Burns最新文献

{"title":"3D printed transparent face mask for burn scars management: French experience","authors":"Valerie Chauvineau, Valerie Joly, Emilie Hardy, Marie-Noelle Bartholomei","doi":"10.1016/j.burns.2025.107591","DOIUrl":"10.1016/j.burns.2025.107591","url":null,"abstract":"","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 7","pages":"Article 107591"},"PeriodicalIF":3.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nucleotide-binding domain-like receptor protein 3 inflammasome contributes to vascular endothelial barrier dysfunction and shock after burn injury","authors":"Huilin Yu ,&nbsp;Fengjie Lyu ,&nbsp;Zhe Cheng ,&nbsp;Minghao Luo ,&nbsp;Bryan Richard Sasmita ,&nbsp;Yuan Lan ,&nbsp;Dingyi Lv","doi":"10.1016/j.burns.2025.107589","DOIUrl":"10.1016/j.burns.2025.107589","url":null,"abstract":"<div><h3>Objective</h3><div>Burn injury-induced systemic inflammation significantly contributes to vascular endothelial dysfunction and hypotension. This study aimed to investigate the role of the nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasome in vascular endothelial barrier dysfunction following burn injury.</div></div><div><h3>Methods</h3><div>A 30 % total body surface area burn mouse model was established, and MCC950, a specific NLRP3 inhibitor, was administered (10 mg/kg) before and after burn injury. Serum collected from burned rats (burn serum, BS) at one day post-injury was applied to human acute mononuclear leukemia cells (THP-1) and human umbilical vein endothelial cells (HUVECs), with or without 10 μM MCC950<em>.</em> Blood pressure, 72-hour survival rates, serum levels of inflammatory cytokines (IL-1β and IL-18) and L-lactic acid (L-LA), and Evans blue staining of mesenteric arteries were measured. Protein expression of endothelial junction protein (VE-cadherin, ZO-1, occludin), MMP-9, NLRP3 inflammasome components (NLRP3, ASC, cleaved caspase-1), and pyroptosis executioner protein (N-terminal fragment of the gasdermin D protein, GSDMD-N) were examined both <em>in vivo</em> and <em>in vitro</em>. Cell viability and morphological changes of HUVECs, and inflammatory cytokines concentrations in THP-1 cell supernatants were also assessed.</div></div><div><h3>Results</h3><div>Burn injury significantly enhanced Evans blue extravasation in mesenteric arteries, and increased serum inflammatory cytokine and L-LA levels, accompanied by reduced blood pressure and decreased 72-hour survival rates <em>in vivo</em>. Burn injury or BS exposure led to decreased endothelial junction protein expression and increased expression of MMP-9, NLRP3 inflammasome components, and GSDMD-N both <em>in vivo</em> and <em>in vitro</em>. Furthermore, BS exposure resulted in reduced cell viability and evident pyroptotic morphology in HUVECs, along with elevated inflammatory cytokines in both the supernatants and lysates of THP-1 cells. Treatment with MCC950 effectively reversed all observed changes except for the protein expression of NLRP3 itself.</div></div><div><h3>Conclusion</h3><div>In burn injury, activation of the NLRP3 inflammasome is associated with enhanced inflammatory responses and endothelial cell pyroptosis, which may contribute to endothelial barrier dysfunction and the progression of shock.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 7","pages":"Article 107589"},"PeriodicalIF":3.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of music therapy on background pain, anxiety, depression, vital signs, and medication usage in adult burn patients in the Intensive Care Unit: A randomized controlled trial","authors":"Mark Ettenberger ,&nbsp;Rafael Maya ,&nbsp;Andrés Salgado-Vasco ,&nbsp;Jose Gabriel Cordoba-Silva ,&nbsp;Moshé Amarillo ,&nbsp;William Betancourt-Zapata ,&nbsp;Juliana Marín-Sánchez ,&nbsp;Viviana Gómez-Ortega ,&nbsp;Mario Valderrama","doi":"10.1016/j.burns.2025.107587","DOIUrl":"10.1016/j.burns.2025.107587","url":null,"abstract":"<div><h3>Introduction</h3><div>Burn patients often experience pain and mental health difficulties in the Intensive Care Unit (ICU). This study investigated the effects of live Music-Assisted Relaxation (MAR) on background pain, mental health, vital signs, and medication administration in adult ICU burn patients.</div></div><div><h3>Method</h3><div>This was a randomized controlled trial with two parallel arms: standard care + up to six MAR interventions over two weeks, versus standard care alone. Participants were 82 adult ICU burn patients. Primary outcome was perceived background pain. Secondary outcomes were anxiety and depression levels, vital signs, and pain medication usage. Additionally, nine patients participated in electroencephalography, electrocardiography, and electromyography recordings during MAR.