Reproduction, fertility, and development最新文献_第8页

Clustering of spermatozoa examined through flow cytometry provides more information than the conventional assessment: a resilience to osmotic stress example. 通过流式细胞仪对精子进行聚类检测，可提供比传统评估更多的信息：以抗渗透压能力为例。

Reproduction, fertility, and development Pub Date : 2024-05-01 DOI: 10.1071/RD23132

Julian Valencia, Sebastián Bonilla-Correal, Elisabeth Pinart, Sergi Bonet, Marc Yeste

{"title":"Clustering of spermatozoa examined through flow cytometry provides more information than the conventional assessment: a resilience to osmotic stress example.","authors":"Julian Valencia, Sebastián Bonilla-Correal, Elisabeth Pinart, Sergi Bonet, Marc Yeste","doi":"10.1071/RD23132","DOIUrl":"https://doi.org/10.1071/RD23132","url":null,"abstract":"Context Conventional sperm quality tests may not be sufficient to predict the fertilising ability of a given ejaculate; thus, rapid, reliable and sensitive tests are necessary to measure sperm function. Aims This study sought to address whether a cluster analysis approach based on flow cytometry variables could provide more information about sperm function. Methods Spermatozoa were exposed to either isotonic (300mOsm/kg) or hypotonic (180mOsm/kg) media for 5 and 20min, and were then stained with SYBR14 and propidium iodide (PI). Based on flow cytometry dot plots, spermatozoa were classified as either viable (SYBR14+ /PI- ) or with different degrees of plasma membrane alteration (SYBR14+ /PI+ and SYBR14- /PI+ ). Moreover, individual values of electronic volume (EV), side scattering (SS), green (FL1) and red (FL3) fluorescence were recorded and used to classify sperm cells through cluster analysis. Two strategies of this approach were run. The first one was based on EV and the FL3/FL1 quotient, and the second was based on EV, SS and the FL3/FL1 quotient. Key results The two strategies led to the identification of more than three sperm populations. In the first strategy, EV did not differ between membrane-intact and membrane-damaged sperm, but it was significantly (P P P Conclusions Cluster analysis based on flow cytometry variables provides more information about sperm function than conventional assessment does. Implications Combining flow cytometry with cluster analysis is a more robust approach for sperm evaluation.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"36 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141072400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of bull age, sperm processing, and microclimatic conditions on the viability and DNA integrity of cryopreserved bovine sperm. 公牛年龄、精子处理和微气候条件对冷冻保存牛精子的存活率和 DNA 完整性的影响。

Reproduction, fertility, and development Pub Date : 2024-05-01 DOI: 10.1071/RD23219

Burcu Cinar, Heinrich Bollwein, Mathias Siuda, Matthias Lautner, Claus Leiding, Eleni Malama

{"title":"Impact of bull age, sperm processing, and microclimatic conditions on the viability and DNA integrity of cryopreserved bovine sperm.","authors":"Burcu Cinar, Heinrich Bollwein, Mathias Siuda, Matthias Lautner, Claus Leiding, Eleni Malama","doi":"10.1071/RD23219","DOIUrl":"https://doi.org/10.1071/RD23219","url":null,"abstract":"Context Seasonal microclimatic fluctuations can cause changes in sperm quality even in dairy bulls bred under temperate climate. These changes can vary between sires of different age and affect sperm freezability. Aims We aimed to evaluate the modulating effect of bull age and equilibration time before freezing on the seasonal pattern of sperm viability and DNA integrity post-thaw. Methods In the frame of systematic sperm quality control, we assessed the integrity of sperm plasma membrane and acrosome (PMAI) in 15,496 cryopreserved bovine batches, and the percentage of sperm with high DNA fragmentation index (%DFI) after 0h and 3h incubation at 38°C post-thaw (3h) in 3422 batches. Semen was equilibrated for 24h before freezing if collected on Monday or Wednesday and 72h if produced on Friday. We investigated the effect of season, bull age, equilibration, and temperature-humidity index (THI) on the day of semen collection on sperm traits using mixed-effects linear models. Key results PMAI and %DFI (0h and 3h) deteriorated with increasing THI. The effect of THI on %DFI was detected with a 30-day time lag. Seasonal fluctuations of sperm quality were similar between young, mature, and older sires. Prolonged equilibration did not affect PMAI but was linked to elevated %DFI (3h) in summer. Conclusions Extending equilibration from 24 to 72h is compatible with commercial standards of bovine sperm quality post-thaw; however, it could interfere with the seasonal pattern of the latter. Implications Systematic monitoring of bovine sperm quality enables the prompt detection of stress factors related to microclimate and semen processing.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"36 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140878380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotyping of rams based on melatonin receptor 1A gene polymorphisms: a tool in sire selection? 基于褪黑激素受体 1A 基因多态性的公羊基因分型：选择母羊的工具？

