Burns最新文献

{"title":"Prognostic value of red blood cell distribution width/protein and red blood cell distribution width/platelet count ratios in intensive care burn patients","authors":"Doha Obed ,&nbsp;Huu H. Nguyen ,&nbsp;Mustafa Salim ,&nbsp;Nadjib Dastagir ,&nbsp;Martynas Tamulevicius ,&nbsp;Florian Bucher ,&nbsp;Anieto O.M. Enechukwu ,&nbsp;Peter M. Vogt","doi":"10.1016/j.burns.2026.107851","DOIUrl":"10.1016/j.burns.2026.107851","url":null,"abstract":"<div><h3>Background</h3><div>Red cell distribution width (RDW), serum protein levels, platelet count (PLT), and RDW/protein and RDW/PLT ratios have been associated with adverse outcomes in various medical conditions such as sepsis and critical illness. This study aimed to investigate the association between these parameters and in-hospital mortality following severe burn injury.</div></div><div><h3>Methods</h3><div>Intensive care burn patients admitted between March 2007 and December 2020 with a total body surface area (TBSA) burned ≥ 20 % were analyzed. Multivariate analysis was conducted to examine variables associated with mortality. RDW/protein and RDW/PLT ratios on post-burn days 1, 3 and 7 were analyzed by receiver operating characteristic curves.</div></div><div><h3>Results</h3><div>A total of 288 patients were included. Mortality was 26 %, with non-survivors being significantly older, more frequently male, and presenting with an average of 49 % of TBSA burned. RDW, serum protein levels and PLT were not independent predictive values. The RDW/protein ratios on post-burn days 3 and 7 were significantly associated with in-hospital mortality. The respective AUC value exceeded 0.8 on post-burn day 7, indicating good predictive ability.</div></div><div><h3>Conclusion</h3><div>Post-burn evaluation of the RDW/protein ratio may provide a simple, cost-effective, and valuable predictor for mortality risk assessment following burn injury.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"52 2","pages":"Article 107851"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of COVID-19 on patterns of burns evaluated in pediatric emergency departments","authors":"Stephanie Ruest ,&nbsp;Laurie H. Johnson ,&nbsp;Katherine T. Flynn-O’Brien ,&nbsp;Christina Georgeades ,&nbsp;Holly R. Hanson ,&nbsp;Andrew W. Kiragu ,&nbsp;Michelle T. Ruda ,&nbsp;Mark R. Zonfrillo ,&nbsp;Wendy J. Pomerantz","doi":"10.1016/j.burns.2025.107823","DOIUrl":"10.1016/j.burns.2025.107823","url":null,"abstract":"<div><h3>Background</h3><div>Fire- and burn-related injuries are among the top 10 causes of death for children &lt; 18 years in the United States and are associated with significant morbidity. Data regarding the impact of the COVID-19 pandemic on pediatric burns in emergency department (ED) settings are limited.</div></div><div><h3>Objective</h3><div>To examine differences in pediatric burn-related injury patterns before and during the COVID-19 pandemic.</div></div><div><h3>Methods</h3><div>This is a subanalysis of a multi-center cross-sectional study of 741,418 injury-related visits to 40 pediatric EDs for children &lt; 18 years. Burn visits were identified by International Classification of Diseases, 10th Revision (ICD-10) codes. Patient demographics and burn-related injury patterns for 3/17/2019–12/31/2019 (control period) vs. 3/15/2020–12/31/2020 (pandemic study period) were compared.</div></div><div><h3>Results</h3><div>Burn-related visits comprised 2.2 % (N = 12,908) of all injury visits. In 2020 there were 3.7 % fewer burn visits, but the proportion of burn visits relative to other injuries was higher (2.5 % vs. 2.0 %, p &lt; 0.001). There were significant differences in burn visits in 2020 by race (p &lt; 0.05) and ethnicity (p = 0.01), with higher proportions of White and non-Hispanic patients. There were significant differences in the geographic distribution of burn visits in 2020 (p &lt; 0.001) and significantly higher proportions of more urgent triage levels (p &lt;0.05) and trauma activations (p &lt; 0.05). Body region affected (p &lt; 0.01), intent (p &lt; 0.001) and mechanism (p &lt; 0.001) also differed significantly. A higher proportion were due to flames/fires and fewer due to hot objects.</div></div><div><h3>Conclusions</h3><div>Burns comprised a higher proportion of injury-related ED visits during the pandemic, with significant differences in patient demographics and injury patterns. These results can inform burn prevention education.