{"title":"Induction of direct organogenesis from aerial explants of scented alstroemeria genotypes","authors":"C. Guzmán, C. Rivas, L. Prat, D. Aros","doi":"10.7764/RCIA.V45I2.1918","DOIUrl":"https://doi.org/10.7764/RCIA.V45I2.1918","url":null,"abstract":"Alstroemeria is an important pot plant and cut flower in the ornamental plant market, and its propagation mainly occurs by rhizome division. However, this method is inefficient and timeconsuming and may contribute to the spread of viruses. This study focused on developing in vitro propagation protocols using aerial explants of Alstroemeria caryophyllaea and A. ‘Sweet Laura’, two scented genotypes. Aerial explants of alstroemeria were regenerated by testing three types of aerial explants and four concentrations of cytokinins. Both A. caryophyllaea and A. ‘Sweet Laura’ showed the highest induction rate from shoots, with values of 35 and 63.3%, respectively. For both genotypes, the optimum BAP concentration for direct organogenesis from buds was 1.0 mg L-1. Finally, a successful protocol for in vitro propagation of A. caryophyllaea and A. ‘Sweet Laura’ through direct organogenesis from aerial explants was generated.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":"69 1","pages":"158-168"},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89607321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Pizarro-Aránguiz, D. García-Mendoza, Ruben Muñoz-Obregon, B. S. Núñez, Rodrigo Pavez
{"title":"Impact of environmental variables on PCDD/F and dl-PCB levels in dairy milk of the farming region of Chile","authors":"N. Pizarro-Aránguiz, D. García-Mendoza, Ruben Muñoz-Obregon, B. S. Núñez, Rodrigo Pavez","doi":"10.7764/RCIA.V45I2.1887","DOIUrl":"https://doi.org/10.7764/RCIA.V45I2.1887","url":null,"abstract":"According to a One Health perspective, the importance of persistent organic pollutants (POPs) must be assessed because of their impact on the environment, food chain and human health. However, information on these toxic compounds is limited in Latin America and the Caribbean region. Chile is no exception; therefore, this work aimed to explain previously reported dioxin levels in cow-milk samples by utilizing regression with meteorological/geographical data that were collected over a three-year survey. To accomplish this aim, a stepwise general multiple regression analysis was carried out for polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and dioxin-like polychlorinated biphenyls (dl-PCBs). The best statistical adjustments were achieved only for highly present congeners. Regarding PCDD/F congeners, the most relevant and significant (P <0.05) factors were the year (mostly a negative coefficient), the season, and the hectares affected by forest fires. In the case of dl-PCB congeners, there was a clear, positive relationship with the geographic parameter (UTM), and this result was consistent with previous findings that dl-PCB congeners show a trend with latitude. In contrast, wind speed was a significant negative coefficient for dl-PCBs. Despite existing knowledge on pollutant levels in milk, this study is relevant to better understand these findings in the Latin America and Caribbean regions.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":"44 1","pages":"109-119"},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86396833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chemical Composition and Antifungal and Antibacterial Activity of Mentha spicata L. Volatile Oil","authors":"Y. Bayan, M. Küsek","doi":"10.7764/RCIA.V45I1.1897","DOIUrl":"https://doi.org/10.7764/RCIA.V45I1.1897","url":null,"abstract":"In this study, we researched the chemical composition and the antifungal and antibacterial activity of volatile oil from Mentha spicata . The Gas chromatography/mass spectrometry (GC/MS) analysis of M. spicata showed that the main component was carvone (56.94%), followed by limonene (11.63%), sabinene hydrate (7.04%) and caryophyllene (4.06%). The antifungal activity of the volatile oil from M. spicata L. was determined with respect to plant pathogenic fungi, such as Fusarium oxysporum f. sp. radicis-lycopersici (Sacc.) W.C. Synder & H.N. Hans (FORL), Rhizoctonia solani J.G. Kuhn. (R. solani), Alternaria solani (A. solani), and Verticillium dahliae Kleb (V. dahliae). The volatile oil was shown to have strong antifungal activity against plant