Reproduction, fertility, and development Pub Date : 2024-05-01 DOI: 10.1071/RD23235

Victoria Peña-Delgado, Agustí Noya, Melissa Carvajal-Serna, Francisco Canto, María Carmen Sánchez, Eva Letosa, Antonio Vicente, Ignacio Morato, Ángel Macías, José Alfonso Abecia, Adriana Casao, Rosaura Pérez-Pe

{"title":"Genotyping of rams based on melatonin receptor 1A gene polymorphisms: a tool in sire selection?","authors":"Victoria Peña-Delgado, Agustí Noya, Melissa Carvajal-Serna, Francisco Canto, María Carmen Sánchez, Eva Letosa, Antonio Vicente, Ignacio Morato, Ángel Macías, José Alfonso Abecia, Adriana Casao, Rosaura Pérez-Pe","doi":"10.1071/RD23235","DOIUrl":"10.1071/RD23235","url":null,"abstract":"Context Several polymorphisms in the melatonin receptor 1A gene (MTNR1A ) have been related to reproductive performance in ovine. Aims To investigate the effect of the Rsa I and Mnl I polymorphisms on ram seminal quality. Methods Eighteen Rasa Aragonesa rams were genotyped for the Rsa I (C/C, C/T, T/T) and Mnl I (G/G, G/A, A/A) allelic variants of the MTNR1A gene. Individual ejaculates were analysed once a month throughout the whole year. Sperm motility, morphology, membrane integrity, levels of reactive oxygen species (ROS), phosphatidylserine (PS) inversion, DNA fragmentation and capacitation status were assessed. The effect of the season and polymorphisms on seminal quality was evaluated by mixed ANOVA. Key results Both polymorphisms had an effect on membrane integrity and viable spermatozoa with low levels of ROS and without PS translocation, and Rsa I also on motile and DNA-intact spermatozoa. An interaction between both polymorphisms was found, pointing to a negative effect on seminal quality of carrying the T or A allele in homozygosity. Differences were higher in the reproductive than in the non-reproductive season. Conclusions Mutations substituting C by T and G by A at Rsa I and Mnl I polymorphic sites, respectively, in the MTNR1A gene in rams could decrease the seminal quality. Implications Genotyping of rams based on melatonin receptor 1A could be a powerful tool in sire selection.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"36 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140961450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The spatio-temporal distribution of aromatase cytochrome in ovary throughout the canine oestrous cycle. 犬整个发情周期卵巢中芳香化酶细胞色素的时空分布。

Reproduction, fertility, and development Pub Date : 2024-03-01 DOI: 10.1071/RD23201

L Lindh, M P Kowalewski, S K Goericke-Pesch, H Lindeberg, G Schuler, O A T Peltoniemi