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"52 2","pages":"Article 107823"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145835335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comments on: “Evaluation of the effect of post-burn pruritus on patients’ quality of life and experiences”","authors":"Runze Zhang ,&nbsp;Chenlu Zhao ,&nbsp;Maocan Tao","doi":"10.1016/j.burns.2025.107832","DOIUrl":"10.1016/j.burns.2025.107832","url":null,"abstract":"","PeriodicalId":50717,"journal":{"name":"Burns","volume":"52 2","pages":"Article 107832"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and analysis of the skin mycobiome in keloid: A case-control study","authors":"Yuyan Yang ,&nbsp;Mengyuan Zhang ,&nbsp;Yixin Sun ,&nbsp;Tianhao Li ,&nbsp;Junxian Wen ,&nbsp;Zhijin Li ,&nbsp;Lin Jin ,&nbsp;Yakun Bi ,&nbsp;Wei Wei ,&nbsp;Ang Zeng ,&nbsp;Xiaojun Wang ,&nbsp;Nanze Yu ,&nbsp;Xiao Long","doi":"10.1016/j.burns.2025.107839","DOIUrl":"10.1016/j.burns.2025.107839","url":null,"abstract":"<div><div>Altered skin bacteriome has been associated with keloid, yet the relation of fungal community to this disease remains unclear. This study aimed to characterize the skin mycobiome in patients with keloid and its correlation to disease severity. A case-control study was designed to investigate the fungal landscape in participants with keloid and healthy controls. Disease severity was assessed using Vancouver Scar Scale. Swabs of the keloid lesion (KL) and non-lesional (KNL) skin of keloid patients and corresponding skin sites of healthy controls (C) were obtained for ITS rRNA sequencing. Our findings revealed decreased richness and evenness in the fungal community within the KL as well as KNL groups compared to C (P &lt; 0.01). Notably, fungal composition was more similar between KNL and C compared to KL (KL-C, ANOSIM: R=0.12, P = 0.004; KL-KNL, ANOSIM: R=0.10, P = 0.021; KNL-C, ANOSIM: R=0.03, P = 0.215). Skin mycobiome in KL was more enriched with <em>Malassezia</em> (P &lt; 0.05), and depleted in <em>Cladosporium</em>, <em>Alternaria</em>, <em>Aspergillus</em> and <em>Debaryomyces</em> (P &lt; 0.05). Correlation analysis identified a positive association between the relative abundance of <em>Malassezia</em> and disease severity (r_s=0.56, P = 0.007). The present study shows a decreased fungal diversity and altered fungal composition structure in keloid lesions. The overabundance of <em>Malassezia</em> and its correlation to disease severity highlight a potential role of these fungi in the pathogenesis of keloid and offer insights into novel disease-management approaches.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"52 2","pages":"Article 107839"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased systemic heat-shock protein 27 and 70 following severe burn injury","authors":"Bastian Garmroudi ,&nbsp;Thomas Haider ,&nbsp;Carmen Haider ,&nbsp;Philipp Hacker ,&nbsp;Gregor Werba ,&nbsp;Hendrik Jan Ankersmit ,&nbsp;Stefan Hacker","doi":"10.1016/j.burns.2025.107846","DOIUrl":"10.1016/j.burns.2025.107846","url":null,"abstract":"<div><h3>Background</h3><div>Severe burn injuries trigger a complex systemic response, including dysregulated inflammation and immunosuppression. Heat shock proteins (HSPs) regulate cellular stress responses and have immunomodulatory functions when released into the extracellular compartment. Our aim was to investigate the release of systemic HSP27 and 70 in patients suffering from severe burn injury.</div></div><div><h3>Methods</h3><div>We analyzed serum HSP27 and HSP70 concentrations in 32 burn patients admitted to our burn intensive care unit with a total body surface area (TBSA) affected of &gt; 10 % in comparison to eight healthy control probands. HSP27 and 70 concentrations were determined serially for four weeks using enzyme-linked immunosorbent assays (ELISA).</div></div><div><h3>Results</h3><div>The mean TBSA was 32.5 % ± 19.6 %, and in-hospital mortality occurred in six patients (18.8 %). HSP27 and HSP70 concentrations were significantly elevated in burn patients compared to controls, with peak concentrations on the day of admission (HSP27: 590 ± 335 vs. 83 ± 42 pg/mL, p &lt; 0.001; HSP70: 1961 ± 2214 vs. 189 ± 130 pg/mL, p &lt; 0.001). HSP70 concentrations were significantly higher in non-survivors (1143 vs. 946 pg/mL, p &lt; 0.05) and were significantly higher in patients with more than 29 % TBSA compared to patients with lower TBSA (p &lt; 0.01). Receiver operating characteristic curve analysis identified HSP70 measured on the day of admission as a significant predictor of patient mortality.