{"title":"Adipose-derived stem cells and ginkgo biloba extract-loaded PCL/gelatin nanofibrous scaffolds for peripheral nerve injury repair: the impact of physical activity.","authors":"Hua Gong, Zhengtian Chen","doi":"10.37190/abb-02323-2023-06","DOIUrl":"https://doi.org/10.37190/abb-02323-2023-06","url":null,"abstract":"<p><p>Peripheral nerve damages take place as a result of trauma, compression or disease, resulting in sensory loss, impaired motor function and subsequent challenges. In the current study, ginkgo biloba extract was loaded into PCL/gelatin scaffolds through electrospinning method. The scaffolds were characterized <i>in vitro</i> using various studies. The prepared nanofibrous scaffolds were rolled up to make neural guidance channels. Then, the conduits were seeded with adipose-derived stem cells and transplanted into a rat model of sciatic nerve injury. The scaffolds were not toxic and had optimal tensile and suturability. The animals treated with the conduits that delivered adipose-derived stem cells and ginkgo biloba extract, and received the treadmill exercise, had significantly higher motor and sensory functions recovery. In addition, histopathological examinations showed beneficial role of the exercise plan on the nervous system repair.</p>","PeriodicalId":519996,"journal":{"name":"Acta of bioengineering and biomechanics","volume":"25 4","pages":"35-47"},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of wearing high-heeled shoes on postural control and foot loading symmetry.","authors":"Michalina Błażkiewicz, Anna Hadamus","doi":"10.37190/abb-02337-2023-02","DOIUrl":"https://doi.org/10.37190/abb-02337-2023-02","url":null,"abstract":"<p><p><i>Purpose</i>: This study aimed to evaluate the effects of high-heeled shoes (HHS) and experience with such footwear on foot loading and standing balance using linear and nonlinear methods. <i>Methods</i>: Sixteen young female experts in wearing high-heeled shoes (HHE) and sixteen young females who occasionally wore high-heeled shoes (HHO) completed a Fall Risk Test (FRT) on the Biodex Balance System platform. They also underwent a both-leg standing test on the Zebris pressure mapping platform, both barefoot and while wearing 11 cm HHS. The study analyzed several parameters, including the FRT index, foot loading parameters, linear measures of postural stability (Center of Pressure (CoP) path length and velocity), and nonlinear postural control measures (sample entropy - SampEn, fractal dimension - FD, and the largest Lyapunov exponent - LyE). <i>Results</i>: HHS caused a significant increase the fall risk of more than 44%, but only in the HHE group. The presence of HHS caused a significant increase in CoP path length and CoP velocity by almost 78%. The values of these parameters increased by more than 67% in the HHO group and by more than 92% in the HHE group. HHS caused a significant increase in the values of nonlinear measures (FD and LyE) in the mediolateral direction. Higher FD and LyE values suggest the ability to react faster to destabilizing stimuli and better balance control related to plasticity and adaptability to new conditions. HHS also led to up to 70% loading on the supporting limb. <i>Conclusions</i>: High heels in the population of young women significantly worsen static balance.</p>","PeriodicalId":519996,"journal":{"name":"Acta of bioengineering and biomechanics","volume":"25 4","pages":"49-57"},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Analysis of segmental coordination in the lower extremity using vector coding: a pilot return-to-play study of acute ankle sprain.","authors":"Xiaoyi Yang, Hanhui Jiang, Peimin Yu, Qichang Mei, Justin Fernandez, Yaodong Gu","doi":"10.37190/abb-02363-2023-02","DOIUrl":"https://doi.org/10.37190/abb-02363-2023-02","url":null,"abstract":"<p><p><i>Purpose</i>: Acute ankle sprain may affect ankle function during sport and daily activities. This study aimed to use vector coding technique to analyze the difference over time between injured and healthy lower limb during the first week of acute ankle sprain phase (P1) and post a 1-month recovery phase (P2) to understand the return-to-play coordination strategy in the lower extremity. <i>Methods</i>: Six females attended the gait experiments with attached 40 reflective markers using eight-camera Vicon motion capture system. All participants walked barefoot while turning in four directions (T0°, T45°, T90°, T135°) at their self-selected speed. Coordination patterns were classified as in-phase, anti-phase, proximal or distal dominancy between lower limb joints involving hip, knee, ankle, subtalar, metatarsophalangeal (MTP) joint and tarsometatarsal (TMT) joint. <i>Results</i>: P1 showed more proximal joint dominant in Hip-Knee coupling angles but P2 displayed more distal joint dominant in Knee-Ankle joint coordination pattern and mainly distal joint dominant in Ankle-MTP coupling angle mapping. The Ankle-TMT1 and Ankle-TMT5 coordination patterns matched best in straight walking but worst in T135 walking. <i>Conclusions</i>: Investigating inter-segmental coordination in different turning movements could provide insights into gait changes from acute ankle sprain from one-month return-to-play recovery. Knowledge of lower limb coordination pattern may provide clinical implications to improve dynamic balance and gait stability for individuals with acute ankle sprain.</p>","PeriodicalId":519996,"journal":{"name":"Acta of bioengineering and biomechanics","volume":"25 4","pages":"23-34"},"PeriodicalIF":0.0,"publicationDate":"2024-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Piotr Gruber, Patrycja Szymczyk-Ziółkowska, Michał Olejarczyk, Adam Junka, Krystyna Fabianowska-Majewska, Malwina Brożyna, Tomasz Kurzynowski
{"title":"Influence of bioactive metal fillers on antimicrobial properties of PA12 composites produced by laser-based powder bed fusion of polymers.","authors":"Piotr Gruber, Patrycja Szymczyk-Ziółkowska, Michał Olejarczyk, Adam Junka, Krystyna Fabianowska-Majewska, Malwina Brożyna, Tomasz Kurzynowski","doi":"10.37190/abb-02303-2023-04","DOIUrl":"https://doi.org/10.37190/abb-02303-2023-04","url":null,"abstract":"<p><p><i>Purpose</i>: This study investigated the influence of three types of metallic microfillers, spherical silver and spherical, and dendritic copper, on the ability of polyamide 12 (PA12) to inhibit microorganism growth on the surfaces of samples produced using laser-based powder bed fusion of polymers (PBF-LB/P). The aim of this study was to initially characterize these materials regarding their potential applicability for parts dedicated to use in the hospitals, where surfaces are periodically disinfected using chemical and/or physical measures. <i>Methods</i>: Composite powders with filler concentrations of 0.5, 1, 2 and 5% by weight were prepared using the mechanical mixing method and processed using PBF-LB/P. Three common hospital pathogens responsible for healthcare-associated infections: <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i> and <i>Candida albicans</i> were tested. Additionally, the safety of the composites was assessed through <i>in vitro</i> tests using human cell lines: keratinocytes and fibroblasts. <i>Results</i>: The research reveals that addition of copper or silver causes decrease in bacterial colony viability compared to the material without a filler, but an insignificant effect on antifungal properties. There was no significant impact within the tested range of filler's content on the antibacterial properties. Furthermore, a strong effect of the microfillers on tested material's toxicity is observed. <i>Conclusions</i>: The addition of metallic microfillers enhances the antibacterial response of polymeric materials processed with PBF-LB/P. Nevertheless, the observed varying levels of cytotoxicity toward eukaryotic cell lines underscore the need for further studies on the analysed materials to unequivocally determine their potential applicability as materials for short-term contact with human skin in a hospital setting.</p>","PeriodicalId":519996,"journal":{"name":"Acta of bioengineering and biomechanics","volume":"25 4","pages":"11-21"},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}