BioMedicine-Taiwan最新文献

{"title":"Herpes simplex virus: A case report and review of literature","authors":"Mamatha Shetty, Deepa Sara John, S. Durgapal, U. Shetty","doi":"10.51248/.v43i3.2099","DOIUrl":"https://doi.org/10.51248/.v43i3.2099","url":null,"abstract":"Herpes simplex virus (HSV) is a double-stranded DNA virus that exists in two forms- HSV 1 and HSV 2. Oral herpetic lesions are typically caused by HSV-1. In the oral cavity, they are known to cause painful lesions that most commonly involve gingiva, palate, and buccal mucosa. Since it is a contagious infection and has the potential to cause complications in the host, timely diagnosis and management are important. Therefore, dental professionals must be aware of clinical presentation and appropriate diagnostic and management measures. The following case report discusses a case of Herpes Simplex Virus in a 33-year-old, systemically healthy female patient, who reported to the dental OPD with oral symptoms. The clinical features, investigations, diagnosis, and management of the condition have been elaborated in the review.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44864466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical analysis, in-vitro antioxidant and cytotoxicity activities of Flaveria trinervia ethanolic extract","authors":"Anju A. Varghese, A. Smith A., S. M.","doi":"10.51248/.v43i3.2526","DOIUrl":"https://doi.org/10.51248/.v43i3.2526","url":null,"abstract":"Introduction and Aim: Crude herbal drugs and commercial extracts obtained from plant sources continue to play a significant role in healthcare, particularly in developing countries where traditional medicine practices are deeply rooted. The aim of the study was to estimate the antioxidant (DPPH) and cytotoxic effects of the ethanolic extract of Flaveria trinervia on raw 246.7 cells.\u0000 \u0000Materials and Methods: In the Indian town of Thoothukudi's Kovilpatti village, F. trinervia was isolated. The entire plant was air-dried before being crushed, and 1 kg of the powdered ethanolic extract was employed in the Soxhlet apparatus for continuous extraction. By utilizing GC-MS analysis, F. trinervia ethanolic extract phytochemical assessment was studied. The antioxidant potential of F. trinervia was examined using the DPPH test. Raw 246.7 cell lines were used to test the ethanolic extract of F. trinervia for cytotoxic activity.\u0000 \u0000Results: F. trinervia extracts were found to contain a variety of biologically active phytoconstituents, including ergosterol, octadecanoic acid, propanoic acid, and isopropyl palmitate, according to phytochemical analysis. Compared to the negative control, the ethanolic extract of F. trinervia considerably showed antioxidant activity and cytotoxicity. Ascorbic acid antioxidant activity with DPPH was 98.12%, whereas F.trinervia ethanolic extract's antioxidant activity was 78.91%. The anticancer efficacy of F. trinervia ethanolic extract against uncultured 246.7 cells was evaluated using the MTT assay. By charting cell viability vs extract concentration, the IC50 value was determined. The MTT assay identified raw cells IC50 at 24 hrs as 200 µg/mL of ethanolic F. trinervia extract.\u0000 \u0000Conclusion: Active phytoconstituents are present in the F. trinervia whole plant ethanolic extract. Because of the presence of phytoconstituents, the extracts have excellent antioxidant activity and good cytotoxicity activity against raw 246.7 cell lines. The study recommended using whole plant extract from F. trinervia to treat several metabolic diseases.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45205430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changing trends in blood component utilisation during COVID time: Analysis of 1096 COVID-19 in patients","authors":"Christy K. Jose, K. Pailoor","doi":"10.51248/.v43i3.1334","DOIUrl":"https://doi.org/10.51248/.v43i3.1334","url":null,"abstract":"Introduction and Aim: In the global pandemic caused by Covid-19, older individuals and those with comorbidities have been shown to be more susceptible to serious diseases. The purpose of our research is to estimate the proportion of Covid-19 inpatients requiring blood transfusions over a period of 3 months.\u0000 \u0000Materials and Methods: A retrospective observational analysis of blood product consumption in Covid-19 inpatients at our center from April to June 2021 was conducted by reviewing monthly transfusion records archived in our blood bank.  Each blood component administered in Covid-19 wards during the prescribed period was noted.\u0000 \u0000Results: Out of 1096 Covid-19 inpatients, only 66 (6.02%) patients received transfusions. As compared to non-Covid wards, Covid-19 wards had substantially lower transfusion rates for packed cell units (84 vs. 1074 units), FFP (33 vs. 691 units), and platelets (46 vs. 595 units). A total of 163 units of blood components were given to 66 Covid-19 inpatients, the majority of which were packed cell units (51.5%).