DNA Research最新文献

Chromosome-scale genome assembly of Phyllanthus emblica L. "Yingyu".

IF 3.9 2区生物学

DNA Research Pub Date : 2025-03-12 DOI: 10.1093/dnares/dsaf006

Lumin Zhang, Jianmin Yuan, Tianlei Pu, Wenlin Qv, Xiao Lei, Kaihua Ma, Kunjian Qian, Qiongling Zhao, Chengfei Liao, Jie Jin

{"title":"Chromosome-scale genome assembly of Phyllanthus emblica L. \"Yingyu\".","authors":"Lumin Zhang, Jianmin Yuan, Tianlei Pu, Wenlin Qv, Xiao Lei, Kaihua Ma, Kunjian Qian, Qiongling Zhao, Chengfei Liao, Jie Jin","doi":"10.1093/dnares/dsaf006","DOIUrl":"https://doi.org/10.1093/dnares/dsaf006","url":null,"abstract":"Phyllanthus emblica L. is an edible plant with medicinal properties native to the dry-hot valley of Yunnan, China. Here, we report a de novo chromosome-scale genome of P. emblica wild type \"Yingyu\". \"Yingyu\" is an octopoid plant with a total of 104 chromosomes. In total, we assembled and clustered 480 Mb of the genome and constructed 26 pseudochromosomes (haplotypes) of P. emblica wild type \"Yingyu\" that encompass 97.9% of the genome and demonstrate to have relatively high integrity. We annotated 31,111 genes found in the genome of P. emblica. We screened five different tissues for searching the tissue-specific expression candidate genes. Four unknown function candidate genes were expressed at high levels in the flowers while genes relating to the biosynthesis of gibberellins and cellulose were specifically expressed in the fruits. The ascorbate biosynthesis related genes were screened on P. emblica \"Yingyu\" genome. The high expression level of two GDP-mannose epimerases and one L-galactono-1,4- lactone dehydrogenases in the fruit may be related to the active of absorbate biosynthesis in the fruit. The chromosome-level genomic data for P. emblica we report will be important for the development of molecular markers to facilitate the selection of superior cultivars for the processing and pharmaceuticals.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome-level genome assembly of Pontederia cordata L. provides insights into its rapid adaptation and variation of flower colours.

IF 3.9 2区生物学

DNA Research Pub Date : 2025-03-01 DOI: 10.1093/dnares/dsaf002

Jia-Le Wang, Wen-Da Zhang, Xiao-Dong Yang, Pu-Guang Zhao, Xiang-Yu Wang, Shu-Ying Zhao, Ling-Yun Chen

{"title":"Chromosome-level genome assembly of Pontederia cordata L. provides insights into its rapid adaptation and variation of flower colours.","authors":"Jia-Le Wang, Wen-Da Zhang, Xiao-Dong Yang, Pu-Guang Zhao, Xiang-Yu Wang, Shu-Ying Zhao, Ling-Yun Chen","doi":"10.1093/dnares/dsaf002","DOIUrl":"10.1093/dnares/dsaf002","url":null,"abstract":"Pontederia cordata L. is an aquatic ornamental plant native to the Americas but has been widely distributed in South Asia, Australia, and Europe. The genetic mechanisms behind its rapid adaptation and spread have not yet been well understood. To understand the mechanisms for its rapid adaptation, this study assembled the first chromosome-level genome of P. cordata. The genome assembly, which spans 527.5 Mb, is anchored on 8 pseudochromosomes with a scaffold N50 of 48 Mb and encompasses 29,389 protein-coding genes. Further analyses revealed that P. cordata had experienced 3 whole-genome duplications (WGDs) events. These WGDs are associated with gene family expansion and increased numbers of resistance gene analogs and transcription factors. Positive selection analysis indicated that genes derived from tandem duplication (TD) and proximal duplication were more likely to undergo positive selection, and were enriched in plant defense and disease resistance. These results implied that WGDs, TD, and positive selection enhanced the environmental adaptability of P. cordata. In addition, we found that down-regulation of F3'5'H, DFR, ANS, and UFGT likely caused the flower colour variation for P. cordata from violet to white. The first chromosome-level genome of P. cordata here provides a valuable genomic resource for investigating the rapid adaptation and flower colour variation of the species.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143061071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Assembly of the temporary socially parasitic spiny ant Polyrhachis lamellidens and its host Camponotus japonicus.

