Annals of agricultural and environmental medicine : AAEM最新文献

{"title":"Criticism of the proposed subject 'Health Education' in schools in Poland - the role of socio-demographic, regional, and health knowledge factors.","authors":"Barbara Kołłątaj, Witold Piotr Kołłątaj, Justyna Wasiewicz, Agata Bryła, Katarzyna Kłosowska, Wiktoria Kosek, Julia Soboń, Oktawia Maria Więckowska, Irena Dorota Karwat","doi":"10.26444/aaem/235733","DOIUrl":"https://doi.org/10.26444/aaem/235733","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Public attitudes may affect the implementation of school health reforms. The aim of the study is to examine whether perceived reasons for public criticism of Poland's proposed Health Education curriculum varied by socio-demographic, regional, educational, and health-knowledge characteristics.</p><p><strong>Material and methods: </strong>A 2025 cross-sectional survey included 1,094 adults recruited by convenience sampling in three provinces. Analyses included 1,046 respondents who declared at least some familiarity with the proposal. In a single-choice item, respondents selected one of four categories of reasons for public criticism: substantive, political, ideological, or other. Associations were tested using Pearson's chi-square test and Cramér's V, while age was compared using the Kruskal-Wallis test.</p><p><strong>Results: </strong>Perceived reasons for public criticism varied by place of residence, province, educational attainment, and health-knowledge level; all effect sizes were small. Substantive responses were more frequent among respondents with higher education than among those with secondary or vocational education, and among respondents with higher health-knowledge levels. Age, household size, self-rated health, and status as a parent or legal guardian of a school-age child, were not significantly associated with the selected category.</p><p><strong>Conclusions: </strong>Province showed the strongest association with respondents' perceptions, although the effect size remained small. Substantive explanations were more frequent in Mazowieckie and political explanations in Lubelskie, whereas ideological explanations were more frequent in Podkarpackie than in the other two provinces. Place of residence, educational attainment, and health-knowledge level showed weaker associations, suggesting that no single factor examined adequately accounts for the observed variation.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"379-384"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulation-based training to enhance emergency response in low-resource settings - a model for paramedic education in Ghana.","authors":"Ewa Zieliński, Katarzyna Wojtysiak, Karolina Osowiecka, Agnieszka Pedrycz, Mansur Rahnama-Hezavah, Paulina Mertowska, Sebastian Mertowski, Ewelina Grywalska, Anna Grzywacz, Marek Kowalczyk","doi":"10.26444/aaem/216872","DOIUrl":"https://doi.org/10.26444/aaem/216872","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Despite targeted investments in acute care in Ghana, significant challenges persist, including limited medical supplies, shortages of trained personnel, and restricted access to medications. A key barrier to effective prehospital care is the lack of formal education among paramedics. To address this gap, the Paramedics for Africa 3 programme was implemented between July - December 2020, aimed at improving emergency medical knowledge and skills among healthcare providers.</p><p><strong>Material and methods: </strong>A pre-post intervention design was used to evaluate the impact of the training. A total of 268 participants, including paramedics, hospital staff, and outpatient clinic workers, were enrolled. Each completed a 50-question multiple-choice test covering anatomy, diagnostics, and medical procedures. The programme included hands-on activities, simulation-based scenarios, and case-based learning. Statistical analysis was used to assess changes in test performance.</p><p><strong>Results: </strong>Baseline test scores ranged from 14% - 76% (median: 40%), increasing post-training to 32% -100% (median: 76%; p < 0.001). The most notable improvements were observed in trauma care and resuscitation. Emergency service staff demonstrated the most significant gains (p = 0.03). No statistically significant differences were found in terms of age, gender, education, or residence.