pathogenic fungi. The result of the study was that at a dose of 12 μL petri-1, the volatile oil inhibited 100% of mycelium growth in V. dahliae, A. solani FORL and R. solani . Volatile oil exhibited remarkable activity against the selected bacterial strains of Xanthomonas spp. (ZI365, ZI366, ZI368, ZI370, ZI373, ZI375, ZI376, ZI378).","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":"114 ","pages":"64-69"},"PeriodicalIF":0.0,"publicationDate":"2018-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.7764/RCIA.V45I1.1897","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72446260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Felipe Ponce, Yaneris Mirabal-Gallardo, A. Versari, V. F. Laurie
{"title":"The use of cation exchange resins in wines: Effects on pH, tartrate stability, and metal content","authors":"Felipe Ponce, Yaneris Mirabal-Gallardo, A. Versari, V. F. Laurie","doi":"10.7764/RCIA.V45I1.1911","DOIUrl":"https://doi.org/10.7764/RCIA.V45I1.1911","url":null,"abstract":"Treating wines with cation exchange resins allows the reduction of pH and contributes to limiting the formation of tartrate salts by exchanging cations such as potassium with hydrogen ions. This manuscript summarizes the results of a series of laboratory and winery-scale trials performed with the aim of evaluating the ion exchange process and its effects on the chemical composition of the treated samples. The laboratory-scale results showed that both the procedure employed for the activation of resins and the chemical composition of the wines affected the extent of the chemical changes occurring during the treatment. As such, the winery-scale trials showed that the resin-treated wines have significantly lower pH, higher total acidity, less tartrate formation (measured by weight), and a reduced amount of most metals analyzed. Wine samples blended with approximately 20% of cation exchange-treated samples (by volume) showed no signs of tartrate instability when assessed by a quick qualitative cold test.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":"145 1","pages":"82-92"},"PeriodicalIF":0.0,"publicationDate":"2018-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82654188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Moser, E. Uhl, T. Rötzer, P. Biber, J. Caldentey, H. Pretzsch
{"title":"Effects of climate trends and drought events on urban tree growth in Santiago de Chile","authors":"A. Moser, E. Uhl, T. Rötzer, P. Biber, J. Caldentey, H. Pretzsch","doi":"10.7764/RCIA.V45I1.1793","DOIUrl":"https://doi.org/10.7764/RCIA.V45I1.1793","url":null,"abstract":"Urban trees and the services they provide (e.g., evapotranspirational cooling, shading, recreation, carbon storage, air pollution filtering) can have major effects on the microclimate of a city, although the growth conditions are often inadequate to ensure tree vitality and growth, negatively affecting their beneficial effects. In a worldwide dendrochronological study on ten urban tree species in four climatic zones, the growth and impacts of common urban tree species were assessed. This paper focuses on the results for Robinia pseudoacacia L. in the Mediterranean climate city of Santiago de Chile, highlighting the faster growth of the studied black locust trees since 1960 than its growth in the years before 1960. Furthermore, black locust displayed the best growth when situated closer to the city center than the city periphery and when in the northern and western parts of Santiago de Chile. The species characteristics of black locust also revealed an immediate negative growth reaction to drought events, followed by a rapid recovery, which was similarly influenced by the direction from and distance to the city center of the growing site. The results underline the overall worldwide findings on urban tree growth that indicate that a city climate with an extended growing season and increased temperatures can lead to improved growth of urban trees in the Mediterranean climatic zone. However, with increased growth, more rapid ageing and tree death might follow, leading to increased costs for new plantings and tree management.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":"27 1","pages":"35-50"},"PeriodicalIF":0.0,"publicationDate":"2018-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73287090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Alomar, P. Ruiz, L. Balocchi, G. Valenzuela, D. Goic
