Bionatura最新文献

{"title":"Detection of bla-AIM Metallo Beta Lactamase Gene among Stenotrophomonas Maltophilia and Carbapenem Resistant Pseudomonas Aeruginosa Isolated from Various Infections in AL- Najaf Province","authors":"Enas Jalil Baqer AL-Mayali, S. Al-Muhanna, Israa Abdul Ameer Al-Kraety","doi":"10.21931/rb/2024.09.01.61","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.61","url":null,"abstract":"Stenotrophomonas maltophilia is a \"rapidly evolving pathogen of concern\" that is increasingly being identified. The World Health Organization also recognizes it as one of the hospitals' most significant multi-drugresistant pathogens. Also, Pseudomonas aeruginosa is an opportunistic human pathogen that causes most\u0000healthcare-associated infections, and it is considered a paradigm of antibiotic resistance development. In many\u0000hospitals across the globe, carbapenem-resistant Pseudomonas aeruginosa has emerged as a significant source\u0000of infection. The present study aimed to study the isolation and diagnosis of S. maltophilia and P. aeruginosa\u0000from different clinical samples, evaluate the occurrence of carbapenem resistance of P. aeruginosa isolated\u0000from clinical samples, and investigate the dissemination of the bla-AIM genes between these isolates. Eight\u0000hundred fifty specimens were collected from various clinical samples between 2022 and 2023. The specimens\u0000included 220 swabs (burn), 200 (urine), 140 (stool), and 130(wound). 90 (ear),50 (throat), 10 (Cerebrospinal\u0000fluid), and 10 (blood). Represented by 680 specimens contained bacterial growth, and 170 specimens had no\u0000bacterial growth. Out of the 680 bacterial growth isolates, 410 revealed growths of Gram-negative bacteria,\u0000and 270 were Gram-positive bacteria. On MacConkey ag, ar 180/410 bacteria were lactose ferment; other\u0000isolates, es 230/410 of the isolates were lactose non-fermented bacteria. In a cross-sectional manner, Stenotrophomonas maltophilia and Pseudomonas aeruginosa isolates during this period were isolated and identified\u0000depending on the primary methods of diagnosis, then the use of the VITEK-2 compact system. The results\u0000showed 42 isolates of S. maltophilia and 80 isolates of P. aeruginosa from total Gram-negative bacteria. The\u0000results show that only five isolates contained the AIM gene, with a percentage of (10.4 %) of the 48 Carbapenem Resistant Pseudomonas aeruginosa isolates, five isolates from 42 S. maltophilia contain the AIM\u0000gene with a percentage (11.9%), based on the Polymerase chain reactions assay.\u0000Keywords: Stenotrophomonas maltophilia, Carbapenem Resistance, Pseudomonas aerginosa.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140241437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytogenotoxic effect of trichothecene T2 toxin on Allium sativum root tip meristematic cells","authors":"Nasreen Jalal Hussein, A. A. Saadullah","doi":"10.21931/rb/2024.09.01.45","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.45","url":null,"abstract":"Trichothecene T2 is a mycotoxin from the Fusarium species. This research aims to test the effect of the\u0000Trichothecene T2 toxin on mitotic index% (M.I.%) and induction of mitotic aberrations by using the Allium\u0000sativum (garlic) test system. The toxin concentrations in ppm were 0.00, 0.30, 0.60, 0.90, and 1.20 for 12\u0000hours. The garlic roots were then cut, and mitotic slides were prepared using squash and examined under a\u0000light microscope. The results revealed that the mycotoxin has a significant mitodepressive effect at all concentrations compared to the control, and the MI% reduction was proportional to increasing toxin concentration. The highest reduction in mitotic index was observed in the 1.2 ppm treatment.\u0000Moreover, this mycotoxin induced and increased the rate of mitotic abnormalities% (MA%) with increasing\u0000the mycotoxin concentration. The observed mitotic abnormalities were star-shaped anaphase, sticky metaphase, C-mitosis, sticky anaphase, depolarization, micronuclei, laggard chromosomes, anaphase bridges, and\u0000chromosome loss. The least frequently observed abnormality was micronuclei compared to the most frequent\u0000aberration, laggard chromosomes. The total mitotic abnormalities significantly increased with increasing the\u0000toxin dose concentration. These results suggest that this mycotoxin can inhibit the mitotic activity of the meristematic cells; it is mutagenic and can disrupt the spindle fibers' activity of the dividing cells at all concentrations, especially at higher doses in food. Therefore, the foods must be tested for fungi producing this mycotoxin.