Reproduction, fertility, and development最新文献_第6页

Molecular dynamics simulations of cytochrome P450 aromatases reveal structural variances across the cat family. 细胞色素P450芳香酶的分子动力学模拟揭示了猫家族的结构差异。

IF 2.1

Reproduction, fertility, and development Pub Date : 2025-08-01 DOI: 10.1071/RD25062

Rageshwari R Marolikar, Paul D O'Leary, Ajay Singh Panwar, Lisandra L Martin

{"title":"Molecular dynamics simulations of cytochrome P450 aromatases reveal structural variances across the cat family.","authors":"Rageshwari R Marolikar, Paul D O'Leary, Ajay Singh Panwar, Lisandra L Martin","doi":"10.1071/RD25062","DOIUrl":"https://doi.org/10.1071/RD25062","url":null,"abstract":"Context Aromatase (CYP19A1) is a key enzyme in steroidogenesis, converting androgens to oestrogens, essential for reproductive function in vertebrates. While human aromatase has been extensively studied, comparative analyses in mammals, particularly felids, remain limited. Aims This study investigates the structural and functional dynamics of aromatase in various cat species, including the extinct Homotherium latidens and extant species such as Panthera tigris , Puma concolor , Acinonyx jubatus , and Felis catus . The goal is to assess evolutionary differences affecting dimerisation and enzymatic activity. Methods Homology models of feline aromatase were built using the human aromatase crystal structure as a template. Molecular dynamics (MD) simulations were conducted in both solvent and membrane environments to evaluate dimer stability, electrostatic interactions, and haem cofactor retention. Key results Sequence analysis showed over 99% conservation within felids and ~86% identity with human aromatase, with 69 key residue differences. MD simulations revealed that substitutions at the dimerisation interface weakened electrostatic interactions, reducing dimer stability in felids compared to humans. Membrane embedding improved stability, particularly in human aromatase, due to strong hydrogen-bonding interactions. Conclusions Evolutionary divergence has altered dimerisation stability in feline aromatases, potentially influencing enzymatic function. Reduced dimer formation may impact substrate binding and catalytic efficiency. Implications These findings provide insights into aromatase evolution and function, offering a foundation for future research into species-specific steroid biosynthesis and potential drug design strategies.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"37 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145115954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modelling menstruation in the common mouse: a narrative review. 普通小鼠月经模型：叙述性回顾。

IF 2.1

Reproduction, fertility, and development Pub Date : 2025-08-01 DOI: 10.1071/RD25055

Laura M Rogers, Gendie E Lash, Greg M Anderson, Jane E Girling

{"title":"Modelling menstruation in the common mouse: a narrative review.","authors":"Laura M Rogers, Gendie E Lash, Greg M Anderson, Jane E Girling","doi":"10.1071/RD25055","DOIUrl":"https://doi.org/10.1071/RD25055","url":null,"abstract":"Despite occurring in up to 50% of the human population, menstruation is a fundamentally understudied process with limited treatment options when menstrual pathologies arise. Reasons for this deficit include the inherent ethical and technical constraints associated with researching menstruation. The multifactorial nature of many menstrual-related pathologies means in vivo research is necessary; however, this type of research is difficult in humans, and non-human species that menstruate naturally are often not suitable as research models. Consequently, most menstrual research relies on an artificially induced menstrual-like process in the non-menstruating laboratory mouse. This review investigates mouse models of menstruation and how specific technical variables are used to produce or modulate a menstrual-like process. The review describes two key categories of models, those that are ovariectomy-based versus those that are pseudopregnancy-based. The menstrual-like process occurring in these models varied slightly;the underlying reason for the variation is likely to be the method of progesterone withdrawal. Models that withdrew progesterone specifically had a far less rapid endometrial breakdown in comparison to those that withdrew all ovarian input. These outcomes suggest that a loss of ovarian factors other than progesterone is likely impacting the breakdown process. The review highlights the gaps in our understanding of the mechanisms of endometrial breakdown and repair in these proxies for menstruation and the subsequent impacts on any conclusions drawn from these models.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"37 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of irisin in the porcine pituitary gland during the oestrous cycle and early pregnancy: the role of GnRH, gonadotropins, and insulin. 发情周期和妊娠早期猪脑垂体中鸢尾素的表达：GnRH、促性腺激素和胰岛素的作用。

