Reproduction, fertility, and development最新文献

{"title":"The role of the oviduct environment in embryo survival.","authors":"Jennifer L Juengel, Karen L Reader, Paul H Maclean, Laurel D Quirke, Sylvia Zellhuber-McMillan, Neville A Haack, Axel Heiser","doi":"10.1071/RD23171","DOIUrl":"10.1071/RD23171","url":null,"abstract":"<p><strong>Context: </strong>Declining fertility is an issue in multiple mammalian species. As the site of fertilisation and early embryo development, the oviduct plays a critical role in embryo survival, yet there is a paucity of information on how the oviduct regulates this process.</p><p><strong>Aims: </strong>We hypothesised that differences in steroid hormone signalling and/or immune function would be observed in a model of poor embryo survival, the peripubertal ewe.</p><p><strong>Methods: </strong>We examined expression of steroid hormones in systemic circulation, oviductal expression of oestrogen receptorαand genes important in steroid hormone signalling, and immune function in pregnant and cyclic peripubertal and adult ewes on day 3 after oestrus.</p><p><strong>Key results: </strong>Concentrations of progesterone, but not oestradiol, were decreased in the peripubertal ewe compared to the adult ewe. Oestrogen receptorαprotein expression was increased in the peripubertal ewe, but pathway analysis of gene expression revealed downregulation of the oestrogen signalling pathway compared to the adult ewe. Differential expression of several genes involved in immune function between the peripubertal and adult ewe was consistent with an unfavourable oviductal environment in the peripubertal ewe lamb. Oestradiol concentration was positively correlated with the expression of multiple genes involved in the regulation of immune function.</p><p><strong>Conclusions: </strong>Differences in the immune environment of the oviduct, potentially linked to differential modulation by steroid hormones, may partially underly the poor fertilisation and early embryo survival observed in the peripubertal ewe.</p><p><strong>Implications: </strong>A unfavourable oviductal environment may play an important role in limiting reproductive success.</p>","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139975357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alterations of the gut microbiota and fecal short-chain fatty acids in women undergoing assisted reproduction.","authors":"Ni Wu, Jun Liu, Yu Sun, Xiaoxiao Fan, Tianzi Zang, Brianna N Richardson, Jinbing Bai, Yunyan Xianyu, Yanqun Liu","doi":"10.1071/RD23096","DOIUrl":"10.1071/RD23096","url":null,"abstract":"<p><strong>Context: </strong>The community structure of gut microbiota changes during pregnancy, which also affects the synthesis of short-chain fatty acids (SCFAs). However, the distribution of gut microbiota composition and metabolite SCFA levels are poorly understood in women undergoing assisted reproductive technology (ART).</p><p><strong>Aims: </strong>To evaluate the changes in gut microbiota composition and metabolic SCFAs in women who received assisted reproduction treatment.</p><p><strong>Methods: </strong>Sixty-three pregnant women with spontaneous pregnancy (SP) and nine with ART pregnancy were recruited to provide fecal samples. Gut microbiota abundance and SCFA levels were determined by 16S ribosomal RNA (rRNA) gene amplicon sequencing and gas chromatography-mass spectrometry (GC-MS).</p><p><strong>Key results: </strong>The ART group showed decreased alpha diversity (the species richness or evenness in a sample). The principal coordinates analysis (a method of analysing beta diversity) showed significant difference in gut microbiota between the ART group versus the SP group (unweighted UniFrac distance, R 2 =0.04, P =0.003). Proteobacteria , Blautia and Escherichia-Shigella were enriched in the ART group, whereas the relative abundance of beneficial intestinal bacteria Faecalibacterium was lower than in the SP group. Different modes of conception were associated with several SCFAs (valeric acid (r =-0.280; P =0.017); isocaproic acid (r =-0.330; P =0.005); caproic acid (r =-0.336; P =0.004)). Significantly different SCFAs between the two groups were synchronously associated with the differential gut microbiota.</p><p><strong>Conclusions: </strong>The diversity and abundance of gut microbiota and the levels of SCFAs in women undergoing ART decreased.</p><p><strong>Implications: </strong>The application of ART shaped the microbial composition and metabolism, which may provide critical information for understanding the biological changes that occur in women with assisted reproduction.