Plant Reproduction最新文献_第8页

The TCP transcription factor HvTB2 heterodimerizes with VRS5 and controls spike architecture in barley. TCP转录因子HvTB2与VRS5异源二聚体并控制大麦穗结构。

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2022-09-01 DOI: 10.1007/s00497-022-00441-8

Tatiana de Souza Moraes, Sam W van Es, Inmaculada Hernández-Pinzón, Gwendolyn K Kirschner, Froukje van der Wal, Sylvia Rodrigues da Silveira, Jacqueline Busscher-Lange, Gerco C Angenent, Matthew Moscou, Richard G H Immink, G Wilma van Esse

{"title":"The TCP transcription factor HvTB2 heterodimerizes with VRS5 and controls spike architecture in barley.","authors":"Tatiana de Souza Moraes, Sam W van Es, Inmaculada Hernández-Pinzón, Gwendolyn K Kirschner, Froukje van der Wal, Sylvia Rodrigues da Silveira, Jacqueline Busscher-Lange, Gerco C Angenent, Matthew Moscou, Richard G H Immink, G Wilma van Esse","doi":"10.1007/s00497-022-00441-8","DOIUrl":"https://doi.org/10.1007/s00497-022-00441-8","url":null,"abstract":"Key message: Understanding the molecular network, including protein-protein interactions, of VRS5 provide new routes towards the identification of other key regulators of plant architecture in barley. The TCP transcriptional regulator TEOSINTE BRANCHED 1 (TB1) is a key regulator of plant architecture. In barley, an important cereal crop, HvTB1 (also referred to as VULGARE SIX-ROWED spike (VRS) 5), inhibits the outgrowth of side shoots, or tillers, and grains. Despite its key role in barley development, there is limited knowledge on the molecular network that is utilized by VRS5. In this work, we performed protein-protein interaction studies of VRS5. Our analysis shows that VRS5 potentially interacts with a diverse set of proteins, including other class II TCP's, NF-Y TF, but also chromatin remodelers. Zooming in on the interaction capacity of VRS5 with other TCP TFs shows that VRS5 preferably interacts with other class II TCP TFs in the TB1 clade. Induced mutagenesis through CRISPR-Cas of one of the putative VRS5 interactors, HvTB2 (also referred to as COMPOSITUM 1 and BRANCHED AND INDETERMINATE SPIKELET 1), resulted in plants that have lost their characteristic unbranched spike architecture. More specifically, hvtb2 mutants exhibited branches arising at the main spike, suggesting that HvTB2 acts as inhibitor of branching. Our protein-protein interaction studies of VRS5 resulted in the identification of HvTB2 as putative interactor of VRS5, another key regulator of spike architecture in barley. The study presented here provides a first step to underpin the protein-protein interactome of VRS5 and to identify other, yet unknown, key regulators of barley plant architecture.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10425231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Effect of assimilate competition during early seed development on the pod and seed growth traits in soybean. 种子早期发育过程中的同素竞争对大豆豆荚和种子生长性状的影响

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2022-09-01 Epub Date: 2022-03-02 DOI: 10.1007/s00497-022-00439-2

Mohammad Foteh Ali, Paige Brown, John Thomas, Montserrat Salmerόn, Tomokazu Kawashima