IF 3.9 2区生物学

DNA Research Pub Date : 2025-03-01 DOI: 10.1093/dnares/dsaf005

Hironori Iwai, Yu Kurihara, Nobuaki Kono, Masaru K Hojo, Katsushi Yamaguchi, Shuji Shigenobu, Mamiko Ozaki, Akiko Koto, Kazuharu Arakawa

{"title":"Genome Assembly of the temporary socially parasitic spiny ant Polyrhachis lamellidens and its host Camponotus japonicus.","authors":"Hironori Iwai, Yu Kurihara, Nobuaki Kono, Masaru K Hojo, Katsushi Yamaguchi, Shuji Shigenobu, Mamiko Ozaki, Akiko Koto, Kazuharu Arakawa","doi":"10.1093/dnares/dsaf005","DOIUrl":"https://doi.org/10.1093/dnares/dsaf005","url":null,"abstract":"Polyrhachis lamellidens is a temporary socially parasitic ant. The newly mated P. lamellidens queen takes over a colony of several Camponotus ant species and uses the labor of the host workers in the early stages of social parasitism. To facilitate genomic resources for these species, we assembled and annotated the chromosomal genome of P. lamellidens using the 10× Genomics linked-read and Hi-C sequencing, and the draft genome of its host, Camponotus japonicus, using long-read sequencing with the Revio system. The P. lamellidens chromosomal genome assembly is 214.1 Mb, 95.5% BUSCO completeness, and contains 13,703 protein-coding genes. The C. japonicus draft genome assembly is 314.2 Mb, 99.0% BUSCO completeness, and contains 11,207 protein-coding genes. Genome-wide phylogeny and synteny analysis confirmed the phylogenetic position of P. lamellidens and C. japonicus, and a high level of synteny with the genome of both ant species. In addition, P. lamellidens possesses nearly identical chemosensory proteins to its host, C. japonicus, and these genes tended to exhibit higher expression levels in the newly mated queen. The genome assemblies of P. lamellidens and its host C. japonicus provide a valuable resource for the molecular biological and bioinformatic basis for studying the strategy of social parasitism in ants.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative transcriptome and mutation analyses of the pancreatic islets of a rat model of obese type 2 diabetes identifies a frequently distributed nonsense mutation in the lipocalin 2 gene.

IF 3.9 2区生物学

DNA Research Pub Date : 2025-02-26 DOI: 10.1093/dnares/dsaf004

Norihide Yokoi, Naoki Adachi, Tomoki Hirokoji, Kenta Nakano, Minako Yoshihara, Saki Shigenaka, Ryuya Urakawa, Yukio Taniguchi, Yusaku Yoshida, Shigeo Yokose, Mikita Suyama, Tadashi Okamura

