Annals of agricultural and environmental medicine : AAEM最新文献

Serum miR-155 as a biomarker for risk assessment and prediction of chronic complications in type 2 diabetes mellitus. 血清miR-155作为2型糖尿病慢性并发症风险评估和预测的生物标志物

IF 1.2

Annals of agricultural and environmental medicine : AAEM Pub Date : 2026-09-21 Epub Date: 2026-04-03 DOI: 10.26444/aaem/218267

Fang Zhou, Lanlan Chen, Meng Xie, Sen Meng, Jiaze Sun

{"title":"Serum miR-155 as a biomarker for risk assessment and prediction of chronic complications in type 2 diabetes mellitus.","authors":"Fang Zhou, Lanlan Chen, Meng Xie, Sen Meng, Jiaze Sun","doi":"10.26444/aaem/218267","DOIUrl":"https://doi.org/10.26444/aaem/218267","url":null,"abstract":"Introduction and objective: T2DM is a chronic disease with high prevalence. It can lead to a series of complications, causing severe damage to the body. The aim of the study is to examine the expression of miR-155 in patients with prediabetes and T2DM, and assessment of its diagnostic value.Material and methods: The biochemical indices of 96 prediabetic patients, 96 patients with T2DM, and 60 healthy subjects were measured using related instruments. The serum expression level of miR-155 in these three groups was detected through RT-qPCR. The early diagnostic value of miR-155 for T2DM was evaluated using a Receiver Operating Characteristic (ROC) curve. The predictive value of miR-155 for chronic complications in T2DM patients was assessed using a Kaplan-Meier curve. A logistic regression analysis was used to further assess the impact of miR-155 and other factors on T2DM complications.Results: The expression level of miR-155 was found to be down-regulated in the serum of patients with pre-diabetes and T2DM. MiR-155 demonstrated a significant ability to differentiate between prediabetic, T2DM, and healthy individuals. T2DM patients with low miR-155 expression were found to be more susceptible to complications. Therefore, miR-155 can be considered a risk factor for T2DM complications.Conclusions: The study highlighted the potential use of miR-155 in the early diagnosis and prognosis of T2DM. Additionally, miR-155 is somewhat associated with the onset and progression of T2DM complications.","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"413-417"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The miR-877-3p polymorphism rs1264440 is associated with susceptibility to breast cancer. miR-877-3p多态性rs1264440与乳腺癌易感性相关。

IF 1.2

Annals of agricultural and environmental medicine : AAEM Pub Date : 2026-09-21 Epub Date: 2026-05-08 DOI: 10.26444/aaem/220507

Wen Gong, Miao Yu, Jingjing Han, Xin Wang, Xiaoyun Mao

{"title":"The miR-877-3p polymorphism rs1264440 is associated with susceptibility to breast cancer.","authors":"Wen Gong, Miao Yu, Jingjing Han, Xin Wang, Xiaoyun Mao","doi":"10.26444/aaem/220507","DOIUrl":"https://doi.org/10.26444/aaem/220507","url":null,"abstract":"Introduction and objective: Breast cancer (BC) remains the most common malignant tumour among females. Dysregulated microRNAs (miRNAs) are key regulators in carcinogenesis, and single nucleotide polymorphisms (SNPs) represent their most prevalent genetic alterations. The aim of the study is to investigate the influence of rs1264440 on miR-877-3p expression and its subsequent effects on BC cell proliferation and migration.Material and methods: Both 160 female BC patients and 160 healthy control (HC) subjects in Maternity and Child Care Center of Qinhuangdao, China, were selected as case and control groups, respectively. Genotyping for the SNP rs1264440 was performed using a TaqMan probe-based RT-qPCR assay. The Cell Counting Kit-8 (CCK-8) assay was used to determine cell viability. Analysis of cell migration and invasion was conducted by transwell assays.Results: rs1264440 AA genotype (P = 0.006, OR = 0.308, 95% CI=0.129-0.739), A allele (P = 0.003, OR = 0.586, 95% CI = 0.413-0.832) significantly correlated with BC susceptibility. The rs1264440 AA genotype was associated with decreased miR-877-3p levels. High miR-877-3p level could distinguish BC patients from controls with an area under the curve (AUC) of 0.854 (sensitivity=73.13%, specificity=84.38%). Inhibition of miR-877-3p suppressed the proliferative, migratory, and invasive capacities of BC cells in vitro.Conclusions: The miR-877-3p rs1264440 is a risk predictor for BC, being implicated in miR-877-3p dysregulation and the promotion of cell viability and motility.","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"446-452"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of climatic factors and air quality on the temporal patterns of suicide attempts in Balıkesir, Turkey (2017-2024). 气候因素和空气质量对土耳其Balıkesir（2017-2024）自杀企图时间模式的影响