\u0000Keywords: Mycotoxin; mitodepressive; root tip; spindle fibers; mutagenic","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of the effectiveness of the electrocoagulation process in the treatment of leachate from the controlled landfill site in Francisco de Orellana canton","authors":"Cristian Cumbicus, Mayra Hurtado, Julio Coello, Pedro Peñafiel-Arcos, J. Orejuela-Romero, Marcos Barahona","doi":"10.21931/rb/2024.09.01.19","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.19","url":null,"abstract":"The inadequate management of leachate produced in landfill sites, sanitary dumps, or its incomplete treatment generates significant environmental and public health impacts. These conditions are expected in developing countries and are a major concern, especially in sensitive areas like the Amazon. This study investigated the efficiency of electrocoagulation for removing BOD5, COD, TSS, turbidity, and color using a laboratory-scale reactor. Samples of raw leachate from the controlled landfill site in Francisco de Orellana canton, located in the Ecuadorian Amazon, were used. First, the initial conditions of the leachate were determined through a physicochemical characterization, where a reduced presence of heavy metals and high biodegradability were identified, suggesting that it is old leachate. In turn, a reactor with 5 electrodes was installed, where aluminum was used as a cathode and iron as an anode. Finally, electrocoagulation was employed with various operational combinations, where a run using 2.5 V and 20 minutes showed the highest removal efficiency on average, with reductions of 85.23% of BOD5, 98.20% of COD, 11.30% of TSS, 96.52% of turbidity, and 90.73% of color. \u0000 \u0000Keywords: Electrocoagulation; Leachate; Leachate treatment.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluación de parámetros de calidad de naranja (Citrus × sinensis) en tres estados de madurez","authors":"J. Villares, Mariela Cobos, L. Vásquez, R. Sarmiento, Fanny Maita, C. Freire, Lascano Alexis, Jermis Alvarado","doi":"10.21931/rb/2024.09.01.10","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.10","url":null,"abstract":"En la Amazonía ecuatoriana los frutales son parte de la biodiversidad de la chakra y los cítricos es uno de los grupos relevantes dado que tienen un sin número de usos y cuyos excedentes son usados para la comercia-lización en las ferias locales, por lo que está investigación busca evaluar naranjas en tres estados de madurez mediante análisis físicos para la determinación del tiempo de cosecha. Se evaluaron los parámetros de calidad de la naranja (Citrus × síntesis), en tres estados de madurez (inicial-medio-completo), se determinó: peso, morfometría, color, firmeza y sólidos solubles. Las frutas se obtuvieron en el cantón Joya de los Sachas, se recolectaron frutas libres de defectos considerables. Como resultado se obtuvo que la naranja pierde peso a medida que transcurren los días. El diámetro se reduce desde el día 1 al 10 y a partir del día 7 la pérdida de longitud es igual en los tres estados de madurez. La luminosidad es el valor del color que sufre mayores cambios a lo largo del almacenamiento de la fruta. La firmeza disminuye más a partir del día 7. A medida que el estado de madurez avanza mayor será la cantidad de sólidos solubles. El estado de madurez perfecto es el medio, ya que ofrece un equilibrio entre alto contenido de azúcar y un peso y tamaño adecuados, beneficiando tanto al agricultor como al cliente debido a su dulzura.\u0000 \u0000Palabras clave: cítricos, Amazonía, Ecuador, calidad, frutas, postcosecha","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Five Concentrations of Aqueous Extracts of Pleurotus ostreatus P. Kumm and Tagetes minuta L. on the Mortality of Two Nematodes in a Laboratory Setting","authors":"Madison Chango, Gabriela Rosero, Norma Erazo, Pablo Álvarez","doi":"10.21931/rb/2024.09.01.9","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.9","url":null,"abstract":"The nematode attacks affect several plant species of Ecuadorian crops. There are fungi and plants with nematicide ability that have agricultural interest. This study assessed the effect of five concentrations of aqueous extracts of Pleurotus ostreatus and Tagetes minuta on the mortality of Meloidogyne spp. and Panagrellus redivivus nematodes in a laboratory setting. The aqueous extracts were obtained through hydro distillation where concentrations of 0%, 0.5%, 5%, 25%, 50% and 100% were prepared. A wholly randomized single-factor design was used for the