Pharmacia最新文献_第6页

HPLC method for the determination of nifedipine in rat plasma: development, validation, and application to pharmacokinetic drug-herb interaction study 测定大鼠血浆中硝苯地平含量的高效液相色谱法的建立、验证及在药物-草药相互作用研究中的应用

Pharmacia Pub Date : 2024-02-26 DOI: 10.3897/pharmacia.71.e119198

Devy N. A. Hasanuddin, Afrillia Nuryanti Garmana, Lucy Sasongko

引用次数: 0

Gallates – toxicological data and a pilot study on the prevalence of contact sensitization 没食子酸盐--毒理学数据和接触致敏试验研究

Pharmacia Pub Date : 2024-02-23 DOI: 10.3897/pharmacia.71.e120507

M. Lyapina, Silvia P. Tsanova-Savova, M. Nikolova, M. Vizeva, Georgi Momekov

引用次数: 0

Fenugreek galactomannan: High-Performance Thin-Layer Chromatography (HPTLC) method for identification and quantification of galactose and mannose 葫芦巴半乳甘露聚糖：鉴定和定量半乳糖和甘露糖的高效薄层色谱法 (HPTLC)

Pharmacia Pub Date : 2024-02-23 DOI: 10.3897/pharmacia.71.e118029

Vanya Nalbantova, Velislava Todorova, N. Benbassat, Cedric Delattre

{"title":"Fenugreek galactomannan: High-Performance Thin-Layer Chromatography (HPTLC) method for identification and quantification of galactose and mannose","authors":"Vanya Nalbantova, Velislava Todorova, N. Benbassat, Cedric Delattre","doi":"10.3897/pharmacia.71.e118029","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e118029","url":null,"abstract":"Fenugreek (Trigonella foenum-graecum L.) is a leguminous, medicinal plant that finds applications in traditional medicine and as an agent in pharmaceutical and nutraceutical products. Its seeds contain various compounds to which numerous beneficial effects are attributed, such as soluble polysaccharides, in particular galactomannan. There are limited data reporting the determination of Bulgarian fenugreek galactomannan composition by HPTLC. The analysis was successful in separating galactose and mannose, each demonstrating different Rf values 0.36, 0.48, respectively. After developing the method with the appropriate conditions, it was validated for linearity, accuracy and precision, range, limits of detection (LD) and quantification (LQ), and robustness. The LD of galactose and mannose was found at 91.34 ng/band and 85.26 ng/band, respectively. The method confirmed the composition of hydrolyzed galactomannan from fenugreek, revealing a ratio of galactose to mannose closed to 1:1, and this capability can be of significant value in both the pharmaceutical and food industries.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140436514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of newer classes of antidiabetics and the concept of pharmaceutical care – dealing with therapeutic problems 新型抗糖尿病药的比较分析和药物护理概念--处理治疗问题

Pharmacia Pub Date : 2024-02-22 DOI: 10.3897/pharmacia.71.e120141

A. Peneva, M. Dimitrov, V. Petkova

引用次数: 0

The prognostic role of markers of systemic inflammation in patients with metastatic lung cancer receiving immunotherapy: A comprehensive review of the literature 全身炎症指标在接受免疫疗法的转移性肺癌患者中的预后作用：文献综述

Pharmacia Pub Date : 2024-02-22 DOI: 10.3897/pharmacia.71.e115558

Tanya Zlatanova, J. Arabadjiev

引用次数: 0

Effects of topical Ivermectin on imiquimod-induced Psoriasis in mouse model – Novel findings 外用伊维菌素对咪喹莫特诱导的银屑病小鼠模型的影响--新发现

Pharmacia Pub Date : 2024-02-20 DOI: 10.3897/pharmacia.71.e114753

Sally Ayad Almudaris, F. Gatea

引用次数: 0

Potential of 10% propolis-based toothpaste on the inhibition of biofilm-forming bacteria growth in vitro 10%蜂胶牙膏在体外抑制生物膜形成细菌生长的潜力

