Alfonso Mata-Bermudez, Ricardo Trejo-Chávez, Marina Martínez-Vargas, Adán Pérez-Arredondo, María de Los Ángeles Martínez-Cardenas, Araceli Diaz-Ruiz, Camilo Rios, Héctor A Romero-Sánchez, Agustino Martínez-Antonio, Luz Navarro
{"title":"牡丹籽提取物对轻度脑外伤大鼠抑郁样行为的影响","authors":"Alfonso Mata-Bermudez, Ricardo Trejo-Chávez, Marina Martínez-Vargas, Adán Pérez-Arredondo, María de Los Ángeles Martínez-Cardenas, Araceli Diaz-Ruiz, Camilo Rios, Héctor A Romero-Sánchez, Agustino Martínez-Antonio, Luz Navarro","doi":"10.37796/2211-8039.1461","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Traumatic brain injury (TBI) is a severe health problem for which there is no specific treatment, leading to neurological or neuropsychological consequences. One of the most described disorders, even after mild TBI (mTBI), is depression, related to mechanisms involving reactive oxygen species (ROS). The <i>Mucuna pruriens</i> (<i>M. pruriens</i>) plant has various antioxidant, neuroprotective, and anti-inflammatory properties.</p><p><strong>Purpose: </strong>There is insufficient evidence of <i>M. pruriens</i> use for the treatment of neurobehavioral and depressive impairments induced by TBI and of the mechanisms underlying this effect, so we aimed to evaluate the ability of shortterm administration of <i>M. pruriens</i> extract to prevent neurobehavioral impairment and depression-like behaviors in a murine model of mTBI as well as evaluate the role of oxidative stress.</p><p><strong>Methods: </strong>Male Wistar rats underwent mTBI or sham surgery. Immediately after, they were treated with vehicle or <i>M. pruriens</i> extract (50 mg/kg ip/day for five days). We evaluated neurobehavioral recovery using the Neurobehavioral Severity Scale-Revised (NSS-R) and the immobility time in the forced swimming test 3, 7, 15, 30, and 60 days after mTBI. In addition, lipid peroxidation (LP) and GSH concentrations were determined in some brain areas (motor cortex, striatum, midbrain, and nucleus accumbens).</p><p><strong>Results: </strong><i>M. pruriens</i> extract did not decrease neurobehavioral impairment caused by mTBI. Nevertheless, it prevented depression-like behaviors starting three days after mTBI, reduced LP, and increased GSH in some brain areas. <i>Conclusions</i>: <i>M. pruriens</i> may prevent depression-like behaviors and reduce oxidative stress by decreasing LP and increasing concentrations of antioxidant compounds.</p>","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":"14 3","pages":"23-30"},"PeriodicalIF":2.1000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460573/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effect of <i>Mucuna pruriens</i> seed extract on depression-like behavior derived from mild traumatic brain injury in rats.\",\"authors\":\"Alfonso Mata-Bermudez, Ricardo Trejo-Chávez, Marina Martínez-Vargas, Adán Pérez-Arredondo, María de Los Ángeles Martínez-Cardenas, Araceli Diaz-Ruiz, Camilo Rios, Héctor A Romero-Sánchez, Agustino Martínez-Antonio, Luz Navarro\",\"doi\":\"10.37796/2211-8039.1461\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Traumatic brain injury (TBI) is a severe health problem for which there is no specific treatment, leading to neurological or neuropsychological consequences. One of the most described disorders, even after mild TBI (mTBI), is depression, related to mechanisms involving reactive oxygen species (ROS). The <i>Mucuna pruriens</i> (<i>M. pruriens</i>) plant has various antioxidant, neuroprotective, and anti-inflammatory properties.