</div></div><div><h3>Results</h3><div>Results showed that background pain was significantly lower in the intervention group than in the control group after MAR sessions 1 and 2, with a large effect for patients reporting severe pain. Time had a significant effect on both anxiety and depression, but this was unrelated to the treatment condition. No differences were found for vital signs or pain medication administration. Analysis of electrophysiological signals during MAR revealed significant changes in delta, theta, beta, and alpha frequency bands during electroencephalography, a significant decrease of the low/high-frequency ratio of the tachogram, and significant decrease of mean frequency of facial electromyography.</div></div><div><h3>Conclusions</h3><div>The results suggest that music therapy might be effective in reducing background pain during the early stages of hospitalization and specifically for patients with severe pain. Further multicenter studies with a larger sample size are needed to confirm these results. Trial registration: <span><span>www.clinicaltrials.gov</span><svg><path></path></svg></span>, Identifier: NCT04571255.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 7","pages":"Article 107587"},"PeriodicalIF":3.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of punch excision in the treatment of severe burn scars","authors":"Xin-Xin Chen,&nbsp;Zhe Yin,&nbsp;Duo Cai,&nbsp;Tai-yuan Ma,&nbsp;Xiu-Hang Zhang,&nbsp;Yu-Tang Li,&nbsp;Yi-Yi He,&nbsp;Qing-Hua Yu,&nbsp;Jia-Ao Yu","doi":"10.1016/j.burns.2025.107578","DOIUrl":"10.1016/j.burns.2025.107578","url":null,"abstract":"<div><h3>Introduction</h3><div>This study aims to identify the therapeutic outcomes of punch excision in severe burn patients with extensive, excessive and long-lasting growth scars.</div></div><div><h3>Methods</h3><div>A retrospective comparative clinical observational study was conducted on patients with burn hypertrophic scars who were treated at the Department of Burn and Skin Wound Repair, First Hospital of Jilin University, between October 2022 and October 2024.</div></div><div><h3>Results</h3><div>A total of 49 patients with severe burn scars were included. Of these, 20 received combined treatment with ablative fractional carbon dioxide laser (AFCL) and punch excision (PE), while 29 were treated with AFCL alone. The AFCL+PE group showed faster and more significant improvements in scar height, pliability, and paresthesias. At 1 week post-treatment, the University of North Carolina 4P Scar Scale (UNC4P) pliability score decreased from 2.7 ± 0.5–2.2 ± 0.6, and the paresthesias score improved from 2.5 ± 0.7–1.9 ± 0.9. The Vancouver Scar Scale (VSS) pliability score dropped from 4.1 ± 0.8–3.4 ± 0.9, and the height score improved from 3.0 ± 0.0–2.1 ± 0.6. Significant improvements in scar sensation were noted, with the Semmes-Weinstein monofilament test decreasing from 32.9 ± 27.2 g pre-treatment to 15.0 ± 10.3 g at 1 week, further improving to 9.5 ± 7.7 g at 1 month and 8.4 ± 7.6 g at 3 months. All 20 patients tolerated the AFCL+PE treatment well, with no complications. The average healing time for PE was 11.7 ± 2.2 days. Postoperative adverse effects included pigmentation (n = 5), which appeared 7 days post-treatment, and mild itching (n = 1).</div></div><div><h3>Conclusion</h3><div>PE is a safe and effective method for scar management, with excellent suitability for hypertrophic burn scars. It can achieve significant rates of scar volume reduction, rapidly and extensively improve scar thickness, hardness, and scar sensation. As an emerging treatment, punch excision offers a distinct therapeutic mechanism and indications compared to existing methods. It holds the potential to become an integral part of current multi-modal scar management approaches. In the future, scar thickness and hardness may no longer be the primary concerns for patients with scars.