Clinical & Translational Oncology最新文献

{"title":"SEOM-GEMCAD-TTD clinical guidelines for biliary tract cancer (2025).","authors":"Teresa Macarulla, Jorge Adeva, Maria Teresa Cano Osuna, Ana Ruiz Casado, Ana María Jiménez Gordo, Angela Lamarca, Ana María López Muñoz, Roberto Antonio Pazo Cid, Tamara Saurí, Javier Gallego Plazas","doi":"10.1007/s12094-025-03916-y","DOIUrl":"https://doi.org/10.1007/s12094-025-03916-y","url":null,"abstract":"<p><p>Biliary tract cancers (BTC) are aggressive and fatal. Early recognition of symptoms and proper diagnostic work up allow for precise histopathological and molecular classification as well as accurate evaluation of the extent of disease. Surgery is the only potentially curative therapy in localized stages; however, disease recurrence is common and adjuvant chemotherapy appears to improve survival. Upfront systemic chemotherapy with immunotherapy is the treatment of choice in unresectable locally-advanced and metastatic disease. Inroads made in understanding its molecular biology has enabled new therapeutic targets to be identified with current indications and encouraging results that could further improve BTC patients' survival and quality of life.</p>","PeriodicalId":50685,"journal":{"name":"Clinical & Translational Oncology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-arm study of sarcopenia in geriatric patients with high-grade glial tumors: temporal muscle thickness and prognostic nutritional index.","authors":"Hatice Basaran Goksen, Alaettin Arslan, Hasan Erdogan","doi":"10.1007/s12094-025-03951-9","DOIUrl":"https://doi.org/10.1007/s12094-025-03951-9","url":null,"abstract":"<p><strong>Purpose: </strong>To analyze the prognostic significance of temporal muscle thickness (TMT) and prognostic nutritional index (PNI) in the assessment of sarcopenia in patients diagnosed with glioblastoma multiforme (GBM) in the geriatric age group.</p><p><strong>Methods: </strong>This study included a total of 50 patients (≥65 years of age) receiving radiotherapy (RT)/radio-chemotherapy (RCT) at Kayseri City Education and Research Hospital. We calculated TMT from cranial magnetic resonance imaging (MRI) at the time of diagnosis and PNI from laboratory measurements. To determine the cut-off values, we used the ROC analysis. The combined group (CG) was formed according to TMT and PNI.</p><p><strong>Results: </strong>Median overall survival (OS) was 5 months. OS for those with TMT < 6.75 cm and ≥ 6.75 cm was 5 months and 11 months, respectively (p < 0.001). OS for those with PNI < 48.87 cm and ≥ 48.87 cm was 5 months and 11 months, respectively (p = 0.026). Survival analysis showed a dramatic difference in CG between groups 1 and 4 (16 months vs. 4 months, p < 0.001). Correlations were observed between TMT and age, excision type and OS (r = 0.406 p = 0.003, r = 0.346 p = 0.014, r = 0.345 p = 0.014). Univariate Cox regression analysis showed that age, excision type, diagnosis type, RT history, initial tumor volume (ITV), TMT, PNI, CG, and albumin had an effect on survival (p values 0.017, <0.001, <0.001, <0.001, 0.011, <0.001, 0.041, 0.001, and 0.018, respectively). Multivariate Cox regression analysis showed that excision type, RT history, ITV, albumin, and CG were independent factors affecting survival (p values 0.007, 0.05, 0.005, 0.029, and 0.014, respectively).</p><p><strong>Conclusion: </strong>Although both parameters individually have a prognostic effect in geriatric GBM patients, their combined effect as a single parameter has a much stronger impact on prognosis.</p>","PeriodicalId":50685,"journal":{"name":"Clinical & Translational Oncology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutational landscape and clinical implications of VHL in clear cell renal cell carcinoma: a multi-dataset analysis of 1377.","authors":"Qiang Tian, Wenwen Tang, Xiaoming Cao","doi":"10.1007/s12094-025-03954-6","DOIUrl":"https://doi.org/10.1007/s12094-025-03954-6","url":null,"abstract":"<p><strong>Purpose: </strong>Kidney cancer stands as a threat worldwide, with clear cell renal cell carcinoma (ccRCC) emerging as its predominant subtype. Through the establishment of extensive databases, the somatic mutations associated with ccRCC are successfully pinpointed. The tumor suppressor gene Von Hippel-Lindau (VHL) is commonly mutated in ccRCC.</p><p><strong>Objective: </strong>In this study, we aim to analyze different cBIOPortal datasets to explore VHL mutation frequencies in ccRCC.