Burns最新文献

{"title":"Post-transcriptional regulation of diabetic wound healing by junctional adhesion molecule A/miR-106b axis","authors":"Teng Gong ,&nbsp;Xiaoming Fan ,&nbsp;Minjuan Wu ,&nbsp;Zhaohong Chen ,&nbsp;Zhaofan Xia","doi":"10.1016/j.burns.2025.107527","DOIUrl":"10.1016/j.burns.2025.107527","url":null,"abstract":"<div><h3>Background</h3><div>Despite advancements in molecular science and biomaterial technology, the mechanisms underlying the impaired healing of diabetic wounds remain unclear. In this study, we investigated the post-transcriptional regulation of diabetic wound healing using JAM-A.</div></div><div><h3>Methods</h3><div>Mouse wound models, hematoxylin and eosin staining analysis, and scratch wound assays were used to investigate the effects of JAM-A 3′-UTR on the re-epithelialization of diabetic wounds, whereas RNA pulldown, microRNA-seq, and bioinformatics analyses were performed to identify key miRNA players and predict their target genes. <em>In situ</em> hybridization, immunohistochemistry, western blotting, and polymerase chain reaction (PCR) were used to confirm the alternative splicing of JAM-A 3′-UTR in diabetic conditions. CCK-8 proliferation assays, scratch wound assays, PCR, western blotting, and dual-luciferase assays were performed to study the changes in cell proliferation and migration induced by miR-106b-5p modification and confirm the target gene.</div></div><div><h3>Results</h3><div>JAM-A 3′-UTR accelerated re-epithelialization in diabetic mouse wounds. Shortened splicing was found in the 3′-UTR of JAM-A under diabetic conditions, leading to the excessive release of miR-106b-5p while the promoter of miR-106b was activated. Furthermore, upregulated miR-106b-5p over-activated cell proliferation and inhibited cell migration in diabetic wound keratinocytes by suppressing the target gene <em>PTEN/TIAM1</em> and regulating the AKT and RAC1 pathways, thereby impairing wound re-epithelialization.</div></div><div><h3>Conclusions</h3><div>We identified alternative splicing of JAM-A 3′-UTR in diabetic conditions, which caused the excessive release of miR-106b. Upregulation of miR-106b reduced the expression of its target genes, <em>PTEN</em> and <em>TIAM1</em>, which led to hyperactive proliferation and impaired migration of keratinocytes, thereby dysregulating wound re-epithelialization.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 6","pages":"Article 107527"},"PeriodicalIF":3.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psychological assessment of adults with burn injuries in Lebanon","authors":"Marcella Younes ,&nbsp;Joelle Saba ,&nbsp;Maria Nasr ,&nbsp;Pascale Salameh ,&nbsp;Wadih Naja","doi":"10.1016/j.burns.2025.107531","DOIUrl":"10.1016/j.burns.2025.107531","url":null,"abstract":"<div><h3>Background and objectives</h3><div>Burned patients are frequently diagnosed with psychiatric disorders. This study aimed to determine the prevalence of probable post traumatic stress disorder (PTSD), and to assess growth and self-esteem among burned patients.</div></div><div><h3>Subjects and methods</h3><div>A cross-sectional quantitative study was performed, investigating the prevalence of probable PTSD among burned inpatients or outpatients over six years compared to a control group. 230 adult participants of whom 133 were burned patients and 97 non-burned subjects participated in the study.</div></div><div><h3>Results</h3><div>Inpatients exhibited higher PTSD Checklist for DSM-5 (PCL-5) scores (49.80 ± 23.44) when compared to outpatients (34.64 ± 31.15) and to the control group (42.73 ± 25.66) but regression analysis failed to show a statistical significance among the different groups. 18 % of burned patients met probable PTSD status based on the PCL-5. 10 % (10/102) of burned inpatients, 7 % (2/31) of burned outpatients and 9 % (9/97) of control group reported low self-esteem. Outpatients had significantly higher Rosenberg self-esteem scores (22.09 ± 4.53) compared to inpatients (18.38 ± 3.90) and the control group (p-value = 0.000). A lower educational level was associated with higher probable PTSD and a younger age was associated with higher post-traumatic growth.</div></div><div><h3>Conclusion</h3><div>Burned patients may develop low self-esteem, but they have been found to have high resilience and well-established personal growth.