</div></div><div><h3>Conclusion</h3><div>Severe thermal trauma results in elevated HSP27 and HSP70 concentrations. Increased HSP70 levels are associated with higher in-hospital mortality in burn patients, suggesting potential use as a prognostic biomarker.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"52 2","pages":"Article 107846"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Educational impact of a cost-efficient burns excision and skin grafting porcine simulation model","authors":"Ameen Mahmood ,&nbsp;Ayushi Gianchandani ,&nbsp;Cheuk Ying Kyleen Kiew ,&nbsp;Anuska Shah ,&nbsp;Hasan Zaidi ,&nbsp;Abith Kamath ,&nbsp;Rananjay Singh ,&nbsp;Nikita Kartikapallil ,&nbsp;Marya Abuarqoub ,&nbsp;Daniel Markeson","doi":"10.1016/j.burns.2025.107836","DOIUrl":"10.1016/j.burns.2025.107836","url":null,"abstract":"<div><h3>Introduction</h3><div>Burns education is underrepresented in UK undergraduate and early postgraduate medical training, despite being a core emergency presentation. This limits exposure to essential principles and skills. Porcine skin is anatomically similar to human tissue and cost-efficient, offering a valuable tool. This study evaluates the educational impact of a low-cost burns excision and skin-grafting porcine-based simulation model.</div></div><div><h3>Methods</h3><div>A prospective cohort study was conducted through a workshop comprising a lecture on burns followed by hands-on practice using porcine models. Participants completed pre- and post-workshop surveys, including multiple choice questions and evaluated self-rated confidence and perceptions using five-point Likert scales. Technical skills were assessed using supervisor-rated Objective Structured Assessment of Technical Skills (OSATS). Statistical analysis utilised Wilcoxon signed-rank and McNemar’s Tests.</div></div><div><h3>Results</h3><div>Thirty participants (28 medical students and two resident doctors) with no prior experience in burns management or grafting partook in the workshop. Theoretical knowledge significantly improved from 1.8 ± 0.7 to 3.9 ± 0.9 (p &lt; 0.01), and surgical skills increased from 1.6 ± 0.7 to 3.6 ± 1.0 (p &lt; 0.01). OSATS scoring showed significant improvements across all domains (p &lt; 0.01). Knowledge-based assessments improved significantly in graft layers, total body surface area (TBSA), and viability assessment (p &lt; 0.01). Participants rated the workshop's effectiveness score at 4.6 ± 0.7 and recommendation likelihood at 4.3 ± 0.5.</div></div><div><h3>Conclusions</h3><div>The model significantly improves knowledge, confidence, and technical skills in burns management and grafting.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"52 2","pages":"Article 107836"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145835332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of hemoglobin levels and mortality in burn patients","authors":"Qinghua Wu ,&nbsp;Jingwei Zhang ,&nbsp;Caoyi Liu ,&nbsp;Zhiyong Huang","doi":"10.1016/j.burns.2025.107840","DOIUrl":"10.1016/j.burns.2025.107840","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the correlation between hemoglobin (Hb) levels and 1-year mortality in burn patients in intensive care units.</div></div><div><h3>Methods</h3><div>We conducted a retrospective cohort study, extracting relevant data from the American Intensive Care Unit (ICU) Medical Information Database IV (MIMIC-IV) for burn patients. This data included complete blood counts, biochemistry and coagulation parameters (both maximum and minimum values during ICU hospitalization), Simplified Acute Physiology Score II (SAPSII), Acute Physiology ScoreIII (APSIII), Oxford Acute　Severity of Illness Score (Oasis), Sequential Organ Failure Assessment (SOFA), comorbidities, and outcome. A restricted cubic spline model was utilized to illustrate the relationship between Hb levels and 1-year mortality risk. Patients were stratified into three groups based on gender and the relationship curve (Female: Hb&lt;10.0 g/dL, 10.0 g/dL≤Hb&lt;11.5 g/dL, Hb≥11.5 g/dL; Male: Hb&lt;11.0 g/dL, 11.0 g/dL≤Hb&lt;12.5 g/dL, Hb≥12.5 g/dL). Logistic regression models were employed to assess one-year mortality risk in each group, while sensitivity analysis was conducted via subgroup analysis and propensity score matching (PSM) analysis.