IF 1.2

Annals of agricultural and environmental medicine : AAEM Pub Date : 2026-09-21 Epub Date: 2026-06-30 DOI: 10.26444/aaem/224331

Duygu Kavuncuoglu, Meryem Betos Koçak, Sinan Yılmaz, Abdullah Osman Koçak

{"title":"The role of climatic factors and air quality on the temporal patterns of suicide attempts in Balıkesir, Turkey (2017-2024).","authors":"Duygu Kavuncuoglu, Meryem Betos Koçak, Sinan Yılmaz, Abdullah Osman Koçak","doi":"10.26444/aaem/224331","DOIUrl":"https://doi.org/10.26444/aaem/224331","url":null,"abstract":"Introduction and objective: The aim of this ecological study is to evaluate the potential relationships between the incidence of suicide attempts and climatic factors, as well as air quality parameters, in Balikesir, Turkey.Material and methods: Daily counts of cases of attempted suicide, climate, and air quality data were used. Prior to analysis, logarithmic transformation was applied to the data in order to stabilize variance. Data analysis was performed in EViews 10 using descriptive statistics (annual, monthly, weekly patterns) and ARMA error regression (ARIMAX). Stationarity was assessed with the ADF test, while the basic model structure was determined using ACF/PACF plots.Results: A total of 1,413 cases of attempted suicide, with a mean age of 26.9±14.2 years and 64.0% females, were included. Case distributions revealed a bimodal seasonal pattern (spring/early summer and winter) and a weekly rhythm with a marked decrease on Thursdays. Time series analyses indicated a strong association between the incidence of suicide attempts and its own short-term past values, with the ARMA(1,1) model providing the best fit. None of the lagged climatic, air quality, or seasonal dummy variables added to the model had a statistically significant effect on daily incidence (p>0.05).Conclusions: The findings of this study suggest that the predominant factor shaping the daily course of the incidence of suicide attempt in Balıkesir is the short-term autocorrelation structure of the series itself. The temporal dynamics and the potential effects of environmental factors may be more likely to operate through indirect mechanisms or cumulative influences, rather than immediate fluctuations.","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"333-338"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nurse competence development through professional entitlements in the Skill-Mix Model in rural areas - a review of national and international practices. 通过农村地区技能组合模式中的专业权利发展护士能力——对国家和国际实践的回顾。

IF 1.2

Annals of agricultural and environmental medicine : AAEM Pub Date : 2026-09-21 Epub Date: 2026-05-14 DOI: 10.26444/aaem/221073

Marika Guzek, Paweł Żuk, Monika Kaczoruk, Aleksandra Prusaczyk-Świerzewska, Jakub Owoc, Jerzy Kuliński, Magdalena Łoś

