Advances in Virus Research最新文献_第6页

Applications of mass spectrometry imaging in virus research. 质谱成像在病毒研究中的应用。

2区医学

Advances in Virus Research Pub Date : 2021-01-01 Epub Date: 2020-11-09 DOI: 10.1016/bs.aivir.2020.10.002

Luca D Bertzbach, Benedikt B Kaufer, Axel Karger

{"title":"Applications of mass spectrometry imaging in virus research.","authors":"Luca D Bertzbach, Benedikt B Kaufer, Axel Karger","doi":"10.1016/bs.aivir.2020.10.002","DOIUrl":"https://doi.org/10.1016/bs.aivir.2020.10.002","url":null,"abstract":"Mass spectrometry imaging (MSI) is a label-free molecular imaging technique allowing an untargeted detection of a broad range of biomolecules and xenobiotics. MSI enables imaging of the spatial distribution of proteins, peptides, lipids and metabolites from a wide range of samples. To date, this technique is commonly applied to tissue sections in cancer diagnostics and biomarker development, but also molecular histology in general. Advances in the methodology and bioinformatics improved the resolution of MS images below the single cell level and increased the flexibility of the workflow. However, MSI-based research in virology is just starting to gain momentum and its full potential has not been exploited yet. In this review, we discuss the main applications of MSI in virology. We review important aspects of matrix-assisted laser desorption/ionization (MALDI) MSI, the most widely used MSI technique in virology. In addition, we summarize relevant literature on MSI studies that aim to unravel virus-host interactions and virus pathogenesis, to elucidate antiviral drug kinetics and to improve current viral disease diagnostics. Collectively, these studies strongly improve our general understanding of virus-induced changes in the proteome, metabolome and metabolite distribution in host tissues of humans, animals and plants upon infection. Furthermore, latest MSI research provided important insights into the drug distribution and distribution kinetics, especially in antiretroviral research. Finally, MSI-based investigations of oncogenic viruses greatly increased our knowledge on tumor mass signatures and facilitated the identification of cancer biomarkers.","PeriodicalId":50977,"journal":{"name":"Advances in Virus Research","volume":"109 ","pages":"31-62"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/bs.aivir.2020.10.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38938845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

A great ape perspective on the origins and evolution of human viruses. 从类人猿的角度看人类病毒的起源和进化。

2区医学

Advances in Virus Research Pub Date : 2021-01-01 Epub Date: 2021-07-08 DOI: 10.1016/bs.aivir.2021.06.001

Sébastien Calvignac-Spencer, Ariane Düx, Jan F Gogarten, Fabian H Leendertz, Livia V Patrono

引用次数: 7

Advancing phage therapy through the lens of virus host-breadth and emergence potential. 从病毒宿主宽度和出现潜力的角度推进噬菌体治疗。

2区医学

Advances in Virus Research Pub Date : 2021-01-01 Epub Date: 2021-09-02 DOI: 10.1016/bs.aivir.2021.07.004

Lisa M Bono, Stephanie Mao, Rachel E Done, Kenichi W Okamoto, Benjamin K Chan, Paul E Turner

