CladisticsPub Date : 2022-07-04DOI: 10.1111/cla.12509
Andrew V. Z. Brower
{"title":"Intersubjective Corroboration","authors":"Andrew V. Z. Brower","doi":"10.1111/cla.12509","DOIUrl":"10.1111/cla.12509","url":null,"abstract":"","PeriodicalId":50688,"journal":{"name":"Cladistics","volume":"38 5","pages":"612-621"},"PeriodicalIF":3.6,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46511747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"New insights into the phylogeny of the complex thalloid liverworts (Marchantiopsida) based on chloroplast genomes","authors":"You-Liang Xiang, Xin-Jie Jin, Chao Shen, Xia-Fang Cheng, Lei Shu, Rui-Liang Zhu","doi":"10.1111/cla.12513","DOIUrl":"10.1111/cla.12513","url":null,"abstract":"<p>Marchantiopsida (complex thalloid liverworts) are one of the earliest lineages of embryophytes (land plants), and well-known for their air pores and chambers, pegged rhizoids, and absence of organellular RNA editing sites. Despite their importance to an understanding of early embryophyte evolution, many key nodes within this class remain poorly resolved, owing to the paucity of genetic loci previously available for phylogenetic analyses. Here, we sequenced 54 plastomes, representing 28 genera, nearly all families, and all orders of Marchantiopsida. Based on these plastomes, we present a hypothesis of deep relationships within the class, and make the first investigations of gene contents and synteny. Overall, the Marchantiopsida plastomes were well-conserved, with the exception of the genus <i>Cyathodium</i> that has plastomes with higher GC content, fewer single sequence repeats (SSRs), and more structural variations, implying that this genus might possess RNA editing sites. Abundant repetitive elements and six highly divergent regions were identified as suitable for future infrafamilial taxonomic studies. The phylogenetic topology of Sphaerocarpales, Neohodgsoniales and Blasiales within Marchantiopsida was essentially congruent with previous studies but generally we obtained higher support values. Based on molecular evidence and previous morphological studies, we include Lunulariales in Marchantiales and suggest the retention of narrowed delimitation of monotypic families. The phylogenetic relationships within Marchantiales were better resolved, and 13 monophyletic families were recovered. Our analyses confirmed that the loss of intron 2 of <i>ycf3</i> is a synapomorphy of Marchantiidae. Finally, we propose a new genus, <i>Asterellopsis</i> (Aytoniaceae), and present an updated classification of Marchantiopsida. The highly supported phylogenetic backbone provided here establishes a framework for future comparative and evolutionary studies of the complex thalloid liverworts.</p>","PeriodicalId":50688,"journal":{"name":"Cladistics","volume":"38 6","pages":"649-662"},"PeriodicalIF":3.6,"publicationDate":"2022-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10631369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hyb-Seq provides new insights into the phylogeny and evolution of the Chrysanthemum zawadskii species complex in China","authors":"Wen-Xun Lu, Xue-Ying Hu, Zi-Zhao Wang, Guang-Yuan Rao","doi":"10.1111/cla.12514","DOIUrl":"10.1111/cla.12514","url":null,"abstract":"<p>A species complex is an assemblage of closely related species with blurred boundaries, and from which species could arise from different speciation processes and/or a speciation continuum. Such a complex can provide an opportunity to investigate evolutionary mechanisms acting on speciation. The <i>Chrysanthemum zawadskii</i> species complex in China, a monophyletic group of <i>Chrysanthemum</i>, consists of seven species with considerable morphological variation, diverse habitats and different distribution patterns. Here, we used Hyb-Seq data to construct a well-resolved phylogeny of the <i>C. zawadskii</i> complex. Then, we performed comparative analyses of variation patterns in morphology, ecology and distribution to investigate the roles of geography and ecology in this complex’s diversification. Lastly, we implemented divergence time estimation, species distribution modelling and ancestral area reconstruction to trace the evolutionary history of this complex. We concluded that the <i>C. zawadskii</i> complex originated in the Qinling–Daba mountains during the early Pliocene and then spread west and northward along the mountain ranges to northern China. During this process, geographical and ecological factors imposing different influences resulted in the current diversification and distribution patterns of this species complex, which is composed of both well-diverged species and diverging lineages on the path of speciation.</p>","PeriodicalId":50688,"journal":{"name":"Cladistics","volume":"38 6","pages":"663-683"},"PeriodicalIF":3.6,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9198626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CladisticsPub Date : 2022-06-27DOI: 10.1111/cla.12511
