Annual Review of Biophysics最新文献_第3页

Mitochondrial Outer Membrane Voltage-Dependent Anion Channels: Unique Structures, Distinct Functions, and Novel Therapeutic Targets. 线粒体外膜电压依赖性阴离子通道：独特的结构、独特的功能和新的治疗靶点。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2026-02-05 DOI: 10.1146/annurev-biophys-061124-102155

Shashank Ranjan Srivastava, Aadish Rawat, Radhakrishnan Mahalakshmi

{"title":"Mitochondrial Outer Membrane Voltage-Dependent Anion Channels: Unique Structures, Distinct Functions, and Novel Therapeutic Targets.","authors":"Shashank Ranjan Srivastava, Aadish Rawat, Radhakrishnan Mahalakshmi","doi":"10.1146/annurev-biophys-061124-102155","DOIUrl":"10.1146/annurev-biophys-061124-102155","url":null,"abstract":"Voltage-dependent anion channels (VDACs) of the outer mitochondrial membrane carry out bidirectional flux of metabolites and ions and serve as the first line of communication between the cytosol and mitochondria. They are now recognized as indispensable for mitochondrial function and cellular homeostasis, mitochondria-endoplasmic reticulum communication, lipid and cholesterol biogenesis, Ca2+ homeostasis, and mitochondria-mediated apoptosis. The unique structural features of VDACs are also important in redox regulation. VDAC dysregulation by interaction with amyloid-β, α-synuclein, Tau, or tubulin can lead to neurodegeneration. Here, we provide insights into the structures, isoform-specific molecular functions, cellular interactome, variations, and unique regulatory elements of VDACs and their direct implications for widespread burdens like cancer and neurodegeneration in humans. We discuss how deducing isoform-specific structure-function studies of VDACs has the potential for successful development of next-generation diagnostics-guided therapeutics.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":" ","pages":"391-414"},"PeriodicalIF":12.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146127379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pattern Formation in Cell Cultures. 细胞培养中的模式形成。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2026-01-20 DOI: 10.1146/annurev-biophys-030822-031838

Kaushik Roy, Aryeh Warmflash

{"title":"Pattern Formation in Cell Cultures.","authors":"Kaushik Roy, Aryeh Warmflash","doi":"10.1146/annurev-biophys-030822-031838","DOIUrl":"10.1146/annurev-biophys-030822-031838","url":null,"abstract":"Pattern formation, or the emergence of spatial order and fate specification from initially homogeneous cell populations, is a fundamental problem in developmental biology and biophysics. In vitro cell culture platforms now provide powerful tools to investigate the mechanisms of pattern formation under controlled conditions. In this review, we present an integrated perspective on recent advances in pattern formation studies across a diverse array of in vitro systems, organized thematically by the experimental platforms they employ and the underlying principles they reveal, ranging from biochemical gradients to dynamic signaling and mechanochemical feedback. We discuss how these systems recapitulate principles such as morphogen gradients and reaction-diffusion dynamics while enabling mechanistic dissection of self-organization. Particular emphasis is placed on stem cell-based models of early human development that provide unique access to early developmental patterning. We further explore how dynamic signaling, collective behavior, and multisignal integration define emergent patterning phenomena. Finally, we outline future challenges and opportunities in combining theoretical and experimental approaches to model and engineer spatial organization in multicellular systems.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":" ","pages":"199-224"},"PeriodicalIF":12.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146012511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the Single Molecule: Multiplexed Methods in Force Spectroscopy. 超越单分子：力谱中的多路方法。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2026-01-02 DOI: 10.1146/annurev-biophys-021424-124520

Ken Halvorsen, Andrew Ward, Wesley P Wong

{"title":"Beyond the Single Molecule: Multiplexed Methods in Force Spectroscopy.","authors":"Ken Halvorsen, Andrew Ward, Wesley P Wong","doi":"10.1146/annurev-biophys-021424-124520","DOIUrl":"10.1146/annurev-biophys-021424-124520","url":null,"abstract":"Single-molecule techniques have transformed biological research by enabling direct observation and manipulation of individual molecules. These methods overcome ensemble averaging inherent in bulk measurements and facilitate studies under physiological stresses and out-of-equilibrium conditions. They have provided valuable insights into diverse biological processes, from stepping mechanisms of molecular motors to mechanical properties of biomolecules to the dynamic strength of intermolecular bonds. Advances in multiplexed and high-throughput single-molecule force spectroscopy methods are improving throughput, capabilities, and accessibility. In this review, we detail the evolution of multiplexed force spectroscopy technologies, highlighting key advances in instrumentation, molecular engineering, and analytical techniques. We discuss diverse applications spanning molecular biophysics, biomolecular sensing, proteomics, and cellular mechanobiology. Finally, we explore ongoing challenges and future opportunities and highlight how the impact of multiplexed single-molecule force spectroscopy can continue to grow through further developments in novel instrumentation, chemical tools, and innovative applications.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":" ","pages":"135-155"},"PeriodicalIF":12.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12997103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145892966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioenergetics and the Evolution of Cellular Traits. 生物能量学与细胞特性的进化。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2025-05-01 DOI: 10.1146/annurev-biophys-070524-090334

Paul E Schavemaker, Michael Lynch

引用次数: 0

Collapse and Protein Folding: Should We Be Surprised That Biothermodynamics Works So Well? 崩溃与蛋白质折叠：我们是否应该对生物热力学如此有效感到惊讶？

IF 13.7 1区生物学

Annual Review of Biophysics Pub Date : 2025-05-01 Epub Date: 2024-12-17 DOI: 10.1146/annurev-biophys-080124-123012