{"title":"Nurse competence development through professional entitlements in the Skill-Mix Model in rural areas - a review of national and international practices.","authors":"Marika Guzek, Paweł Żuk, Monika Kaczoruk, Aleksandra Prusaczyk-Świerzewska, Jakub Owoc, Jerzy Kuliński, Magdalena Łoś","doi":"10.26444/aaem/221073","DOIUrl":"10.26444/aaem/221073","url":null,"abstract":"Introduction and objective: In the light of the growing complexity of healthcare delivery, it is important to emphasise the need for efficient and safe nursing care underpinned by high professional standards. Collaboration is key to preventing problems. The aim of the study is to review how nurses could improve their roles, and examines what is being done nationally and internationally, as well as in rural areas where access to healthcare is often more challenging.Review methods: Evidence synthesis was based on narrative analysis and a state-of-the-art review, to summarise the field of nursing competences. As the article is narrative, a systematic review and study quality assessment were not used.Brief description of the state of knowledge: In recent decades, nursing has evolved from being auxiliary staff to becoming an autonomous profession, with nurses now being recognised as independent medical professionals. The Advanced Practice Nurse (APN) pathway is now a desirable career option in over 70 countries. In response to an increase in areas with limited access to healthcare and a shortage of nursing staff, a model of intersecting competencies is being implemented ('skill-mix'), offering nurses greater autonomy and an expanded skillset, including leadership.Summary: The effective utilization of the competencies of different healthcare professionals requires careful categorization of patients' health-related problems and alignment with the available medical staff. The key to optimising the potential of nursing staff lies in acknowledging their pivotal role within the healthcare system. By leveraging their expertise in investigating and assessing patient health, the quality of treatment and boosting of staff morale can be enhanced, and ensure patient satisfaction.","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"463-471"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of miR-802 in glaucoma and its diagnostic significance. miR-802在青光眼中的表达及其诊断意义

IF 1.2

Annals of agricultural and environmental medicine : AAEM Pub Date : 2026-09-21 Epub Date: 2026-05-15 DOI: 10.26444/aaem/219307

Tingting Zhang, Yufei Wang

{"title":"Expression of miR-802 in glaucoma and its diagnostic significance.","authors":"Tingting Zhang, Yufei Wang","doi":"10.26444/aaem/219307","DOIUrl":"https://doi.org/10.26444/aaem/219307","url":null,"abstract":"Introduction and objective: Glaucoma ranks as the second leading cause of blindness globally. The key factors leading to glaucoma are increased intraocular pressure and retinal ganglion cell apoptosis. Glaucoma is an irreversible disease that causes blindness in the eye, characterized by progressive optic nerve damage and visual field defects. The expression of miR-802 is abnormal in the plasma of glaucoma patients, but its function and mechanism remain unclear. The aim of the study is to investigate the expression of miR-802 in glaucoma and retinal ganglion cells, and the effect of miR-802 on 661W cells under oxidative stress.Material and methods: The study used RT-qPCR to detect the expression level of miR-802 in 661W cells and glaucoma patients. Receiver operating characteristic curve (ROC) was used to assess the diagnostic value of miR-802 in glaucoma. Cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay, while cell apoptosis was detected via flow cytometry.Results: In glaucoma patients, miR-802 expression was significantly downregulated and correlated with elevated IOP, reduced RNFL, and increased eGFR. MiR-802 demonstrated exceptional diagnostic value for the onset of glaucoma. In addition, decreased miR-802 expression was a risk factor associated with glaucoma. MiR-802 accelerated the proliferation of 661W cells and slowed down the apoptosis rate. Moreover, miR-802 mimic inhibited H2O2-induced 661W cell apoptosis.Conclusions: Upregulation of miR-802 alleviated oxidative stress-induced damage in 661W cells. miR-802 might be used as a marker for detecting glaucoma.","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"425-430"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The clinical role of miR-10b-5p in vascular calcification of diabetic nephropathy undergoing maintenance haemodialysis. miR-10b-5p在糖尿病肾病维持性血液透析血管钙化中的临床作用

IF 1.2

Annals of agricultural and environmental medicine : AAEM Pub Date : 2026-09-21 Epub Date: 2026-03-19 DOI: 10.26444/aaem/217998