Weiting Zhang, Chungkun Shih, Michael S. Engel, Dong Ren
{"title":"Cretaceous lophocoronids with short proboscis and retractable female genitalia provide the earliest evidence for their feeding and oviposition habits","authors":"Weiting Zhang, Chungkun Shih, Michael S. Engel, Dong Ren","doi":"10.1111/cla.12511","DOIUrl":"10.1111/cla.12511","url":null,"abstract":"<p>We describe two new species of Lophocoronidae: <i>Acanthocorona hedida</i> Zhang, Shih and Engel <b>sp. n.</b> and <i>Acanthocorona venulosa</i> Zhang, Shih and Engel <b>sp. n.</b>, and an undetermined specimen from mid-Cretaceous Kachin amber. Phylogenetic analysis of basal lepidopteran lineages, including three extinct families, was undertaken. The analysis supported monophyly of Glossata although internal relationships remain controversial. <i>Acanthocorona</i> and <i>Lophocorona</i> form a monophyletic group. It is likely that short and simply structured proboscides of <i>Acanthocorona</i> were used to sip water droplets, pollination drops from gymnosperms, nectar from early flowers, or sap from injured leaves. Both retracted and extended ovipositors are preserved in the material reported here, revealing their morphology and indicating that these Cretaceous lophocoronids inserted eggs into the tissues of their host plants.</p>","PeriodicalId":50688,"journal":{"name":"Cladistics","volume":"38 6","pages":"684-701"},"PeriodicalIF":3.6,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10632633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CladisticsPub Date : 2022-05-15DOI: 10.1111/cla.12507
Mark P. Simmons, Olivier Maurin, Paul Bailey, Grace E. Brewer, Shyamali Roy, Julio A. Lombardi, Félix Forest, William J. Baker
{"title":"Benefits of alignment quality-control processing steps and an Angiosperms353 phylogenomics pipeline applied to the Celastrales","authors":"Mark P. Simmons, Olivier Maurin, Paul Bailey, Grace E. Brewer, Shyamali Roy, Julio A. Lombardi, Félix Forest, William J. Baker","doi":"10.1111/cla.12507","DOIUrl":"10.1111/cla.12507","url":null,"abstract":"<p>We examined the impact of successive alignment quality-control steps on downstream phylogenomic analyses. We applied a recently published phylogenomics pipeline that was developed for the Angiosperms353 target-sequence-capture probe set to the flowering plant order Celastrales. Our final dataset consists of 158 species, including at least one exemplar from all 109 currently recognized Celastrales genera. We performed nine quality-control steps and compared the inferred resolution, branch support, and topological congruence of the inferred gene and species trees with those generated after each of the first six steps. We describe and justify each of our quality-control steps, including manual masking, in detail so that they may be readily applied to other lineages. We found that highly supported clades could generally be relied upon even if stringent orthology and alignment quality-control measures had not been applied. But separate instances were identified, for both concatenation and coalescence, wherein a clade was highly supported before manual masking but then subsequently contradicted. These results are generally reassuring for broad-scale analyses that use phylogenomics pipelines, but also indicate that we cannot rely exclusively on these analyses to conclude how challenging phylogenetic problems are best resolved.</p>","PeriodicalId":50688,"journal":{"name":"Cladistics","volume":"38 5","pages":"595-611"},"PeriodicalIF":3.6,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10631355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CladisticsPub Date : 2022-04-30DOI: 10.1111/cla.12506
Armando J. Cruz-Laufer, Antoine Pariselle, Michiel W. P. Jorissen, Fidel Muterezi Bukinga, Anwar Al Assadi, Maarten Van Steenberge, Stephan Koblmüller, Christian Sturmbauer, Karen Smeets, Tine Huyse, Tom Artois, Maarten P. M. Vanhove
