Bionatura最新文献

筛选
英文 中文
HIV knowledge and preventive Standards Precautions Among Healthcare Workers in Blood Transfusion Centers 输血中心医护人员的艾滋病知识和预防性标准预防措施
Bionatura Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.44
Nasir Muwfaq Younis, Mahmoud Mohammed Ahmed, Alaa Yousif Ayed
{"title":"HIV knowledge and preventive Standards Precautions Among Healthcare Workers in Blood Transfusion Centers","authors":"Nasir Muwfaq Younis, Mahmoud Mohammed Ahmed, Alaa Yousif Ayed","doi":"10.21931/rb/2024.09.01.44","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.44","url":null,"abstract":"The number of instances of HIV has climbed considerably in the previous ten years, which is cause for alarm.\u0000Because healthcare workers are future healthcare professionals, they must have proper knowledge and preventive standards and precautions about HIV because they will play a vital role in the prevention of HIV\u0000transmission and treatment of HIV patients. The article aims to identify HIV knowledge and preventive standard precautions among healthcare workers in blood transfusion Centers. Cross-sectional descriptive research\u0000to characterize and provide information about the knowledge and preventive standards precautions among\u0000healthcare workers in blood transfusion Centers from March 25 to April 15, 2022. Participants from the described Studies (N = 34) provided data for these analyses. This result revealed that the participants' knowledge\u0000ranged between acceptable and good (47.05 and 52.95). Also, participants' knowledge of prevention strategies\u0000was similar, but the hand hygiene strategy was the most valuable (M: 3.62 and SD: 0.45). The instrument\u0000consists of 3 parts: part 1: demographical variables. Part two: healthcare workers' knowledge of HIV, Part 3:\u0000preventive standards precautions among healthcare workers. The current study of healthcare personnel shows\u0000that they have a decent understanding of HIV based on the precise results and adhere to the preventive\u0000measures during the blood transfusion.\u0000Keywords: HIV, knowledge, preventive Standards precautions, Healthcare Workers, Blood Transfusion Centers","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of injection with microelements, jasmonic and citric acid on some date palm c.v Khastawi yieldtraits 注射微量元素、茉莉酸和柠檬酸对某些椰枣产量特征的影响
Bionatura Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.53
W. Taha, Kh.A.S. Al-Hamdani, KH. N. AL-Assal
{"title":"Effect of injection with microelements, jasmonic and citric acid on some date palm c.v Khastawi yield\u0000traits","authors":"W. Taha, Kh.A.S. Al-Hamdani, KH. N. AL-Assal","doi":"10.21931/rb/2024.09.01.53","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.53","url":null,"abstract":"The study was conducted at the Ishaqi palm plant affiliated with the Department of Horticulture/Ministry of\u0000Agriculture during the growing season of 2021 on date palm trees of the Khastawi variety to study the response\u0000of the yield traits of the Khastawi date palm trees to the injection of microelements, jasmonic and citric acid.\u0000The study included three factors: The first factor is the injection of microelements with three treatments: injection with water only, symbolized by D0, iron injection at a concentration of 300 mg, and iron sulfate. Palm1\u0000, symbolized by D1, and boron injection through boric acid at a concentration of 40 mg Palm-1\u0000, symbolized\u0000by D2. The second factor is the treatment of injections with the Jasmonic growth regulator, which was injected\u0000three days after the injection with the microelements and with the same number of additions (0, 50, 100)\u0000micrograms. L-1 is symbolized by G0, G1, and G2 sequentially. The trees were injected with the microelements\u0000and the Jasmonic growth regulator before Immunization, after vaccination in 20 days, and after 40 days. The\u0000third factor was injection with citric acid and included three levels (0, 500, 1000) mg l-1 symbolized by S0, S1,\u0000and S2 sequentially. The results showed that the D2 treatment was significantly superior in the average diameter, length, size and weight of the fruit and the total yield, as it reached 24.86 mm, 3.46 cm, 11.10 cm, 10.07\u0000g, 36.00 kg.palm-1 sequentially, while the comparison treatment gave the lowest average for the traits. As for\u0000the effect of jasmonic acid, the injection treatment was superior to (100 µg L-1\u0000). (G2) gave the highest average\u0000for the diameter, length, size, weight of the fruit, and the total yield, which reached 25.90 mm, 3.63 cm, 12.09\u0000cm, 10.93 g, and 35.96 kg. Palm-1, respectively, while the comparison treatment gave the lowest average for\u0000the traits above as for the effect of citric acid. The treatment exceeded 1000 mg.L-1 (S2) gave the highest rate\u0000in the diameter, length, size, and weight of the fruit, and the total yield, reaching 24.84 mm, 3.49 cm, 11.13\u0000cm, 9.04 g, and 36.30 kg. Palm-1, respectively, while the comparison treatment gave the lowest average for\u0000the traits above, as the two- and three-interactions showed. The research factors have significant differences\u0000for all the studied traits.