IF 2.1

Reproduction, fertility, and development Pub Date : 2025-08-01 DOI: 10.1071/RD25057

Barbara Zarzecka, Kamil Dobrzyn, Marta Kiezun, Grzegorz Kopij, Monika Dawid, Agnieszka Rak, Cecilia Dall'Aglio, Tadeusz Kaminski, Nina Smolinska

{"title":"Expression of irisin in the porcine pituitary gland during the oestrous cycle and early pregnancy: the role of GnRH, gonadotropins, and insulin.","authors":"Barbara Zarzecka, Kamil Dobrzyn, Marta Kiezun, Grzegorz Kopij, Monika Dawid, Agnieszka Rak, Cecilia Dall'Aglio, Tadeusz Kaminski, Nina Smolinska","doi":"10.1071/RD25057","DOIUrl":"https://doi.org/10.1071/RD25057","url":null,"abstract":"Context Metabolic status significantly affects female reproductive function, with both excess and deficiency of body fat negatively affecting fertility. Irisin, a hormone secreted by muscle and fat tissue, is linked to metabolism and reproduction, but its role in the pituitary gland remains unclear. Aims This study investigated the expression of irisin and its receptor (integrin αV/β5) in the anterior (AP) and posterior (PP) lobes of the porcine pituitary during the oestrous cycle and early pregnancy. We hypothesised that they are localised in specific pituitary cell types and that gonadotropin-releasing hormone (GnRH), luteinising hormone (LH), follicle-stimulating hormone (FSH), and insulin modulate irisin expression and secretion by AP cells. Methods The expression of irisin and integrin αV/β5 was analysed using quantitative real-time polymerase chain reaction (qPCR) and western blotting. Immunofluorescence was used to determine colocalisation with pituitary hormones. AP cells were cultured in vitro and treated with GnRH, LH, FSH, or insulin to assess their effects on irisin protein concentrations and secretion. Key results Irisin and its receptor were expressed in both AP and PP lobes and colocalised with all major trophic cell types. Their expression varied depending on the reproductive stage. GnRH, LH, FSH, and insulin inhibited irisin secretion by AP cells during the luteal phase, whereas only insulin had an effect during the follicular phase. Conclusions Irisin and its receptor are expressed in a hormone-dependent manner and localise to specific pituitary cell types, suggesting intra-pituitary regulatory roles. Implications These findings indicated that irisin may act as a local modulator of pituitary function and reproductive hormone regulation, linking metabolic and reproductive health.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"37 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive proteomic characterization and functional annotation of common carp seminal plasma. 鲤鱼精浆综合蛋白质组学特征及功能注释。

IF 2.1

Reproduction, fertility, and development Pub Date : 2025-08-01 DOI: 10.1071/RD25034

Anna Malgorzata Majewska, Natalia Kodzik, Mariola Aleksandra Dietrich, Andrzej Ciereszko