Jing Ji, Yudong Fan, Di Wu

{"title":"The clinical role of miR-10b-5p in vascular calcification of diabetic nephropathy undergoing maintenance haemodialysis.","authors":"Jing Ji, Yudong Fan, Di Wu","doi":"10.26444/aaem/217998","DOIUrl":"https://doi.org/10.26444/aaem/217998","url":null,"abstract":"Introduction and objective: The aim of the study is to investigate the clinical role of miR-10b-5p in vascular calcification (VC) of diabetic nephropathy (DN) patients undergoing maintenance haemodialysis (MHD).Material and methods: In this study, 156 patients with DN undergoing MHD were enrolled into the study. Based on coronary angiography results, they were divided into the VC group (n = 93) and the non-VC group (n = 63). miR-10b-5p levels were obtained by RT-qPCR. The relationship between miR-10b-5p and PTH, CRP, and Ca-P product levels was analyzed by Pearson correlation analysis. Multivariate logistic regression was utilized to analyze the independent risk factors of VC in DN patients undergoing MHD. ROC curves were utilized to analyze the efficacy of serum miR-10b-5p combined with PTH, CRP, and Ca-P product levels in predicting VC in DN patients undergoing MHD.Results: miR-10b-5p was decreased in DN patients undergoing MHD with VC, while PTH, CRP, and Ca-P product were significantly elevated. In addition, in VC patients, miR-10b-5p was negatively correlated with PTH, CRP, and Ca-P product. Multivariate regression analysis revealed that miR-10b-5p, PTH, CRP, and Ca-P product are independent risk factors of VC occurrence in DN patients undergoing MHD. The ROC curves showed that serum miR-10b-5p combined with PTH, CRP, and Ca-P product levels had high diagnostic value for VC occurrence in DN patients undergoing MHD.Conclusions: A low level of miR-10b-5p may be a potential risk factor for VC occurrence in DN patients undergoing MHD. The combination of miR-10b-5p with PTH, CRP, and Ca-P product shows potential as clinical diagnostic biomarkers for the occurrence of VC in DN patients undergoing MHD.","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"300-304"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Public response to the proposed 'Health Education' subject in schools in Poland - attitudes, curriculum familiarity, and information sources. 公众对波兰学校拟议的“健康教育”科目的反应——态度、课程熟悉程度和信息来源。

IF 1.2

Annals of agricultural and environmental medicine : AAEM Pub Date : 2026-09-21 Epub Date: 2026-07-22 DOI: 10.26444/aaem/225377

Barbara Kołłątaj, Witold Piotr Kołłątaj, Justyna Wasiewicz, Agata Bryła Agata, Katarzyna Kłosowska, Wiktoria Kosek, Julia Justyna Soboń, Oktawia Maria Więckowska, Irena Dorota Karwat

{"title":"Public response to the proposed 'Health Education' subject in schools in Poland - attitudes, curriculum familiarity, and information sources.","authors":"Barbara Kołłątaj, Witold Piotr Kołłątaj, Justyna Wasiewicz, Agata Bryła Agata, Katarzyna Kłosowska, Wiktoria Kosek, Julia Justyna Soboń, Oktawia Maria Więckowska, Irena Dorota Karwat","doi":"10.26444/aaem/225377","DOIUrl":"https://doi.org/10.26444/aaem/225377","url":null,"abstract":"Introduction and objective: Public attitudes may affect implementation of school-based health promotion. In Poland, the proposed new subject 'Health Education' has been widely debated. The aim of the study is to assesses public response to this reform, including perceived health-related knowledge, curriculum evaluation and familiarity, reported information sources, and implementation preferences.Material and methods: A cross-sectional survey was conducted in 2025 among 1,094 adults from three Polish regions. A 32-item questionnaire assessed self-reported health-related knowledge, attitudes toward the reform, curriculum evaluation and familiarity, information sources, and implementation preferences. Descriptive statistics, Pearson's chi-square test, and Cramér's V were used.Results: Most respondents assessed their health-related knowledge positively, whereas evaluations concerning children and adolescents were less favourable. Overall, 51.9% supported changes in health education. Familiarity with the proposed curriculum was limited, with 57.3% reporting no or only superficial familiarity. Despite this, attitudes toward the proposed curriculum remained clearly divided, while 33.7% expressed no opinion. The most frequently preferred option was introducing the subject as optional.Conclusions: Although respondents generally recognized the need for changes in health education, attitudes toward the proposed curriculum remained divided despite limited familiarity with its content. Since most respondents indicated indirect or non-official information sources, declared opinions should be interpreted with caution, as the credibility, completeness, and fidelity of these sources to the official curriculum could not be verified.","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"373-378"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of anaesthesia levels monitored by Narcotrend on haemodynamics and postoperative cognitive function of elderly patients. 麻醉水平监测对老年患者血流动力学及术后认知功能的影响。

