BioMedicine-Taiwan最新文献

{"title":"Mutation patterns of integrase gene affect antiretroviral resistance in various non-B subtypes of human immunodeficiency virus Type-1 and their implications for patients' therapy.","authors":"Fauzia A Djojosugito, Arfianti Arfianti, Rudi Wisaksana, Agnes R Indrati","doi":"10.37796/2211-8039.1422","DOIUrl":"10.37796/2211-8039.1422","url":null,"abstract":"<p><p>Although primary integrase strand transfer inhibitor resistance mutations are currently uncommon, the increasing use of integrase strand transfer inhibitor as a key component of the first, second and third-line antiretroviral regimens suggests that the prevalence of integrase drug resistance mutations will likely increase. The rise of several polymorphic mutations and natural polymorphisms also affects the level of susceptibility of human immunodeficiency virus (HIV) type-1 to integrase strand transfer inhibitor. The considerable variability among the various subtypes of human immunodeficiency virus type-1 may contribute to differences in integrase mutations associated with integrase strand transfer inhibitors. Notably, non-B subtypes of HIV type-1 (HIV-1) are the predominant cause of human immunodeficiency virus infection worldwide. The presence of diverse integrase drug resistance mutations can have significant implications on the administration of integrase strand transfer inhibitor-based antiretroviral therapy to patients with human immunodeficiency virus infection.</p>","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":"13 4","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10962537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematological biomarkers of troponin, neutrophil-to-lymphocyte ratio, and monocyte-to-lymphocyte ratio serve as effective predictive indicators of high-risk mortality in acute coronary syndrome.","authors":"Bryan G de Liyis, Angela F Ciaves, Marwa H Intizam, Pierre J Jusuf, I Made J Rina Artha","doi":"10.37796/2211-8039.1425","DOIUrl":"10.37796/2211-8039.1425","url":null,"abstract":"<p><strong>Background: </strong>Assessing high-risk mortality in acute coronary syndrome (ACS) patients, encompassing ST-Elevation Myocardial Infarction (STEMI), Non-ST-Elevation Myocardial Infarction (NSTEMI), and Unstable Angina Pectoris (UAP), is crucial. However, the prognostic significance of hematological parameters in predicting high-risk mortality in ACS patients remains uncertain despite advancements in ACS research.</p><p><strong>Aim: </strong>The aim was to investigate prognostic significance of hematological parameters troponin, Creatine Kinase-MB (CKMB), Neutrophil-to-Lymphocyte Ratio (NLR), Platelet-to-Lymphocyte Ratio (PLR), Monocyte-to-Lymphocyte Ratio (MLR), Basophil-to-Lymphocyte Ratio (BLR), and Eosinophil-to-Lymphocyte Ratio (ELR) levels in predicting high-risk mortality in ACS patients.</p><p><strong>Methods: </strong>In this retrospective observational study, data from medical records of 115 patients with ACS, including 40 with STEMI, 38 with NSTEMI, and 37 with UAP, were analyzed. Patients were selected using stratified random sampling, whereby five patients were randomly chosen each month from January 2021 to December 2022 while maintaining a 1:1:1 ratio of selection.</p><p><strong>Results: </strong>Troponin (r = 0.519) and NLR (r = 0.484) showed moderate positive correlations with high-risk STEMI mortality. Meanwhile, troponin (r = 0.387), NLR (r = 0.279), PLR (r = 0.276), MLR (r = 0.250), BLR (r = 0.237), and ELR (r = -0.344) were found to be significantly correlated with high-risk ACS mortality. Troponin, CKMB, NLR, and MLR were significant (AUC>0.7) for high-risk STEMI mortality, and Troponin, NLR, and MLR were significant for high-risk ACS mortality. The results of the multivariate regression analysis indicated that only Troponin (OR:2.049; 95%CI: 1.802-8.218; p = 0.014), NLR (OR:1.652; 95%CI: 1.306-7.753; p = 0.030), and MLR (OR:4.067; 95%CI: 1.182-13.987; p = 0.026) were capable of predicting high-risk ACS mortality. Sub-group analysis showed an increased risk of ACS mortality by GRACE score >140 in patients with elevated levels of Troponin (OR:2.787; 95%CI: 1.032-7.524; p < 0.05), NLR (OR:3.287; 95%CI: 1.340-8.059; p < 0.05), and MLR (OR:4.156; 95%CI: 1.634-10.569; p < 0.05) above the cut-off value.</p><p><strong>Conclusion: </strong>Troponin, NLR, and MLR levels above the cutoff independently predict high-risk mortality in ACS.