{"title":"Effect of assimilate competition during early seed development on the pod and seed growth traits in soybean.","authors":"Mohammad Foteh Ali, Paige Brown, John Thomas, Montserrat Salmerόn, Tomokazu Kawashima","doi":"10.1007/s00497-022-00439-2","DOIUrl":"10.1007/s00497-022-00439-2","url":null,"abstract":"Although the seed remains small in size during the initial stage of seed development (the lag phase), several studies indicate that environment and assimilate supply level manipulations during the lag phase affect the final seed size. However, the manipulations were not only at the lag phase, making it difficult to understand the specific role of the lag phase in final seed size determination. It also remained unclear whether environmental cues are sensed by plants and regulate seed development or if it is simply the assimilate supply level, changed by the environment, that affects the subsequent seed development. We investigated soybean (Glycine max L. Merr.) seed phenotypes grown in a greenhouse using different source-sink manipulations (shading and removal of flowers and pods) during the lag phase. We show that assimilate supply is the key factor controlling flower and pod abortion and that the assimilate supply during the lag phase affects the subsequent potential seed growth rate during the seed filling phase. In response to low assimilate supply, plants adjust flower/pod abortion and lag phase duration to supply the minimum assimilate per pod/seed. Our results provide insight into the mechanisms whereby the lag phase is crucial for seed development and final seed size potential, essential parameters that determine yield.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10713835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Plastid ribosome protein L5 is essential for post-globular embryo development in Arabidopsis thaliana. 拟南芥质体核糖体蛋白L5对球状后胚胎发育至关重要。

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2022-09-01 DOI: 10.1007/s00497-022-00440-9

Gilles Dupouy, Emma McDermott, Ronan Cashell, Anna Scian, Marcus McHale, Peter Ryder, Joelle de Groot, Noel Lucca, Galina Brychkova, Peter C McKeown, Charles Spillane

{"title":"Plastid ribosome protein L5 is essential for post-globular embryo development in Arabidopsis thaliana.","authors":"Gilles Dupouy, Emma McDermott, Ronan Cashell, Anna Scian, Marcus McHale, Peter Ryder, Joelle de Groot, Noel Lucca, Galina Brychkova, Peter C McKeown, Charles Spillane","doi":"10.1007/s00497-022-00440-9","DOIUrl":"https://doi.org/10.1007/s00497-022-00440-9","url":null,"abstract":"Plastid ribosomal proteins (PRPs) can play essential roles in plastid ribosome functioning that affect plant function and development. However, the roles of many PRPs remain unknown, including elucidation of which PRPs are essential or display redundancy. Here, we report that the nuclear-encoded PLASTID RIBOSOMAL PROTEIN L5 (PRPL5) is essential for early embryo development in A. thaliana, as homozygous loss-of-function mutations in the PRPL5 gene impairs chloroplast development and leads to embryo failure to develop past the globular stage. We confirmed the prpl5 embryo-lethal phenotype by generating a mutant CRISPR/Cas9 line and by genetic complementation. As PRPL5 underwent transfer to the nuclear genome early in the evolution of Embryophyta, PRPL5 can be expected to have acquired a chloroplast transit peptide. We identify and validate the presence of an N-terminal chloroplast transit peptide, but unexpectedly also confirm the presence of a conserved and functional Nuclear Localization Signal on the protein C-terminal end. This study highlights the fundamental role of the plastid translation machinery during the early stages of embryo development in plants and raises the possibility of additional roles of plastid ribosomal proteins in the nucleus.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10713837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Let's shape again: the concerted molecular action that builds the pollen tube. 让我们再看一遍:形成花粉管的协调一致的分子作用。

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2022-06-01 Epub Date: 2022-01-18 DOI: 10.1007/s00497-022-00437-4