\u0000 \u0000Conclusion: In comparison to hospitalized non Covid patients, Covid-19 inpatients required fewer transfusions. In our institute, only the Covid 19 patients who were critically sick received blood transfusions; the rest received conservative therapy for their mild to moderate illnesses. This information will be beneficial for our department as it helps us plan and prepare for the use of blood supplies while the pandemic is still going strong.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43293580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the role of zinc finger protein-804a (znf804a) (rs1344706) gene polymorphism in schizophrenia and methamphetamine addiction patients","authors":"Saja Mahir Mohammed, Zainab Hassan Hashim, Qasim Sh. Al-Mayah, Mahir Mohammed Hussein","doi":"10.51248/.v43i3.2819","DOIUrl":"https://doi.org/10.51248/.v43i3.2819","url":null,"abstract":"Introduction and Aim: The zinc finger protein 804A (ZNF804A) gene has garnered significant interest as a gene associated with an increased risk of psychosis. This interest stems from the strong association observed between a specific single nucleotide polymorphism (SNP), namely rs1344706, within the ZNF804A gene, and various psychosis phenotypes, including schizophrenia and bipolar disorder. In this study, we aimed to assess the functional impact of ZNF804A gene polymorphism in individuals diagnosed with schizophrenia and methamphetamine addiction.\u0000 \u0000Materials and Methods: This nested case control research included 50 individuals with schizophrenia and 50 methamphetamine (crystal) users recruited from Ibn-Rushed Psychiatric Teaching Hospital in Baghdad. 50 healthy subjects were also recruited as a control group. All participants were subjected to thorough physical examination. The serum concentration of Zinc Finger Protein-804A (ZNF804A) was determined using a sandwich enzyme-linked immunosorbent assay. Genomic DNA was isolated from whole blood samples, and the Taqman allelic discrimination method was used to genotype the ZNF804A gene rs1344706 polymorphism.\u0000 \u0000Results: Patients with schizophrenia had a significantly higher median level of ZNF804A (48.67 ng/L) than controls (25.56 ng/L) or methamphetamine users (35.62 ng/L). The SNP rs1344706 homozygous (AA) genotype was more common in schizophrenia patients (36% vs. 16%) and methamphetamine addiction patients (4%), with significant differences (OR= 1.32, 95%CI= 1.32-11.75, p=0.014 and OR= 11.7, 95%CI=02.43-56.4, p=0.002, respectively).\u0000 \u0000Conclusion:  The AA genotype of rs1344706 polymorphism could be considered a risk factor for schizophrenia but has a protective role against methamphetamine addiction. Serum level of ZNF804A could be used to confirm the diagnosis of schizophrenia in young individuals with subtle clinical features. ","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48721861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipoprotein lipase gene polymorphisms and their association with the development and severity of carotid artery stenosis","authors":"Mahmood Shaker Khazaal, Farqad Bader Hamdan, Qasim Sharhan Al-Mayah","doi":"10.51248/.v43i3.2818","DOIUrl":"https://doi.org/10.51248/.v43i3.2818","url":null,"abstract":"Introduction and Aim: In developed countries, carotid artery stenosis (CAS) has a considerable impact on mortality and disability rates. Genetic risk factors for CAS have also been linked, in addition to environmental risk factors. This study sought to determine whether there may be a link between three polymorphisms in the LPL gene and the emergence of CAS.\u0000 \u0000Materials and Methods: One hundred and twenty participants were enrolled in this case-control study, including 60 individuals with CAS and 60 healthy subjects serving as the control group. The demographic and clinical data were collected from each participant. Whole blood samples were obtained to study the genomic DNA where a specific LPL gene fragment corresponding to the three single nucleotide polymorphisms (SNPs) rs320, rs328, and rs285 was amplified using designated primers. Restriction fragment length polymorphism analysis was used for genotyping.\u0000 \u0000Results: The mutant allele (C) of the rs285 polymorphism was more frequent in patients than controls (45% vs. 32.5%; OR=1.7, 95% CI= 1.01- 2.87; p=0.048). The TCC haplotype block (T allele of rs320, C allele of rs285, and C allele of rs328) was significantly more prevalent in patients compared to controls (OR= 2.0, 95% CI= 1.03-3.77, p= 0.039). On the other hand, controls (23.33%) had significantly more of the haplotype block GTG than did patients (8.33%) (OR = 0.3, 95% CI = 0.14-0.065, p = 0.002). The SNPs rs320 (D' = 0.63) and rs328 (D' = 0.61) have weak relationships with rs385.