Pharmacia Pub Date : 2024-02-20 DOI: 10.3897/pharmacia.71.e118072

Suryono Suryono, P. Setiawan, Nur Rahman Ahmad Seno Aji, Christia Aye Waindy Vega, Budi Rodestawati, Endang Lukitaningsih, Friska Ani Rahman, Sherlina Devina, Sholiha Sarah Tsabita, Selcaria Istna Datau, A. Alhasyimi

{"title":"Potential of 10% propolis-based toothpaste on the inhibition of biofilm-forming bacteria growth in vitro","authors":"Suryono Suryono, P. Setiawan, Nur Rahman Ahmad Seno Aji, Christia Aye Waindy Vega, Budi Rodestawati, Endang Lukitaningsih, Friska Ani Rahman, Sherlina Devina, Sholiha Sarah Tsabita, Selcaria Istna Datau, A. Alhasyimi","doi":"10.3897/pharmacia.71.e118072","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e118072","url":null,"abstract":"Aim of the study: To investigate the potential of 10% propolis-based toothpaste on inhibiting biofilm-forming bacteria growth in vitro.\u0000 Material and method: Organoleptic properties are evaluated, considering color, odor, and taste. Antibacterial tests use a disc diffusion method against Streptococcus mutans, Staphylococcus aureus and Porphyromonas gingivalis bacteria, while cytotoxicity is assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay on fibroblast cells. Statistical analysis involves mean ± standard deviation. The data were then tested using a one-way analysis of variance and Kruskal-Wallis, followed by post-hoc test (p < 0.05).\u0000 Results: The organoleptic evaluation of 10% propolis toothpaste reveals a visually clear appearance, consistent orange flavor, and aroma lasting 30 days. Based on the antibacterial results, a 10% level of propolis toothpaste sample inhibited the growth of Streptococcus mutans, Staphylococcus aureus and Porphyromonas gingivalis bacteria. The post-hoc test showed that toothpaste demonstrated significant inhibition on S. mutans and S. aureus compared to the negative control (p < 0.05). The toothpaste showed a larger inhibitory zone towards P. gingivalis compared to the adverse control; however, no significant differences were observed (p > 0.05). Cytotoxicity assessment on fibroblast cells shows a high percentage (85.31%) of viable cells. The findings highlight the 10% of propolis toothpaste’s potential and non-toxic as oral care product.\u0000 Conclusions: 10% propolis toothpaste inhibits S. mutans, S. aureus, P. gingivalis growth, and not toxic on fibroblast.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140445450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of regulatory implementation of regulation 536/2014 by European Union countries and Ukraine regarding the examination of clinical trials data and information 分析欧盟国家和乌克兰在临床试验数据和信息审查方面对第 536/2014 号法规的监管执行情况

Pharmacia Pub Date : 2024-02-20 DOI: 10.3897/pharmacia.71.e109829

L. Hala, Oleksandr Nabok

{"title":"Analysis of regulatory implementation of regulation 536/2014 by European Union countries and Ukraine regarding the examination of clinical trials data and information","authors":"L. Hala, Oleksandr Nabok","doi":"10.3897/pharmacia.71.e109829","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e109829","url":null,"abstract":"The article presents the results of a comparative analysis of regulatory requirements for expertise of clinical trials documentation, submitted for regulatory authority and ethic committees’ approval in EU member countries and Ukraine, outlining the main trends, considering the updated Regulation (EU) No 536/2014, which came into effect on January 31, 2023. Among the positive changes are simplification of safety reporting requirements, use of artificial intelligence in the process of clinical trials documentation examination for obtaining regulatory authority and ethic commission approval, introduction of a single portal for submitting materials for clinical trials, and functioning of database for the submission and review of initial Clinical Trial Application documents and obtaining authorization within the EU to facilitate the interaction between applicants and regulatory authority are highlighted. To harmonize Ukraine’s regulatory requirements with EU legislation, it is advisable to use a single portal for data exchange and document submission for applicants in regards to clinical trials, regulatory authority and local ethics committees. This will expedite the examination process of clinical trial documentation and simplify the monitoring of document review progress.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytoconstituents, antioxidant, and cholinesterase inhibitory activities of the leaves and stem extracts of Artocarpus sericicarpus 芹菜叶和茎提取物的植物成分、抗氧化性和胆碱酯酶抑制活性