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 6","pages":"Article 107531"},"PeriodicalIF":3.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of allogeneic blood transfusion on burn mortality in a resource-limited setting","authors":"Melissa Issa-Boube ,&nbsp;Jotham Gondwe ,&nbsp;Jared Gallaher ,&nbsp;Anthony Charles","doi":"10.1016/j.burns.2025.107526","DOIUrl":"10.1016/j.burns.2025.107526","url":null,"abstract":"<div><h3>Introduction</h3><div>There are nearly 9 million new burn cases worldwide, with a disproportional burn burden in low- and middle-income countries. Patients with significant burn injury frequently require multiple blood transfusions; however, there is a paucity of data regarding the effect of allogeneic blood transfusion following burn injury in a resource-limited setting with a high anemia prevalence at baseline. This study aimed to determine the effect of blood transfusion on burn mortality.</div></div><div><h3>Methods</h3><div>We performed a retrospective review of patients presenting with burns between 2011 and 2019, using prospectively collected burn registry data from Kamuzu Central Hospital (KCH). We performed multivariate logistic regression modeling to identify predictors of mortality, and we considered potential confounders.</div></div><div><h3>Results</h3><div>A total of 2359 patients were included. Mean age was 10 ± 14 with a male preponderance (58 %). The mean percent total body surface area burned (%TBSA) was 17.52 ± 14.46. 60 % of burns were caused by scald injuries and 37 % by flame. Below 40 % TBSA, our model predicted a higher probability of mortality for those transfused. However, above 40 % TBSA, the predicted probability of mortality is decreased for those transfused.</div></div><div><h3>Conclusions</h3><div>Allogeneic blood transfusion confers 1.23 (p &lt; 0.03) times higher odds of mortality in burn patients. Mortality risk increased with age and units transfused. This study highlights the need for proper guidelines and protocols for allogeneic blood transfusion in burn patients. A more restrictive blood transfusion strategy may be more appropriate in a resource-limited setting.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 6","pages":"Article 107526"},"PeriodicalIF":3.2,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of biomaterials in burn treatment: Comparative study of collagen-derived membranes, hydrogel, and sponge in an animal model","authors":"Leticia Ramos Dantas ,&nbsp;Eduardo Buscarons de Andrade ,&nbsp;Giovana Neves Tagliari ,&nbsp;Giovanna Rosendo Pereira ,&nbsp;Luana Czlusniak Godoy ,&nbsp;Maria Eduarda Martins Gomes ,&nbsp;Victoria Camargo Cachoeira ,&nbsp;Marcelo de Paula Loureiro ,&nbsp;Thais Andrade Costa Casagrande ,&nbsp;Gustavo Henrique Loesch ,&nbsp;Maíra de Mayo Oliveira Nogueira Loesch ,&nbsp;Felipe Francisco Tuon","doi":"10.1016/j.burns.2025.107522","DOIUrl":"10.1016/j.burns.2025.107522","url":null,"abstract":"<div><h3>Purpose</h3><div>Thermal burns are complex injuries that significantly impact morbidity and mortality. This study aimed to evaluate the effectiveness of biomaterials, including collagen gel (CG), collagen sponge (CS), non-decellularized amniotic membrane (NAM), and decellularized amniotic membrane (DAM), in promoting wound healing in a rat burn model.</div></div><div><h3>Methods</h3><div>Sixty Wistar rats were divided into five groups: control, CG, CS, NAM, and DAM. Burn wounds were induced using a heated metal rod, and treatments were applied accordingly. Histological and macroscopic analyses were conducted on days 7 and 14 post-burn to assess inflammation, proliferation of collagen fibers, angiogenesis, and re-epithelialization. Statistical comparisons of wound healing parameters were performed.</div></div><div><h3>Results</h3><div>By day 7, the CS group showed significant fibroblast proliferation and collagen deposition, facilitating early-stage healing. However, the NAM group demonstrated superior angiogenesis and granulation tissue formation by day 14, with advanced dermal matrix organization and nearly complete epithelialization, including hair regrowth. DAM exhibited moderate proliferation of collagen fibers and angiogenesis but lagged NAM in epidermal regeneration. CG promoted re-epithelialization and vascular remodeling but was less effective than NAM. The control group showed delayed healing with limited repair markers. Overall, NAM was the most effective biomaterial, followed by CG, CS, and DAM.