P. ostreatus extract and a bifactorial for the T. minuta extract (leaves and flowers). The number of dead individuals was evaluated, and the efficacy and LC50 were determined. T. minuta leaf extract showcased higher nematicide activity against P. redivivus with an LC50 of 8.03 ppm; when applied to Meloidogyne sp., the extract showed nematicide activity with an LC50 of 0.01 ppm. For P. ostreatus extract, the greatest nematicide activity against P. redivivus was an LC50 of 1.22 ppm and nematicide activity against Meloidogyne sp., was an LC50 of 0.01 ppm. The aqueous extract of T. minuta flowers showed low nematicide activity and the aqueous extract of T. minuta leaf showed the best nematicide activity.\u0000 \u0000Keywords: nematicide; Tagetes minuta; Pleurotus ostreatus; Panagrellus redivivus; Meloidogyne sp.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological nematicides as an alternative for control of Meloidogyne incognita populations in yellow pitahaya (Sselenicereus megalanthus).","authors":"Yadira Vargas, Jimmy Pico, Neiver Manobanda, Angel Garcia, Jessica Sanmiguel","doi":"10.21931/rb/2024.09.01.4","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.4","url":null,"abstract":"Yellow pitahaya in the Ecuadorian Amazon has become one of the most important economic crops in the region. However, the presence of pests (nematodes) in the soil has caused up to 100% of the crop's growth stages to be affected. Faced with this problem, growers use various chemical nematicides that minimize this impact but cause contamination problems. For this reason, the objective of the research was to evaluate microorganisms that control or reduce the population of Meloidogyne incognita in the pitahaya crop at the greenhouse level. The design was DBCA, and the statistical analysis was performed with the statistical package Infostat 2017, using linear mixed models and Fisher's tests at 5%. The results show that root nodules decrease when P. lilacinum + T. asperellum is applied after nematode injection (261). In addition, the lowest number of nodulations (251) was obtained when microorganisms were applied after nematode inoculation (251 nodulations). Still, when microorganisms were used before, aerial biomass growth was stimulated (384.17 g) even when nematodes were present in the root system.\u0000 \u0000Keywords: microorganisms; nematodes; pitahaya.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estrategia de intervención educativa para el abordaje del riesgo cardiovascular en pacientes con hipertensión arterial","authors":"Karen Rojas-Montenegro, Gustavo Cisneros-Cañas, Rosario Suárez de Terán, Ruben Cajilima-Vega, Cecilia Arteaga-Pazmiño, Evelyn Frías-Toral","doi":"10.21931/rb/2024.09.01.68","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.68","url":null,"abstract":"Introducción: Las enfermedades cardiovasculares (ECV) son la principal causa de muerte prevenible a nivel mundial y nacional. Frente a esta problemática, las estrategias educativas son esenciales en la prevención y manejo de las ECV. Objetivo: El objetivo de este estudio fue desarrollar una estrategia de intervención educativa para abordar el riesgo cardiovascular (RCV) en pacientes con hipertensión arterial (HTA). Métodos: Se realizó un estudio cuasi-experimental, en el que se determinó el RCV y hábitos de vida a través de la Escala Globorisk y FANTASTIC, respectivamente, previo a la intervención educativa y posterior a ésta, en una población de 97 pacientes de 40 a 79 años de edad con HTA primaria en el periodo de marzo a agosto del 2021. La intervención consistió en estrategias educativas para promover hábitos de vida saludables, y la prescripción de un plan de alimentación individualizado de acuerdo a las necesidades de los participantes. Resultados: Posterior a la intervención se obtuvo un control de la tensión arterial en el 28.86% de la población de estudio, el índice de masa corporal disminuyo en el 3.1%, el RCV disminuyó en un 11.34%, y los estilos de vida saludables mejoraron en un 9.15% (p=0.000) en todas las variables. Conclusiones: En los pacientes con HTA, las intervenciones educativas para promover estilos de vida saludables, mejoran los factores de RCV.\u0000Keywords: Hipertensión arterial; Riesgo cardiovascular; Estilos de vida saludables, Atención primaria en salud.