\u0000 \u0000Conclusion: The C allele of rs285 polymorphism could be considered a risk factor for CAS. While the haplotype block GTG was thought to play a protective role, the haplotype block TCC (T allele of rs320, C allele of rs285, and C allele of rs328) may increase the risk of CAS.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49525582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of reactive oxygen species on the progression of human diseases by damaging biomolecules","authors":"V. Pathak, R. Kant, Navneeta R. Kumar","doi":"10.51248/.v43i3.2439","DOIUrl":"https://doi.org/10.51248/.v43i3.2439","url":null,"abstract":"Reactive oxygen species (ROS) are the molecules which have oxygen an atom in its highly reactive form. ROS are formed as a byproduct of normal metabolism and are removed by the antioxidants and enzymes present in the system. But, uncontrolled formation of ROS due to various factors like diseases or stress causes damage to the biomolecules. This oxidation of biomolecules by ROS might help in the progression of human diseases. Understanding the mechanism of development of different human diseases caused due to ROS will help to control the severity of the diseases. Also, on the basis of types of ROS involved, an antioxidant supplement can be selected to mitigate the effect of ROS and ultimately lower the severity of the disease. In this review article, we have tried to summarize the literature available from different sources on ROS formation, biomolecule oxidation and their impact on the progression of various diseases in humans.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44651411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of conventional management and Carica papaya on influencing wound healing: A systematic review and meta-analysis","authors":"Sathya Siva, Madhanraj Sekar, Surya Prakash V., Titus J., Mohammed Arifullah Mohamed Fazulah, S. V","doi":"10.51248/.v43i3.2851","DOIUrl":"https://doi.org/10.51248/.v43i3.2851","url":null,"abstract":"Protease enzymes found in the Indian papaya, also called Carica papaya, are known to have de-sloughing and wound healing properties. It was found that Carica papaya applied to a lesion enhanced phagocytic cell killing of bacteria. Green papaya is rich in papain and chymopapain, two potent digestive enzymes that can break down dead tissue and have anti-inflammatory properties. To compare the effectiveness of conventional and Carica papaya management in wound healing the systematic review and meta-analysis is conducted. Based on PRISMA (preferred reporting items for systematic reviews and meta-analyses) guidelines, a systematic review is conducted. While using the search terms ‘Diabetic foot ulcer, conventional management, wound healing, Carica papaya’ in the MEDLINE, Google Scholar, and PubMed databases. Carica papaya has a good beneficial effect on reducing necrosis and is frequently utilized in the management of wound healing. None of the studies have demonstrated any adverse impacts of Carica papaya on wound healing.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46613814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of silver nanoparticles on multiple drug-resistant strains of Staphylococcus aureus from periodontal infection: An alternative approach for antimicrobial therapy","authors":"Shaimaa Noori Mahal, Ahmed Mohammed Turki, Elham Hazeim Abdulkareem","doi":"10.51248/.v43i3.2822","DOIUrl":"https://doi.org/10.51248/.v43i3.2822","url":null,"abstract":"Introduction and Aim: Most cases of periodontitis are associated with microorganisms. The Gram positive bacteria Staphylococcus aureus is considered as one of the important organisms associated with periodontal infections. This study investigated the effect of silver nanoparticles as well as the antiseptic agent chlorhexidine on multi-drug resistant S. aureus isolated from periodontal infections.\u0000 \u0000Materials and Methods: In this study, with help from dentists, 266 clinical samples were collected from dental patients who had periodontal infection. S. aureus isolated from samples was tested for their antibiotic susceptibility profiles. Silver nanoparticles and chlorhexidine were evaluated for their antibacterial activity against these S. aureus isolates.\u0000 \u0000Results: S. aureus strains isolated from periodontal infection patients in this study were found to be multidrug resistant. AgNPs obtained using E. coli showed high inhibition of S. aureus growth when used in different concentrations (5, 10, 15, 20, 25mM). Chlorhexidine also exhibited antibacterial activity against S. aureus. Combination of AgNPs with penicillin and ciprofloxacin had an increasing significant effect on the sensitivity of S. aureus. Similarly, chlorhexidine in combination with penicillin and ciprofloxacin also showed an inhibitory effect on the growth of S. aureus.