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 7","pages":"Article 107578"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does trauma center designation impact management, complications, and outcomes in burn patients? A National Trauma Data Bank analysis","authors":"Eloise W. Stanton ,&nbsp;Artur Manasyan ,&nbsp;Sean Donohue ,&nbsp;Justin Gillenwater ,&nbsp;Mathew Martin ,&nbsp;Kenji Inaba ,&nbsp;Haig A. Yenikomshian","doi":"10.1016/j.burns.2025.107588","DOIUrl":"10.1016/j.burns.2025.107588","url":null,"abstract":"<div><h3>Background</h3><div>Burn injuries, particularly those involving large total body surface area (TBSA), present significant challenges due to fluid shifts, increased metabolic demands, and heightened susceptibility to complications. Trauma center designation, ranging from Level 1 (highest acuity) to Level 3, reflects differences in resources, expertise, and care protocols, which may influence patient outcomes. This study evaluates the impact of trauma center designation on transfusion practices, complications, and mortality among burn patients.</div></div><div><h3>Methods</h3><div>The Trauma Quality Improvement Program (TQIP) database (2017–2022) was queried to identify burn patients by ICD-9/10 codes. Patients were categorized based on trauma center designation: Level 1 versus non-Level 1 (Levels 2 and 3). Outcomes analyzed included transfusion practices (e.g., packed red blood cells, plasma, platelets), inpatient complications, and mortality. Multivariable logistic regression was performed, adjusted for age, gender, %TBSA, inhalation injury, and Injury Severity Score (ISS). Subgroup analysis was performed for patients with burns &gt; 20 % TBSA.</div></div><div><h3>Results</h3><div>Among 72,474 burn patients, 47 % were treated at Level 1 centers. Patients at Level 1 centers presented with higher injury severity and had significantly higher rates of complications, including respiratory failure, sepsis, and surgical site infections. Despite the higher complication rates, Level 1 centers demonstrated significantly lower mortality compared to non-Level 1 centers (adjusted OR=0.46, 95 % CI 0.25–0.83, p = 0.011). Transfusion practices did not differ significantly between center levels after adjustment. In patients with burns &gt; 20 % TBSA, Level 1 centers maintained lower mortality rates (adjusted OR=0.83, 95 % CI 0.79–0.88, p &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>Trauma center designation is associated with significant differences in burn outcomes. Level 1 centers, despite managing more severe cases, demonstrate lower mortality, emphasizing the importance of specialized care for burn patients. Standardizing burn care protocols and optimizing transfer practices may further improve outcomes for this high-acuity population.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 7","pages":"Article 107588"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive value of veno-arterial carbon dioxide partial pressure difference and central venous oxygen saturation for fluid administration and mortality in critically ill patients with extensive burns","authors":"Benedikt F. Scherr ,&nbsp;Pedro D. Wendel-Garcia ,&nbsp;Hannes Bruns ,&nbsp;Peter Steiger ,&nbsp;Reto A. Schuepbach ,&nbsp;Bong-Sung Kim ,&nbsp;Mauro Vasella ,&nbsp;Giovanni Camen ,&nbsp;Philipp Karl Buehler","doi":"10.1016/j.burns.2025.107585","DOIUrl":"10.1016/j.burns.2025.107585","url":null,"abstract":"<div><h3>Objectives</h3><div>Guiding fluid therapy in severely burned patients presents complex challenges, combining elements of both distributive and hypovolemic shock, accompanied by cardiovascular dysfunction. Whereas parts of hemodynamic assessment in septic shock such as central venous oxygen saturation (ScvO2) and the arterial-venous carbon dioxide (CO2) partial pressure difference (ΔpCO2 = pcvCO2 - paCO2) can both be used as indicators of hypoperfusion, they are not part of the standard clinical assessment of severely burned patients. The aim of this retrospective study was thus to investigate whether the ΔpCO2 and ScvO2 in severely burned patients correlates with fluid administration and mortality.</div></div><div><h3>Methods</h3><div>Retrospective analysis of severely burned patients with burns larger than 20 % total body surface area (TBSA) admitted between 01/2017 and 06/2021 to the Burns Center of the University Hospital Zurich, Switzerland. Patients were treated according to international guidelines and in-house standards and ΔpCO2 and ScvO2 were assessed at least once within the first 24 h of intensive care unit admission.