</p><p><strong>Methods: </strong>The datasets explored were Kidney Renal Clear Cell Carcinoma (TCGA, Nature 2013), Kidney Renal Clear Cell Carcinoma (IRC, Nat Genet 2014), Kidney Renal Clear Cell Carcinoma (TCGA, Firehose Legacy), Kidney Renal Clear Cell Carcinoma (TCGA, PanCancer Atlas). Data mining from various datasets revealed that VHL is the most mutated gene, with mutation frequencies of 79.5%, 51.2%, 49.9%, and 41.3% across different datasets: Kidney Renal Clear Cell Carcinoma (IRC, Nat Genet 2014), Kidney Renal Clear Cell Carcinoma (TCGA, Nature 2013), Kidney Renal Clear Cell Carcinoma (TCGA, Firehose Legacy), and Kidney Renal Clear Cell Carcinoma (TCGA, PanCancer Atlas), respectively.</p><p><strong>Results: </strong>The mutated VHL gene is associated with significantly reduced overall survival (OS) rates based on the analyses of these datasets. VHL mutation becomes more advanced at a late age with many distant metastases.</p><p><strong>Conclusion: </strong>This data confirms the mutational burden of VHL in ccRCC and suggests it is a potential therapeutic target for the management of ccRCC.</p>","PeriodicalId":50685,"journal":{"name":"Clinical & Translational Oncology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144163485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of microsatellite status on tertiary lymphoid structures and lymph node metastasis in colorectal cancer.","authors":"Kai Wang, Shizhen Zhou, Hao Wang, Xufei Zhang, Wei Cheng, Yan Sun, Chao Ding, Wenxian Guan","doi":"10.1007/s12094-025-03946-6","DOIUrl":"https://doi.org/10.1007/s12094-025-03946-6","url":null,"abstract":"<p><strong>Background: </strong>Microsatellite stability influences the prognosis of patients with colorectal cancer (CRC). However, there are few studies on the relationship between microsatellite stability and lymph node metastasis (LNM) in CRC.</p><p><strong>Objective: </strong>This study aims to elucidate the relationship between microsatellite stability and LNM in CRC and to investigate potential underlying mechanisms.</p><p><strong>Methods: </strong>A retrospective analysis was performed on a cohort of 309 CRC patients, who were categorized into microsatellite instability (MSI) and microsatellite stability (MSS) groups based on their microsatellite status. Clinical and pathological indicators were collected, and differences between the two groups were assessed. The tertiary lymphoid structures (TLS) in both groups were examined using immunohistochemistry and immunofluorescence to compare differences in density, maturity, and the ratio of CD8+T cells. Establishing the relationship between microsatellite stability, TLS characteristics, and lymph node metastasis.</p><p><strong>Results: </strong>The TNM staging for patients in the MSI group was significantly earlier compared to those in the MSS group. Subsequent analysis of pathological indicators demonstrated that the MSI group exhibited a significantly lower incidence of lymph node metastases (31.4% vs. 47%, P=0.005), while no statistically significant differences were observed in other pathological indicators (P>0.05). Examination of CRC tissue sections revealed that the MSI group possessed a greater number and maturity of tertiary lymphoid structures, as well as a higher proportion of CD8+T cells.</p><p><strong>Conclusion: </strong>MSI may decrease the incidence of LNM in CRC, potentially as a result of the activation of local anti-tumor immune responses facilitated by MSI.</p>","PeriodicalId":50685,"journal":{"name":"Clinical & Translational Oncology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heart substructure exposure during left breast cancer radiotherapy: a dosimetric comparison between hybrid VMAT and 3DCRT in free breathing and deep inspiration breath hold (DIBH).","authors":"Ingrid Romera-Martínez, Irene Oliveras Cancio, Maria Buxó, Joan Martínez, Rafael Fuentes-Raspall, Arantxa Eraso Urién, Joan Carles Vilanova","doi":"10.1007/s12094-025-03943-9","DOIUrl":"https://doi.org/10.1007/s12094-025-03943-9","url":null,"abstract":"<p><strong>Background and purpose: </strong>Radiotherapy plays a pivotal role in breast cancer treatment. Incidental irradiation of the heart can cause cardiac toxicity. We conducted a dosimetric comparison between Hybrid VMAT (H-VMAT) and 3D Conformal Radiotherapy (3DCRT) in both Free Breathing (FB) and Deep inspiration breath hold (DIBH) techniques for left-sided breast cancer patients.