Pharmacia最新文献_第5页

Effect of Equisetum ramosissimum Desf. on body weight and Leptin/Ghrelin in standard and high-fat diet Equisetum ramosissimum Desf.对标准和高脂饮食中体重和瘦素/胃泌素的影响

Pharmacia Pub Date : 2024-03-26 DOI: 10.3897/pharmacia.71.e117823

Reem A. Issa, Mahmoud Abu Samak, Manal M. Abbas, Talal Al-Qaisi, Razan Obeidat, Bayan Y. Ghanim, NA Qinna

{"title":"Effect of Equisetum ramosissimum Desf. on body weight and Leptin/Ghrelin in standard and high-fat diet","authors":"Reem A. Issa, Mahmoud Abu Samak, Manal M. Abbas, Talal Al-Qaisi, Razan Obeidat, Bayan Y. Ghanim, NA Qinna","doi":"10.3897/pharmacia.71.e117823","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e117823","url":null,"abstract":"The necessity of maintaining healthy habits, diet and minimal medical complications is challenging. The current study investigates Equisetum ramosissimum on levels of leptin, ghrelin, and their ratio in association with body weight changes in animals receiving standard and high-fat diet. Ethanolic extract was prepared by maceration and total phenols and flavonoids contents were determined using Folin-cicalto and AlCl3 methods. Study animals were fed with either standard or high-fat diets prior treatment with E. ramosissimum extract (500mg/kg). Body weights were monitored and serum concentrations of leptin, ghrelin and their ratios were determined. The plant extract comprised total phenols and flavonoids of 0.03 ± 0.01 mg/mg as Gallic acid, and 0.04 ± 0.03 mg/mg as quercetin, respectively. Findings revealed significant decrease in body weights of standard-diet animals and restoration of weight to normal after treatment with extract along with significant increase in serum ghrelin concentrations with strong correlation R and R2 values in respect to body weight. As well, significant reduction in serum leptin/ghrelin ratio was positively correlated with body weight and serum leptin levels. E. ramosissimum extract possessed promising characteristics on being introduced in dietary supplements and weight control regimens. The current study presented the influence of extract in decreasing body weight in animals fed with standard diet. Moreover, it restored the weights of obese animals which received the extract after gaining weight and being on high-fat diet. Further preclinical studies are warranted to reveal the translational potentials of E. ramosissimum extract and its vital effect on adipokines.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"114 49","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140380219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping the research on healthcare students’ empathy: Insights from a bibliometric analysis 绘制医学生移情研究图：文献计量分析的启示

Pharmacia Pub Date : 2024-03-26 DOI: 10.3897/pharmacia.71.e120826

Hening Pratiwi, S. A. Kristina, Anna Wahyuni Widayanti, Y. Prabandari

引用次数: 0

Microwave-assisted organic synthesis of pyrroles (Review) 微波辅助的吡咯有机合成（综述）

Pharmacia Pub Date : 2024-03-25 DOI: 10.3897/pharmacia.71.e119866

E. Mateev, Ali Irfan, Alexandrina Mateeva, M. Georgieva, A. Zlatkov

{"title":"Microwave-assisted organic synthesis of pyrroles (Review)","authors":"E. Mateev, Ali Irfan, Alexandrina Mateeva, M. Georgieva, A. Zlatkov","doi":"10.3897/pharmacia.71.e119866","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e119866","url":null,"abstract":"The detection of pyrrole rings in numerous organic compounds with various pharmacological activities, emphasizes its huge importance in medicinal chemistry. Thus, the synthesis of pyrroles continues to arouse interest and Paal-Knorr condensation is considered to be the main synthetic route. A significant advance has been made since the MW activation was introduced in the organic synthesis which can be confirmed with the rapid growth of the published papers on that topic. Microwave irradiation is gaining popularity since faster reaction time, higher yields, easier work-up and reduced energy input can be achieved. Furthermore, it appears in numerous green chemistry protocols. The aim of the current article was to provide insights into the microwave syntheses of pyrrole derivatives, focusing on the most used synthetic approaches - Paal-Knorr, Clauson-Kaas, Barton-Zard, Hantzsch and others. The article was divided into several sections starting with the principles of the microwave organic synthesis. Thereafter the structure and the main pharmacological effects of the pyrrole derivatives were examined. Subsequently, articles describing the synthesis of pyrroles via Paal-Knorr, Hantzsch, Clauson-Kaas and Barton– Zard were discussed. All the reviewed papers conclude a significant reduction of the reaction times after MW irradiation compared to conventional heating.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":" 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140383928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testosterone, estradiol and their ratio in male patients with acute coronary syndrome 急性冠状动脉综合征男性患者体内的睾酮、雌二醇及其比率

Pharmacia Pub Date : 2024-03-25 DOI: 10.3897/pharmacia.71.e113755

S. Shishkov, K. Hristozov, Atanas Angelov, Svetoslava Slavcheva, Yana Bocheva, M. Boyadzhieva

引用次数: 0

Oral acute toxicity study of ethanol extract of Mobe leaves (Artocarpus lacucha Buch-Ham) in Wistar rats 莫比叶（Artocarpus lacucha Buch-Ham）乙醇提取物对 Wistar 大鼠的口服急性毒性研究