IF 1.2

Annals of agricultural and environmental medicine : AAEM Pub Date : 2026-09-21 Epub Date: 2026-04-03 DOI: 10.26444/aaem/218102

Ruina Guo, Xueli Zhao, Xizhong Ma, Jianghong Liu, Zhixun Hu

{"title":"The influence of anaesthesia levels monitored by Narcotrend on haemodynamics and postoperative cognitive function of elderly patients.","authors":"Ruina Guo, Xueli Zhao, Xizhong Ma, Jianghong Liu, Zhixun Hu","doi":"10.26444/aaem/218102","DOIUrl":"10.26444/aaem/218102","url":null,"abstract":"Objective: The aim of the study is to determine the appropriate level of anaesthesia for elderly patients when using Narcotrend for maintaining anaesthesia during surgery.Material and methods: A total of 135 elderly patients with intestinal tumours were randomly divided into 3 groups: A, B and C (during the surgery, the anaesthesia levels were maintained at D0, D2, and E1, respectively). Heart rate (HR) and Mean arterial pressure (MAP) were monitored at the following time points: T1 - before anaesthesia, T2 - before intubation, T3 - after intubation, T4 - during surgery, T5 - at the end of surgery, and T6 - at extubation. Additionally, cognitive function was evaluated through MMSE scores before and after the surgery.Results: Compared with T1, group A showed a significant acceleration in HR at T3, T5 and T6. However, no significant fluctuations in HR were observed in Group B and Group C. Furthermore, in T2 and T4, the MAP of all groups decreased, and the change was most significant in group C. After the surgery, the MMSE scores of Group B and Group C were higher. However, compared with group C, the dosage of anaesthetic drugs in group B was lower and the extubation time was shorter.Conclusions: Compared with D0, maintaining the anaesthesia level at D2 or E1 may be able to reduce the risk of postoperative cognitive dysfunction in elderly patients. Furthermore, compared to E1, the D2 level is more likely to maintain haemodynamic stability.","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"353-358"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial hazards in rainwater harvesting systems: bacterial sources, indicators, and health implications - a review. 雨水收集系统中的微生物危害：细菌来源、指标和健康影响综述。

IF 1.2

Annals of agricultural and environmental medicine : AAEM Pub Date : 2026-09-21 Epub Date: 2026-09-15 DOI: 10.26444/aaem/235841