</p><p><strong>Conclusions: </strong>The programme led to a significant improvement in participants' knowledge of emergency medicine. The positive impact of simulation-based learning highlights the importance of expanding such educational models in resource-limited healthcare settings.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"393-399"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-3615 suppresses proliferation and induces apoptosis in lung adenocarcinoma cells by targetting CALML4.","authors":"Ruijie Cao, Hao Xu, Zhong Guo, Yuxing Chen","doi":"10.26444/aaem/219015","DOIUrl":"https://doi.org/10.26444/aaem/219015","url":null,"abstract":"<p><strong>Introduction and objective: </strong>MicroRNAs (miRNAs) are essential in the modulation of cellular activities. Studies suggest that miR-3615 may possess a tumour-suppressive function in lung adenocarcinoma (LUAD); however, its precise role and molecular mechanisms in LUAD progression remain incompletely understood. The aim of the study is to systematically investigate the biological functions and underlying mechanisms of miR-3615 in LUAD.</p><p><strong>Material and methods: </strong>The expression of miR-3615 in LUAD cell lines was quantified by real-time quantitative PCR (RT-qPCR). Subsequently, its functional effects on proliferation, migration, invasion, and apoptosis were evaluated in A549 and H1299 cells using the CCK-8 assay, Transwell assay, and flow cytometry, respectively. Potential target genes of miR-3615 were predicted and functionally annotated using bioinformatics methods. The binding interaction of miR-3615 with the CALML4 gene was demonstrated via a dual-luciferase reporter assay.</p><p><strong>Results: </strong>miR-3615 expression was notably downregulated in LUAD cell lines compared with normal cells. Overexpression of miR-3615 inhibited the proliferation, migration, and invasion capabilities of LUAD cells while promoting apoptosis. Integrated bioinformatics analysis involving target prediction and functional enrichment identified CALML4 as a key candidate target gene of miR-3615. CALML4 was confirmed as a direct target of miR-3615. The effects of miR-3615 mimic on LUAD cell proliferation, migration, invasion, and apoptosis were partly attenuated by CALML4 overexpression.</p><p><strong>Conclusions: </strong>miR-3615 exerts its functional impact on LUAD cells by directly downregulating CALML4, thereby curbing proliferation, migration, and invasion and promoting apoptosis. The elucidated miR-3615/CALML4 axis advances the theoretical foundation for studying LUAD development.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"418-424"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The function of miR-4666a-3p in the diagnosis of osteoporotic fractures and the promotion of fracture healing.","authors":"Xiangnan Zou, Zhongshu Ren, Haitao Xu, Hao Wang","doi":"10.26444/aaem/220802","DOIUrl":"https://doi.org/10.26444/aaem/220802","url":null,"abstract":"<p><strong>Introduction and objective: </strong>MicroRNAs (miRNAs) regulate osteogenic differentiation in osteoporotic fractures (OF). The aim of the study is to investigate the diagnostic value and regulatory role of miR-4666a-3p in OF.</p><p><strong>Material and methods: </strong>The study is based on clinical samples (89 healthy controls, 79 OP patients, 83 OF patients) and hFOB1.19 cell experiments. RT-qPCR analyzed miR-4666a-3p expression, and logistic regression identified OF risk factors. ELISA measured RANKL and OPG concentrations. In vitro, osteoblast differentiation was induced to assess ALP activity, Runx2, Osteocalcin, Col1a1, cell viability (CCK-8), and apoptosis (flow cytometry). Target genes were bioinformatically predicted and confirmed via dual-luciferase assay. Rescue experiments explored the role of GSK3B in miR-4666a-3p-mediated osteogenic regulation. All experiments were repeated at least three times.</p><p><strong>Results: </strong>Downregulation of miR-4666a-3p could distinguish healthy controls and OP patients, further differentiate between OP and OF patients, and may predict the risk of OF occurrence. During osteoblast differentiation, miR-4666a-3p expression increased, ALP activity elevated, and key marker levels upregulated. Overexpression of miR-4666a-3p promoted osteoblast differentiation and activity, and reduced apoptosis rate, whereas inhibiting miR-4666a-3p had the opposite effect. MiR-4666a-3p negatively regulated the downstream target GSK3B. Overexpression of GSK3B reversed the osteogenic effects of miR-4666a-3p.