从纳杰夫省各种感染中分离出的嗜麦芽糖血单胞菌和耐碳青霉烯类假单胞菌中检测 bla-AIM Metallo Beta Lactamase 基因

Enas Jalil Baqer AL-Mayali, S. Al-Muhanna, Israa Abdul Ameer Al-Kraety
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引用次数: 0

摘要

嗜麦芽霉单胞菌是一种 "迅速发展的令人担忧的病原体",越来越多的人发现了这种病原体。世界卫生组织也认为它是医院中最重要的多重耐药病原体之一。此外,铜绿假单胞菌也是一种机会性人类病原体,会引起大多数与医疗相关的感染,被认为是抗生素耐药性发展的典范。在全球许多医院中,耐碳青霉烯类的铜绿假单胞菌已成为一个重要的感染源。本研究旨在研究从不同临床样本中分离和诊断嗜麦芽糖酵母菌和铜绿假单胞菌的情况,评估从临床样本中分离的铜绿假单胞菌对碳青霉烯类耐药的发生率,并调查这些分离株之间 bla-AIM 基因的传播情况。研究人员在2022年至2023年间从各种临床样本中收集了八百五十份标本。标本包括 220 份拭子(烧伤)、200 份(尿液)、140 份(粪便)和 130 份(伤口)。90份(耳)、50份(咽喉)、10份(脑脊液)和10份(血液)。其中 680 份标本含有细菌生长,170 份标本无细菌生长。在分离出的 680 株细菌中,410 株为革兰氏阴性菌,270 株为革兰氏阳性菌。在 MacConkey 琼脂中,180/410 个细菌是乳糖发酵菌;在其他分离物中,230/410 个细菌是乳糖不发酵菌。根据主要的诊断方法,然后使用 VITEK-2 紧凑型系统,以横断面的方式分离和鉴定了这一时期分离出的嗜麦芽气单胞菌和铜绿假单胞菌。结果显示,从所有革兰氏阴性菌中分离出 42 株嗜麦芽糖酵母菌和 80 株铜绿假单胞菌。结果显示,在48株耐碳青霉烯铜绿假单胞菌分离株中,只有5株分离株含有AIM基因,所占比例为(10.4%);根据聚合酶链反应检测,42株嗜麦芽汁酵母菌中有5株分离株含有AIM基因,所占比例为(11.9%):嗜麦芽汁霉菌 对碳青霉烯类耐药性 铜绿假单胞菌
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of bla-AIM Metallo Beta Lactamase Gene among Stenotrophomonas Maltophilia and Carbapenem Resistant Pseudomonas Aeruginosa Isolated from Various Infections in AL- Najaf Province
Stenotrophomonas maltophilia is a "rapidly evolving pathogen of concern" that is increasingly being identified. The World Health Organization also recognizes it as one of the hospitals' most significant multi-drugresistant pathogens. Also, Pseudomonas aeruginosa is an opportunistic human pathogen that causes most healthcare-associated infections, and it is considered a paradigm of antibiotic resistance development. In many hospitals across the globe, carbapenem-resistant Pseudomonas aeruginosa has emerged as a significant source of infection. The present study aimed to study the isolation and diagnosis of S. maltophilia and P. aeruginosa from different clinical samples, evaluate the occurrence of carbapenem resistance of P. aeruginosa isolated from clinical samples, and investigate the dissemination of the bla-AIM genes between these isolates. Eight hundred fifty specimens were collected from various clinical samples between 2022 and 2023. The specimens included 220 swabs (burn), 200 (urine), 140 (stool), and 130(wound). 90 (ear),50 (throat), 10 (Cerebrospinal fluid), and 10 (blood). Represented by 680 specimens contained bacterial growth, and 170 specimens had no bacterial growth. Out of the 680 bacterial growth isolates, 410 revealed growths of Gram-negative bacteria, and 270 were Gram-positive bacteria. On MacConkey ag, ar 180/410 bacteria were lactose ferment; other isolates, es 230/410 of the isolates were lactose non-fermented bacteria. In a cross-sectional manner, Stenotrophomonas maltophilia and Pseudomonas aeruginosa isolates during this period were isolated and identified depending on the primary methods of diagnosis, then the use of the VITEK-2 compact system. The results showed 42 isolates of S. maltophilia and 80 isolates of P. aeruginosa from total Gram-negative bacteria. The results show that only five isolates contained the AIM gene, with a percentage of (10.4 %) of the 48 Carbapenem Resistant Pseudomonas aeruginosa isolates, five isolates from 42 S. maltophilia contain the AIM gene with a percentage (11.9%), based on the Polymerase chain reactions assay. Keywords: Stenotrophomonas maltophilia, Carbapenem Resistance, Pseudomonas aerginosa.
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