The spatio-temporal distribution of aromatase cytochrome in ovary throughout the canine oestrous cycle.

IF 2.1
L Lindh, M P Kowalewski, S K Goericke-Pesch, H Lindeberg, G Schuler, O A T Peltoniemi
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Abstract

Context: New animal welfare legislation and ethical guidelines encourage alternative approaches for canine contraception, instead of surgical gonadectomy which is considered invasive and unjustified in healthy dogs.

Aims: Reversible contraception might be achieved by inhibition of aromatase (CYP19), an enzyme catalysing the conversion of androgens to oestrogens. This study provides insights into the spatio-temporal expression and distribution of aromatase in canine ovarian tissue.

Methods: Ovarian tissue was collected from 39 healthy and sexually mature bitches during different stages of the oestrous cycle: pro-oestrus (n =8), oestrus (n =12), dioestrus (n =9) (luteal phase) and anoestrus (n =10). Localisation of cytochrome P450 aromatase was determined by immunohistochemistry.

Key results: Aromatase activity in the dog is high during pro-oestrus, ovulation and early dioestrus. Comparing types of follicles and corpora lutea, the highest aromatase abundance was found in antral follicles and luteinising follicles, whereas corpora lutea and early antral follicles showed an intermediate presence of the enzyme. Interesting was the high abundance of aromatase in luteinising theca interna cells, prevailing over granulosa cells.

Conclusions and implications: Understanding of cells involved in oestradiol production is important for targeted inhibition of oestradiol synthesis, possibly offering an approach for contraception and suppression of oestrus.

犬整个发情周期卵巢中芳香化酶细胞色素的时空分布。
背景:目的:通过抑制芳香化酶(CYP19)--一种催化雄激素向雌激素转化的酶--可实现可逆性避孕。本研究有助于深入了解芳香化酶在犬卵巢组织中的时空表达和分布情况:方法:在发情周期的不同阶段收集了 39 只健康的性成熟母犬的卵巢组织:发情前期(8 只)、发情期(12 只)、二发情期(9 只)(黄体期)和无发情期(10 只)。通过免疫组化确定细胞色素 P450 芳香化酶的位置:主要结果:狗的芳香化酶活性在发情前期、排卵期和发情早期很高。比较卵泡和黄体的类型,发现前叶卵泡和黄体卵泡中的芳香化酶含量最高,而黄体和早期前叶卵泡中的芳香化酶含量居中。有趣的是,黄体化的阴道细胞中芳香化酶的含量很高,高于颗粒细胞:结论:了解参与雌二醇生成的细胞对于有针对性地抑制雌二醇合成非常重要,这可能为避孕和抑制发情提供了一种方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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