Current Opinion in Pharmacology最新文献_第5页

Interventional functional diagnostics in gastrointestinal endoscopy: Combining diagnostic and therapeutic tools in the endoscopy suite with the functional lumen imaging probe 胃肠内窥镜介入功能诊断:将内窥镜套件中的诊断和治疗工具与功能性管腔成像探头相结合

IF 4 3区医学

Current Opinion in Pharmacology Pub Date : 2023-11-28 DOI: 10.1016/j.coph.2023.102414

Zuzana Vackova , Ian Levenfus , Daniel Pohl

{"title":"Interventional functional diagnostics in gastrointestinal endoscopy: Combining diagnostic and therapeutic tools in the endoscopy suite with the functional lumen imaging probe","authors":"Zuzana Vackova , Ian Levenfus , Daniel Pohl","doi":"10.1016/j.coph.2023.102414","DOIUrl":"https://doi.org/10.1016/j.coph.2023.102414","url":null,"abstract":"<div><p>With technical progress of gastrointestinal functional testing, there has been a demand for more comprehensive examination of esophageal physiology and pathophysiology beyond high-resolution manometry. A new interventional technology based on impedance planimetry, the functional lumen imaging probe (FLIP), enables intraluminal measurement of distensibility and compliance of hollow organs. EndoFLIP uses balloon catheters to measure diameter and distension pressure to calculate cross-sectional area and distensibility in different organs (mostly esophagus, stomach, anorectal region) and can be used in wide variety of indications (diagnostics, pre- and post-treatment evaluation) and currently serves as a helpful adjunctive tool in ambiguous clinical cases. EsoFLIP is a therapeutic variation that uses a stiffer balloon catheter allowing for dilation. The trend to simplify the clinical process from diagnosis to treatment tends to a one-session procedure combining diagnostics and therapeutic interventions. In specified conditions like e.g. achalasia or gastroparesis, a combination of EndoFLIP and EsoFLIP procedures may therefore be useful. The aim of this narrative review is to introduce the clinical use of FLIP and its potential benefit in combined diagnostic-therapeutic procedures.</p></div>","PeriodicalId":50603,"journal":{"name":"Current Opinion in Pharmacology","volume":"73 ","pages":"Article 102414"},"PeriodicalIF":4.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1471489223000693/pdfft?md5=a85fbc9fb6d7221a1db26a1995fcc341&pid=1-s2.0-S1471489223000693-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138453586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Illuminating GPCR signaling mechanisms by NMR spectroscopy with stable-isotope labeled receptors 用稳定同位素标记的受体通过NMR光谱阐明GPCR信号机制。

IF 4 3区医学

Current Opinion in Pharmacology Pub Date : 2023-10-01 DOI: 10.1016/j.coph.2023.102364

Beining Jin, Naveen Thakur, Anuradha V. Wijesekara, Matthew T. Eddy

引用次数: 1

Pharmacologic treatment of gastroparesis: What is (still) on the horizon? 胃轻瘫的药物治疗：什么（仍然）在地平线上？

IF 4 3区医学

Current Opinion in Pharmacology Pub Date : 2023-10-01 DOI: 10.1016/j.coph.2023.102395

D. Schweckendiek, D. Pohl

引用次数: 0

Discerning conformational dynamics and binding kinetics of GPCRs by 19F NMR 用19F NMR鉴别GPCR的构象动力学和结合动力学

IF 4 3区医学

Current Opinion in Pharmacology Pub Date : 2023-10-01 DOI: 10.1016/j.coph.2023.102377

R.S. Prosser , Nicholas A. Alonzi

{"title":"Discerning conformational dynamics and binding kinetics of GPCRs by 19F NMR","authors":"R.S. Prosser , Nicholas A. Alonzi","doi":"10.1016/j.coph.2023.102377","DOIUrl":"https://doi.org/10.1016/j.coph.2023.102377","url":null,"abstract":"<div><p><sup>19</sup>F NMR provides a way of monitoring conformational dynamics of G-protein coupled receptors (GPCRs) from the perspective of an ensemble. While X-ray crystallography provides exquisitely resolved high-resolution structures of specific states, it generally does not recapitulate the true ensemble of functional states. Fluorine (<sup>19</sup>F) NMR provides a highly sensitive spectroscopic window into the conformational ensemble, generally permitting the direct quantification of resolvable states. Moreover, straightforward T<sub>1</sub>- and T<sub>2</sub><span>-based relaxation experiments allow for the study of fluctuations within a given state and exchange between states, on timescales spanning nanoseconds to seconds. Conveniently, most biological systems are free of fluorine. Thus, via fluorinated amino acid analogues or thiol-reactive fluorinated tags, F or CF</span><sub>3</sub> reporters can be site specifically incorporated into proteins of interest. In this review, fluorine labeling protocols and <sup>19</sup>F NMR experiments will be presented, from the perspective of small molecule NMR (i.e. drug or small molecule interactions with receptors) or macromolecular NMR (i.e. conformational dynamics of receptors and receptor–G-protein complexes).</p></div>","PeriodicalId":50603,"journal":{"name":"Current Opinion in Pharmacology","volume":"72 ","pages":"Article 102377"},"PeriodicalIF":4.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50172845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of Pannexin-1 channels, ATP, and purinergic receptors in the pathogenesis of HIV and SARS-CoV-2 Pannexin-1通道、ATP和嘌呤能受体在HIV和严重急性呼吸系统综合征冠状病毒2型发病机制中的作用。