Pharmacia Pub Date : 2024-02-19 DOI: 10.3897/pharmacia.71.e112499

Suciati Suciati, E. R. Laili, Hamizah Haula, L. Tumewu, N. Nuengchamnong, N. Suphrom, A. Widyawaruyanti

{"title":"Phytoconstituents, antioxidant, and cholinesterase inhibitory activities of the leaves and stem extracts of Artocarpus sericicarpus","authors":"Suciati Suciati, E. R. Laili, Hamizah Haula, L. Tumewu, N. Nuengchamnong, N. Suphrom, A. Widyawaruyanti","doi":"10.3897/pharmacia.71.e112499","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e112499","url":null,"abstract":"The study aimed to investigate the antioxidant and cholinesterase inhibitory activities of the leaves and stems of Artocarpus sericicarpus and to analyse the phenolic compounds in the extracts. The modified Ellman’s method was used to determine the cholinesterase inhibitory activities against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. The antioxidant properties were evaluated using DPPH and ABTS methods. The total phenolic content (TPC) was measured by spectrometric assay, and compound identification was carried out by LC-MS/MS analysis. The results showed that the leaf and stem extracts of A. sericicarpus exerted significant inhibitory effects against AChE and BChE, as well as antioxidant activities. The stem ethanolic extract exhibited the highest potency against AChE and BChE with IC50 values of 5.81 and 11.46 µg/mL, respectively. The leaf and stem ethanolic extracts gave higher antioxidant activities and TPC compared to the water-based extracts. The LC-MS/MS analysis indicated the presence of phenolic compounds, such as flavones, flavonols, flavanones, prenylated chalcones, and xanthones in the extracts.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140450138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adherence to disease-modifying therapies among patients with multiple sclerosis in Bulgaria – A real world study 保加利亚多发性硬化症患者坚持使用改变病情疗法--一项真实世界的研究

Pharmacia Pub Date : 2024-02-15 DOI: 10.3897/pharmacia.71.e117681

Y. Seitaridou, T. Chamova, M. Kamusheva

{"title":"Adherence to disease-modifying therapies among patients with multiple sclerosis in Bulgaria – A real world study","authors":"Y. Seitaridou, T. Chamova, M. Kamusheva","doi":"10.3897/pharmacia.71.e117681","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e117681","url":null,"abstract":"Introduction: The purpose was to assess the level of medication adherence (MA) and related factors among individuals with multiple sclerosis (MS) in Bulgaria.\u0000 Materials and methods: A prospective one-year study was conducted among 54 patients with MS diagnosed, treated, and monitored in Clinic of Neurology, University Hospital “Alexandrovska”, Sofia in 2022/2023. Clinical data, patients reported outcomes, patients’ characteristics such as age, gender and reduced work capacity, and other data were collected to define the predictors of non-adherence to medicines. MA level was assessed through a free Morisky–Green 4-item questionnaire.\u0000 Results: Мost of the observed patients were rated with high and moderate adherence to MS therapy (n = 44; 81.48%). The remaining 18.52% of the patients with poor adherence to therapy were women. There was no statistical basis for asserting differences in adherence levels among the various factors considered.\u0000 Conclusion: The current study demonstrates the importance of MA assessment and provides insights into MA among patients with MS in Bulgaria. Still, there are low-adherent patients, and the responsible factors should be further investigated.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139834508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0