</p>","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":"13 4","pages":"32-43"},"PeriodicalIF":1.7,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10962536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140295235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of antibacterial efficacy of Lawsonia inermis Linn (henna) on periodontal pathogens using agar well diffusion and broth microdilution methods: an in-vitro study","authors":"Şevki Güler, D. Torul, Sevda Kurt-Bayrakdar, E. K. Tayyarcan, Cagri Camsari, I. Boyaci","doi":"10.37796/2211-8039.1411","DOIUrl":"https://doi.org/10.37796/2211-8039.1411","url":null,"abstract":"Background : Although widely explored in medicine, limited evidence exists in the literature regarding the ef fi cacy of Lawsonia inermis Linn (henna) in the dental fi eld. Aim : This study aimed to investigate the antibacterial effect of henna on Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in vitro. Methods : The agar well diffusion and broth microdilution methods were used to evaluate the antibacterial effect of henna extracts. Dimethyl sulfoxide was used to prepare the ethanol extract of henna, and distilled water was used to prepare the water extract. For both ethanol and water extracts, 4 different concentrations were prepared as 15, 30, 60, and 120 mg/mL. Results : It was determined that the water and ethanol extracts of the henna samples did not show an inhibition zone on P.gingivalis and A.actinomycetemcomitans. As a result of the evaluations made with the broth microdilution method, it was found that the ethanol extract had a higher inhibitory effect on both bacteria, and both extracts had more inhibitory effects against A.actinomycetemcomitans. Conclusion : To understand the effect of henna on periodontal pathogens, more comprehensive in vitro studies should be performed on henna samples at different concentrations and with different bases.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42342193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hipoxia modulates the secretion of growth factors in of human umbilical cord-derived mesenchymal stem cells","authors":"Arfianti Arfianti, U. Ulfah, Leopold Sampetua Hutabarat, Agnes Ivana G, A. Budiarti, N. Sahara, Nicko Pisceski Kusika Saputra","doi":"10.37796/2211-8039.1416","DOIUrl":"https://doi.org/10.37796/2211-8039.1416","url":null,"abstract":"Background : Mesenchymal stem cell (MSC) has great potential as therapies due its ability to regenerate tissue damage and promote tissue homeostasis. Preconditioning of MSC in low oxygen concentration has been shown to affect the therapeutic potential of these cells. This study aimed to compare the characteristic and secretion of trophic factors of MSCs cultured under hypoxia and normoxia. Methods : MSCs were isolated from Wharton ' s jelly of human umbilical cord (UC) tissue by explant method and characterized by fl ow cytometry. Following 24 h of CoCl 2 -induced hypoxic culture, the viability and metabolic activity of MSC were analyzed by trypan blue exclusion test and methyl thiazolyl tetrazolium (MTT) assay, respectively. The secretion of hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) was assessed in conditioned medium using enzyme-linked immunosorbent assay (ELISA) method. Results : Flow cytometry analysis showed > 99% of the population of MSCs cells were positive for CD73 and CD90 and > 62% were positive for CD105. While the cell viability of MSC was not affected by hypoxic cultured condition, the metabolic activity rate of these cells was decreased under hypoxic conditioning. In line with reduced metabolic activity, hypoxic human UC-derived MSC produced less HGF than normoxic counterpart. Compared to normoxic MSC, hypoxic preconditioned MSC secreted higher level of VEGF in the conditioned medium (p < 0.05). Conclusions : Hypoxia decreased the metabolic activity of MSCs associated with the modulation of HGF and VEGF secretions. It is suggested that hypoxia may also affect the therapeutic capacity of MSC cells.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45122123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid profile in patients with leiomyoma-A case-control study","authors":"Kishan Prasad H.L., Nimmy Maria Dasan, A. Kishan, S. Kumari N","doi":"10.51248/.v43i3.1443","DOIUrl":"https://doi.org/10.51248/.v43i3.1443","url":null,"abstract":"Introduction and Aim:  The most frequent gynaecological neoplasm in women of reproductive age is leiomyoma (Fibroid). They are hormone-dependent tumours, and estrogen will promote their development. It has been found that estrogen levels and cholesterol levels are inversely associated.  Few studies have shown no relation between lipid profile and leiomyoma, but few studies suggested a positive correlation. The lipid profile of patients with uterine fibroids (the case group) and women without fibroids (the control group) is compared in the current study.\u0000 \u0000Materials and Methods: The case-control study included fifty subjects between 20 to 40 years. Twenty-five serum samples collected from women without evidence of leiomyoma were considered the control group. Twenty-five cases were admitted with various symptoms, diagnosed and confirmed as leiomyoma on histopathology.\u0000 \u0000Results: There was a substantial difference in Total Cholesterol (TC) and Low-Density Lipoprotein cholesterol (LDL-C) levels between patients with leiomyoma and healthy people.  