</p>","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139522016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of COVID-19 disease on the male factor in reproductive medicine - how-to advise couples undergoing IVF/ICSI.","authors":"Julia Stark, Stefan P Kuster, Vera Hungerbühler","doi":"10.1071/RD23205","DOIUrl":"10.1071/RD23205","url":null,"abstract":"<p><strong>Context: </strong>The COVID-19 pandemic has caused widespread concern about its potential impact on various aspects of human health.</p><p><strong>Aims: </strong>This narrative review aims to summarise the current knowledge about the impact of COVID-19 on sperm quality and its effect on assisted reproductive technology.</p><p><strong>Methods: </strong>In this narrative review, a literature search using the PubMed and MEDLINE databases was conducted to identify relevant original research articles published up to 29 January 2023.</p><p><strong>Results: </strong>Thirty original studies were included in our review. There is evidence that SARS-CoV-2 is detectable in seminal fluid during the acute phase of infection and for up to 1month. However, the fact that SARS-CoV-2 is barely detectable in semen makes sexual transmission very unlikely. COVID-19 infection has been associated with the following changes in sperm quality: morphology, altered motility, changed DNA fragmentation-index (DFI), decreased sperm concentration, lower total number of sperm, and a significant increase in leukocytes and cytokines. The effects mostly seem to be reversible and have not been shown to negatively affect the outcome of assisted reproductive technology but should lead to further research concerning the health of the offspring, because a correlation of increased DFI after COVID-19 even 5months after disease could be assumed.</p><p><strong>Conclusions: </strong>The findings of this narrative review suggest that SARS-CoV-2 may harm sperm quality in the acute phase.</p><p><strong>Implications: </strong>A recovery time of at least 3months regarding assisted reproductive therapy could be reasonable.</p>","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139379116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mouse <i>Tspyl5</i> promotes spermatogonia proliferation through enhancing <i>Pcna</i>-mediated DNA replication.","authors":"Xiangyou Leng, Shengyu Xie, Dachang Tao, Zhaokun Wang, Jiaying Shi, Ming Yi, Xiaolan Tan, Xinyue Zhang, Yunqiang Liu, Yuan Yang","doi":"10.1071/RD23042","DOIUrl":"10.1071/RD23042","url":null,"abstract":"<p><strong>Context: </strong>The human TSPY1 (testis-specific protein, Y-linked 1) gene is critical for spermatogenesis and male fertility. However, there have been difficulties with studying the mechanism underlying its function, partly due to the presence of the Tspy1 pseudogene in mice.</p><p><strong>Aims: </strong>TSPYL5 (TSPY-like 5), an autosomal homologous gene of TSPY1 showing a similar expression pattern in both human and mouse testes, is also speculated to play a role in male spermatogenesis. It is beneficial to understand the role of TSPY1 in spermatogenesis by investigating Tspyl5 functions.</p><p><strong>Methods: </strong>Tspyl5 -knockout mice were generated to investigate the effect of TSPYL5 knockout on spermatogenesis.</p><p><strong>Key results: </strong>Tspyl5 deficiency caused a decline in fertility and decreased the numbers of spermatogonia and spermatozoa in aged male mice. Trancriptomic detection of spermatogonia derived from aged Tspyl5 -knockout mice revealed that the Pcna -mediated DNA replication pathway was downregulated. Furthermore, Tspyl5 was proven to facilitate spermatogonia proliferation and upregulate Pcna expression by promoting the ubiquitination-degradation of the TRP53 protein.</p><p><strong>Conclusions: </strong>Our findings suggest that Tspyl5 is a positive regulator for the maintenance of the spermatogonia pool by enhancing Pcna -mediated DNA replication.</p><p><strong>Implications: </strong>This observation provides an important clue for further investigation of the spermatogenesis-related function of TSPY1 .</p>","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139379117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocrine changes induced by GnRH immunisation and subsequent early re-stimulation of testicular function with a GnRH agonist in stallions.","authors":"Camille Gautier, Jörg Aurich, Maria Melchert, Lisa-Hélène Wagner, Martim Kaps, Carolina T C Okada, Reinhard Ertl, Ingrid Walter, Christine Aurich","doi":"10.1071/RD23185","DOIUrl":"10.1071/RD23185","url":null,"abstract":"<p><strong>Context: </strong>Resumption of testicular function after gonadotrophin-releasing hormone (GnRH) immunisation varies among individual animals and some stallions regain fertility only after a prolonged time.