Pharmacia Pub Date : 2024-03-25 DOI: 10.3897/pharmacia.71.e117500

Denny Satria, P. Sitorus, A. Dalimunthe, S. B. Waruwu, Vivi Asfianti

引用次数: 0

Pharmacists’ burnout and motivation for pharmaceutical care in chronically ill patients – a pilot study for the north-eastern region of Bulgaria 药剂师的职业倦怠与慢性病患者药物治疗的动力--保加利亚东北部地区的试点研究

Pharmacia Pub Date : 2024-03-25 DOI: 10.3897/pharmacia.71.e120566

Anna Todorova, Mariya Ivanova, V. Petkova

引用次数: 0

Antihypertensive activity of spray-dried nanoemulsion containing Asiatic acid-Palm oil in high salt diet-fed rats 含亚西亚酸-棕榈油的喷雾干燥纳米乳液对高盐饮食大鼠的抗高血压活性

Pharmacia Pub Date : 2024-03-05 DOI: 10.3897/pharmacia.71.e115091

A. Pradana, G. Ritthidej, Vudhiporn Limprasutr, Ausana Wongtayan, V. Lipipun, Iksen

{"title":"Antihypertensive activity of spray-dried nanoemulsion containing Asiatic acid-Palm oil in high salt diet-fed rats","authors":"A. Pradana, G. Ritthidej, Vudhiporn Limprasutr, Ausana Wongtayan, V. Lipipun, Iksen","doi":"10.3897/pharmacia.71.e115091","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e115091","url":null,"abstract":"Asiatic acid (AA) is a compound isolated from Centella asiatica, which possesses significant antihypertensive activity. Several studies have shown that its hypertensive activity can be attributed to various mechanisms, such as Angiotensin-Converting-Enzyme (ACE) inhibition in the renin-angiotensin-aldosterone system (RAAS) pathway. Meanwhile, palm oil (PO) is an antioxidant, which has proven to have synergistic effects with the compound by preventing arterial thrombosis and atherosclerosis. Despite these synergistic effects, AA dosage in antihypertensive therapy has been reported to be relatively high compared to the common synthetic drug captopril. Therefore, this study aimed to produce spray-dried powder of nanoemulsion to enhance the solubility of AA, decrease the possibility of oxidation, and increase its activity. Redispersed AA nanoparticles were also successfully obtained during the synthesis process. Several evaluations were carried out, including particle size, particle distribution, zeta potential, cell viability, and antihypertensive activity in rats to ensure the improvement of physicochemical characteristics and activity as antihypertensive agent. The results showed that AA succeeded in forming nanoemulsion with excipients. In addition, it was encapsulated in a maltodextrin carrier, exhibiting good physicochemical characteristics and safety to the Caco-2 cells. The redispersion of the spray-dried powder yielded nanoparticles with a size of 217.4 ± 10.196 nm. The spray-dried nanoemulsion of AA also had faster effect than non-formulated AA (raw powder) in lowering the blood pressure of hypertensive Sprague-Dawley (SD) rats.\u0000 Graphical abstract:\u0000 \u0000 \u0000","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"129 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140078761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Qualitative chemical compounds analysis and in vitro estimation of antiproliferative, antidiabetic and anti-Alzheimer’s disease effects of Ononis natrix (L.) family Fabaceae 豆科植物 Ononis natrix (L.) 的化合物定性分析及抗增殖、抗糖尿病和抗老年痴呆症作用的体外评估