{"title":"Somewhere I belong: phylogeny and morphological evolution in a species-rich lineage of ectoparasitic flatworms infecting cichlid fishes","authors":"Armando J. Cruz-Laufer, Antoine Pariselle, Michiel W. P. Jorissen, Fidel Muterezi Bukinga, Anwar Al Assadi, Maarten Van Steenberge, Stephan Koblmüller, Christian Sturmbauer, Karen Smeets, Tine Huyse, Tom Artois, Maarten P. M. Vanhove","doi":"10.1111/cla.12506","DOIUrl":"https://doi.org/10.1111/cla.12506","url":null,"abstract":"<p>A substantial portion of biodiversity has evolved through adaptive radiation. However, the effects of explosive speciation on species interactions remain poorly understood. Metazoan parasites infecting radiating host lineages could improve our knowledge because of their intimate host relationships. Yet limited molecular, phenotypic and ecological data discourage multivariate analyses of evolutionary patterns and encourage the use of discrete characters. Here, we assemble new molecular, morphological and host range data widely inferred from a species-rich lineage of parasites (<i>Cichlidogyrus</i>, Platyhelminthes: Monogenea) infecting cichlid fishes to address data scarcity. We infer a multimarker (28S/18S rDNA, ITS1, COI mtDNA) phylogeny of 58 of 137 species and characterize major lineages through synapomorphies inferred from mapping morphological characters. We predict the phylogenetic position of species without DNA data through shared character states, a morphological phylogenetic analysis, and a classification analysis with support vector machines. Based on these predictions and a cluster analysis, we assess the systematic informativeness of continuous characters, search for continuous equivalents for discrete characters, and suggest new characters for morphological traits not analysed to date. We also model the attachment/reproductive organ and host range evolution using the data for 136 of 137 described species and multivariate phylogenetic comparative methods (PCMs). We show that discrete characters not only can mask phylogenetic signals, but also are key for characterizing species groups. Regarding the attachment organ morphology, a divergent evolutionary regime for at least one lineage was detected and a limited morphological variation indicates host and environmental parameters affecting its evolution. However, moderate success in predicting phylogenetic positions, and a low systematic informativeness and high multicollinearity of morphological characters call for a revaluation of characters included in species characterizations.</p>","PeriodicalId":50688,"journal":{"name":"Cladistics","volume":"38 4","pages":"465-512"},"PeriodicalIF":3.6,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91886340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CladisticsPub Date : 2022-04-27DOI: 10.1111/cla.12505
Ivan L. F. Magalhaes, Martín J. Ramírez
{"title":"Phylogeny and biogeography of the ancient spider family Filistatidae (Araneae) is consistent both with long-distance dispersal and vicariance following continental drift","authors":"Ivan L. F. Magalhaes, Martín J. Ramírez","doi":"10.1111/cla.12505","DOIUrl":"10.1111/cla.12505","url":null,"abstract":"<p>Filistatids, the crevice weavers, are an ancient family of cribellate spiders without extant close relatives. As one of the first lineages of araneomorph spiders, they present a complicated mixture of primitive and derived characters that make them a key taxon to elucidate the phylogeny of spiders, as well as the evolution of phenotypic characters in this group. Their moderate diversity (187 species in 19 genera) is distributed mainly in arid and semi-arid subtropical zones of all continents, except Antarctica. The objective of this paper is to generate a comprehensive phylogenetic hypothesis for this family to advance the understanding of its morphological evolution and biogeography, as well as lay the basis for a natural classification scheme. By studying the morphology using optical and electronic microscopy techniques, we produced a matrix of 302 morphological characters coded for a sample of 103 species of filistatids chosen to represent the phylogenetic diversity of the family. In addition, we included sequences of four molecular markers (COI, 16S, H3 and 28S; 3787 aligned positions) of 70 filistatid species. The analysis of the data (morphological, molecular, and combined) consistently indicates the separation of the Filistatidae into two subfamilies, Prithinae and Filistatinae, in addition to supporting several groups of genera: <i>Filistata, Zaitunia</i> and an undescribed genus from Madagascar; <i>Sahastata</i> and <i>Kukulcania</i>; all Prithinae except <i>Filistatinella</i> and <i>Microfilistata</i>; <i>Antilloides</i> and <i>Filistatoides</i>; a large Old World group including <i>Pritha</i>, <i>Tricalamus</i>, <i>Afrofilistata</i>, <i>Labahitha</i>, <i>Yardiella</i>, <i>Wandella</i> and putative new genera; and a