</div></div><div><h3>Conclusion</h3><div>NAM emerged as the optimal treatment for burn healing, demonstrating superior efficacy in promoting angiogenesis, collagen organization, and epithelial regeneration. These findings underscore the potential of biological membranes in improving burn outcomes. Further human studies are warranted to validate these results and explore clinical applications.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 6","pages":"Article 107522"},"PeriodicalIF":3.2,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-precision computer-assisted surface area estimation in large surface burn patients using ImageJ – A comparative analysis","authors":"Florian Bucher,&nbsp;Nadjib Dastagir,&nbsp;Martynas Tamulevicius,&nbsp;Doha Obed,&nbsp;Thorben Dieck,&nbsp;Peter M. Vogt,&nbsp;Khaled Dastagir","doi":"10.1016/j.burns.2025.107524","DOIUrl":"10.1016/j.burns.2025.107524","url":null,"abstract":"<div><h3>Aims</h3><div>Several methods for calculating the extent of large surface burns have been described in the literature. However, the commonly used methods have several limitations and an overall tendency to overestimate the burn area. This study aimed to use ImageJ for high-precision computer-assisted estimation of large surface burns.</div></div><div><h3>Methods</h3><div>A retrospective analysis of the burn registry of the Department of Plastic, Aesthetic, Hand and Reconstructive Surgery of Hannover Medical School was performed from 1st January 2018 to 1st July 2024. Photo documentation of large surface burn patients was used to estimate the total body surface area (TBSA) using ImageJ and compared it to the estimations made by the referring emergency doctor, consultant plastic surgeon and a smartphone application (E-burn 1.0.0).</div></div><div><h3>Results</h3><div>A total of 37 patients were included in this study. The mean TBSA evaluated by ImageJ was 36.81 %, which was statistically significantly lower than estimations made by a consultant plastic surgeon with 41 % mean TBSA (p = 0.008) and referring emergency doctors with 50.97 % mean TBSA (p &lt; 0.0001). There was no statistically significant correlation between the estimation made by ImageJ and that made by the smartphone application which estimated a mean TBSA of 37.84 % (p = 0.1225).</div></div><div><h3>Conclusions</h3><div>ImageJ may be used for high-precision computer-assisted volume estimation of large surface burns by counting pixel by pixel to provide highly accurate TBSA estimations.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 5","pages":"Article 107524"},"PeriodicalIF":3.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor regarding “Association between platelet-to-lymphocyte ratio at admission and short-term mortality in severe burn patients: A meta-analysis”","authors":"Huishui Dai,&nbsp;Qifang Shi","doi":"10.1016/j.burns.2025.107520","DOIUrl":"10.1016/j.burns.2025.107520","url":null,"abstract":"","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 5","pages":"Article 107520"},"PeriodicalIF":3.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of early administration of inhaled heparin on outcomes of smoke inhalation injury: A randomized controlled trial","authors":"Sameh M. Hakim,&nbsp;Mariam N. Fouad,&nbsp;Mariam K. Habib,&nbsp;Mahmoud S. Mohamed,&nbsp;Safaa I. Ghaly","doi":"10.1016/j.burns.2025.107518","DOIUrl":"10.1016/j.burns.2025.107518","url":null,"abstract":"<div><h3>Background</h3><div>The present study aimed to examine the effects of early administration of inhaled heparin on the outcome of smoke inhalation injury.</div></div><div><h3>Methods</h3><div>Eighty-eight adults suffering smoke inhalation injury within 24 h were randomized to receive 5000 IU of heparin (n = 44) or normal saline (n = 44) by nebulization every 4 h until successful extubation or death, up to a maximum of 14 days. The primary outcome was ventilator-free days (VFDs) and alive at 28 days. The secondary outcomes included the intensive care unit (ICU)-free days and alive at 28 days, change in the PaO₂/FiO₂ ratio, 28-day all-cause mortality rate, and mechanical ventilation days in survivors.