</div></div><div><h3>Results</h3><div>In total 69 patients were included in this analysis. The median ΔpCO2 and ScvO2 were 1.16 kPa [inter-quartile range IQR, 0.82 – 1.50] and 76 % [IQR, 71 – 81]. This translated to an abnormally elevated ΔpCO2 (&gt;0.8 kPa) in 53 (77 %) and an abnormally reduced ScvO2 (&lt;70 %) in 13 (19 %) patients. Initial ΔpCO2, but not ScvO2, was non-linearly associated with intravenous fluid administration in the following 24 h (estimate_log 9.6 L [95 % confidence interval CI, 4.1 – 15.2], p = 0.001) and 48 h (estimate_log 6.0 L [95 % CI, 0.3 – 11.8], p = 0.039). Both initial ΔpCO2 (logarithmic odds ratio OR_log 4.3 [95 % CI, 1.1 – 20.9], p = 0.048) as well as ScvO2 (OR_log 0.1 [95 % CI, 0 – 0.42], p = 0.034) were non-linearly associated with in-hospital mortality. However, the best association with mortality was achieved by combining ΔpCO2 and ScvO2, with patients presenting with abnormalities in both ΔpCO2 and ScvO2 having a predicted mortality of 46 % [95 % CI, 23–71].</div></div><div><h3>Conclusion</h3><div>In severely burned patients, both ΔpCO2 and ScvO2 are associated with the extent and severity of burns as well as with in-hospital mortality. However, only ΔpCO2 seems to possess potential as a predictor of fluid administration. A multimodal approach to fluid resuscitation including both parameters may show promise in severely burned patients; however, further prospective studies are required to define optimal thresholds and validate its integration into clinical fluid resuscitation practice.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 8","pages":"Article 107585"},"PeriodicalIF":3.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Hypoxia impairs autophagy of cardiomyocytes via p38/MAPK/MAP4 pathway” [Burns, Vol. 51 (2025) 107511]","authors":"Nuo Chen ,&nbsp;Qiongfang Ruan ,&nbsp;Siyu Zhang ,&nbsp;Zhigang Chu ,&nbsp;Weiguo Xie","doi":"10.1016/j.burns.2025.107564","DOIUrl":"10.1016/j.burns.2025.107564","url":null,"abstract":"","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 6","pages":"Article 107564"},"PeriodicalIF":3.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asiatic acid inhibits keloid fibroblast migration and collagen deposition via suppression of STAT3 activation","authors":"Jiaqi Wu ,&nbsp;Guizhen Xu ,&nbsp;Xinlu Zhou,&nbsp;Jun Wan,&nbsp;Huaiwei Liao,&nbsp;Cong Li,&nbsp;Yan Shi,&nbsp;Huicai Wen","doi":"10.1016/j.burns.2025.107586","DOIUrl":"10.1016/j.burns.2025.107586","url":null,"abstract":"<div><h3>Background</h3><div>This study investigated the pharmacological effects of asiatic acid (AA) on keloid fibroblasts (KFs) and elucidated its therapeutic mechanisms in keloid pathogenesis.</div></div><div><h3>Methods</h3><div>Primary human KFs were isolated and expanded for experimental analysis. Cell migration capacity was evaluated through cell scratch assay and Boyden chamber assay. Immunofluorescence staining was performed to quantify α-smooth muscle actin (α-SMA) expression in AA-treated KFs, while Western blot assessed collagen type I (Col-I), α-SMA, STAT3, and phosphorylated STAT3 (p-STAT3) protein levels. STAT3 activation was pharmacologically induced in KFs using Colivelin TFA (a STAT3 agonist), with subsequent evaluation of migratory behavior and protein expression profiles using parallel methodologies.</div></div><div><h3>Results</h3><div>AA treatment significantly inhibited the viability and migratory capacity of KFs, accompanied by downregulation of Col-I, α-SMA, and p-STAT3 expression. Pharmacological activation of STAT3 via Colivelin TFA partially rescued AA-induced suppression of KF migration and Col-I expression.</div></div><div><h3>Conclusions</h3><div>AA significantly modulates the migratory capacity and extracellular matrix (ECM)-related protein expression in KFs, with the STAT3 signaling pathway implicated in this regulatory mechanism. These findings indicate that the anti-fibrotic effects of AA are mediated through STAT3 signaling.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 8","pages":"Article 107586"},"PeriodicalIF":3.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for antimicrobial resistance in paediatric burn infections: Insights from a retrospective cohort study","authors":"Anteneh Amsalu ,&nbsp;Anton Alvaro ,&nbsp;Steven Huang ,&nbsp;Andrew May ,&nbsp;Anna Antipov ,&nbsp;Linda Quinn ,&nbsp;Bernard Carney ,&nbsp;Zlatko Kopecki","doi":"10.1016/j.burns.2025.107584","DOIUrl":"10.1016/j.burns.2025.107584","url":null,"abstract":"<div><h3>Aim</h3><div>To define the microbiome, antimicrobial resistance profiles and associated risk factors among paediatric patients with infected burns.</div></div><div><h3>Methods</h3><div>A retrospective cohort study was conducted among paediatric patients with infected burns admitted to a tertiary burns service between January 2011 to December 2023. Basic demographic data and burn-related clinical information were extracted from the Burns Unit database and linked with microbiological data.