{"title":"The spatio-temporal distribution of aromatase cytochrome in ovary throughout the canine oestrous cycle.","authors":"L Lindh, M P Kowalewski, S K Goericke-Pesch, H Lindeberg, G Schuler, O A T Peltoniemi","doi":"10.1071/RD23201","DOIUrl":"10.1071/RD23201","url":null,"abstract":"Context: New animal welfare legislation and ethical guidelines encourage alternative approaches for canine contraception, instead of surgical gonadectomy which is considered invasive and unjustified in healthy dogs.Aims: Reversible contraception might be achieved by inhibition of aromatase (CYP19), an enzyme catalysing the conversion of androgens to oestrogens. This study provides insights into the spatio-temporal expression and distribution of aromatase in canine ovarian tissue.Methods: Ovarian tissue was collected from 39 healthy and sexually mature bitches during different stages of the oestrous cycle: pro-oestrus (n =8), oestrus (n =12), dioestrus (n =9) (luteal phase) and anoestrus (n =10). Localisation of cytochrome P450 aromatase was determined by immunohistochemistry.Key results: Aromatase activity in the dog is high during pro-oestrus, ovulation and early dioestrus. Comparing types of follicles and corpora lutea, the highest aromatase abundance was found in antral follicles and luteinising follicles, whereas corpora lutea and early antral follicles showed an intermediate presence of the enzyme. Interesting was the high abundance of aromatase in luteinising theca interna cells, prevailing over granulosa cells.Conclusions and implications: Understanding of cells involved in oestradiol production is important for targeted inhibition of oestradiol synthesis, possibly offering an approach for contraception and suppression of oestrus.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140133740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotics in semen extenders - a multiplicity of paradoxes. 精液增稠剂中的抗生素--多重悖论。

Reproduction, fertility, and development Pub Date : 2024-03-01 DOI: 10.1071/RD23218

Jane M Morrell, Aleksandar Cojkic, Pongpreecha Malaluang, Theodoros Ntallaris, Johanna Lindahl, Ingrid Hansson

引用次数: 0

The future of equine semen analysis. 马精液分析的未来。

Reproduction, fertility, and development Pub Date : 2024-03-01 DOI: 10.1071/RD23212

Fernando J Peña, Francisco Eduardo Martín-Cano, Laura Becerro-Rey, Cristina Ortega-Ferrusola, Gemma Gaitskell-Phillips, Eva da Silva-Álvarez, María Cruz Gil

引用次数: 0

The semen microbiome of miniature pony stallions. 迷你矮种马的精液微生物群。

Reproduction, fertility, and development Pub Date : 2024-02-01 DOI: 10.1071/RD23117

C Giselle Cooke, Zamira Gibb, Christopher G Grupen, Kathrin Schemann, Nandan Deshpande, Joanna E Harnett

{"title":"The semen microbiome of miniature pony stallions.","authors":"C Giselle Cooke, Zamira Gibb, Christopher G Grupen, Kathrin Schemann, Nandan Deshpande, Joanna E Harnett","doi":"10.1071/RD23117","DOIUrl":"10.1071/RD23117","url":null,"abstract":"Context: Little is known about the microbial composition of stallion semen.Aims: To describe the microbiota detected in equine semen of healthy miniature pony stallions.Methods: Semen specimens were collected using a Missouri artificial vagina at a single time point. PacBio (Pacific Biosciences) genomic DNA sequencing of the 16S rRNA gene was performed on these specimens, following which next-generation microbiome bioinformatics platform QIIME2 was used to process fastq files and analyse the amplicon data. The data were categorised into genus, family, class, order and phylum.Key results: Firmicutes and Bacteroidetes phyla predominated (76%), followed by Proteobacteria (15%). Bacteroidales, Clostridiales and Cardiobacteriales predominated the microbial rank of order (86%). Class was mainly composed of Bacteroidia, Clostridia and Gammaproteobacteria (87%), while family was mainly composed of Porphyromonadaceae , Family_XI and Cardiobacteriaceae (62%). At the level of genus, 80% of the abundance was composed of seven genera, namely Porphyromonas, Suttonella, Peptoniphilus, Fastidiosipila, Ezakiella, Petrimonas and an unknown taxon.Conclusions: The findings indicate that specific microbiota may be characteristic of healthy miniature pony stallions' semen with some inter-individual variations observed.Implications: Larger equine studies involving fertile and infertile subjects could be informed by this study and could explore the relationship of the semen microbiome to male fertility.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139708947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lycopene inhibits apoptosis of mouse spermatocytes in varicocele via miR-23a/b-induced downregulation of PROK2. 番茄红素通过 miR-23a/b 诱导的 PROK2 下调抑制精索静脉曲张中小鼠精母细胞的凋亡。