{"title":"Comprehensive proteomic characterization and functional annotation of common carp seminal plasma.","authors":"Anna Malgorzata Majewska, Natalia Kodzik, Mariola Aleksandra Dietrich, Andrzej Ciereszko","doi":"10.1071/RD25034","DOIUrl":"https://doi.org/10.1071/RD25034","url":null,"abstract":"Context Understanding the protein composition of seminal plasma is crucial for elucidating reproductive mechanisms and improving aquaculture practices. Proteomic studies provide insights into the biological functions of seminal plasma in fish, yet comprehensive datasets for carp remain limited. Aims This study aimed to comprehensively characterize the proteome of carp seminal plasma, classify identified proteins on the basis of their cellular localization, and explore their functional roles in reproductive and physiological processes. Methods Using high-throughput liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis and updated Cyprinus carpio genome annotation, we identified and classified proteins on the basis of homology to human genes and fish-specific annotations. Bioinformatic tools were employed to analyze their functions and involvement in key biological pathways. Key results In total, 1402 proteins were identified, the highest number being reported for this species. Of the 1354 proteins homologous to human genes, 141 were secretory, 92 were both secretory and intracellular, and 1121 were intracellular. Additionally, 49 proteins were fish-specific, involved in immune response, detoxification, cold protection, and proteolytic defence. Bioinformatic analyses indicated roles in immune and stress responses, extracellular matrix organization, metabolism, and gene expression. The presence of extracellular vesicles was supported by the identification of 636 associated proteins. Reproductive-related proteins were linked to gamete generation, spermatogenesis, and sperm motility. Conclusions This dataset represents the most comprehensive proteomic profile of carp seminal plasma, offering new insights into its biological functions. Implications The findings enhance our understanding of carp reproductive biology and have potential applications in aquaculture, including sperm preservation, fertilization success, and disease resistance.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"37 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145115944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Technical report on preservation of dromedary camel embryos at 4°C for up to 72 h in catalase-supplemented media. 单峰骆驼胚胎在补充过氧化氢酶的培养基中在4°C下保存72小时的技术报告。

IF 2.1

Reproduction, fertility, and development Pub Date : 2025-07-01 DOI: 10.1071/RD25030

Julian Alexandra Skidmore, Brendan Patrick Mulligan, Jane Louise Vaughan

{"title":"Technical report on preservation of dromedary camel embryos at 4°C for up to 72 h in catalase-supplemented media.","authors":"Julian Alexandra Skidmore, Brendan Patrick Mulligan, Jane Louise Vaughan","doi":"10.1071/RD25030","DOIUrl":"https://doi.org/10.1071/RD25030","url":null,"abstract":"Context Catalase, an antioxidant, prolonged camel sperm survival during storage at 5°C; however, its effect on storage of camel embryos at 4°C is unknown. Aims This study aims to evaluate the possibility of improving pregnancy rates for embryos stored at 4°C for 24-72h in catalase-supplemented media. Methods Embryos recovered from camels flushed 8days after mating were deposited in Eppendorf tubes containing embryo holding media, either supplemented with 500 IU catalase (Group 1) or without supplementation (Group 2). These Eppendorf tubes were placed in an Equitainer and cooled to 4°C. After 24h, 11 embryos in each group were transferred into recipients 7days after gonadotropin-releasing hormone injection, and the remainder was placed in the fridge at 4°C for a further 24 (n =11/group) or 48h (n =11/group) before transfer. Key results A non-significant increase in pregnancy rate was achieved from embryos cooled in media containing catalase compared with the controls at 24 and 48h, although there was no difference at 72h (9/11 (82%) vs 5/11 (45%), 7/11 (64%) vs 2/11 (18%) and 2/11 (18%) vs 2/11 (18%), for with catalase vs controls at 24, 48 or 72h respectively). Conclusions These results showed that there was a tendency for improved pregnancy rates at 24 (82% vs 45%) and 48h (64% vs 18%) of cooling in catalase-supplemented media compared with controls. This improvement was not evident at 72h. Implications The ability to keep embryos at 4°C for 24-48h reduces the need for such tight synchronization between donors and recipients.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"37 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145115928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factor-mediated gene regulatory networks in the formation of oocytes. 卵母细胞形成过程中转录因子介导的基因调控网络。

IF 2.1

Reproduction, fertility, and development Pub Date : 2025-07-01 DOI: 10.1071/RD25054