{"title":"Comparative transcriptome and mutation analyses of the pancreatic islets of a rat model of obese type 2 diabetes identifies a frequently distributed nonsense mutation in the lipocalin 2 gene.","authors":"Norihide Yokoi, Naoki Adachi, Tomoki Hirokoji, Kenta Nakano, Minako Yoshihara, Saki Shigenaka, Ryuya Urakawa, Yukio Taniguchi, Yusaku Yoshida, Shigeo Yokose, Mikita Suyama, Tadashi Okamura","doi":"10.1093/dnares/dsaf004","DOIUrl":"https://doi.org/10.1093/dnares/dsaf004","url":null,"abstract":"Type 2 diabetes is a multifactorial disease caused by insulin resistance and impaired insulin secretion from pancreatic β-cells, but the precise mechanisms remain to be elucidated. To identify primary genetic factors of type 2 diabetes in a rat model, we performed comparative transcriptome and mutation analyses of the pancreatic islets between the obese Zucker fatty rat and the Zucker fatty rat-derived type 2 diabetes model ZFDM rat. Among differentially expressed genes irrespective of obesity and glucose intolerance states, we identified a nonsense mutation, c.409C>T (p.Gln137X), in the lipocalin 2 (Lcn2) gene which encodes a secreted protein called neutrophil gelatinase-associated lipocalin, a well-known biomarker for inflammation. We examined the relevance of the Lcn2 mutation with T2D in the ZFDM rat by using genome editing and genetic linkage analysis and confirmed that the Lcn2 mutation exhibits no significant association with the onset of T2D. Interestingly, we found that the Lcn2 mutation is distributed widely in rat species, such as commonly used DA and F344 strains. Our data indicate that several rat strains would serve as Lcn2 deficient models, contributing to elucidate pathophysiological roles of Lcn2 in a wide variety of phenotypes.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The haplotype-phased genome assembly facilitated the deciphering of the bud dormancy-related QTLs in Prunus mume. 单倍型阶段基因组组装促进了李芽休眠相关qtl的破译。

IF 3.9 2区生物学

DNA Research Pub Date : 2024-12-27 DOI: 10.1093/dnares/dsae034

Tzu-Fan Hsiang, Hisayo Yamane, Yuan-Jui Lin, Miku Sugimori, Soichiro Nishiyama, Kyoka Nagasaka, Ryohei Nakano, Ryutaro Tao

{"title":"The haplotype-phased genome assembly facilitated the deciphering of the bud dormancy-related QTLs in Prunus mume.","authors":"Tzu-Fan Hsiang, Hisayo Yamane, Yuan-Jui Lin, Miku Sugimori, Soichiro Nishiyama, Kyoka Nagasaka, Ryohei Nakano, Ryutaro Tao","doi":"10.1093/dnares/dsae034","DOIUrl":"10.1093/dnares/dsae034","url":null,"abstract":"Bud dormancy is a vital physiological process in woody perennials, facilitating their adaptation to seasonal environmental changes. Satisfying genotype-specific chilling requirements (CR) and heat requirements (HR) through exposure to specific chilling and warm temperatures is essential for dormancy release and the subsequent resumption of growth. The genetic mechanisms regulating bud dormancy traits in Prunus mume remain unclear. In this study, we first assembled the genome of 'Nanko', the leading P. mume cultivar in Japan, in a haplotype-resolved manner. Using an F1 segregating population from a cross between 'Nanko' (high-chill) and 'SC' (low-chill), a cultivar adapted to subtropical conditions, we identified quantitative trait loci (QTLs) for vegetative bud dormancy traits on chromosome 4 (LG4 QTLs) in the 'Nanko' genome and for CR and HR on chromosome 7 (LG7 QTL) in the 'SC' genome. A notable 5.6 Mb chromosome inversion was overlapped with LG4 QTL interval in one of the 'Nanko' haplotypes. We also identified candidate genes based on haplotyping, differential expression between the parents or the presence of trait-correlated variants in coding regions. Notably, genes such as PmuMAIN, PmuNAC2, PmuDOG1, PmuSUI1, PmuATG8CL, PmubZIP44, and PmuSAUR50 were identified. This study provides valuable insights into the genetic regulation of vegetative bud dormancy in Prunus species.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11747360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-resolved analysis of Serratia marcescens strain SMTT infers niche specialization as a hydrocarbon-degrader. 粘质沙雷氏菌SMTT的基因组解析分析推断其作为烃类降解物的生态位特化。

