10%蜂胶牙膏在体外抑制生物膜形成细菌生长的潜力

Suryono Suryono, P. Setiawan, Nur Rahman Ahmad Seno Aji, Christia Aye Waindy Vega, Budi Rodestawati, Endang Lukitaningsih, Friska Ani Rahman, Sherlina Devina, Sholiha Sarah Tsabita, Selcaria Istna Datau, A. Alhasyimi
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引用次数: 0

摘要

研究目的研究10%蜂胶牙膏在体外抑制生物膜形成细菌生长的潜力。材料和方法评估感官特性,包括颜色、气味和味道。抗菌测试采用针对变异链球菌、金黄色葡萄球菌和牙龈卟啉单胞菌的盘扩散法,而细胞毒性则采用针对成纤维细胞的 3-(4,5-二甲基噻唑-2-基)-5-(3-羧基甲氧基苯基)-2-(4-磺酸苯基)-2H-四唑(MTS)检测法进行评估。统计分析包括平均值 ± 标准偏差。然后使用单因素方差分析和 Kruskal-Wallis 对数据进行检验,再进行事后检验(P < 0.05)。结果10%蜂胶牙膏的感官评估显示,其外观清晰,具有一致的橙味,香气可持续30天。根据抗菌结果,10%蜂胶牙膏样品可抑制变异链球菌、金黄色葡萄球菌和牙龈卟啉单胞菌的生长。事后检验表明,与阴性对照组相比,牙膏对变异链球菌和金黄色葡萄球菌有明显的抑制作用(p < 0.05)。与阴性对照相比,牙膏对牙龈脓杆菌的抑制面积更大,但没有观察到明显差异(p > 0.05)。对成纤维细胞进行的细胞毒性评估显示,存活细胞的比例很高(85.31%)。这些研究结果凸显了蜂胶牙膏作为口腔护理产品的10%的潜力和无毒性。结论10%蜂胶牙膏可抑制变形杆菌、金黄色葡萄球菌和牙龈脓胞的生长,对成纤维细胞无毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Potential of 10% propolis-based toothpaste on the inhibition of biofilm-forming bacteria growth in vitro
Aim of the study: To investigate the potential of 10% propolis-based toothpaste on inhibiting biofilm-forming bacteria growth in vitro. Material and method: Organoleptic properties are evaluated, considering color, odor, and taste. Antibacterial tests use a disc diffusion method against Streptococcus mutans, Staphylococcus aureus and Porphyromonas gingivalis bacteria, while cytotoxicity is assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay on fibroblast cells. Statistical analysis involves mean ± standard deviation. The data were then tested using a one-way analysis of variance and Kruskal-Wallis, followed by post-hoc test (p < 0.05). Results: The organoleptic evaluation of 10% propolis toothpaste reveals a visually clear appearance, consistent orange flavor, and aroma lasting 30 days. Based on the antibacterial results, a 10% level of propolis toothpaste sample inhibited the growth of Streptococcus mutans, Staphylococcus aureus and Porphyromonas gingivalis bacteria. The post-hoc test showed that toothpaste demonstrated significant inhibition on S. mutans and S. aureus compared to the negative control (p < 0.05). The toothpaste showed a larger inhibitory zone towards P. gingivalis compared to the adverse control; however, no significant differences were observed (p > 0.05). Cytotoxicity assessment on fibroblast cells shows a high percentage (85.31%) of viable cells. The findings highlight the 10% of propolis toothpaste’s potential and non-toxic as oral care product. Conclusions: 10% propolis toothpaste inhibits S. mutans, S. aureus, P. gingivalis growth, and not toxic on fibroblast.
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