Di Wu, Zifan Liang, Ziqi Li, Boyang Zhang, Qiwen Li, Kesong Shi, Shu Fang

{"title":"Transcription factor-mediated gene regulatory networks in the formation of oocytes.","authors":"Di Wu, Zifan Liang, Ziqi Li, Boyang Zhang, Qiwen Li, Kesong Shi, Shu Fang","doi":"10.1071/RD25054","DOIUrl":"10.1071/RD25054","url":null,"abstract":"Context The induction of oocytes from embryonic stem cells (ESCs) in vitro provides a promising tool for the treatment of female infertility. Various molecules are involved in this complex process, which requires further elucidation. Aims This study aims to screen for factors that induce the differentiation of ESCs into oocytes in vitro by constructing transcription factor (TF)-mediated gene regulatory networks (GRNs) during the formation of oocytes. Methods Based on publicly available multi-omics data, the weighted gene co-expression network analysis (WGCNA) method identified oocyte-specific TFs and key oocyte-specific genes. Additionally, chromatin immunoprecipitation (ChIP) sequencing data and ChIP-qPCR analysis were used to examine GRNs mediated by oocyte-specific TFs. Key results First, by analyzing assay for transposase-accessible chromatin sequencing (ATAC-seq) and DNase I hypersensitive site sequencing (DNase-seq) data from human and mouse ESCs, primordial germ cells (PGCs), and oocytes, we identified five and three oocyte-specific TFs, respectively. RNA sequencing and WGCNA further revealed 38 key oocyte-specific genes. Subsequently, when comparing cell-specific TFs in mouse and human oocytes, we identified three overlapping oocyte-specific TFs (NFYA, NFYB, and NFYC). Notably, NFYA exhibited significantly elevated expression levels in oocytes compared to ESCs and PGCs. Additionally, ChIP-qPCR results demonstrated that NFYA was relatively enriched at the promoter region of the key oocyte-specific gene, m6 A demethylase Alkbh5 . Conclusions This study provides preliminary insights into the role of cell-specific TFs and TF-mediated GRNs in oocyte formation by identifying oocyte-specific genes and key oocyte-specific TFs. Implications The findings indicate that their intricate regulatory mechanisms may significantly contribute to enhancing the efficiency of differentiating ESCs into oocytes.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"37 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-NGF and its receptors are present in ram sperm cells, but β-NGF was undetectable in seminal plasma. β-NGF及其受体存在于公羊精细胞中，但在精浆中未检测到β-NGF。

IF 2.1

Reproduction, fertility, and development Pub Date : 2025-07-01 DOI: 10.1071/RD25060

Javier Meilán, Rodrigo Carrasco, Marcelo Ratto, Rodolfo Ungerfeld

{"title":"β-NGF and its receptors are present in ram sperm cells, but β-NGF was undetectable in seminal plasma.","authors":"Javier Meilán, Rodrigo Carrasco, Marcelo Ratto, Rodolfo Ungerfeld","doi":"10.1071/RD25060","DOIUrl":"https://doi.org/10.1071/RD25060","url":null,"abstract":"Context In males, the nerve growth factor β (β-NGF), along with its receptors TrkA and p75, is closely related to semen traits and quality in certain species. However, to the best of our knowledge, no study has addressed either the existence of this neurotrophin and its receptors on ram sperm cells or the relationship between β-NGF and semen traits. Aims The aim of this study was to determine the expression of β-NGF and its receptors in spermatozoa and to relate the concentration of β-NGF in seminal plasma to sperm morphology and quality in rams. Methods The expression of β-NGF and its receptors in sperm cells was performed using immunofluorescence, and the concentration of β-NGF in semen was determined by enzyme-linked immunosorbent assay. Key results β-NGF, TrkA and p75 were detected in the spermatozoa of the ram; however, β-NGF was not detectable in the seminal plasma. Conclusions This study provides evidence, for the first time, of the presence of β-NGF and its receptors TrkA and p75, in spermatozoa of rams. However, it was undetectable in seminal plasma collected during the transition from the breeding to the non-breeding season. Detecting and quantifying β-NGF in ram seminal plasma presents several challenges, primarily due to its low concentrations and the techniques employed. Implications These findings indicate that rams, among the males, have β-NGF and its receptors present in sperm cells, making them another candidate species to expand our knowledge of this important protein in mammalian reproduction.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"37 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145115986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole-exome sequencing reveals a novel mutation in the SUN5 gene causing acephalic spermatozoa syndrome. 全外显子组测序揭示了导致头型精子综合征的SUN5基因的新突变。