</p><p><strong>Aims: </strong>This study evaluated endocrine effects of GnRH immunisation and early subsequent re-stimulation with a GnRH agonist. We hypothesised that GnRH agonist treatment advances resumption of normal endocrine function in GnRH-vaccinated stallions.</p><p><strong>Methods: </strong>Shetland stallions were assigned to an experimental and a control group (n =6 each). Experimental stallions were GnRH-immunised twice, 4weeks apart. Each experimental stallion was hemicastrated together with an age-matched control animal when testosterone concentration decreased below 0.3ng/mL. Three weeks later, daily treatment with the GnRH agonist buserelin was initiated (4μg/day for 4weeks followed by 8μg/day). The remaining testicle was removed when testosterone concentration exceeded 0.5ng/mL in vaccinated stallions. Blood was collected for LH, FSH, oestradiol and anti-müllerian hormone (AMH) analyses, and testicular and epididymal tissue were conserved for real-time qPCR and histology.</p><p><strong>Key results: </strong>GnRH vaccination reduced blood concentrations of LH and FSH, with a structural deterioration of testicular tissue and disruption of spermatogenesis. Daily buserelin treatment for approximately 60days partially restored gonadotropin secretion and induced a recovery of the functional organisation of the testicular tissue with effective spermatogenesis.</p><p><strong>Conclusions: </strong>Endocrine testicular function can be restored in GnRH-vaccinated stallions by daily low-dose buserelin treatment. The buserelin treatment protocol may potentially be improved regarding the dose, interval and duration.</p><p><strong>Implications: </strong>Daily buserelin treatment can be recommended for treatment of GnRH-vaccinated stallions with prolonged inhibition of testicular function.</p>","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139492333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Corrigendum to</i>: 65 Functional ablation of pregnancy-associated glycoprotein 7 affects attachment and growth of trophectoderm cell lines.","authors":"E Moreno, M S Ortega, K G Pohler","doi":"10.1071/RDv36n2Ab65_CO","DOIUrl":"10.1071/RDv36n2Ab65_CO","url":null,"abstract":"","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"36 3","pages":"NULL"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139548083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Corrigendum to</i>: 43 Correlates of reproductive tract anatomy and uterine histomorphometrics with fertility in swine.","authors":"J D'Ambrosio, M Malopolska, R Tuz, T Schwarz, D L Ekanayake, B Ahmadi, J Nowicki, E Tomaszewska, M Grzesiak, P Bartlewski","doi":"10.1071/RDv33n2Ab43_CO","DOIUrl":"10.1071/RDv33n2Ab43_CO","url":null,"abstract":"","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"33 ","pages":"682"},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140874401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Corrigendum to</i>: Efficacy of short-term cold storage prior to cryopreservation of spermatozoa in a threatened lizard.","authors":"Lachlan Campbell, John Clulow, Belinda Howe, Rose Upton, Sean Doody, Simon Clulow","doi":"10.1071/RD20231_CO","DOIUrl":"10.1071/RD20231_CO","url":null,"abstract":"<p><p>Assisted reproductive technologies (ARTs) have a significant role to play in reptile conservation, yet are severely lacking. Previous attempts to cryopreserve spermatozoa in the threatened lizard Varanus panoptes achieved approximately 48% motile sperm post-thaw for samples frozen immediately after collection. However, the feasibility of extended cold storage before cryopreservation has not been tested. We held V. panoptes spermatozoa at either 25°C or 4°C for 8 days, assessing sperm motility at days 1, 2, 4 and 8. Subsamples were cryopreserved on days 1 and 4 following the previously reported protocol for this species. Percentage motility decreased rapidly at 25°C, but did not decrease significantly until 4 days after collection at 4°C, with >30% motility maintained after 8 days. There was no significant difference in post-thaw motility or viability of samples cryopreserved after 1 or 4 days storage at 4°C, yielding substantial results for both parameters (mean motility 23.8% and 28.1% and mean viability 50.1% and 57.5% after 1 and 4 days respectively). We demonstrate the capacity to extend sperm viability for up to 8 days in unfrozen samples and to produce acceptable post-thaw motility in samples frozen after 4 days of storage, contributing to the development of valuable ARTs for lizards and other reptiles.</p>","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"33 ","pages":"619"},"PeriodicalIF":0.0,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140874307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}