香花苜蓿基因型空中外植体直接器官发生的诱导

Q2 Agricultural and Biological Sciences

Ciencia E Investigacion Agraria Pub Date : 2018-08-30 DOI:10.7764/RCIA.V45I2.1918

C. Guzmán, C. Rivas, L. Prat, D. Aros

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引用次数: 1

摘要

桤木是观赏植物市场上重要的盆栽植物和切花，其繁殖主要通过根茎分裂进行。然而，这种方法效率低、耗时长，而且可能助长病毒的传播。本研究主要研究了两种有香味基因型石竹(Alstroemeria caryophyllaea)和香劳拉(A. ' Sweet Laura ')的离体繁殖方案。通过对三种类型的空中外植体和四种浓度的细胞分裂素的试验，再生了桤木的空中外植体。石竹和香劳拉的诱导率最高，分别为35%和63.3%。对于两个基因型，芽直接器官形成的最佳BAP浓度为1.0 mg L-1。最后，成功地建立了石竹和甜劳拉通过空中外植体直接器官发生的离体繁殖方案。

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Induction of direct organogenesis from aerial explants of scented alstroemeria genotypes

Alstroemeria is an important pot plant and cut flower in the ornamental plant market, and its propagation mainly occurs by rhizome division. However, this method is inefficient and timeconsuming and may contribute to the spread of viruses. This study focused on developing in vitro propagation protocols using aerial explants of Alstroemeria caryophyllaea and A. ‘Sweet Laura’, two scented genotypes. Aerial explants of alstroemeria were regenerated by testing three types of aerial explants and four concentrations of cytokinins. Both A. caryophyllaea and A. ‘Sweet Laura’ showed the highest induction rate from shoots, with values of 35 and 63.3%, respectively. For both genotypes, the optimum BAP concentration for direct organogenesis from buds was 1.0 mg L-1. Finally, a successful protocol for in vitro propagation of A. caryophyllaea and A. ‘Sweet Laura’ through direct organogenesis from aerial explants was generated.

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来源期刊

Ciencia E Investigacion Agraria 农林科学-农业综合

CiteScore

1.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The subject matter that is considered to be appropriate for publication in International Journal of Agriculture and Natural Resources (formerly Ciencia e Investigación Agraria) is all new scientific and technological research in agriculture, animal production, forestry, natural resources and other related fields.