</p><p><strong>Materials and methods: </strong>Thirty-seven patients underwent CT scans in both FB and DIBH positions. Heart substructures were delineated following established guidelines. Subsequently, 3DCRT and H-VMAT plans were generated for each patient in both breath techniques. The dosimetric parameters of heart and its cavities were analysed: D_mean(Gy) and V5Gy(%) for heart substructures, D_mean(Gy), V25Gy(%) and V30Gy(cm³) for heart and D98%(%), D2%(%), D50%(%) and V95%(%) or V90%(%) for PTVs. Statistical analyses were performed.</p><p><strong>Results: </strong>The analysis revealed statistically significant differences for the heart, and its cavities. The 3DCRT plans generated in DIBH offered a statistically significant lower dose for the heart and its substructures compared to the other techniques. When comparing PTV dosimetry, the H-VMAT plans showed an increase D98%(%) and a decrease D2%(%) relative to the 3DCRT plans, for both breathing techniques employed.</p><p><strong>Conclusion: </strong>Our study demonstrates significant differences in the dosimetric outcomes for the heart substructures among the four evaluated techniques, with 3DCRT in DIBH yielding the lowest parameters for most substructures. Although H-VMAT provided superior target coverage, it resulted in greater low doses incidental exposure of the heart substructures. Notably, 3DCRT plans in the DIBH setting exhibited lower doses compared to H-VMAT in FB, supporting its preferential use for minimising cardiac exposure.</p>","PeriodicalId":50685,"journal":{"name":"Clinical & Translational Oncology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stable mood and high enjoyment in breast cancer patients: positive experience with exercise during chemotherapy infusion.","authors":"Diego Bessa Dantas, Vinicius Muller Reis Weber, Regiane Bueno Araújo, Rosa de Lourdes Beltrão, Timothy Gustavo Cavazzotto, Danilo Fernandes da Silva, Marcos Roberto Queiroga","doi":"10.1007/s12094-025-03952-8","DOIUrl":"https://doi.org/10.1007/s12094-025-03952-8","url":null,"abstract":"<p><strong>Objective: </strong>Considering the long waiting time during chemotherapy as an opportunity to stay active, this experiment proposes to investigate the experience of breast cancer patients performing exercise during chemotherapy infusion, focusing on mood stability and levels of enjoyment.</p><p><strong>Methods: </strong>Adult female oncology patients (24) undergoing chemotherapy were recruited to perform 20 minutes of low-intensity pedaling on a cycle ergometer (30-40% HRR) starting 10 minutes after the beginning of the chemotherapy infusion. Physical activity levels were assessed prior to the exercise session, while mood states were measured before and after the activity. Additionally, the feeling scale and the enjoyment levels were collected after the activity.</p><p><strong>Results: </strong>The results indicated that the exercise did not significantly alter patients' mood, maintaining stability between pre- and post-activity assessments (p>0.05). Additionally, high affectivity levels were observed on the feeling scale (4.33 ± 0.86), along with the elevated enjoyment levels (106.9 ± 16.1), regardless of patients' prior physical activity levels. These findings suggest that the exercise was well-received and provided a positive experience during chemotherapy infusion.</p><p><strong>Conclusion: </strong>The findings of this study suggest that low-intensity exercise during chemotherapy infusion in breast cancer patients is feasible and well-tolerated, providing a positive experience without adverse effects on mood. These results underscore the potential of light exercise as a complementary intervention to promote emotional well-being and enhance treatment adherence, offering new perspectives for improving quality of life during chemotherapy.</p>","PeriodicalId":50685,"journal":{"name":"Clinical & Translational Oncology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a prognostic nomogram for diffuse large B-cell lymphoma: a multicenter cohort study from Northern China.","authors":"Juya Cui, Tao Wang, Junyan Zhang, Meiru Guo, Jun Zhu, Shuling Hou","doi":"10.1007/s12094-025-03955-5","DOIUrl":"https://doi.org/10.1007/s12094-025-03955-5","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to refine the prognostic stratification provided by the International Prognostic Index (IPI) for patients diagnosed with diffuse large B-cell lymphoma (DLBCL) in a northern Chinese cohort and to construct a more precise prognostic nomogram to facilitate individualized treatment strategies and improve survival outcomes.