South American group formed by <i>Lihuelistata</i>, <i>Pikelinia</i> and <i>Misionella</i>. <i>Pholcoides</i> is transferred to Filistatinae and <i>Microfilistata</i> is transferred to Prithinae, and each represents the sister group to the remaining genera of its own subfamily. Most genera are valid, although <i>Pikelinia</i> is paraphyletic with respect to <i>Misionella</i>, so we consider the two genera as synonyms and propose a few new generic combinations. Considering the new phylogenetic hypothesis, we discuss the evolution of some morphological character systems and the biogeography of the family. The ages of divergence between clades were estimated using a total-evidence tip-dating approach by including fossils of Filistatidae and early spider clades; this approach resulted in younger age estimates than those obtained with traditional node-dating. Filistatidae is an ancient family that started diversifying in the Mesozoic and most genera date to the Cretaceous. Clades displaying transcontinental distributions were most likely affected by continental drift, but at least one clade shows unequivocal signs of transoceanic long-distance dispersal.</p>","PeriodicalId":50688,"journal":{"name":"Cladistics","volume":"38 5","pages":"538-562"},"PeriodicalIF":3.6,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10637054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CladisticsPub Date : 2022-03-31DOI: 10.1111/cla.12502
Ya-Ping Chen, Turginov Orzimat Turdimatovich, Maxim S. Nuraliev, Predrag Lazarević, Bryan T. Drew, Chun-Lei Xiang
{"title":"Phylogeny and biogeography of the northern temperate genus Dracocephalum s.l. (Lamiaceae)","authors":"Ya-Ping Chen, Turginov Orzimat Turdimatovich, Maxim S. Nuraliev, Predrag Lazarević, Bryan T. Drew, Chun-Lei Xiang","doi":"10.1111/cla.12502","DOIUrl":"10.1111/cla.12502","url":null,"abstract":"<p>The northern temperate genus <i>Dracocephalum</i> consists of approximately 70 species mainly distributed in the steppe-desert biomes of Central and West Asia and the alpine region of the Qinghai-Tibetan Plateau (QTP). Previous work has shown that <i>Dracocephalum</i> is not monophyletic and might include <i>Hyssopus</i> and <i>Lallemantia</i>. This study attempts to clarify the phylogenetic relationships, diversification patterns, and the biogeographical history of the three genera (defined as <i>Dracocephalum</i> s.l.). Based on a sampling of 66 taxa comprising more than 80% from extant species of <i>Dracocephalum</i> s.l., morphological, phylogenetic (maximum parsimony, likelihood, and Bayesian inference based on nuclear ITS and ETS, plastid <i>rpl32-trnL</i>, <i>trnL-trnF</i>, <i>ycf1</i>, and <i>ycf1-rps15</i>, and two low-copy nuclear markers <i>AT3G09060</i> and <i>AT1G09680</i>), molecular dating, diversification, and ancestral range estimation analyses were carried out. Our results demonstrate that both <i>Hyssopus</i> and <i>Lallemantia</i> are embedded within <i>Dracocephalum</i> and nine well-supported clades can be recognized within <i>Dracocephalum</i> s.l. Analyses of divergence times suggest that the genus experienced an early rapid radiation during the middle to late Miocene with major lineages diversifying within a relatively narrow timescale. Ancestral area reconstruction analyses indicate that <i>Dracocephalum</i> s.l. originated in Central and West Asia and southern Siberia, and dispersed from Central and West Asia into the QTP and adjacent areas twice independently during the Pliocene. The aridification of the Asian interior possibly promoted the rapid radiation of <i>Dracocephalum</i> within this region, and the uplift of the QTP appears to have triggered the dispersal and recent rapid diversification of the genus in the QTP and adjacent regions. Combining molecular phylogenetic and morphological evidence, a revised infrageneric classification of <i>Dracocephalum</i> s.l. is proposed, which recognizes nine sections within the genus.</p>","PeriodicalId":50688,"journal":{"name":"Cladistics","volume":"38 4","pages":"429-451"},"PeriodicalIF":3.6,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47808397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
CladisticsPub Date : 2022-03-29DOI: 10.1111/cla.12501
Fei Ye, Petr Kment, Dávid Rédei, Jiu-Yang Luo, Yan-Hui Wang, Stefan M. Kuechler, Wei-Wei Zhang, Ping-Ping Chen, Hao-Yang Wu, Yan-Zhuo Wu, Xiao-Ya Sun, Lu Ding, Yue-Ran Wang, Qiang Xie