IF 3.9 2区生物学

DNA Research Pub Date : 2024-12-27 DOI: 10.1093/dnares/dsaf001

Amanda C Ramdass, Sephra N Rampersad

{"title":"Genome-resolved analysis of Serratia marcescens strain SMTT infers niche specialization as a hydrocarbon-degrader.","authors":"Amanda C Ramdass, Sephra N Rampersad","doi":"10.1093/dnares/dsaf001","DOIUrl":"10.1093/dnares/dsaf001","url":null,"abstract":"Bacteria that are chronically exposed to high levels of pollutants demonstrate genomic and corresponding metabolic diversity that complement their strategies for adaptation to hydrocarbon-rich environments. Whole genome sequencing was carried out to infer functional traits of Serratia marcescens strain SMTT recovered from soil contaminated with crude oil. The genome size (Mb) was 5,013,981 with a total gene count of 4,842. Comparative analyses with carefully selected S. marcescens strains, 2 of which are associated with contaminated soil, show conservation of central metabolic pathways in addition to intra-specific genetic diversity and metabolic flexibility. Genome comparisons also indicated an enrichment of genes associated with multidrug resistance and efflux pumps for SMTT. The SMTT genome contained genes that enable the catabolism of aromatic compounds via the protocatechuate para-degradation pathway, in addition to meta-cleavage of catechol (meta-cleavage pathway II); gene enrichment for aromatic compound degradation was markedly higher for SMTT compared to the other S. marcescens strains analysed. Our data presents a valuable genetic inventory for future studies on strains of S. marcescens and provides insights into those genomic features of SMTT with industrial potential.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Chromosome-level genome assembly of the medicinal insect Blaps rhynchopetera using Nanopore and Hi-C technologies. 修正：利用纳米孔和Hi-C技术对药用昆虫舌翅的染色体水平基因组组装。

IF 3.9 2区生物学

DNA Research Pub Date : 2024-12-27 DOI: 10.1093/dnares/dsae037

引用次数: 0

A fully phased, chromosome-scale genome of sugar beet line FC309 enables the discovery of Fusarium yellows resistance QTL. 甜菜品系 FC309 的全分期染色体级基因组有助于发现抗黄萎病镰刀菌的 QTL。

IF 3.9 2区生物学

DNA Research Pub Date : 2024-12-27 DOI: 10.1093/dnares/dsae032

Olivia E Todd, Sheron Simpson, Brian Scheffler, Kevin M Dorn

{"title":"A fully phased, chromosome-scale genome of sugar beet line FC309 enables the discovery of Fusarium yellows resistance QTL.","authors":"Olivia E Todd, Sheron Simpson, Brian Scheffler, Kevin M Dorn","doi":"10.1093/dnares/dsae032","DOIUrl":"10.1093/dnares/dsae032","url":null,"abstract":"Sugar beet (Beta vulgaris L.) is a global source of table sugar and animal fodder. Here we report a highly contiguous, haplotype phased genome assembly and annotation for sugar beet line FC309. Both assembled haplomes for FC309 represent the largest and most contiguous assembled beet genomes reported to date, as well as gene annotations sets that capture over 1,500 additional protein-coding loci compared to prior beet genome annotations. These new genomic resources were used to identify novel quantitative trait loci (QTL) for Fusarium yellows resistance from the FC309 genetic background using an F2 mapping-by-sequencing approach. The highest QTL signals were detected on Chromosome 3, spanning approximately 10Mbp in both haplomes. A parallel transcriptome profiling experiment identified candidate genes within the Chromosome 3 QTL with plausible roles in disease response, including NBS-LRR genes with expression trends supporting a role in resistance. Investigation of genetic variants in these candidate genes found 1 major disease-resistance protein containing high-effect variants of interest. Collectively, the genomic resources for FC309 presented here are foundational tools for comparative genomics, mapping other traits in the FC309 background, and as a reference genome for other beet studies due to its contiguity, completeness, and high-quality gene annotations.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11747354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Near-complete telomere-to-telomere de novo genome assembly in Egyptian clover (Trifolium alexandrinum). 埃及三叶草（Trifolium alexandrinum）近乎完整的端粒到端粒从头基因组组装。

IF 3.9 2区生物学

DNA Research Pub Date : 2024-12-27 DOI: 10.1093/dnares/dsae036

Mitsuhiko P Sato, Ramadan A Arafa, Mohamed T Rakha, Amero A Emeran, Sachiko Isobe, Kenta Shirasawa