IF 2.1

Reproduction, fertility, and development Pub Date : 2025-07-01 DOI: 10.1071/RD25058

Seyedeh-Hanieh Hosseini, Nastaran Salehisedeh, Mahsa Allahgholi, Ali Ahani, Mohammad Ali Sadighi Gilani, Marjan Sabbaghian

{"title":"Whole-exome sequencing reveals a novel mutation in the SUN5 gene causing acephalic spermatozoa syndrome.","authors":"Seyedeh-Hanieh Hosseini, Nastaran Salehisedeh, Mahsa Allahgholi, Ali Ahani, Mohammad Ali Sadighi Gilani, Marjan Sabbaghian","doi":"10.1071/RD25058","DOIUrl":"10.1071/RD25058","url":null,"abstract":"Context Acephalic spermatozoa syndrome (ASS) is one of the most severe male spermatogenic disorders, featuring a large number of headless spermatozoa in the ejaculate. Although genetic factors play an important role in spermatogenesis, only a few genes are correlated with sperm defects and male infertility. Studies have revealed that genetic mutations are the main causes of ASS. Therefore, finding new genes that lead to ASS is significant in choosing the correct treatment methods and genetic counseling for these patients.p Aims This study aimed to identify the genetic causes of ASS in two infertile brothers whose parents are first cousins. Methods Whole-exome sequencing (WES) was performed using peripheral blood genomic DNA. PCR reactions, Sanger sequencing, and immunocytochemistry were performed to confirm the results of WES. Key results We identified a novel homozygous mutation in the SUN5 gene (NM_080675: exon11: c.879dupc: p.k), and Sanger sequencing confirmed our finding. There was no signal of SUN5-antibody in the protein assessment of the spermatozoa from our patient. We conducted two intracytoplasmic sperm injections (ICSI) cycles for the proband, however, the treatment did not result in his partner achieving pregnancy. Conclusions Our findings suggest that the novel mutation of the SUN5 gene is responsible for ASS. Implications These results highlight the diagnostic value of identifying SUN5 mutations in in patients with ASS. The current findings contribute to a better understanding of the genetic basis of ASS and can inform future clinical decisions as more data become available.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"37 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145115959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Markers for early pregnancy prediction in Zebu cows subjected to timed artificial insemination. 定时人工授精对瘤牛早孕预测的标记。

IF 2.1

Reproduction, fertility, and development Pub Date : 2025-07-01 DOI: 10.1071/RD25042

Mariana Alves de Andrade Silva, Artur Azevedo Menezes, Endrigo Adonis Braga de Araujo, Marcos Chalhoub Coelho Lima, Rodrigo Freitas Bittencourt, Marcos Vinícius Galvão Loiola, Antonio de Lisboa Ribeiro Filho