IF 4 3区医学

Current Opinion in Pharmacology Pub Date : 2023-09-19 DOI: 10.1016/j.coph.2023.102404

Cristian A. Hernandez, Eliseo A. Eugenin

引用次数: 0

Spatiotemporal GPCR signaling illuminated by genetically encoded fluorescent biosensors 基因编码荧光生物传感器照射的时空GPCR信号

IF 4 3区医学

Current Opinion in Pharmacology Pub Date : 2023-08-01 DOI: 10.1016/j.coph.2023.102384

Charlotte Kayser , Barbora Melkes , Cécile Derieux , Andreas Bock

{"title":"Spatiotemporal GPCR signaling illuminated by genetically encoded fluorescent biosensors","authors":"Charlotte Kayser , Barbora Melkes , Cécile Derieux , Andreas Bock","doi":"10.1016/j.coph.2023.102384","DOIUrl":"10.1016/j.coph.2023.102384","url":null,"abstract":"<div><p><span>G protein-coupled receptors (GPCRs) are ligand-activated cell membrane proteins and represent the most important class of drug targets. GPCRs adopt several active conformations that stimulate different intracellular </span>G proteins<span> (and other transducers) and thereby modulate second messenger levels, eventually resulting in receptor-specific cell responses. It is increasingly accepted that not only the type of active signaling protein but also the duration of its stimulation and the subcellular location from where receptors signal distinctly contribute to the overall cell response. However, the molecular principles governing such spatiotemporal GPCR signaling and their role in disease are incompletely understood. Genetically encoded, fluorescent biosensors—in particular for the GPCR/cAMP signaling axis—have been pivotal to the discovery and molecular understanding of novel concepts in spatiotemporal GPCR signaling. These include GPCR priming, location bias, and receptor-associated independent cAMP nanodomains. Here, we review such technologies that we believe will illuminate the spatiotemporal organization of other GPCR signaling pathways that define the complex signaling architecture of the cell.</span></p></div>","PeriodicalId":50603,"journal":{"name":"Current Opinion in Pharmacology","volume":"71 ","pages":"Article 102384"},"PeriodicalIF":4.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9920447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Central and peripheral mechanisms involved in the control of GnRH neuronal function by metabolic factors 代谢因子控制GnRH神经元功能的中枢和外周机制

IF 4 3区医学

Current Opinion in Pharmacology Pub Date : 2023-08-01 DOI: 10.1016/j.coph.2023.102382

Miguel Ruiz-Cruz , Carmen Torres-Granados , Manuel Tena-Sempere , Juan Roa

引用次数: 0

Corrigendum to “Antihypertensive effect of soybean bioactive peptides: A review” [Curr Opin Pharmacol (62) (2022) 74–81] 更正“大豆生物活性肽的降压作用：综述”[Curr Opin Pharmacol（62）（2022）74-81]

IF 4 3区医学

Current Opinion in Pharmacology Pub Date : 2023-08-01 DOI: 10.1016/j.coph.2023.102383

Tingna Li , Xiaorui Zhang , Yuanyuan Ren , Yijia Zeng , Qinwan Huang , Chao Wang

引用次数: 0

Targeting cAMP signaling compartments in iPSC-derived models of cardiovascular disease iPSC衍生的心血管疾病模型中的靶向cAMP信号区

IF 4 3区医学

Current Opinion in Pharmacology Pub Date : 2023-08-01 DOI: 10.1016/j.coph.2023.102392

Tiannan Liu , Enno Klussmann

引用次数: 0

Spinal and bulbar muscular atrophy: From molecular pathogenesis to pharmacological intervention targeting skeletal muscle 脊髓和延髓肌萎缩：从分子发病机制到针对骨骼肌的药物干预

IF 4 3区医学

Current Opinion in Pharmacology Pub Date : 2023-08-01 DOI: 10.1016/j.coph.2023.102394

Caterina Marchioretti , Roberta Andreotti , Emanuela Zuccaro , Andrew P. Lieberman , Manuela Basso , Maria Pennuto

引用次数: 0