Repair of DNA double-strand breaks in plant meiosis: role of eukaryotic RecA recombinases and their modulators.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2023-03-01 DOI:10.1007/s00497-022-00443-6

Côme Emmenecker, Christine Mézard, Rajeev Kumar

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引用次数: 7

Abstract

Homologous recombination during meiosis is crucial for the DNA double-strand breaks (DSBs) repair that promotes the balanced segregation of homologous chromosomes and enhances genetic variation. In most eukaryotes, two recombinases RAD51 and DMC1 form nucleoprotein filaments on single-stranded DNA generated at DSB sites and play a central role in the meiotic DSB repair and genome stability. These nucleoprotein filaments perform homology search and DNA strand exchange to initiate repair using homologous template-directed sequences located elsewhere in the genome. Multiple factors can regulate the assembly, stability, and disassembly of RAD51 and DMC1 nucleoprotein filaments. In this review, we summarize the current understanding of the meiotic functions of RAD51 and DMC1 and the role of their positive and negative modulators. We discuss the current models and regulators of homology searches and strand exchange conserved during plant meiosis. Manipulation of these repair factors during plant meiosis also holds a great potential to accelerate plant breeding for crop improvements and productivity.

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植物减数分裂中DNA双链断裂的修复:真核RecA重组酶及其调节剂的作用。

减数分裂过程中的同源重组对DNA双链断裂(DSBs)修复至关重要，它促进了同源染色体的平衡分离，增强了遗传变异。在大多数真核生物中，两个重组酶RAD51和DMC1在DSB位点产生的单链DNA上形成核蛋白丝，并在减数分裂DSB修复和基因组稳定性中发挥核心作用。这些核蛋白丝进行同源性搜索和DNA链交换，利用基因组中其他位置的同源模板导向序列启动修复。RAD51和DMC1核蛋白细丝的组装、稳定和拆卸受多种因素的调控。本文就RAD51和DMC1的减数分裂功能及其正、负调节因子的作用作一综述。我们讨论了植物减数分裂过程中同源性搜索和链交换的现有模式和调控因子。在植物减数分裂过程中对这些修复因子的操纵也有很大的潜力来加速植物育种，以改善作物和提高生产力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464