{"title":"Diversification of the phytophagous lineages of true bugs (Insecta: Hemiptera: Heteroptera) shortly after that of the flowering plants","authors":"Fei Ye, Petr Kment, Dávid Rédei, Jiu-Yang Luo, Yan-Hui Wang, Stefan M. Kuechler, Wei-Wei Zhang, Ping-Ping Chen, Hao-Yang Wu, Yan-Zhuo Wu, Xiao-Ya Sun, Lu Ding, Yue-Ran Wang, Qiang Xie","doi":"10.1111/cla.12501","DOIUrl":"10.1111/cla.12501","url":null,"abstract":"<p>More than 95% of phytophagous true bug (Hemiptera: Heteroptera) species belong to four superfamilies: Miroidea (Cimicomorpha), Pentatomoidea, Coreoidea, and Lygaeoidea (all Pentatomomorpha). These iconic groups of highly diverse, overwhelmingly phytophagous insects include several economically prominent agricultural and silvicultural pest species, though their evolutionary history has not yet been well resolved. In particular, superfamily- and family-level phylogenetic relationships of these four lineages have remained controversial, and the divergence times of some crucial nodes for phytophagous true bugs have hitherto been little known, which hampers a better understanding of the evolutionary processes and patterns of phytophagous insects. In the present study, we used 150 species and concatenated nuclear and mitochondrial protein-coding genes and rRNA genes to infer the phylogenetic relationships within the Terheteroptera (Cimicomorpha + Pentatomomorpha) and estimated their divergence times. Our results support the monophyly of Cimicomorpha, Pentatomomorpha, Miroidea, Pentatomoidea, Pyrrhocoroidea, Coreoidea, and Lygaeoidea. The phylogenetic relationships across phytophagous lineages are largely congruent at deep nodes across the analyses based on different datasets and tree-reconstructing methods with just a few exceptions. Estimated divergence times and ancestral state reconstructions for feeding habit indicate that phytophagous true bugs explosively radiated in the Early Cretaceous—shortly after the angiosperm radiation—with the subsequent diversification of the most speciose clades (Mirinae, Pentatomidae, Coreinae, and Rhyparochromidae) in the Late Cretaceous.</p>","PeriodicalId":50688,"journal":{"name":"Cladistics","volume":"38 4","pages":"403-428"},"PeriodicalIF":3.6,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44998615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Highly diversified mitochondrial genomes provide new evidence for interordinal relationships in the Arachnida","authors":"Xin-Chao Ban, Zi-Kai Shao, Li-Jun Wu, Jing-Tao Sun, Xiao-Feng Xue","doi":"10.1111/cla.12504","DOIUrl":"10.1111/cla.12504","url":null,"abstract":"<p>Arachnida is an exceptionally diverse class in the Arthropoda, consisting of 20 orders and playing crucial roles in the terrestrial ecosystems. However, their interordinal relationships have been debated for over a century. Rearranged or highly rearranged mitochondrial genomes (mitogenomes) were consistently found in this class, but their various extent in different lineages and efficiency for resolving arachnid phylogenies are unclear. Here, we reconstructed phylogenetic trees using mitogenome sequences of 290 arachnid species to decipher interordinal relationships as well as diversification through time. Our results recovered monophyly of ten orders (i.e. Amblypygi, Araneae, Ixodida, Mesostigmata, Opiliones, Pseudoscorpiones, Ricinulei, Sarcoptiformes, Scorpiones and Solifugae), while rejecting monophyly of the Trombidiformes due to the unstable position of the Eriophyoidea. The monophyly of Acari (subclass) was rejected, possibly due to the long-branch attraction of the Pseudoscorpiones. The monophyly of Arachnida was further rejected because the Xiphosura nested within arachnid orders with unstable positions. Mitogenomes that are highly rearranged in mites but less rearranged or conserved in the remaining lineages point to their exceptional diversification in mite orders; however, shared derived mitochondrial (mt) gene clusters were found within superfamilies rather than interorders, confusing phylogenetic signals in arachnid interordinal relationships. Molecular dating results show that arachnid orders have ancient origins, ranging from the Ordovician to the Carboniferous, yet have significantly diversified since the Cretaceous in orders Araneae, Mesostigmata, Sarcoptiformes, and Trombidiformes. By summarizing previously resolved key positions of some orders, we propose a plausible arachnid tree of life. Our results underline a more precise framework for interordinal phylogeny in the Arachnida and provide new insights into their ancient evolution.</p>","PeriodicalId":50688,"journal":{"name":"Cladistics","volume":"38 4","pages":"452-464"},"PeriodicalIF":3.6,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41539592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}