</div></div><div><h3>Result</h3><div>Among a total of 3679 paediatric burn patients admitted, 183 (5 % of overall admitted) were identified as clinically having infected burns. Of the 173 (4.7 % of overall admitted) patients with documented cultures, 152 (87.9 % of suspected clinical infections) had culture-positive burn wound infections (BWIs) and 15 (8.7 % of overall admitted) had developed blood stream infections. The most common microorganisms identified in BWI were Gram-positive bacteria (245 isolates, 63.1 %), with <em>Staphylococcus aureus</em> being the most prevalent (32 %) followed by <em>Streptococcus</em> species (11.9 %). Gram-negative bacteria were identified in 32.5 % of cases, with <em>Pseudomonas aeruginosa</em> being the most common organism (5.7 %). Nineteen (5 %) methicillin-resistant <em>Staphylococcus aureus</em> isolates were detected from 17 (9.8 %) paediatric patients with burns. The highest resistance was reported against ampicillin (100 %) followed by penicillin (91.7 %), and amoxicillin (88.6 %) against <em>S. aureus</em> isolates. <em>P. aeruginosa</em> isolates showed resistance in 58.8 % of cases to ceftazidime, followed by 47 % to piperacillin-tazobactam, and 2 isolates were resistant to imipenem, a carbapenem antibiotic considered a last-resort option. Multivariate logistic regression analysis revealed that burns to the head and neck regions (AOR = 5.2, 95 %CI: 2.20–12.31; <em>p</em> <em>&lt;</em> 0.001), admission to the paediatric intensive care unit (PICU) (AOR = 8.2, 95 %CI: 1.03–64.86; <em>p</em> = 0.047) and previous medical history (AOR = 2.4, 95 %CI: 1.07–5.55; <em>p</em> = 0.033) were independent risk factors associated with antimicrobial-resistant (AMR) burn infections</div></div><div><h3>Conclusions</h3><div>AMR in paediatric patients with infected burns is common and therefore early culture confirmation could improve treatment outcomes especially for patients with high risk factors.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 6","pages":"Article 107584"},"PeriodicalIF":3.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Red propolis cream and its therapeutic potential for skin lesions caused by burns","authors":"Lauriene Luiza de Souza Munhoz ,&nbsp;Jennifer Jullie Pichinelli Noronha ,&nbsp;Patrícia Reginato Faccioti ,&nbsp;Caio Garcia Barbosa da Silva ,&nbsp;Samuel Henrique Gomes de Sá ,&nbsp;Severino Matias Alencar ,&nbsp;Carlos Eduardo Ambrósio ,&nbsp;Carmen Sílvia Favaro-Trindade ,&nbsp;Daniele dos Santos Martins","doi":"10.1016/j.burns.2025.107583","DOIUrl":"10.1016/j.burns.2025.107583","url":null,"abstract":"<div><div>Skin lesions, particularly burns, compromise the skin barrier and require effective treatment to accelerate healing and prevent infections. This study evaluated a topical formulation containing Olivem® 1000 and red propolis, which is rich in flavonoids, phenolic acids, and other bioactive compounds with antioxidant, antimicrobial, and anti-inflammatory properties. Stability tests confirmed that the formulation remained stable under high temperatures, with minimal changes in pH and viscosity, ensuring long-term effectiveness. The microbiological analysis demonstrated potent antimicrobial activity, with no microbial growth detected after 28 days against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>. <em>In vitro</em> assays on L929 cells revealed a concentration-dependent effect on viability, with cytotoxicity observed above 40 µg/mL (IC₅₀). The scratch assay demonstrated enhanced cell migration, indicating a positive effect on wound closure, while fluorescence microscopy confirmed increased cell proliferation and superoxide dismutase (SOD) levels, suggesting antioxidant protection. In addition, the formulation showed a marked reduction in the expression of pro-inflammatories, such as IL-6 and TNF-α. <em>In vivo</em>, treatment of second-degree burns in Wistar rats with Olivem®1000 and red propolis accelerated wound closure, reduced crust formation, and promoted more organized tissue compared to untreated controls. By day 21, nearly complete regeneration was observed, with a continuous epidermis and increased collagen deposition. Histological analysis revealed enhanced extracellular matrix organization. These findings underscore the formulation’s capacity to enhance tissue repair, improve wound quality, thereby suggesting its potential as a preclinical candidate for further studies in the domain of burn treatment.</div></div>","PeriodicalId":50717,"journal":{"name":"Burns","volume":"51 8","pages":"Article 107583"},"PeriodicalIF":3.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}