</p><p><strong>Conclusions: </strong>Downregulation of miR-4666a-3p may predict OF risk. Its upregulation promoted osteoblast differentiation by targeting GSK3B, potentially facilitating fracture healing.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"453-462"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizoma Atractylodis Macrocephalae (Atractylodes Macrocephala Koidz.) ameliorates asthma via inhibiting TNF-α.","authors":"Jinling Luan, Xiaoyan Su, Na Chen","doi":"10.26444/aaem/217032","DOIUrl":"https://doi.org/10.26444/aaem/217032","url":null,"abstract":"<p><strong>Objective: </strong>The aim of thev study is to investigate the therapeutic effects and underlying mechanisms of RAM in a house dust mite (HDM)-induced murine model of allergic asthma, with a focus on necroptosis and TNF signaling.</p><p><strong>Material and methods: </strong>Allergic asthma was induced in mice and HBE cells by HDM challenge. Animals were treated with RAM to assess its effects on airway inflammation, hyperresponsiveness, IgE production, and cytokine/chemokine expression. In vitro, necroptosis markers (RIPK1, RIPK3, p-MLKL), inflammatory cytokines, and cell viability were evaluated in HBE cells. Network pharmacology and molecular docking were employed to predict RAM's bioactive compounds and their primary targets, with a focus on the necroptosis pathway. The role of TNF was further validated through overexpression experiments in HBE cells.</p><p><strong>Results: </strong>RAM treatment significantly alleviated asthma phenotypes, reducing inflammatory cell infiltration in BALF, serum IgE levels, airway hyperresponsiveness, and pulmonary expression of Cxcl1/Cxcl2. RAM suppressed expression of Ripk1, Ripk3, p-MLKL, and caspase-3, alongside reduced proinflammatory cytokines (IL-1α, IL-1β, IL-33), in HDM-induced mice and HBE cells. Network pharmacology identified TNF as a top-ranked target within the necroptosis pathway, and molecular docking confirmed binding affinities between TNF and five RAM compounds. Experimentally, RAM downregulated TNF expression in mouse lungs and HBE cells. TNF overexpression reversed RAM's protective effects, restoring Ripk1/Ripk3 expression and diminishing cell viability.</p><p><strong>Conclusions: </strong>RAM may attenuate allergic asthma by inhibiting the TNF-mediated necroptosis pathway. This study provides a pharmacological basis for RAM as a promising therapeutic agent for asthma treatment.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"400-406"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LINC00861 protects against sepsis-associated acute kidney injury by regulating apoptosis and inflammation via miR-151a-3p/PDHA1 Axis.","authors":"Xinghua Li, Debiao Yu, Dongqin Shen, Yeting Zhang, Wei Wang","doi":"10.26444/aaem/219991","DOIUrl":"https://doi.org/10.26444/aaem/219991","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Sepsis-associated acute kidney injury (SA-AKI) is a critical complication of sepsis marked by high morbidity and mortality. The aim of the study is to investigate the role of LINC00861 and its downstream regulatory axis in SA-AKI.</p><p><strong>Material and methods: </strong>220 SA-AKI patients were enrolled and stratified according to survival outcomes. The levels of LINC00861, miR-151a-3p, and PDHA1 were detected in peripheral blood, and correlations with clinical parameters and prognosis were analyzed. <i>In vitro</i>, HK-2 cells were subjected to LPS to establish an SA-AKI model. Functional assays, including flow cytometry, ELISA, and qRT-PCR, were performed to evaluate apoptosis and inflammatory cytokines (IL-6, IL-18, TNF-α). Dual-luciferase assays and rescue experiments were conducted to elucidate the regulatory relationship among LINC00861, miR-151a-3p, and PDHA1.