{"title":"Finishing lambs on a chicory-plantain mixture or a temperate grassbased pasture: live weight gain and gastrointestinal parasitism","authors":"C. Alomar, P. Ruiz, L. Balocchi, G. Valenzuela, D. Goic","doi":"10.7764/RCIA.V45I1.1874","DOIUrl":"https://doi.org/10.7764/RCIA.V45I1.1874","url":null,"abstract":"Improved plantain ( Plantago lanceolata L.) and chicory ( Cichorium intybus L.) are attractive forage species for finishing lambs and contain compounds that might protect sheep from gastrointestinal nematodes. This study compared the live weight gain and parasite burden of weaned lambs finished on a plantain-chicory mix (Pl-C) or a permanent grassbased pasture (PP) and treated with or without an endectocide. From mid-November, weaned lambs (n=72, average live weight 32 kg) were distributed into three paddocks per sward type for 30 d. Half of the lambs were initially treated with ivermectin. Fecal egg counts (FEC), larvae genera and the presence of infective larvae (L3) in pastures were evaluated. No differences (P>0.05) were obtained between the pastures for the initial (ca. 80 epg) or final FEC (322 epg) or type of eggs (Strongylidor Nematodirus-type). Ivermectin treatment reduced (P 0.05) of pasture type or anthelmintic treatment and no interactions were detected on the cultured larvae genera, except for Cooperia, which decreased with the anthelmintic treatment. By day 30, the number of L3 recovered from the pastures increased (P 0.05) in the Pl-C (average 63 L3 kg-1 DM). The parasite load estimated by the FEC reached low infection levels ( 0.05) among treatments (average 173 g d-1), reflecting an adequate nutritional quality of both pastures and a moderate parasitism status of the lambs.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":"59 2 1","pages":"11-20"},"PeriodicalIF":0.0,"publicationDate":"2018-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88215311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jorge Gutiérrez-Tlahque, C. Aguirre-Mancilla, J. C. Raya-Pérez, J. Ramírez-Pimentel, R. Jiménez-Alvarado, A. Hernández-Fuentes
{"title":"Effect of climate conditions on total phenolic content and antioxidant activity of Jatropha dioica Cerv. var. dioica","authors":"Jorge Gutiérrez-Tlahque, C. Aguirre-Mancilla, J. C. Raya-Pérez, J. Ramírez-Pimentel, R. Jiménez-Alvarado, A. Hernández-Fuentes","doi":"10.7764/RCIA.V45I1.1832","DOIUrl":"https://doi.org/10.7764/RCIA.V45I1.1832","url":null,"abstract":"The aim of this work was to determine the antioxidant activity, total phenolic content (TPC) and total flavonoid content (TFC) in the rhizomes and stems of Jatropha dioica and their relation to collection season, collection location, extraction solvent and their interactions to understand the effect of climate conditions on the synthesis of the antioxidant compounds in J. dioica . Plants were collected during different seasons at two locations in Mexico. A 3-factorial experimental design was used for the stems and rhizomes, using the extraction solvent, collection location and season as sources of variation. A Tukey’s test with a P≤0.05 significance level was used to perform the comparison of the means. Significant differences were found when comparing the three sources of variation separately for TPC and antioxidant activity in the stems and rhizomes. In terms of the interactions for the stems, there was a significant difference between the Morelos × 70% ethanol × dry interaction and Tetepango × water × rain interaction for the TPC and the antioxidant activity; however, no significant difference was found for the TFC. In terms of the rhizomes, a significant difference was found among the 4 variables analyzed. These results indicate that collection location, collection season and extraction solvent affect the TPC and antioxidant activity. In addition, the rhizomes presented higher antioxidant activity and TPC than that of the stems.