</div></div><div><h3>Results</h3><div>A total of 278 burn patients were included in the study, revealing a one-year mortality rate of 12.2 %. Classifying the patients according to their Hb levels in 3 groups, the numerical mortality rates was different (Q1:22.1 %, Q2:8.9 %, Q3:7.0 %). Whether treating Hb as a continuous or categorical variable, both univariate and multivariate logistic regression analyses indicated a gradual decrease in mortality as Hb levels increased. In models adjusted for all confounding factors, the mortality risk for the second and third Hb groups was significantly lower compared to the first group (OR=0.29, 95 % CI=0.10–0.79, p = 0.016; vs. OR=0.36, 95 % CI=0.14–0.93, p = 0.035). Subgroup analysis displayed consistent correlations between reduced Hb levels and increased mortality across various ages, genders, and comorbidities, including chronic pulmonary disease, cerebrovascular disease, liver disease, kidney diseases, and diabetes. After stratifying patients into two groups (Female: Hb&lt;11.0 g/dL and Hb≥11.0 g/dL; Male: Hb&lt;12.0 g/dL and Hb≥12.0 g/dL) and adjusting for potential confounders using Propensity Score Matching (PSM) analysis, as well as applying propensity score adjustment and weighted pairwise analysis (PA), the results remained stable (OR=0.34, CI=0.14–0.83, p = 0.017).</div></div><div><h3>Conclusion</h3><div>Lower Hb levels in burn patients during ICU hospitalization are associated with higher one-year mortality.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"52 2","pages":"Article 107840"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"optiTHERMM: A trainee-led international collaborative survey on perioperative patient body temperature monitoring and management for major burn injuries","authors":"Guy H.M. Stanley ,&nbsp;Majid Al-Khalil ,&nbsp;Cody C. Frear ,&nbsp;Glenn Boardman ,&nbsp;Lorna A. Burrows ,&nbsp;Skylar Paulich ,&nbsp;Jennifer C. Field ,&nbsp;Jonathon M. Pleat ,&nbsp;The optiTHERMM collaborators","doi":"10.1016/j.burns.2025.107817","DOIUrl":"10.1016/j.burns.2025.107817","url":null,"abstract":"<div><h3>Introduction</h3><div>Major burn injuries are often accompanied by disturbances in thermoregulation, with perioperative hypo- and hyperthermia presenting significant challenges for clinicians. Despite its implications for patient outcomes, research into perioperative thermoregulation and its influence on surgical decision-making remains limited.</div></div><div><h3>Methods</h3><div>Between February 2022 and June 2023, a 26-question survey was distributed to clinicians in the UK, Australia, and New Zealand to assess body temperature management practices for major burn patients. Respondents included burn surgeons, anaesthetists, and intensive care specialists, categorised by burn service complexity and patient demographics.</div></div><div><h3>Results</h3><div>A total of 148 responses were collected, with 96 from the UK (22 sites) and 52 from Australia and New Zealand (22 sites), achieving a response rate of 70 % among designated burn services (39/56). Most clinicians reported an absence (38 %) or lack of awareness (22 %) regarding local thermoregulation protocols. Hypothermia was more likely than hyperthermia to cause surgery delays, with 35° C cited as the most common threshold. No significant differences were found in temperature thresholds based on service complexity (p = 0.324), patient demographics (p = 0.67), or clinician roles (p = 0.238). Respondents from high-complexity hospitals reported significantly lower minimum and higher maximum preferred body temperature thresholds <em>during</em> surgery (p = 0.008 and p = 0.02, respectively). However, these thresholds did not differ significantly based on patient population (p = 0.259; p = 0.897) or clinician role (p = 0.409; p = 0.283). Methods used to monitor and manage body temperature were broadly consistent with those cited in other studies.</div></div><div><h3>Conclusions</h3><div>This international study represents the largest investigation of thermoregulation practices in major burns to date. While a consensus on optimal monitoring and modulation methods exists, there is considerable variability in practices and temperature preferences, highlighting the need for evidence-based guidelines to ensure consistent and optimal care.