Reproduction, fertility, and development Pub Date : 2024-02-01 DOI: 10.1071/RD23136

Hongqiang Wang, Baojuan Zhu, Tao Jing, Lei Yu, Kaishu Zhang, Yujie Liu, Hanshu Wang

{"title":"Lycopene inhibits apoptosis of mouse spermatocytes in varicocele via miR-23a/b-induced downregulation of PROK2.","authors":"Hongqiang Wang, Baojuan Zhu, Tao Jing, Lei Yu, Kaishu Zhang, Yujie Liu, Hanshu Wang","doi":"10.1071/RD23136","DOIUrl":"10.1071/RD23136","url":null,"abstract":"Context The varicocele is the leading cause of male infertility and can impair sperm quality and testicular function through various mechanisms. In our previous study, we found that lycopene could attenuate hypoxia-induced testicular injury. Aims To illustrate the detailed mechanism of lycopene on spermatocytes. Methods The effect of lycopene on GC-2 cells under hypoxia were detected by flow cytometry and western blot assay. miR-seq was used to determine miRNA expression in varicocele rat model testes. The function of miR-23a/b were determined by flow cytometry and western blot assay. Key results We demonstrate that lycopene could alleviate hypoxia-induced GC-2 cell apoptosis and could elevate miR-23a/b expression of the hypoxia model in vivo and in vitro . The miR-23a and -23b mimics could reduce the hypoxia-induced GC-2 cell apoptosis. Both miR-23a and -23b could directly bind with prokineticin 2 (PROK2) mRNA and downregulate its expression. Conclusions Lycopene could attenuate hypoxia-induced spermatocyte injury through the miR-23a/b-PROK2 pathway. Implications Lycopene may be an effective treatment for varicocele to improve testicular impairment.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139674026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The case for considering the term 'mitochondrial vesicle' as a misnomer in publications about assisted reproductive technologies (ART) for amphibians. 在有关两栖动物辅助生殖技术（ART）的出版物中，将 "线粒体囊泡 "一词视为误称的理由。

Reproduction, fertility, and development Pub Date : 2024-02-01 DOI: 10.1071/RD24014

Robert K Browne, Govindappa Venu, Svetlana A Kaurova

引用次数: 0

Recent advances in reproductive research in Australia and New Zealand: highlights from the Annual Meeting of the Society for Reproductive Biology, 2022. 澳大利亚和新西兰生殖研究的最新进展：2022 年生殖生物学学会年会要点。

Reproduction, fertility, and development Pub Date : 2024-02-01 DOI: 10.1071/RD23213

Ella S Green, Hon Y Chan, Emily Frost, Meaghan Griffiths, Jennifer Hutchison, Jacinta H Martin, Bettina P Mihalas, Trent Newman, Jessica E M Dunleavy

{"title":"Recent advances in reproductive research in Australia and New Zealand: highlights from the Annual Meeting of the Society for Reproductive Biology, 2022.","authors":"Ella S Green, Hon Y Chan, Emily Frost, Meaghan Griffiths, Jennifer Hutchison, Jacinta H Martin, Bettina P Mihalas, Trent Newman, Jessica E M Dunleavy","doi":"10.1071/RD23213","DOIUrl":"10.1071/RD23213","url":null,"abstract":"In 2022, the Society for Reproductive Biology came together in Christchurch New Zealand (NZ), for its first face-to-face meeting since the global COVID-19 pandemic. The meeting showcased recent advancements in reproductive research across a diverse range of themes relevant to human health and fertility, exotic species conservation, and agricultural breeding practices. Here, we highlight the key advances presented across the main themes of the meeting, including advances in addressing opportunities and challenges in reproductive health related to First Nations people in Australia and NZ; increasing conservation success of exotic species, including ethical management of invasive species; improvements in our understanding of developmental biology, specifically seminal fluid signalling, ovarian development and effects of environmental impacts such as endocrine-disrupting chemicals; and leveraging scientific breakthroughs in reproductive engineering to drive solutions for fertility, including in assisted reproductive technologies in humans and agricultural industries, and for regenerative medicine.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139725637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0