Pharmacia Pub Date : 2024-03-01 DOI: 10.3897/pharmacia.71.e119755

Ahmed M M Youssef, D. A. M. Maaty, Yousef M Al-Saraireh

{"title":"Qualitative chemical compounds analysis and in vitro estimation of antiproliferative, antidiabetic and anti-Alzheimer’s disease effects of Ononis natrix (L.) family Fabaceae","authors":"Ahmed M M Youssef, D. A. M. Maaty, Yousef M Al-Saraireh","doi":"10.3897/pharmacia.71.e119755","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e119755","url":null,"abstract":"The O. natrix belongs to the family Fabaceae and is distributed in Jordan. Different species of the family Fabaceae contain chemical compounds that may have potential antiproliferative, antidiabetic, and anti-Alzheimer’s disease. High-performance liquid chromatography (HPLC) was applied to analyse the phenolic compounds found in O. natrix methanolic extract. Using the MTT assay, the antiproliferative action was studied. The enzymes α-glucosidase and butyrylcholinesterase inhibition assays were used to study the antidiabetic and anti-Alzheimer’s disease actions, respectively, of methanolic extract of O. natrix. Eleven phenolics and seven flavonoids were identified in the methanolic extract of O. natrix by HPLC. The highest phenolics and flavonoids were gallic acid (1.25 mg/100 g dry weight) and rutin (1.44 mg/100 g dry weight), respectively. The most cancer cell lines influenced by the extract of O. natrix were PC-3 (IC50 = 55 ± 2 µg/mL) and HepG-2 (IC50 = 68 ± 2 µg/mL) compared to positive control cisplatin. However, the cancer cell lines CaCo-2, MCF-7, and HeLa showed IC50 values of 109 ± 2 µg/mL, 123 ± 2 µg/mL, and 79 ± 1 µg/mL, respectively, related to cisplatin. The O. natrix extract inhibited the α-glucosidase enzyme and butyrylcholinesterase enzyme by 84% and 86%, respectively compared to positive controls acarbose and rivastigmine. The O. natrix may possess antiproliferative effects against prostate cancer and hepatocellular carcinoma. It also may have antidiabetic and anti-Alzheimer’s disease effects.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"36 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140084292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chrysin’s dose-dependent effects on steroidogenesis in female BALB/c mice: In vivo study of adrenal, ovarian, and uterine hormone regulation 蛹虫草素对雌性 BALB/c 小鼠类固醇生成的剂量依赖性影响：肾上腺、卵巢和子宫激素调节的体内研究

Pharmacia Pub Date : 2024-02-29 DOI: 10.3897/pharmacia.71.e114547

Khalid M. Alqaisi, M. Abbas, Rand Alshawawreh

{"title":"Chrysin’s dose-dependent effects on steroidogenesis in female BALB/c mice: In vivo study of adrenal, ovarian, and uterine hormone regulation","authors":"Khalid M. Alqaisi, M. Abbas, Rand Alshawawreh","doi":"10.3897/pharmacia.71.e114547","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e114547","url":null,"abstract":"Background: Chrysin is known for its pharmacological effects and structural resemblance to estrogen. The study explores the impact of chrysin on steroidogenesis focusing on adrenal and ovarian steroidogenic enzymes.\u0000 Materials and methods: Thirty female BALB/c mice were divided into three groups: a control group and two chrysin-treated groups (50 mg and 100 mg). Gene expression of key steroidogenic enzymes was assessed in adrenal glands and ovaries using RT-qPCR. Uterine expression of estrogen receptor alpha (ERα) was also examined. Histological analysis of adrenal glands and ovaries was performed.\u0000 Results: High-dose chrysin downregulated CYP17A1 expression in adrenal glands compared to control and low-dose groups. In contrast, 3β-HSD was significantly downregulated in the high-dose group. In ovaries, high dose chrysin reduced aromatase expression.\u0000 Conclusion: Our findings revealed that chrysin’s impact on steroidogenesis is dose-dependent. By downregulating CYP17A1 in adrenal glands, potentially affecting androgen and estrogen synthesis, and enhancing aromatase expression in ovaries at lower doses.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"1994 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140416977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of meloxicam, lornoxicam, ketoprofen, and dexketoprofen on human cervical, colorectal, and mammary carcinoma cell lines 美洛昔康、洛诺昔康、酮洛芬和右酮洛芬对人类宫颈癌、结直肠癌和乳腺癌细胞系的影响

Pharmacia Pub Date : 2024-02-29 DOI: 10.3897/pharmacia.71.e113677

Lyubomir Marinov, A. Georgieva, R. Toshkova, Ivanka Kostadinova, I. Mangarov, Tanya Toshkova-Yotova, Irina Nikolova

{"title":"The effects of meloxicam, lornoxicam, ketoprofen, and dexketoprofen on human cervical, colorectal, and mammary carcinoma cell lines","authors":"Lyubomir Marinov, A. Georgieva, R. Toshkova, Ivanka Kostadinova, I. Mangarov, Tanya Toshkova-Yotova, Irina Nikolova","doi":"10.3897/pharmacia.71.e113677","DOIUrl":"https://doi.org/10.3897/pharmacia.71.e113677","url":null,"abstract":"Targeting the inflammation-related molecules with nonsteroidal anti-inflammatory drugs (NSAIDs) represents a promising approach for cancer prevention/therapy. We evaluated the in vitro anticancer effects of meloxicam, lornoxicam, ketoprofen, and dexketoprofen on the proliferation, migration, and apoptosis of human cervical, colorectal, and mammary carcinoma cells. The antiproliferative activity and cytotoxicity of tested NSAIDs on HeLa, HT-29, and MCF-7 cell lines were assessed by the MTT test. The apoptosis-inducing potential was analyzed by fluorescent staining with acridine orange/ethidium bromide and DAPI. Migration activity was assessed by a wound-healing scratch assay. The tested NSAIDs reduced the viability of the used tumor cell lines. The cytomorphological analysis revealed reduced cell density and mitotic activity and the presence of cells with morphological features of early and late apoptosis. Significant inhibition of the migration capacity was established as well. In conclusion, NSAIDs could be candidates for the development of new pharmacological strategies for the treatment and prevention of cancer.","PeriodicalId":508564,"journal":{"name":"Pharmacia","volume":"657 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140417108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0