</p><p><strong>Methods: </strong>Clinical data from 1,364 patients diagnosed with DLBCL between January 2000 and December 2019 were retrospectively analyzed across multiple centers in Beijing and Shanxi, China. A prognostic nomogram model for overall survival (OS) was developed incorporating the following variables: age, disease stage, Eastern Cooperative Oncology Group (ECOG) performance status, lactate dehydrogenase (LDH) levels, involvement of various extranodal sites, and disease progression within 6 months (POD6). Model performance was evaluated through receiver-operating characteristic (ROC) curve analysis, calibration curves, concordance index (C-index), and area under the curve (AUC) metrics.</p><p><strong>Results: </strong>The developed nomogram demonstrated consistent predictive capacity, with AUC values exceeding 0.7 at 1-, 3-, and 5-year time points. Calibration plots indicated close alignment with the ideal reference line, with slopes approximating 1, supporting the model's predictive accuracy and clinical relevance. POD6 was identified as the most significant high-risk factor associated with decreased OS (hazard ratio [HR] = 5.13, 95% confidence interval [CI]: 1.03-2.51, p < 0.0001). Among the IPI components, all except ECOG performance status and extranodal involvement remained significant. Notably, central nervous system involvement exhibited the strongest adverse prognostic effect among extranodal sites (HR = 1.61, 95% CI: 1.03-2.51, p = 0.035).</p><p><strong>Conclusion: </strong>A novel prognostic nomogram was established for patients with DLBCL, offering improved prognostic accuracy compared to the traditional IPI. This model presents clinical utility in supporting personalized management and treatment planning.</p><p><strong>Clinical registration number: </strong>Not applicable.</p>","PeriodicalId":50685,"journal":{"name":"Clinical & Translational Oncology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence in neuro-oncology: methodological bases, practical applications and ethical and regulatory issues.","authors":"Pedro David Delgado-López, Miguel Cárdenas Montes, Jesús Troya García, Beatriz Ocaña-Tienda, Santiago Cepeda, Ricard Martínez Martínez, Eva María Corrales-García","doi":"10.1007/s12094-025-03948-4","DOIUrl":"https://doi.org/10.1007/s12094-025-03948-4","url":null,"abstract":"<p><p>Artificial Intelligence (AI) is transforming neuro-oncology by enhancing diagnosis, treatment planning, and prognosis prediction. AI-driven approaches-such as CNNs and deep learning-have improved the detection and classification of brain tumors through advanced imaging techniques and genomic analysis. Explainable AI methods mitigate the \"black box\" problem, promoting model transparency and clinical trust. Mechanistic models complement AI by integrating biological principles, enabling precise tumor growth predictions and treatment response assessments. AI applications also include the creation of digital twins for personalized therapy optimization, virtual clinical trials, and predictive modeling for estimation of tumor resection and pattern of recurrence. However, challenges such as data bias, ethical concerns, and regulatory compliance persist. The European Artificial Intelligence Act and the Health Data Space Regulation impose strict data protection and transparency requirements. This review explores AI's methodological foundations, clinical applications, and ethical challenges in neuro-oncology, emphasizing the need for interdisciplinary collaboration and regulatory adaptation.</p>","PeriodicalId":50685,"journal":{"name":"Clinical & Translational Oncology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of citrus essential oil petit grain in growth and motility reduction of hypopharyngeal squamous cell carcinoma (FaDu) in vitro model.","authors":"Veronica Russo, Maria Antonietta Oliva, Rossella Ricchiuti, Marika Alborghetti, Antonietta Arcella","doi":"10.1007/s12094-025-03931-z","DOIUrl":"https://doi.org/10.1007/s12094-025-03931-z","url":null,"abstract":"<p><strong>Background: </strong>Petit grain (PGO), a citrus essential oil (EO), isolated from the twigs and leaves of bitter orange (citrus arantiun), has been shown to have different beneficial properties, such as antibacterial, anti-fungal, anti-viral, anti-inflammatory and anti-cancer properties. Hypopharyngeal carcinoma is typically treated with combination radiotherapy and chemotherapy (cisplatin). However, resistance to cisplatin may lead to other new treatments, such as immunotherapy, which can be well-tolerated and potentially beneficial.