</p><p><strong>Purpose: </strong>There is insufficient evidence of <i>M. pruriens</i> use for the treatment of neurobehavioral and depressive impairments induced by TBI and of the mechanisms underlying this effect, so we aimed to evaluate the ability of shortterm administration of <i>M. pruriens</i> extract to prevent neurobehavioral impairment and depression-like behaviors in a murine model of mTBI as well as evaluate the role of oxidative stress.</p><p><strong>Methods: </strong>Male Wistar rats underwent mTBI or sham surgery. Immediately after, they were treated with vehicle or <i>M. pruriens</i> extract (50 mg/kg ip/day for five days). We evaluated neurobehavioral recovery using the Neurobehavioral Severity Scale-Revised (NSS-R) and the immobility time in the forced swimming test 3, 7, 15, 30, and 60 days after mTBI. In addition, lipid peroxidation (LP) and GSH concentrations were determined in some brain areas (motor cortex, striatum, midbrain, and nucleus accumbens).</p><p><strong>Results: </strong><i>M. pruriens</i> extract did not decrease neurobehavioral impairment caused by mTBI. 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引用次数: 0
摘要
背景:创伤性脑损伤(TBI)是一种严重的健康问题,目前尚无特效治疗方法,会导致神经或神经心理方面的后果。即使在轻度创伤性脑损伤(mTBI)后,抑郁症也是描述最多的疾病之一,这与涉及活性氧(ROS)的机制有关。目的:目前还没有足够的证据表明 M. pruriens 可用于治疗创伤性脑损伤引起的神经行为障碍和抑郁障碍,也没有足够的证据表明这种作用的机制,因此我们旨在评估短期服用 M. pruriens 提取物预防小鼠创伤性脑损伤模型中神经行为障碍和抑郁样行为的能力,以及评估氧化应激的作用:方法:雄性 Wistar 大鼠接受 mTBI 或假手术。方法:雄性 Wistar 大鼠接受了 mTBI 或假手术,术后立即接受药物或 M. pruriens 提取物(50 毫克/千克 ip/天,连续五天)治疗。我们使用神经行为严重程度量表-修订版(NSS-R)和强迫游泳测试中的不动时间来评估大鼠神经行为的恢复情况,时间分别为创伤性脑损伤后3、7、15、30和60天。此外,还测定了一些脑区(运动皮层、纹状体、中脑和伏隔核)的脂质过氧化(LP)和 GSH 浓度:结果:M. pruriens 提取物并没有减轻 mTBI 引起的神经行为损伤。然而,它能预防创伤后三天开始出现的抑郁样行为,降低LP,并增加某些脑区的GSH。结论M.pruriens可通过降低LP和增加抗氧化化合物的浓度来预防类似抑郁症的行为并减少氧化应激。
Effect of Mucuna pruriens seed extract on depression-like behavior derived from mild traumatic brain injury in rats.
Background: Traumatic brain injury (TBI) is a severe health problem for which there is no specific treatment, leading to neurological or neuropsychological consequences. One of the most described disorders, even after mild TBI (mTBI), is depression, related to mechanisms involving reactive oxygen species (ROS). The Mucuna pruriens (M. pruriens) plant has various antioxidant, neuroprotective, and anti-inflammatory properties.
Purpose: There is insufficient evidence of M. pruriens use for the treatment of neurobehavioral and depressive impairments induced by TBI and of the mechanisms underlying this effect, so we aimed to evaluate the ability of shortterm administration of M. pruriens extract to prevent neurobehavioral impairment and depression-like behaviors in a murine model of mTBI as well as evaluate the role of oxidative stress.
Methods: Male Wistar rats underwent mTBI or sham surgery. Immediately after, they were treated with vehicle or M. pruriens extract (50 mg/kg ip/day for five days). We evaluated neurobehavioral recovery using the Neurobehavioral Severity Scale-Revised (NSS-R) and the immobility time in the forced swimming test 3, 7, 15, 30, and 60 days after mTBI. In addition, lipid peroxidation (LP) and GSH concentrations were determined in some brain areas (motor cortex, striatum, midbrain, and nucleus accumbens).
Results: M. pruriens extract did not decrease neurobehavioral impairment caused by mTBI. Nevertheless, it prevented depression-like behaviors starting three days after mTBI, reduced LP, and increased GSH in some brain areas. Conclusions: M. pruriens may prevent depression-like behaviors and reduce oxidative stress by decreasing LP and increasing concentrations of antioxidant compounds.