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"52 2","pages":"Article 107817"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of socioeconomic deprivation on the adequacy of burn first aid: a retrospective cohort study","authors":"Man Hin Kong ,&nbsp;Alexander J. Baldwin ,&nbsp;Ryan Kerstein","doi":"10.1016/j.burns.2026.107864","DOIUrl":"10.1016/j.burns.2026.107864","url":null,"abstract":"<div><h3>Aim</h3><div>This study aimed to examine the relationship between socioeconomic status, measured by the Index of Multiple Deprivation (IMD), and the adequacy of first aid provided for burn injuries.</div></div><div><h3>Methods</h3><div>A retrospective cohort study was conducted including all patients (paediatric and adult) treated for thermal burns at a regional burn unit between 1 May and 31 July 2024. Socioeconomic status was assessed using IMD deciles. First aid was categorised as “adequate” (cooling with water for ≥20 min) or “inadequate,” with inadequate first aid further classified as “damaging” (e.g., ice, toothpaste, raw egg) or “not damaging.” Logistic regression analyses adjusted for age, sex, burn aetiology and size of burn.</div></div><div><h3>Results</h3><div>269 patients were included (median age 28.3 years; 66.9 % adults; 50.9 % male). Scalds were the most common aetiology (59.5 %), and most burns were superficial partial thickness (82.5 %). The median IMD decile was 7 (IQR 5–9). Increasing socioeconomic deprivation (lower IMD decile) was significantly associated with inadequate first aid (OR 0.898 [per unit decrease in IMD decile] 95 % CI 0.807–0.998, p = 0.046). Greater deprivation was also associated with increased odds of harmful first aid practices, compared with adequate first aid (RRR 0.836, 95 % CI 0.737–0.948, p = 0.005).</div></div><div><h3>Conclusion</h3><div>Socioeconomic deprivation is inversely associated with adequate burn first aid and positively associated with harmful practices. Targeted public health interventions are needed to address this disparity.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"52 2","pages":"Article 107864"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors affecting skin keratinocyte and fibroblast extraction yields for the production of living skin substitutes to treat severely burned patients","authors":"Ludivine Dubourget ,&nbsp;Danielle Larouche ,&nbsp;Sergio Cortez Ghio ,&nbsp;Véronique J. Moulin ,&nbsp;Chanel Beaudoin-Cloutier ,&nbsp;Lucie Germain","doi":"10.1016/j.burns.2025.107805","DOIUrl":"10.1016/j.burns.2025.107805","url":null,"abstract":"<div><h3>Backgrounds</h3><div>As autografting is limited for severely burned patients due to a lack of healthy donor sites, tissue-engineered autologous skin substitutes have emerged as a promising alternative. Yet, challenges persist, particularly regarding production time. Since cell culture is influenced by multiple factors, identifying these factors is crucial for improving culture yields. This retrospective study aimed to identify factors affecting skin cell extraction yields.</div></div><div><h3>Methods</h3><div>Culture data (method used, etc.) and clinical data (medical history, etc.) from all available patient records over a 35-year-period were collected. 18 variables were assessed using XGBoost as a variable selection tool, before fitting mixed-effects multivariate linear modeling.</div></div><div><h3>Results</h3><div>As expected, age inversely correlated with keratinocyte and fibroblast extraction yields, decreasing by 0.048 × 10⁶ cells/cm² per year (CI_95 % = [-0.065;-0.031]) and 0.035 × 10⁶ cells/cm² per year (CI_95 % = [-0.050;-0.019]), respectively. Keratinocyte yield also rose by 0.936 × 10⁶ cells/cm² (CI_95 % = [0.175;1.697]) when hairs could be grasped during the epidermis-dermis separation. Conversely, fibroblast yield increased by 0.042 × 10⁶ cells/cm² per day post-burn (CI_95 % = [0.007;0.076]) and by 0.019 × 10⁶ cells/cm² per percentage of TBSA burned (CI_95 % = [0.002;0.036]).</div></div><div><h3>Conclusions</h3><div>This findings provide valuable insights into factors influencing skin cell extraction yields, which may help optimize skin biopsy parameters, ultimately improving production efficiency.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"52 2","pages":"Article 107805"},"PeriodicalIF":2.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}