</p><p><strong>Purpose: </strong>In this study, PGO was proposed as a natural growth-regulating molecule of the FaDu cell line from hypopharyngeal squamous cell carcinoma.</p><p><strong>Methods: </strong>The study used MTT assay, growth curves, Western blot, and FACS analysis to assess the impact of PGO on petit grain cell growth, protein levels, and cell cycle phases.</p><p><strong>Results: </strong>The potent cytotoxic effect of PGO against FaDu was demonstrated by its low IC50. Daily administration of 0.025% (v/v) and 0.5% (v/v) PGO on FaDu cells resulted in a time and dose-dependent decrease in cell viability and growth. PGO caused a cell cycle arrest in the G1/S transition phase, which was verified by increased expression of cell cycle regulating proteins p27, p21 and p18, and a decrease of CDK2 and CDK4 proteins. PGO treatment also reduced the phosphorylation of the proliferative signals pAKT. Last but not least, PGO dramatically increased the sensitivity of FaDu cells to chemotherapy when combined with cis-platin, the usual treatment for squamous cell carcinoma.</p><p><strong>Conclusion: </strong>Since PGO inhibits the growth of FaDu squamous carcinoma cells through a variety of combined mechanisms, it could be considered as a promising adjuvant therapy for this complex disease.</p>","PeriodicalId":50685,"journal":{"name":"Clinical & Translational Oncology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological insights and prognostic implications of DEK in association with apoptosis-regulating factors in ovarian cancer.","authors":"Trisha Choudhury, Ranita Pal, Madhurima Ghosh, Sriparna Chatterjee, Sinjini Sarkar, Manisha Vernekar, Partha Nath, Vilas D Nasare","doi":"10.1007/s12094-025-03944-8","DOIUrl":"https://doi.org/10.1007/s12094-025-03944-8","url":null,"abstract":"<p><strong>Purpose: </strong>Ovarian cancer is one of the most common gynecologic malignancies of the present era. Dysregulation of apoptosis is considered as one of the most important factors for malignant transformation. DEK is a ubiquitous protein, and its downregulation induces apoptosis by altering BCL-2, BAX, and CASPASE-3 expressions. This study illuminates the cumulative clinical usefulness of DEK and related apoptotic proteins.</p><p><strong>Methods: </strong>A total of 119 patients were enrolled during 2021-2023. Demographic and clinicopathological data were recorded at presentation, and the follow-up was done till August 2024. Tissue samples were analyzed using immunohistochemistry and real-time PCR. A paired t test assessed gene expression between normal and malignant tissues of different treatment strategies. The crosstab was performed to find the association of DEK, BCL-2, BAX, and CASPASE-3 with clinicopathological features. Pearson's correlation was used to predict the association of DEK with other apoptotic factors. Survival and hazard risk were evaluated using log-rank and Cox regression.</p><p><strong>Results: </strong>The mean age of OC patients was 47.61 ± 12.5 years, presented with advanced stage (90.7%) and grade (85.3%). Most of them were post-menopausal (68.08%) and had unhygienic (76.5%) regular menstrual cycles (89.1%), and also experienced early pregnancy (61.8%). Some of these factors are related to a hazard risk (HR > 1). DEK and apoptotic proteins were upregulated in OC than in normal (p ≤ 0.01). DEK was positively correlated with BCL-2, BAX, and CASPASE-3, at both mRNA and protein levels, and only BAX showed significance in both (p ≤ 0.05). All the selected genes are independent risk factors for survival of OC (HR > 1), but only DEK and CASPASE-3 were significantly associated with poor survival (p ≤ 0.05).</p><p><strong>Conclusions: </strong>Dysregulation of DEK, CASPASE-3, and BAX/BCL-2 is associated with poor overall survival. Further, this study highlights the correlation between DEK and key apoptotic regulators, emphasizing the critical role of DEK in OC prognosis.</p>","PeriodicalId":50685,"journal":{"name":"Clinical & Translational Oncology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}