With a p-value of 0.04, the TC, LDL-C level in leiomyoma patients was substantially lower than that of healthy individuals in the general population. The levels of triglycerides, High-Density Lipoprotein cholesterol (HDL-C), and Very Low-Density Lipoprotein cholesterol (VLDL-C) were comparable across the case and control groups, though.\u0000 \u0000Conclusion: According to the study's findings, patients with leiomyoma have lower serum TC and LDL-C levels than healthy controls. This could be linked to patients with leiomyomas having higher estrogen levels. As a result, lipid profile variables like TC and LDL-C may be accurate indicators of leiomyoma. These findings point to the possibility of blood plasma lipid profiling for the non-invasive diagnosis of fibroids.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47560798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypervitaminosis D induced hypercalcemia leading to coma: A case report","authors":"Bimal K. Agrawal, Manu Mathew, S. Kalia, Aditi Tongar","doi":"10.51248/.v43i3.3192","DOIUrl":"https://doi.org/10.51248/.v43i3.3192","url":null,"abstract":"An elderly female presented with abdominal pain, vomiting and easy fatigability. Her sensorium gradually declined and became comatose. She was on Methotrexate, folic acid and prednisolone for her rheumatoid arthritis. The neurological examination and neuroimaging did not contribute in making the diagnosis. It is challenging to diagnose a metabolic cause of coma. Once diagnosis is established, the management becomes easier. On detailed investigation, she was found to have severe hypercalcemia with corrected serum calcium level being 18.2 mg%. Serum vitamin D level was also high, 150 ng/ml. On probing for detailed history, it was revealed that she had been taking a weekly dose of 60,000 IU of vitamin D for nearly 5 years. In recent times, association of various diseases has been reported with vitamin D deficiency. Vitamin D supplementation seems justified in this patient considering her age and the fact that she had rheumatoid arthritis; she was also taking corticosteroid. The recommended dose of vitamin D has been 400–800 IU/day. However, it is often prescribed at a dose of 60,000 IU/week, and sometimes patients self-medicate. There are no guidelines available for prescribing vitamin D at such a strength. Though safety of vitamin D has been established, vitamin D toxicity can occur sporadically with serious consequences. This was managed with intravenous fluids and diuretics. The patient also required subcutaneous calcitonin and low dose of corticosteroid, in addition to a session of haemodialysis for her hypercalcemia. The patient’s sensorium gradually improved as her calcium level returned to normalcy.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":"1 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41320009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the cognitive status of Covid -19 patients and its correlation to blood pressure","authors":"Mamatha S.D., Priya S.A., S. L., Shivakumar K.M., Smitha Malenahalli Chandrashekarappa","doi":"10.51248/.v43i3.2264","DOIUrl":"https://doi.org/10.51248/.v43i3.2264","url":null,"abstract":"Introduction and Aim: The disease caused by Corona virus (Covid-19) has become a public health importance in recent times as its human-to-human transmission is faster when compared to other pandemic of recent times. Since the outbreak of Covid-19, epidemiology and clinical characteristics of the disease including spread of the disease and mortality have been reported extensively. Little is known about Covid 19 impact on cognition; hence the present was undertaken. The study aimed at assessing the cognitive status in patients admitted for infection with corona and to determine the correlation between cognitive status and blood pressure in the same patients. \u0000  \u0000Materials and Methods: The study is a cross-sectional study that included 300 adults of both genders admitted in the tertiary care hospital with RTPCR positive for Covid-19. A cognition failure questionnaire was used to record the patient’s response and also BP was recorded. \u0000      \u0000Results: For analysis Descriptive statistics like mean and Standard Deviation, and to find association between parameters - Spearman correlation, Mann Whitney U Test were used. Analysis was done using SPSS version 22. Even though there was no significant association seen between Overall cognitive scores, forgetfulness scores, and false triggering scores with gender, there was significant association seen between overall cognitive scores and false triggering scores with age. Applying spearman correlation with respect to overall cognitive failure with duration of hospital stay showed statistically significance*(p<0.001) and between cognitive failure and diastolic blood pressure showed statistical significance with respect to overall cognition. \u0000  \u0000Conclusion: Infection with Covid-19 has an effect on cognition. Duration of hospital stay has an impact on cognition with associated blood pressure changes in the Covid-19 disease.