB1 to modulate apoptosis, inflammation, and oxidative stress, thereby contributing to APE pathogenesis.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"293-299"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}