Aslıhan Çetinbaş-Genç, Veronica Conti, Giampiero Cai

{"title":"Let's shape again: the concerted molecular action that builds the pollen tube.","authors":"Aslıhan Çetinbaş-Genç, Veronica Conti, Giampiero Cai","doi":"10.1007/s00497-022-00437-4","DOIUrl":"https://doi.org/10.1007/s00497-022-00437-4","url":null,"abstract":"Key message: The pollen tube is being subjected to control by a complex network of communication that regulates its shape and the misfunction of a single component causes specific deformations. In flowering plants, the pollen tube is a tubular extension of the pollen grain required for successful sexual reproduction. Indeed, maintaining the unique shape of the pollen tube is essential for the pollen tube to approach the embryo sac. Many processes and molecules (such as GTPase activity, phosphoinositides, Ca2+ gradient, distribution of reactive oxygen species and nitric oxide, nonuniform pH values, organization of the cytoskeleton, balance between exocytosis and endocytosis, and cell wall structure) play key and coordinated roles in maintaining the cylindrical shape of pollen tubes. In addition, the above factors must also interact with each other so that the cell shape is maintained while the pollen tube follows chemical signals in the pistil that guide it to the embryo sac. Any intrinsic changes (such as erroneous signals) or extrinsic changes (such as environmental stresses) can affect the above factors and thus fertilization by altering the tube morphology. In this review, the processes and molecules that enable the development and maintenance of the unique shape of pollen tubes in angiosperms are presented emphasizing their interaction with specific tube shape. Thus, the purpose of the review is to investigate whether specific deformations in pollen tubes can help us to better understand the mechanism underlying pollen tube shape.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39828926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. 拟南芥减数分裂期间ARGONAUTE蛋白的积累动态。

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2022-06-01 Epub Date: 2021-11-23 DOI: 10.1007/s00497-021-00434-z

Cecilia Oliver, German Martinez

引用次数: 1

A semi in vivo pollination technique to assess the level of gametophytic self-incompatibility and pollen tube growth in pear (Pyrus communis L.). 梨配子体自交不亲和和花粉管生长的半体内授粉技术研究。

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2022-06-01 Epub Date: 2022-01-15 DOI: 10.1007/s00497-021-00435-y

Hanne Claessen, Bram Van de Poel, Wannes Keulemans, Nico De Storme

{"title":"A semi in vivo pollination technique to assess the level of gametophytic self-incompatibility and pollen tube growth in pear (Pyrus communis L.).","authors":"Hanne Claessen, Bram Van de Poel, Wannes Keulemans, Nico De Storme","doi":"10.1007/s00497-021-00435-y","DOIUrl":"https://doi.org/10.1007/s00497-021-00435-y","url":null,"abstract":"Key message: We describe a semi in vivo pollination technique to determine the compatibility relation between different pear cultivars. This assay provides a valuable addition to existing tools in GSI research. The gametophytic self-incompatibility (GSI) system in Pyrus inhibits fertilization by pollen that shares one of the two S-alleles of the style. Depending on their S-locus genotype, two pear cultivars therefore either show a cross-compatible, semi-compatible or incompatible interaction. Because GSI greatly influences seed and fruit set, accurate knowledge of the compatibility type of a cultivar is key for both pear fruit production and breeding. Currently, compatibility relations between different pear cultivars are generally assessed via S-genotyping. However, this approach is restricted to the currently known S-alleles in pear, and does not provide functional assessment of the level of (self-)incompatibility. We here present an optimized semi in vivo pollination assay, that enables quantitative analysis of (self-)incompatibility in pear, and that can also serve useful for more fundamental studies on pollen tube development and pollen-style interactions. This assay involves in vitro incubation of cut pollinated styles followed by microscopic counting of emerging pollen tubes at a specific time interval. The validity and selectivity of this method to determine compatibility interactions in pear is demonstrated in the cultivars \"Celina\" and \"Packham's Triumph.\" Overall, this technique constitutes a valuable tool for quantitatively determining in vivo pollen tube growth and (cross-)compatibility in pear.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39822658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

DNA Methyltransferase 3 (MET3) is regulated by Polycomb group complex during Arabidopsis endosperm development. DNA甲基转移酶3 (MET3)在拟南芥胚乳发育过程中受到Polycomb基团复合物的调控。

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2022-06-01 Epub Date: 2022-01-28 DOI: 10.1007/s00497-021-00436-x