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":"4 1","pages":"70-81"},"PeriodicalIF":0.0,"publicationDate":"2018-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74756405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Susceptibility of different grapevine (Vitis vinifera L.) cultivars to Diplodia seriata and Diplodia mutila","authors":"M. Ramírez, L. M. Pérez, J. Montealegre","doi":"10.7764/rcia.v45i1.1818","DOIUrl":"https://doi.org/10.7764/rcia.v45i1.1818","url":null,"abstract":"Canes obtained from one-year-old stems of the grapevine cultivars Cabernet Franc, Malbec, Merlot, Sauvignon Blanc and Syrah (all non-grafted) were used as models to test their susceptibility to Diplodia seriata and Diplodia mutila . The results showed that the mean length of lesions caused by D. mutila was 3.2 times larger than that produced by D. seriata . In addition, the Cabernet Franc and Syrah cultivars were the most susceptible to D. mutila , while Merlot and Malbec were the least susceptible. No significant differences were observed between cultivars inoculated with D. seriata . The results are discussed in terms of the susceptibility of the different grapevine cultivars to both pathogens.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":"11 1","pages":"93-98"},"PeriodicalIF":0.0,"publicationDate":"2018-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83665854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of daily herbage allowance restriction on pasture characteristics and milk production by grazing dairy cows in spring","authors":"V. Merino, L. Balocchi, F. Pulido","doi":"10.7764/RCIA.V45I1.1841","DOIUrl":"https://doi.org/10.7764/RCIA.V45I1.1841","url":null,"abstract":"The cumulative effects of daily herbage allowance (DHA) restriction on pasture characteristics and the sustainability of pasture-based dairy systems were evaluated. Sixty-four dairy cows were randomly assigned to one of two grazing treatments (20 and 30 kg DM cow-1 day-1). Milk yield and pre- and post-grazing herbage mass were recorded for every grazing event. Botanical and chemical compositions of the pasture were evaluated. Low DHA increased the stocking rate by 0.7 cows ha-1 and consequently increased grazing efficiency from 41 to 47%. Daily herbage allowance restriction did not affect pasture characteristics or milk production per cow, but DHA restriction increased milk production per hectare (+27%). The results suggest that restriction on daily herbage allowance ensures a higher level of herbage utilization and milk output per ha than high DHA, without affecting pasture characteristics. Therefore, DHA might be a useful grazing management tool for improving the profitability and sustainability of grazing-based dairy systems.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":"25 1","pages":"21-34"},"PeriodicalIF":0.0,"publicationDate":"2018-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83279168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Gallardo, Yeon A Hong, Marcial Ibo Silva Jaimes, Johanna Flores Orozco
{"title":"Investigating consumer food choice behavior: an application combining sensory evaluation and experimental auctions","authors":"R. Gallardo, Yeon A Hong, Marcial Ibo Silva Jaimes, Johanna Flores Orozco","doi":"10.7764/RCIA.V45I1.1765","DOIUrl":"https://doi.org/10.7764/RCIA.V45I1.1765","url":null,"abstract":"We investigate what piece of information collected utilizing sensory evaluation tools exhibits better predictive capacity on the willingness to pay, is it information from preferences for a sensory quality attribute using hedonic scales or information on perceived intensity for the same attribute using intensity scales? We also estimated if extrinsic or intrinsic quality exerts a similar impact on consumer’s willingness to pay. We conducted a sensory evaluation along with experimental auctions using three different apple varieties with college students in Metropolitan Lima, Peru. Findings from this study show that information collected on preference liking for apple quality attributes has a better explanatory capability for willingness to pay, compared to information on consumers’ perceived intensity for the same attribute. The explanatory capability was measured using measures of goodness-of-fit. We also prove that willingness to pay was driven both by the apple variety induced intrinsic quality attributes and the extrinsic cues of the variety. Results add to the existing body of literature aiming to improve the understanding of consumer food choice behavior.","PeriodicalId":50695,"journal":{"name":"Ciencia E Investigacion Agraria","volume":"116 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2018-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79744713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}