</div></div><div><h3>Results</h3><div>When adjusted to the burn area and burn-to-randomization time, inhaled heparin was associated with more VFDs (<em>P</em> =.046) and a higher cumulative incidence of weaning from mechanical ventilation over time (<em>P</em> =.007). Patients receiving inhaled heparin had more ICU-free days (<em>P</em> =.015), higher PaO₂/FiO₂ ratio (<em>P</em> =.001), and fewer mechanical ventilation days in survivors (<em>P</em> &lt;.001), but the mortality rate was comparable to the control group (<em>P</em> =.596).</div></div><div><h3>Conclusions</h3><div>The early administration of inhaled heparin to patients suffering smoke inhalation injury was associated with more VFDs and enhanced weaning from mechanical ventilation. Inhaled heparin was also associated with more ICU-free days, higher PaO₂/FiO₂ ratios, and fewer mechanical ventilation days in survivors. Larger randomized controlled trials are required to establish the role of inhaled heparin as a standard of care in this clinical setting.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 5","pages":"Article 107518"},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of DLAT in cuproptosis and autophagy in hippocampal tissue of PTSD rats with high-voltage electrical burns","authors":"Chenyang Ge,&nbsp;Xuegang Zhao,&nbsp;Jing Zhang,&nbsp;Peixuan Li,&nbsp;Jiawen Hao,&nbsp;Mengyuan Lu,&nbsp;Congying Li,&nbsp;Lili Ge,&nbsp;Lihong Tu,&nbsp;Qingfu Zhang","doi":"10.1016/j.burns.2025.107519","DOIUrl":"10.1016/j.burns.2025.107519","url":null,"abstract":"<div><h3>Background</h3><div>The mechanisms behind high-voltage electrical burn (HVEB)-induced post-traumatic stress disorder (PTSD) remain unclear. Accordingly, we aimed to identify the molecular changes in hippocampal tissue following HVEB-induced PTSD.</div></div><div><h3>Methods</h3><div>The GSE60303 dataset was used to identify differentially expressed cuproptosis-related genes (DE-CRGs) and to perform weighted gene co-expression network analysis. A core gene and associated genes were identified, followed by enrichment analysis. Additionally, a rat model of HVEB PTSD was established, and behavioral tests were conducted. Histological assessments and the evaluation of related protein and gene expression levels were performed on hippocampal tissue.</div></div><div><h3>Results</h3><div>Twelve DE-CRGs were identified in the hippocampal tissue of PTSD rats, with DLAT identified as the core gene. Analysis of DLAT-associated genes revealed enrichment in the cAMP signaling pathway and autophagy. Behavioral tests confirmed that HVEB induced PTSD-like behavior in rats. DLAT expression was decreased in the hippocampal tissue of HVEB PTSD rats, accompanied by changes in the expression of cuproptosis, cAMP pathway, and autophagy-related genes.</div></div><div><h3>Conclusion</h3><div>DLAT is reduced in the hippocampal tissue of HVEB PTSD rats. The downregulation of DLAT may contribute to the development of PTSD-like behaviors in HVEB rats by promoting cuproptosis, activating the cAMP pathway, and enhancing autophagy.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 5","pages":"Article 107519"},"PeriodicalIF":3.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and perspectives in understanding the role of CD147 in keloids","authors":"Hui Sun ,&nbsp;Wei Shen ,&nbsp;Ying-Ming Ma ,&nbsp;Hui-Qiong Nie ,&nbsp;Hua-Jie Zhong","doi":"10.1016/j.burns.2025.107517","DOIUrl":"10.1016/j.burns.2025.107517","url":null,"abstract":"","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 5","pages":"Article 107517"},"PeriodicalIF":3.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain stimulation methods for the management of chronic burn pain: A promising yet underexplored avenue","authors":"Seyed Javad Hosseini,&nbsp;Seyed Reza Hosseini,&nbsp;Mahbobeh Firooz","doi":"10.1016/j.burns.2025.107516","DOIUrl":"10.1016/j.burns.2025.107516","url":null,"abstract":"","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 5","pages":"Article 107516"},"PeriodicalIF":3.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}