Louis Tirot, Diane M V Bonnet, Pauline E Jullien

{"title":"DNA Methyltransferase 3 (MET3) is regulated by Polycomb group complex during Arabidopsis endosperm development.","authors":"Louis Tirot, Diane M V Bonnet, Pauline E Jullien","doi":"10.1007/s00497-021-00436-x","DOIUrl":"https://doi.org/10.1007/s00497-021-00436-x","url":null,"abstract":"Complex epigenetic changes occur during plant reproduction. These regulations ensure the proper transmission of epigenetic information as well as allowing for zygotic totipotency. In Arabidopsis, the main DNA methyltransferase is called MET1 and is responsible for methylating cytosine in the CG context. The Arabidopsis genome encodes for three additional reproduction-specific homologs of MET1, namely MET2a, MET2b and MET3. In this paper, we show that the DNA methyltransferase MET3 is expressed in the seed endosperm and its expression is later restricted to the chalazal endosperm. MET3 is biallelically expressed in the endosperm but displays a paternal expression bias. We found that MET3 expression is regulated by the Polycomb complex proteins FIE and MSI1. Seed development is not impaired in met3 mutant, and we could not observe significant transcriptional changes in met3 mutant. MET3 might regulates gene expression in a Polycomb mutant background suggesting a further complexification of the interplay between H3K27me3 and DNA methylation in the seed endosperm. KEY MESSAGE: The DNA METHYLTRANSFERASE MET3 is controlled by Polycomb group complex during endosperm development.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39866546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Passiflora organensis FT/TFL1 gene family and their putative roles in phase transition and floral initiation. 西番莲FT/TFL1基因家族及其在相变和花形成中的作用

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2022-06-01 Epub Date: 2021-11-08 DOI: 10.1007/s00497-021-00431-2

Tatiana S Moraes, Richard G H Immink, Adriana P Martinelli, Gerco C Angenent, Wilma van Esse, Marcelo C Dornelas

{"title":"Passiflora organensis FT/TFL1 gene family and their putative roles in phase transition and floral initiation.","authors":"Tatiana S Moraes, Richard G H Immink, Adriana P Martinelli, Gerco C Angenent, Wilma van Esse, Marcelo C Dornelas","doi":"10.1007/s00497-021-00431-2","DOIUrl":"https://doi.org/10.1007/s00497-021-00431-2","url":null,"abstract":"Key message: Comprehensive analysis of the FT/TFL1 gene family in Passiflora organensis results in understanding how these genes might be involved in the regulation of the typical plant architecture presented by Passiflora species. Passion fruit (Passiflora spp) is an economic tropical fruit crop, but there is hardly any knowledge available about the molecular control of phase transition and flower initiation in this species. The florigen agent FLOWERING LOCUS T (FT) interacts with the bZIP protein FLOWERING LOCUS D (FD) to induce flowering in the model species Arabidopsis thaliana. Current models based on research in rice suggest that this interaction is bridged by 14-3-3 proteins. We identified eight FT/TFL1 family members in Passiflora organensis and characterized them by analyzing their phylogeny, gene structure, expression patterns, protein interactions and putative biological roles by heterologous expression in Arabidopsis. PoFT was highest expressed during the adult vegetative phase and it is supposed to have an important role in flowering induction. In contrast, its paralogs PoTSFs were highest expressed in the reproductive phase. While ectopic expression of PoFT in transgenic Arabidopsis plants induced early flowering and inflorescence determinacy, the ectopic expression of PoTSFa caused a delay in flowering. PoTFL1-like genes were highest expressed during the juvenile phase and their ectopic expression caused delayed flowering in Arabidopsis. Our protein-protein interaction studies indicate that the flowering activation complexes in Passiflora might deviate from the hexameric complex found in the model system rice. Our results provide insights into the potential functions of FT/TFL1 gene family members during floral initiation and their implications in the special plant architecture of Passiflora species, contributing to more detailed studies on the regulation of passion fruit reproduction.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39689219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Dissection and ultramicroscopic observation of an apical pollen tube of Pyrus. 梨属植物顶端花粉管的解剖与超微观察。

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2022-03-01 Epub Date: 2021-11-03 DOI: 10.1007/s00497-021-00433-0