</p><p><strong>Results: </strong>LINC00861 expression was reduced in SA-AKI non-survivor patients and was associated with higher SOFA and APACHE II scores, elevated PCT, SCr, and lactate levels. ROC analysis demonstrated good diagnostic performance (AUC = 0.835). LINC00861 (HR = 0.31, 95% CI: 0.16-0.58) was recognized as an independent prognostic factor for SA-AKI. Overexpression of LINC00861 suppressed LPS-induced apoptosis and reduced pro-inflammatory cytokine release in HK-2 cells. LINC00861 functioned as a sponge for miR-151a-3p, showing a negative correlation. miR-151a-3p directly targeted PDHA1, which was downregulated in non-survivors and positively correlated with LINC00861. Mechanistic analyses revealed that LINC00861 manages apoptosis and inflammation response by the miR-151a-3p/PDHA1 axis.</p><p><strong>Conclusions: </strong>Reduced LINC00861 was independently associated with poor prognosis in SA-AKI. In vitro, LINC00861 was shown to regulate apoptosis and inflammation through the miR-151a-3p/PDHA1 axis.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 3","pages":"439-445"},"PeriodicalIF":1.2,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149070781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cytoprotective role of whey protein hydrolysates against CdCl₂-induced toxicity in fibroblasts by the Electric Cell-substrate Impedance Sensing (ECIS) method.","authors":"Dominika Pigoń-Zając, Hussein B Khudhur, Jose L Valverde Piedra, Teresa Małecka-Massalska, Monika Prendecka-Wróbel","doi":"10.26444/aaem/208051","DOIUrl":"https://doi.org/10.26444/aaem/208051","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Cadmium (Cd) is a toxic mineral element with a long half-life that causes excessive accumulation in tissues, especially the kidney. It influences cell development, proliferation, and death by activating intracellular signalling pathways. Currently, treatment options include chelation agents and antioxidant therapy. Researchers are investigating natural compounds that might protect against Cd-induced toxicity while providing less negative effects. The aim of the study is to look into the protective properties of bovine milk whey protein hydrolysates against Cd-induced toxicity in in vitro studies.</p><p><strong>Material and methods: </strong>The study was performed by testing different concentrations of CdCl₂ on fibroblast cell culture after the addition of different concentrations of whey peptides using the Electric Cell-substrate Impedance Sensing (ECIS) assay, in order to study intermediate-term exposure (20-25 hrs).</p><p><strong>Results: </strong>By using the ECIS test, it was found that the higher the CdCl₂ concentration, the lower the impedance value, which indicates a decrease in cell viability. At the same time, it was found that the use of whey proteins in concentrations of 0.5%, 0.8% and 1.2% had a protective effect on cells treated with toxic cadmium. It could be stated that protein peptides present in the medium may be responsible for binding cadmium, preventing its interaction with the fibroblast cell membrane and cell organelles.</p><p><strong>Conclusions: </strong>The study provided evidence that bovine whey proteins have a protective effect against CdCl₂ toxicity. This could be due to its enhanced antioxidant defence and metal chelating properties; therefore, whey proteins could be a useful nutritional supplement to reduce Cd toxicity.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 2","pages":"271-280"},"PeriodicalIF":1.2,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atypical eczema-like skin symptoms and joint swelling in a patient with Toxocara seropositivity - Case Report.","authors":"Łukasz Augustyn Pielok, Arleta Kowala-Piaskowska, Olgierd Kłodziński","doi":"10.26444/aaem/209623","DOIUrl":"https://doi.org/10.26444/aaem/209623","url":null,"abstract":"<p><strong>Introduction: </strong>Toxocariasis is the most widespread zoonotic parasitic infection, shared between humans, dogs, and wild canids. Clinical manifestations vary widely, often leading to delayed diagnosis, with allergic skin reactions and joint swelling being less common.</p><p><strong>Case report: </strong>A 53-year-old man presented wityh unusual allergic skin symptoms, including a rash, blisters, and joint swelling. The symptoms occurred intermittently over several years.</p><p><strong>Results: </strong>Extensive testing yielded negative results, with the exception of anti-Toxocara antibodies, suggesting that Toxocara infection may have contributed to the symptoms or triggered an inflammatory response in the host. Albendazole therapy was initiated, resulting in an improvement in the skin symptoms.