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of a natural colorant powder from Syzygium Cumini L. (Skeels) on sensory and physicochemical properties during storage of a heat-treated flavored fermented milk","authors":"Fabián M. Gaibor, Daliannis Rodríguez, Mario A. García, Alicia Casariego","doi":"10.21931/rb/2024.09.01.59","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.59","url":null,"abstract":"This study aimed to evaluate the influence of a powder colorant obtained from a hydroalcoholic extract of\u0000jambolan (Syzygium cumini) on the chemical, microbiological, and sensory characteristics of heat-treated flavored fermented milk. The extraction of anthocyanins from the pulp was carried out by maceration with 90%\u0000(v/v) ethanol acidified with citric acid. This extract was concentrated (14 to 15% w/v of total solids). Maltodextrin DE 12 was added to obtain 25% (w/v) total solids. Guar gum (0.06% w/w) was added as a stabilizer\u0000to whole milk reconstituted with sterilized distilled water (11% w/v total solids). The colorant powder was\u0000homogenized at a rate of 1.5 and 2 g per 100 mL of powdered whole milk dissolved in the corresponding\u0000amount of water, and sucrose and concentrated strawberry flavoring were added. The natural colorant addition\u0000allowed us to obtain a product with pH (4.23-4.75), titratable acidity (1.28-1.47% w/w lactic acid), and color\u0000stability, similar to those of yogurt with synthetic colorants. No microbial growth or color changes were detected. The judges did not notice any strange odor, taste, or color. Natural colorants can be a beneficial option\u0000for developing healthy and sustainable foods.\u0000Keywords: Syzygium cumini; anthocyanins; spray-dried; natural colorant; heat-treated fermented milk.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diseño de un sistema de agitación para un biorreactor de 1m3 de capacidad para la producción de ácido glicérico a partir de glicerol por fermentación microbiana de Acetobacter tropicalis","authors":"Daniel Cabrera-Valle, Henry Pérez","doi":"10.21931/rb/2024.09.01.42","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.42","url":null,"abstract":"This work presents the design of an agitation system for a 1m3 capacity bioreactor intended to produce glyceric acid (GA) through microbial fermentation of A. tropicalis using 20% v/v glycerol as the primary substrate. Building on previous studies that evaluated ideal conditions for glyceric acid production, initial data were utilized to identify the impeller type and perform subsequent calculations, including impeller Reynolds number, power consumption, and mixing time. Once the agitator type was specified, standard geometric relationships were employed to size components of the agitation system, such as tank size, baffle plates, and agitator dimensions. Power consumption calculations were compared with results from other studies on fermentation media with similar rheological characteristics. The agitator's power consumption for mixing 1m3 of medium was determined to be 8.35 kW, achieving 95% homogenization in 5.14 seconds, with a mixing time error of approximately 10%, operating at an agitation speed of 500 rpm.\u0000 \u0000Keywords: agitation system; glyceryc acid; bioreactor","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevention of cocoa moniliasis using Progressive Web Applications and sensor data in the province of Francisco de Orellana","authors":"Darwin Romero, Pilar Oña, Pedro Aguilar, Wilson Chango","doi":"10.21931/rb/2024.09.01.15","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.15","url":null,"abstract":"Ecuador is an essential cocoa producer recognized for its quality and aroma. Additionally, it holds a prominent position among the country's traditional export products, making it the third-largest cocoa-producing country in the world. However, the cocoa industry faces challenges due to moniliasis, a fungal disease that affects cocoa trees and causes damage to the fruits, resulting in decreased production. This research aims to prevent cocoa moniliasis by conducting tests with different algorithms to select the best one for predicting moniliasis using sensor data in the progressive web application. Various supervised learning algorithms were applied, including PCA, IPCA, KPCA, Linear Regression, Sci-Kit Learning, and ensemble methods like Bagging and Boosting. Google's Lighthouse is utilized for artifact validation. It is concluded that the Boosting ensemble method with a value of 1.0 and 4 estimators is the algorithm that shows a good fit for prediction. In artifact validation, it yields favorable results with a score of over 90 in various Lighthouse parameters.\u0000 \u0000Keywords: Moniliasis 1; Progressive Web Application 2; PCA 3; IPCA 4; KPCA 5; Linear Regression 6; Bagging 7; Boosting 8; Lighthouse 9","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}