Chenxi Shi, Demian Wang, Yaqin Guan, Haiyong Qu

{"title":"Dissection and ultramicroscopic observation of an apical pollen tube of Pyrus.","authors":"Chenxi Shi, Demian Wang, Yaqin Guan, Haiyong Qu","doi":"10.1007/s00497-021-00433-0","DOIUrl":"https://doi.org/10.1007/s00497-021-00433-0","url":null,"abstract":"The pollen tube is ideal for studying cell polar growth, and observing the ultrastructure of the pollen tube tip using transmission electron microscopy (TEM) is the primary method for studying pollen tube growth. The preparation of ultrathin sections of the pollen tube tip sample is important for its successful microscopic observation. The direction of pollen tube growth in vitro is irregular, and it is difficult to dissect the tip of the pollen tube during ultrathin sectioning. Here, we used two methods to efficiently obtain an ultrathin section of the pollen tube tip of Pyrus. In the first method, laser micro-cutting was used to obtain the pollen tube tip, followed by ultrathin sectioning. In the other method, the pollen tubes were cultured in the same growth direction, followed by ultrathin sectioning. Ultrathin sections, which were observed via TEM, showed typical characteristics of the pollen tube tip, such as dense vesicles, numerous mitochondria, and secretory vesicles of the Golgi. We concluded that these two methods are effective in pollen tube tip sample preparation for ultrathin sectioning and provide the foundation for observing the ultrastructure of pollen tube tips.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39853530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRM61 is essential for Arabidopsis embryo and endosperm development. TRM61对拟南芥胚和胚乳发育至关重要。

IF 3.4 4区生物学

Plant Reproduction Pub Date : 2022-03-01 Epub Date: 2021-08-18 DOI: 10.1007/s00497-021-00428-x

Mohammad Aslam, Xiaoyi Huang, Maokai Yan, Zeyuan She, Xiangyu Lu, Beenish Fakher, Yingzhi Chen, Gang Li, Yuan Qin

{"title":"TRM61 is essential for Arabidopsis embryo and endosperm development.","authors":"Mohammad Aslam, Xiaoyi Huang, Maokai Yan, Zeyuan She, Xiangyu Lu, Beenish Fakher, Yingzhi Chen, Gang Li, Yuan Qin","doi":"10.1007/s00497-021-00428-x","DOIUrl":"https://doi.org/10.1007/s00497-021-00428-x","url":null,"abstract":"Post-transcriptional modifications of tRNA molecules play crucial roles in gene expression and protein biosynthesis. Across the genera, methylation of tRNAs at N1 of adenosine 58 (A58) by AtTRM61/AtTRM6 complex plays a critical role in maintaining the stability of initiator methionyl-tRNA (tRNAiMet). Recently, it was shown that mutation in AtTRM61 or AtTRM6 leads to seed abortion. However, a detailed study about the AtTRM61/AtTRM6 function in plants remains vague. Here, we found that AtTRM61 has a conserved functional structure and possesses conserved binding motifs for cofactor S-adenosyl-L-methionine (AdoMet). Mutations of the complex subunits AtTRM61/AtTRM6 result in embryo and endosperm developmental defects. The endosperm and embryo developmental defects were conditionally complemented by Attrm61-1/ + FIS2pro::AtTRM61 and Attrm61-1/ + ABI3pro::AtTRM61 indicating that AtTRM61 is required for early embryo and endosperm development. Besides, the rescue of the fertility defects in trm61/ + by overexpression of initiator tRNA suggests that AtTRM61 mutation could diminish tRNAiMet stability. Moreover, using yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays, we showed that AtMPK4 physically interacts with AtTRM61. The data presented here suggest that AtTRM61 has a conserved structure and function in Arabidopsis. Also, AtTRM61 may be required for tRNAiMet stability, embryo and endosperm development.","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00497-021-00428-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39324256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3