Konrad Jamka, Grzegorz Raszewski, Grzegorz Łagód

{"title":"Microbial hazards in rainwater harvesting systems: bacterial sources, indicators, and health implications - a review.","authors":"Konrad Jamka, Grzegorz Raszewski, Grzegorz Łagód","doi":"10.26444/aaem/235841","DOIUrl":"https://doi.org/10.26444/aaem/235841","url":null,"abstract":"Introduction and objective: Rainwater harvesting systems (RWHS) are an important element of sustainable water management in urbanized areas, by supporting retention and adaptation to climate change. The widespread use of RWHS, however, is limited due to concerns about the microbiological purity of the water. The aim of the review is to illustrate the current state of knowledge regarding microbiological hazards and methods of their assessment in the rainwater of RWHS.Review methods: A comprehensive literature search was conducted using Scopus, ScienceDirect, and PubMed databases. Publications from 2000-2026 were analyzed, with special consideration of the studies published after 2015. Key words included: rainwater harvesting, RWHS, microbiological contamination, faecal indicator bacteria, FIB, microbial source tracking, MST, quantitative microbial risk assessment, QMRA.Brief description of the state of knowledge: RWHS systems are an effective tool for rainwater management and protection of its resources in integrated water resource management systems. However, the fundamental limitation to their broader implementation is mainly microbiological hazards, effectively limiting the use of rainwater for domestic and consumption purposes.Summary: The review organizes the knowledge on the safety of water from RWHS, considers microbiological hazards, FIB indicator bacteria, markers for identification of sources of microbiological contamination (MST), and QMRA microbiological risk assessment methods. It also discusses the limitations associated with FIB, the significance of MST, and the role of QMRA. Current data indicate that untreated water from RWHS is often suitable for use in non-consumption activities; however, it usually exceeds the standards set for drinking water. The water from RWHS intended for drinking most often requires treatment and continuous monitoring.","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"305-312"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AKT1 and ferroptosis involved in the anti-cerebral infarction effect of Ginkgolide Injection. AKT1和铁下垂参与银杏内酯注射液抗脑梗死作用。

IF 1.2

Annals of agricultural and environmental medicine : AAEM Pub Date : 2026-09-21 Epub Date: 2026-06-11 DOI: 10.26444/aaem/220801

Lu Lv, Jun Wang, Haige Wang, Jianrui Lin, Jiaqi Ruan, Qianchuan Chen

{"title":"AKT1 and ferroptosis involved in the anti-cerebral infarction effect of Ginkgolide Injection.","authors":"Lu Lv, Jun Wang, Haige Wang, Jianrui Lin, Jiaqi Ruan, Qianchuan Chen","doi":"10.26444/aaem/220801","DOIUrl":"https://doi.org/10.26444/aaem/220801","url":null,"abstract":"Introduction and objective: Ferroptosis is essential for the occurrence and development of myocardial infarction. The aim of the study is to elucidate the molecular mechanism of Ginkgolide Injection against ischemic cerebral infarction (ICI), focusing on its multi-target regulation of ferroptosis via the AKT1 and NFE2L2.Material and methods: Human primary neurons and astrocytes were subjected to oxygen-glucose deprivation/reoxygenation (OGD/R) to mimic cerebral ischemia. Cells were treated with Ginkgolide Injection or transfected with AKT1-targeting siRNA. Targets of Ginkgolide Injection components against ICI-associated genes were retrieved from TCMSP, CTD, HERB, OMIM, and DisGeNET databases. A compound-target-disease network was constructed using Cytoscape, followed by PPI, GO, and KEGG analyses. Cell viability (CCK-8), lipid peroxidation assessment (C11-BODIPY581/591 staining), redox markers (GSH, ROS, MDA), and AKT1/Nrf2 signaling (RT-qPCR, Western blot) were analyzed.Results: Ginkgolide Injection restored viability in OGD/R-injured neurons and astrocytes. Bilobalide in Ginkgolide Injection targeted 22 genes; Ginkgolide A targeted 32 genes; Ginkgolide B targeted 48 genes; Ginkgolide C targeted 30 genes; Ginkgolide J targeted 7 genes. A total of 55 targets for Ginkgolide Injection were common with ICI-associated genes. Network pharmacology identified AKT1 and NFE2L2 as core targets of Ginkgolide Injection against ICI. Ginkgolide Injection suppressed ferroptosis (reduced PTGS2 mRNA and lipid peroxidation) and activated AKT1 signalling in OGD/R-injured neurons and astrocytes. AKT1 knockdown abolished Ginkgolide's anti-ferroptotic effects, confirming AKT1's regulatory role.Conclusions: Ginkgolide Injection mitigates cerebral infarction by co-activating AKT1 and NFE2L2 to suppress ferroptosis, highlighting its potential as a multi-target therapy for ischemic ICI.","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"318-324"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0