{"title":"Advancing phage therapy through the lens of virus host-breadth and emergence potential.","authors":"Lisa M Bono, Stephanie Mao, Rachel E Done, Kenichi W Okamoto, Benjamin K Chan, Paul E Turner","doi":"10.1016/bs.aivir.2021.07.004","DOIUrl":"https://doi.org/10.1016/bs.aivir.2021.07.004","url":null,"abstract":"Phages are viruses that specifically infect bacteria, and their biodiversity contributes to historical and current development of phage therapy to treat myriad bacterial infections. Phage therapy holds promise as an alternative to failing chemical antibiotics, but there are benefits and costs of this technology. Here, we review the rich history of phage therapy, highlighting reasons (often political) why it was widely rejected by Western medicine until recently. One longstanding idea involves mixing different phages together in cocktails, to increase the probability of killing target pathogenic bacteria without pre-screening for phage susceptibility. By challenging 30 lytic phages to infect 14 strains of the bacteria Pseudomonas aeruginosa, we showed that some phages were \"generalists\" with broad host-ranges, emphasizing that extreme host-specificity of phages was not necessarily a liability. Using a \"greedy algorithm\" analysis, we identified the best cocktail mixture of phages to achieve broad bacteria killing. Additionally, we review how virus host-range can evolve and connect lessons learned from virus emergence-including contributions of elevated virus mutation rates in promoting emergence and virus evolutionary transitions from specialized to generalized host-use-as cautionary tales for avoiding risk of \"off-target\" phage emergence on commensal bacteria in microbiomes. Throughout, we highlight how fundamental understanding of virus ecology and evolution is vital for developing phage therapy; heeding these principles should help in designing therapeutic strategies that do not recapitulate consequences of virus selection to emerge on novel hosts.","PeriodicalId":50977,"journal":{"name":"Advances in Virus Research","volume":" ","pages":"63-110"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39532041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A holistic perspective on herpes simplex virus (HSV) ecology and evolution. 单纯疱疹病毒(HSV)生态学和进化的整体观点。

2区医学

Advances in Virus Research Pub Date : 2021-01-01 Epub Date: 2021-06-26 DOI: 10.1016/bs.aivir.2021.05.001

Molly M Rathbun, Moriah L Szpara

{"title":"A holistic perspective on herpes simplex virus (HSV) ecology and evolution.","authors":"Molly M Rathbun, Moriah L Szpara","doi":"10.1016/bs.aivir.2021.05.001","DOIUrl":"https://doi.org/10.1016/bs.aivir.2021.05.001","url":null,"abstract":"Herpes simplex viruses (HSV) cause chronic infection in humans that are characterized by periodic episodes of mucosal shedding and ulcerative disease. HSV causes millions of infections world-wide, with lifelong bouts of viral reactivation from latency in neuronal ganglia. Infected individuals experience different levels of disease severity and frequency of reactivation. There are two distantly related HSV species, with HSV-1 infections historically found most often in the oral niche and HSV-2 infections in the genital niche. Over the last two decades, HSV-1 has emerged as the leading cause of first-episode genital herpes in multiple countries. While HSV-1 has the highest level of genetic diversity among human alpha-herpesviruses, it is not yet known how quickly the HSV-1 viral population in a human host adapts over time, or if there are population bottlenecks associated with viral reactivation and/or transmission. It is also unknown how the ecological environments in which HSV infections occur influence their evolutionary trajectory, or that of co-occurring viruses and microbes. In this review, we explore how HSV accrues genetic diversity within each new infection, and yet maintains its ability to successfully infect most of the human population. A holistic examination of the ecological context of natural human infections can expand our awareness of how HSV adapts as it moves within and between human hosts, and reveal the complexity of these lifelong human-virus interactions. These insights may in turn suggest new areas of exploration for other chronic pathogens that successfully evolve and persist among their hosts.","PeriodicalId":50977,"journal":{"name":"Advances in Virus Research","volume":" ","pages":"27-57"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/bs.aivir.2021.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39288392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Parainfluenza virus entry at the onset of infection. 副流感病毒在感染开始时进入。

2区医学

Advances in Virus Research Pub Date : 2021-01-01 DOI: 10.1016/bs.aivir.2021.07.001

Tara C Marcink, Matteo Porotto, Anne Moscona

{"title":"Parainfluenza virus entry at the onset of infection.","authors":"Tara C Marcink, Matteo Porotto, Anne Moscona","doi":"10.1016/bs.aivir.2021.07.001","DOIUrl":"https://doi.org/10.1016/bs.aivir.2021.07.001","url":null,"abstract":"Parainfluenza viruses, members of the enveloped, negative-sense, single stranded RNA Paramyxoviridae family, impact global child health as the cause of significant lower respiratory tract infections. Parainfluenza viruses enter cells by fusing directly at the cell surface membrane. How this fusion occurs via the coordinated efforts of the two molecules that comprise the viral surface fusion complex, and how these efforts may be blocked, are the subjects of this chapter. The receptor binding protein of parainfluenza forms a complex with the fusion protein of the virus, remaining stably associated until a receptor is reached. At that point, the receptor binding protein actively triggers the fusion protein to undergo a series of transitions that ultimately lead to membrane fusion and viral entry. In recent years it has become possible to examine this remarkable process on the surface of viral particles and to begin to understand the steps in the transition of this molecular machine, using a structural biology approach. Understanding the steps in entry leads to several possible strategies to prevent fusion and inhibit infection.","PeriodicalId":50977,"journal":{"name":"Advances in Virus Research","volume":"111 ","pages":"1-29"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10270304/pdf/nihms-1903208.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9633911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Preface. 前言。