\u0000 \u0000Conclusion: AgNPs and chlorhexidine combined with antibiotics used in treatment of S. aureus isolated from periodontal disease showed a good antibacterial effect which suggests its use as an antibacterial agent against periodontitis associated bacteria.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43672611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do miR-146a plasma levels alter in Alzheimer’s patients and impact their cognitive functions?","authors":"Sadiruldeen Sami Abed, Farqad Bader Hamdan, Qasim Sharhan Al-Mayah, Mahir Mohammed Hussein","doi":"10.51248/.v43i3.2817","DOIUrl":"https://doi.org/10.51248/.v43i3.2817","url":null,"abstract":"Introduction and Aim: The miR-146a is a short non-coding RNA molecule that has both therapeutic and biomarker potential. Abnormal miR-146a expression has been linked to several disorders. The target of the study is to investigate the possible link between miRNA-146a expression and the deterioration of cognitive function in Alzheimer's patients.\u0000 \u0000Materials and Methods: The research comprised 40 individuals from Iraq, spanning both genders and ranging in age from 60 to 85 years. They were segregated into two distinct groups. The first group included 40 subjects (age of 75±6.6 years) who displayed no signs of cognitive or functional impairment. The second group consisted of 40 patients diagnosed with Alzheimer's disease (with an average age of 74.98±5.03 years), as per the DSM-5 criteria. To assess the mir-146 gene expression, quantitative polymerase chain reaction (qPCR) was employed.\u0000 \u0000Results: The median expression of miR-146a in Alzheimer's disease was 0.97-fold greater than in control, with no statistically significant difference. There were no significant differences in the means between Alzheimer's females and Alzheimer's males. Female control subjects had considerably higher mean ADAS-cog scores than male control subjects. In Alzheimer's disease patients, age was observed to be strongly associated with ADAS-Cog scores, showing that age plays a role in disease development.\u0000 \u0000Conclusion: Serum miR-146a is not related to the development of Alzheimer disease and does not affect disease progression","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42668445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of in vitro antioxidant potential of Anisomeles indica Kuntze and exploration of its bioactive phytoconstituents","authors":"M. T, Kumar Vadlapudi, Prathap H.M., Poornima D.V., R. F., V. E., S. M., Anuradha Cm","doi":"10.51248/.v43i3.2541","DOIUrl":"https://doi.org/10.51248/.v43i3.2541","url":null,"abstract":"Introduction and Aim: Anisomeles indica Kuntze (A. indica) is employed to treat a wide range of illnesses. The Present study was aimed at establishing preliminary phytochemical screening, UV-Visible spectrophotometric, FT-IR analysis, and evaluation of biological activities of A. indica extracts. \u0000  \u0000Materials and Methods: A. indica leaves were employed for the Soxhlet extraction. Furthermore, the crude extracts were utilized for phytochemical analysis and quantitative estimations of phenolics and flavonoids. UV-Vis spectrophotometric, and FT-IR analysis provided further evidence for the existence of bioactive constituents in A. indica extracts. The A. indica extracts were assessed for antioxidant potential by DPPH and metal chelation activity. \u0000  \u0000Results: The findings illustrated that A. indica methanol extract was found to possess the highest yield. The preliminary phytochemical screening, UV-Vis spectrophotometric, and FT-IR fingerprint analysis provided evidence for the existence of significant bioactive constituents. A.indica methanol extract has significant total phenol, flavonoid content, and TAC (total antioxidant capacity) among all extracts. These characteristics are attributed to substantial antioxidant activity and metal-chelating activity. \u0000  \u0000Conclusion: The findings of this study imply that A. indica extract possess antioxidant activity as evaluated by the potential DPPH radical scavenging and chelate metal ions. These characteristics are interconnected to the high flavonoid and phenol content, and distinctive secondary metabolites. The finding indicates that A.indica is abundant in active phytoconstituents, which also offer a vital source for effective therapeutic management.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47313786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}