{"title":"Near-complete telomere-to-telomere de novo genome assembly in Egyptian clover (Trifolium alexandrinum).","authors":"Mitsuhiko P Sato, Ramadan A Arafa, Mohamed T Rakha, Amero A Emeran, Sachiko Isobe, Kenta Shirasawa","doi":"10.1093/dnares/dsae036","DOIUrl":"10.1093/dnares/dsae036","url":null,"abstract":"Egyptian clover (Trifolium alexandrinum L.), also known as berseem clover, is an important forage crop to semi-arid conditions that was domesticated in ancient Egypt in 5,5000 BCE and introduced and well adapted to numerous countries including India, Pakistan, Turkey, and Mediterranean region. Despite its agricultural importance, genomic research on Egyptian clover has been limited to developing efficient modern breeding programs. In the present study, we constructed near-complete telomere-to-telomere-level genome assemblies for 2 Egyptian clover cultivars, Helaly and Fahl. Initial assemblies were established by using highly fidelity long-read technology. To extend sequence contiguity, we developed a gap-targeted sequencing (GAP-Seq) method, in which contig ends are targeted for sequencing to obtain long reads bridging 2 contigs. The total length of the resultant chromosome-level assemblies was 547.7 Mb for Helaly and 536.3 Mb for Fahl. These differences in sequence length can be attributed to the expansion of DNA transposons. Population genomic analysis using single-nucleotide polymorphisms revealed genomic regions highly differentiated between 2 cultivars and increased genetic uniformity within each cultivar. Gene ontologies associated with metabolic and biosynthetic processes and developmental processes were enriched in these genomic regions, indicating that these genes may determine the unique characteristics of each cultivar. Comprehensive genomic resources can provide valuable insights into genetic improvements in Egyptian clover and legume genomics.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11747361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a candidate sex determination region and sex-specific molecular markers based on whole-genome re‑sequencing in the sea star Asterias amurensis. 基于全基因组重测序的海星候选性别决定区和性别特异性分子标记的鉴定。

IF 3.9 2区生物学

DNA Research Pub Date : 2024-12-27 DOI: 10.1093/dnares/dsaf003

Yanlin Wang, Hongliang Yang, Yixin Wang, Yulong Li, Gang Ni, Scott F Cummins, Muyan Chen

{"title":"Identification of a candidate sex determination region and sex-specific molecular markers based on whole-genome re‑sequencing in the sea star Asterias amurensis.","authors":"Yanlin Wang, Hongliang Yang, Yixin Wang, Yulong Li, Gang Ni, Scott F Cummins, Muyan Chen","doi":"10.1093/dnares/dsaf003","DOIUrl":"10.1093/dnares/dsaf003","url":null,"abstract":"Sex determination systems are diverse in echinoderms, however, our understanding is still very limited in this research field, especially for Asteroidea species. The northern Pacific seastar, Asterias amurensis, has attracted widespread concern due to its population outbreaks and high-risk invasions. Using whole-genome re-sequencing data from 40 females and 40 males, we identified a candidate sex determination region in A. amurensis. Based on the distribution characteristics of 525 sex-associated single nucleotide polymorphisms, identified by GWAS analysis, 119 sex-specific loci were isolated combining a custom Perl script, PCA analysis, and the selection signatures of fixation index FST, suggesting that a 7-12 Mb region on chromosome 10 is a candidate sex-determining region. The existence of female-specific sequences and the genotypes of sex-specific loci indicated that A. amurensis might utilize a ZZ/ZW sex-determination system. We also developed two pairs of sex-specific primers that could distinguish the genetic sex of this starfish with 100% accuracy. As the first study on sex determination in Asteroidea, it will provide novel insights into diverse sex determination systems in echinoderms and allow for in-depth studies on sex-related eco-physiological issues in A. amurensis.","PeriodicalId":51014,"journal":{"name":"DNA Research","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0