Tobin R Sosnick, Michael C Baxa

{"title":"Collapse and Protein Folding: Should We Be Surprised That Biothermodynamics Works So Well?","authors":"Tobin R Sosnick, Michael C Baxa","doi":"10.1146/annurev-biophys-080124-123012","DOIUrl":"10.1146/annurev-biophys-080124-123012","url":null,"abstract":"A complete understanding of protein function and dynamics requires the characterization of the multiple thermodynamic states, including the denatured state ensemble (DSE). Whereas residual structure in the DSE (as well as in partially folded states) is pertinent in many biological contexts, here we are interested in how such structure affects protein thermodynamics. We examine issues related to chain collapse in light of new developments, focusing on potential complications arising from differences in the DSE's properties under various conditions. Despite some variability in the degree of collapse and structure in the DSE, stability measurements are remarkably consistent between two standard methods, calorimetry and chemical denaturation, as well as with hydrogen-deuterium exchange. This robustness is due in part to the DSEs obtained with different perturbations being thermodynamically equivalent and hence able to serve as a common reference state. An examination of the properties of the DSE points to it as being a highly expanded ensemble with minimal amounts of stable hydrogen bonded structure. These two features are likely to be critical in the broad and successful application of thermodynamics to protein folding. Our review concludes with a discussion of the impact of these findings on folding mechanisms and pathways.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":" ","pages":"17-34"},"PeriodicalIF":13.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Physics of Sensing and Decision-Making by Animal Groups. 动物群体感知和决策的物理学。

IF 13.7 1区生物学

Annual Review of Biophysics Pub Date : 2025-05-01 DOI: 10.1146/annurev-biophys-061824-110733

Danielle L Chase, Orit Peleg

{"title":"The Physics of Sensing and Decision-Making by Animal Groups.","authors":"Danielle L Chase, Orit Peleg","doi":"10.1146/annurev-biophys-061824-110733","DOIUrl":"10.1146/annurev-biophys-061824-110733","url":null,"abstract":"To ensure survival and reproduction, individual animals navigating the world must regularly sense their surroundings and use this information for important decision-making. The same is true for animals living in groups, where the roles of sensing, information propagation, and decision-making are distributed on the basis of individual knowledge, spatial position within the group, and more. This review highlights key examples of temporal and spatiotemporal dynamics in animal group decision-making, emphasizing strong connections between mathematical models and experimental observations. We start with models of temporal dynamics, such as reaching consensus and the time dynamics of excitation-inhibition networks. For spatiotemporal dynamics in sparse groups, we explore the propagation of information and synchronization of movement in animal groups with models of self-propelled particles, where interactions are typically parameterized by length and timescales. In dense groups, we examine crowding effects using a soft condensed matter approach, where interactions are parameterized by physical potentials and forces. While focusing on invertebrates, we also demonstrate the applicability of these results to a wide range of organisms, aiming to provide an overview of group behavior dynamics and identify new areas for exploration.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":"54 1","pages":"329-351"},"PeriodicalIF":13.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward Principles of Brain Network Organization and Function. 脑网络组织与功能原理探讨。

IF 13.7 1区生物学

Annual Review of Biophysics Pub Date : 2025-05-01 Epub Date: 2025-02-14 DOI: 10.1146/annurev-biophys-030722-110624

Suman Kulkarni, Dani S Bassett

{"title":"Toward Principles of Brain Network Organization and Function.","authors":"Suman Kulkarni, Dani S Bassett","doi":"10.1146/annurev-biophys-030722-110624","DOIUrl":"10.1146/annurev-biophys-030722-110624","url":null,"abstract":"The brain is immensely complex, with diverse components and dynamic interactions building upon one another to orchestrate a wide range of behaviors. Understanding patterns of these complex interactions and how they are coordinated to support collective neural function is critical for parsing human and animal behavior, treating mental illness, and developing artificial intelligence. Rapid experimental advances in imaging, recording, and perturbing neural systems across various species now provide opportunities to distill underlying principles of brain organization and function. Here, we take stock of recent progress and review methods used in the statistical analysis of brain networks, drawing from fields of statistical physics, network theory, and information theory. Our discussion is organized by scale, starting with models of individual neurons and extending to large-scale networks mapped across brain regions. We then examine organizing principles and constraints that shape the biological structure and function of neural circuits. We conclude with an overview of several critical frontiers, including expanding current models, fostering tighter feedback between theory and experiment, and leveraging perturbative approaches to understand neural systems. Alongside these efforts, we highlight the importance of contextualizing their contributions by linking them to formal accounts of explanation and causation.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":" ","pages":"353-378"},"PeriodicalIF":13.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryo-EM of Mitochondrial Complex I and ATP Synthase. 线粒体复合体I和ATP合酶的低温电镜分析。

IF 13.7 1区生物学

Annual Review of Biophysics Pub Date : 2025-05-01 DOI: 10.1146/annurev-biophys-060724-110838

Werner Kühlbrandt, Luis A M Carreira, Özkan Yildiz

引用次数: 0

Metabolic Engineering of Yeast. 酵母代谢工程。

IF 13.7 1区生物学

Annual Review of Biophysics Pub Date : 2025-05-01 Epub Date: 2025-01-21 DOI: 10.1146/annurev-biophys-070924-103134

Shuobo Shi, Yu Chen, Jens Nielsen

引用次数: 0

Membrane Association of Intrinsically Disordered Proteins. 内在无序蛋白的膜结合。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2025-05-01 Epub Date: 2025-02-14 DOI: 10.1146/annurev-biophys-070124-092816

Matthew MacAinsh, Fidha Nazreen Kunnath Muhammedkutty, Ramesh Prasad, Huan-Xiang Zhou

引用次数: 0