</p><p><strong>Conclusions: </strong>This cosmopolitan parasitic disease should always be considered in the diagnosis of allergic skin changes and joint swelling. Serological testing plays a crucial role in the diagnostics.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 2","pages":"290-292"},"PeriodicalIF":1.2,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of <i>Thermobifida fusca</i> as a main causative agent of mushroom compost worker's lung - case series.","authors":"Barbara Mackiewicz, Angelina Wójcik-Fatla, Jacek Dutkiewicz, Jan Siwiec, Tomasz Kucharczyk, Elżbieta Czekajska-Chebab, Mariola Paściak, Bogumiła Szponar, Grażyna Cholewa, Janusz Milanowski","doi":"10.26444/aaem/224343","DOIUrl":"https://doi.org/10.26444/aaem/224343","url":null,"abstract":"<p><strong>Introduction and objective: </strong>Mushroom compost worker's lung (MCWL) is a hypersensitivity pneumonitis (HP) entity occurring in workers employed at preparing compost for the cultivation of white button mushrooms. The aim of the study is to identify the strains of thermophilic actinobacteria as the potential causative agents of MCWL symptoms in workers.</p><p><strong>Material and methods: </strong>Clinical examinations included chest X-ray, high-resolution computed tomography (HRCT), bronchoscopy with bronchoalveolar lavage (BAL), pulmonary function tests comprising spirometric measurements, DLCO measurements, body pletysmography, and arterial - blood gas tests. Identification of thermophilic actinobacteria was performed by microscopy, culture and genetic methods. Allergological tests included the agar gel precipitation test and inhalation challenge with actinomycetal antigens.</p><p><strong>Results: </strong>Among a group of 20 patients, 15 showed in HRCT ground-glass attenuations solely and were diagnosed with acute HP, 3 showed ground-glass attenuations with pulmonary fibrosis and were diagnosed with subacute HP, while 2 patients were diagnosed with Organic Dust Toxic Syndrome (ODTS). <i>Thermobifida fusca</i> and <i>Laceyella sacchari</i> strains were isolated from the symptom-causing mushroom compost. In 3 workers, strong precipitin reactions to <i>Th. fusca</i> were found, whereas there were no reactions to <i>L. sacchari</i>. Four workers showed a positive inhalation response to small doses of <i>Th. fusca</i> antigen.</p><p><strong>Conclusions: </strong>The results of this study indicate that <i>Th. fusca</i> is most probably a major causative agent of MCWL and should be considered in the diagnosis of HP in workers of the mushroom compost facility.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 2","pages":"183-191"},"PeriodicalIF":1.2,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Family medicine in rural and depopulating recommendations in the light of the professional preferences of young physicians.","authors":"Magdalena Łoś, Aneta Nitsch-Osuch, Paweł Żuk, Artur Prusaczyk, Jakub Owoc, Andrzej Silczuk, Aleksander Biesiada, Jerzy Kuliński, Monika Kaczoruk","doi":"10.26444/aaem/219136","DOIUrl":"https://doi.org/10.26444/aaem/219136","url":null,"abstract":"<p><p>An important factor in the effective use of basic healthcare services is spatial accessibility, the lack of which contributes to health inequalities and social exclusion. The phenomenon of medical 'deserts' illustrates the complex processes resulting from the overlap of socio-demographic and epidemiological conditions. The subject of the study was expert opinions on the development of targeted solutions aimed at reducing the phenomenon of medical deserts and work in depopulated areas in Poland. To reverse the trends in the career preferences of young medical professionals, action is required at various levels. A key factor, according to experts, is completing a postgraduate internship. Through various types of short- and long-term placements, internships, and rotations, medical students come into contact with a wide range of mentors and professional role models. Multifaceted cooperation is important in implementing health policy tasks to help reduce the phenomenon of medical deserts.</p>","PeriodicalId":520557,"journal":{"name":"Annals of agricultural and environmental medicine : AAEM","volume":"33 2","pages":"281-289"},"PeriodicalIF":1.2,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}