2区医学

Advances in Virus Research Pub Date : 2021-01-01 DOI: 10.1016/S0065-3527(21)00014-2

Gisa Gerold

引用次数: 0

Alphavirus RNA replication in vertebrate cells. 脊椎动物细胞中的甲病毒RNA复制。

2区医学

Advances in Virus Research Pub Date : 2021-01-01 Epub Date: 2021-08-06 DOI: 10.1016/bs.aivir.2021.07.003

Tero Ahola, Gerald McInerney, Andres Merits

引用次数: 15

Molecular archeology of human viruses. 人类病毒的分子考古学。

2区医学

Advances in Virus Research Pub Date : 2021-01-01 Epub Date: 2021-08-11 DOI: 10.1016/bs.aivir.2021.07.002

Sébastien Calvignac-Spencer, Ariane Düx, Jan F Gogarten, Livia V Patrono

引用次数: 2

Virus systems biology: Proteomics profiling of dynamic protein networks during infection. 病毒系统生物学:感染过程中动态蛋白质网络的蛋白质组学分析。

2区医学

Advances in Virus Research Pub Date : 2021-01-01 Epub Date: 2021-01-04 DOI: 10.1016/bs.aivir.2020.12.001

Kevin Klann, Georg Tascher, Christian Münch

引用次数: 3

Proximity labeling approaches to study protein complexes during virus infection. 接近标记方法研究病毒感染过程中的蛋白质复合物。

2区医学

Advances in Virus Research Pub Date : 2021-01-01 Epub Date: 2021-04-16 DOI: 10.1016/bs.aivir.2021.02.001

Francisco José Zapatero-Belinchón, Belén Carriquí-Madroñal, Gisa Gerold

{"title":"Proximity labeling approaches to study protein complexes during virus infection.","authors":"Francisco José Zapatero-Belinchón, Belén Carriquí-Madroñal, Gisa Gerold","doi":"10.1016/bs.aivir.2021.02.001","DOIUrl":"https://doi.org/10.1016/bs.aivir.2021.02.001","url":null,"abstract":"Cellular compartmentalization of proteins and protein complex formation allow cells to tightly control biological processes. Therefore, understanding the subcellular localization and interactions of a specific protein is crucial to uncover its biological function. The advent of proximity labeling (PL) has reshaped cellular proteomics in infection biology. PL utilizes a genetically modified enzyme that generates a \"labeling cloud\" by covalently labeling proteins in close proximity to the enzyme. Fusion of a PL enzyme to a specific antibody or a \"bait\" protein of interest in combination with affinity enrichment mass spectrometry (AE-MS) enables the isolation and identification of the cellular proximity proteome, or proxisome. This powerful methodology has been paramount for the mapping of membrane or membraneless organelles as well as for the understanding of hard-to-purify protein complexes, such as those of transmembrane proteins. Unsurprisingly, more and more infection biology research groups have recognized the potential of PL for the identification of host-pathogen interactions. In this chapter, we introduce the enzymes commonly used for PL labeling as well as recent promising advancements and summarize the major achievements in organelle mapping and nucleic acid PL. Moreover, we comprehensively describe the research on host-pathogen interactions using PL, giving special attention to studies in the field of virology.","PeriodicalId":50977,"journal":{"name":"Advances in Virus Research","volume":"109 ","pages":"63-104"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/bs.aivir.2021.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38938846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1