{"title":"Markers for early pregnancy prediction in Zebu cows subjected to timed artificial insemination.","authors":"Mariana Alves de Andrade Silva, Artur Azevedo Menezes, Endrigo Adonis Braga de Araujo, Marcos Chalhoub Coelho Lima, Rodrigo Freitas Bittencourt, Marcos Vinícius Galvão Loiola, Antonio de Lisboa Ribeiro Filho","doi":"10.1071/RD25042","DOIUrl":"https://doi.org/10.1071/RD25042","url":null,"abstract":"Context To increase the number of cows pregnant in the early breeding season, strategies have been developed that seek to reduce the interval for pregnancy diagnosis. Aims The aim of this study was to evaluate markers for the early detection of pregnancy in Zebu cows. Methods A total of 175 Zebu cows were assessed at the time of insemination for the follicle diameter (FOLD), follicular wall area (FOLA) and follicle blood flow (FOLV). Twenty days after fixed-time artificial insemination (TAI), the corpus luteum was evaluated for diameter (CLD), area (CLA), vascularized area (CLV), and percentage of vascularization (%CLV). Blood samples were also collected to measure progesterone levels 20 days after insemination (P4D20). On the basis of FOLD, FOLV, CLD, CLV, and P4D20 as potential early pregnancy markers, the animals were categorized into two groups: negative predictive diagnosis of pregnancy (NPD) and positive predictive diagnosis (PPD). A definitive pregnancy diagnosis was performed 30days post-TAI. Key results Pregnant cows exhibited higher FOLD, FOLA, FOLV, CLD, CLA, CLV, %CLV, and P4D20 levels compared to non-pregnant cows. Significant correlations were found between FOLD and FOLA, FOLV, percentage of follicular vascularization (FOLV%), CLD, CLA, CLV, %CLV, and P4D20. FOLA was also significantly correlated with FOLV, CLD, CLA, CLV, %CLV, and P4D20. Similarly, FOLV was significantly correlated with FOLV%, CLD, CLA, CLV, %CLV, and P4D20. CLD correlated significantly with CLA, CLV, %CLV, and P4D20, while CLV showed significant correlations with %CLV and P4D20. Conclusions The follicular and luteal morphofunctional characteristics are intricately linked to pregnancy establishment. Implications The CLV emerged as a promising marker for achieving earlier pregnancy diagnoses.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"37 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial lipopolysaccharide (LPS) impairs sperm mitochondrial membrane potential and induces sperm apoptosis in infertile men. 细菌脂多糖（LPS）损害不育男性精子线粒体膜电位并诱导精子凋亡。

IF 2.1

Reproduction, fertility, and development Pub Date : 2025-07-01 DOI: 10.1071/RD25069

Sana Sahnoun, Afifa Sellami, Hela Menif, Nour Louati, Salima Daoud, Hanen Sellami, Saloua Lassoued

{"title":"Bacterial lipopolysaccharide (LPS) impairs sperm mitochondrial membrane potential and induces sperm apoptosis in infertile men.","authors":"Sana Sahnoun, Afifa Sellami, Hela Menif, Nour Louati, Salima Daoud, Hanen Sellami, Saloua Lassoued","doi":"10.1071/RD25069","DOIUrl":"https://doi.org/10.1071/RD25069","url":null,"abstract":"Context Lipopolysaccharide (LPS), a major component of Gram-negative bacteria membrane, is widely implicated in the pathogenesis of male reproductive system infections. Aims This study aims to elucidate the LPS effect on sperm motility, mitochondrial membrane potential (ΔΨm) and apoptosis in human sperms. Methods Our experimental study was conducted on 34 sperm samples from male partners of infertile couples divided into fertile group (n =8) and infertile group (n =26). For each sample, two sperm suspensions were prepared, namely, a control suspension (non-treated with LPS) and an LPS-treated suspension (200ng/mL). The two suspensions were incubated for 18h at room temperature. Sperm motility was evaluated by microscopic observation, whereas sperm mitochondrial membrane potential and apoptosis were assessed using flow cytometry after JC-10 and 7-AAD staining. Key results In fertile group, no significant differences in sperm motility, sperm ΔΨm and apoptotic sperm rates were detected after incubation with LPS. However, in infertile group, the LPS reduced sperm motility significantly and induced significant decrease in the rates of sperm with high ΔΨm. Furthermore, a significant decrease in viable sperm rates was detected concomitantly with a significant increase of apoptotic sperm rates. Conclusions This study showed that LPS may impair sperm mitochondrial membrane potential and lead to the activation of some pathways responsible for sperm apoptosis in infertile men. Implications This study has contributed to better understanding of the mechanisms underlying sperm disorders induced by bacterial endotoxins, in particular LPS, especially in infertile men.","PeriodicalId":516117,"journal":{"name":"Reproduction, fertility, and development","volume":"37 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0