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46557930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glandular odontogenic cyst coexisting with actinomycosis: A rare case report","authors":"Priyanka Madhavan, Shruti D Nayak, S. Prabhu, M. Jose","doi":"10.51248/.v43i3.2325","DOIUrl":"https://doi.org/10.51248/.v43i3.2325","url":null,"abstract":"Jaw lesions such as radicular cyst, odontogenic keratocyst, osteomyelitis associated with Actinomyces have been reported by many authors in the literature. Actinomycotic colonization in developmental cysts like GOC is extremely rare. So far in the literature search, the previous reports have not described actinomyces co-existing with GOC to our knowledge. Herein, we report a rare case report of GOC coexisting with actinomycotic colonies and pathogenesis of actinomycosis associated with odontogenic cyst.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48542570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sinonasal lesions – Case analysis at a tertiary care centre in coastal India","authors":"M. Khadilkar, Deviprasad Dosemane, U. Khadilkar","doi":"10.51248/.v43i3.2591","DOIUrl":"https://doi.org/10.51248/.v43i3.2591","url":null,"abstract":"Introduction and Aim: The sinonasal region is affected by different types of neoplastic and non-neoplastic conditions. This study attempts to highlight our experience with these lesions and to categorize them.\u0000 \u0000Methods: A retrospective study was conducted in our hospitals and data including history, clinical findings, and histopathological diagnosis was collected from medical records of 187 patients who visited the outpatient department with nasal, paranasal sinus or nasopharyngeal lesions from 2007 to 2020.\u0000 \u0000Results: A total of 187 patients were included. The commonest site involved was the nasal cavity in 104 (55.61%) patients, followed by maxillary sinus in 26 (13.9%) patients and nasopharynx in 16 (8.56%) patients. The majority of the cases were benign tumours (41.71%), followed by malignancy (35.29%). Inverted papilloma was the commonest benign tumour (13.9%); squamous cell carcinoma was the most frequent type of malignancy (12.3%).\u0000 \u0000Conclusion: The overlapping features of nasal masses make diagnosis a challenge, the role of histopathology is indispensable. Early diagnosis and treatment are essential for better clinical management, which will ultimately enhance healthcare.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47237147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological evaluation for haematinic activity of Siddha formulation Lavana Dravagam in rat model","authors":"Jeeva S., Kesavarajan S., M. A, S. S, Meenakaumari R., R. Sudalaimani","doi":"10.51248/.v43i3.2135","DOIUrl":"https://doi.org/10.51248/.v43i3.2135","url":null,"abstract":"Introduction and Aim:  Anaemia is the most common deficiency disorder among people of all age groups, known as Paandu in the Siddha system of medicine. The test drug siddha poly mineral formulation Lavana Dravagam mentioned in Siddha literature kannukamiyam ennum vaithiya segaram, has been used for pandu (anaemia). Dravagam is a form of internal medicine, processed by distillation method and is also referred as Pugai neer, Shakthi neer, Dravaga neer. The study aimed to evaluate the haematinic activity and efficacy of poly mineral Siddha formulation Lavana Dravagam against phenylhydrazine induced anaemic albino Wistar rat model.\u0000 \u0000Materials and Methods:  The animals were selected and divided into four groups (I, II, III, and IV) of six rats (n=6) each. Anaemia was induced by an oral administration of phenylhydrazine (single dose of 10 mg/kg per oral for 8 days). Group I served as normal control and Group II received standard drug hematinic syrup in suspension form at dose 2 mL/kg. Groups III, IV received the formulated oral indiffusible mixture of Lavana Dravagam at a dose of 0.02ml to 0.03ml/kg respectively. RBC, Hb, PCV, MCV, MCH, were analyzed as indices of anaemia. The mean corpuscular volume, mean corpuscular Hb, and mean corpuscular Hb concentration were calculated.\u0000 \u0000Results: From the literature evidence, acute toxicity evaluation and pharmacological studies, the drug Lavana Dravagam is found out to have hematinic activity. This study reveals that there is significant (P<0.05) increase in RBC count, Hb level, and PCV by administering phenylhydrazine within one week of treatment.\u0000 \u0000Conclusion: It could be concluded that the drug Lavana dravagam will have promising effects in the management of anaemia (Paandu).","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42181196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}