\u0000Keywords: Trace elements, jasmonic acid, citric acid, Khastawi variety.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Root-shoot ratio and its relationships with physiological characteristics, growth and biomass yield ofGynura procumbens under different shade levels and plant density 不同遮荫水平和植株密度下根-芽比及其与玄参生理特征、生长和生物量产量的关系
Bionatura Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.52
Omar Ali Ahmed, Martini Mohammad Yusoff, A. Misran, Puteri Edaroyati Megat Wahab, Qusay Abdualhamza Muttaleb
{"title":"Root-shoot ratio and its relationships with physiological characteristics, growth and biomass yield of\u0000Gynura procumbens under different shade levels and plant density","authors":"Omar Ali Ahmed, Martini Mohammad Yusoff, A. Misran, Puteri Edaroyati Megat Wahab, Qusay Abdualhamza Muttaleb","doi":"10.21931/rb/2024.09.01.52","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.52","url":null,"abstract":"Gynura procumbens is one of the most common medicinal plants in the Asteraceae family, with extensive\u0000pharmacological properties. The experiment was conducted to evaluate the effects of different shade levels (0\u0000and 30% shade) and plant density (9, 15, and 25 plants m-2) on root-shoot ratio and it is a relationship with\u0000physiology, growth, and biomass yield using split-plot design with three replications. Increasing shade level\u0000to 30% shade significantly decreased root-shoot ratio (RSR) by22.54%, while total leaf dry weight per plant\u0000(TLDW) and total leaf dry weight per square meter (TLDW m-2) increased by 35.64, 11.58, and 32.18%,\u0000respectively due to negative correlation with RSR. Increasing plant density from 9 to 25 plants m-2 significantly increased RSR and TLDW m-2 by 67.71 and 18.54%, respectively, while TLDW decreased by 57.31%.\u0000There was a negative correlation between RSR and biomass yield per plant. Under stressed conditions (full\u0000sunlight and high plant density), G. procumbent plants appeared to change strategy to absorb limited resources,\u0000allocate more biomass to the root system, and reduce aboveground parts' size to survive, resulting in high\u0000RSR.\u0000Keywords: Gynura procumbens, shade, plant density, root-shoot ratio, physiology, growth, biomass","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Coexistencia de procesos productivos y de conservación en la Amazonía ecuatoriana 厄瓜多尔亚马逊河流域生产与保护进程的共存
Bionatura Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.1
Maritza Sánchez-Capa, Hilda Fabiola Anguaya Isama
{"title":"Coexistencia de procesos productivos y de conservación en la Amazonía ecuatoriana","authors":"Maritza Sánchez-Capa, Hilda Fabiola Anguaya Isama","doi":"10.21931/rb/2024.09.01.1","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.1","url":null,"abstract":"La región amazónica de Ecuador está conforma por las provincias Sucumbíos, Orellana, Napo, Pastaza, Morona Santiago y Zamora Chinchipe, su extensión es de ~120.000 Km2 1. En las dos últimas décadas, la educación superior se ha implementado en cada una de las provincias, buscando responder a las necesidades de profesionales en los diferentes sectores que constituyen las actividades económicas principales de cada provincia. Sucumbíos y Orellana son provincias que sustentan su economía en la producción petrolera, sin embargo, en esta superficie se encuentran áreas protegidas como la reserva de la biósfera YASUNÍ, parque nacional Cuyabeno, reserva Limoncocha, que son áreas con una gran biodiversidad por lo que la coexistencia entre conservación y producción industrial generan una búsqueda consante de alternativas sustentables y sostenibles","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Predictive Model in Production through Progressive Web Applications to Forecast Moniliasis in Cacao. 通过渐进式网络应用程序建立生产中的预测模型,以预测可可中的莫尼里斯病。
Bionatura Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.14
Aracely Miranda, Byron Bonifaz, Wilson Chango, Pedro Aguilar
{"title":"Predictive Model in Production through Progressive Web Applications to Forecast Moniliasis in Cacao.","authors":"Aracely Miranda, Byron Bonifaz, Wilson Chango, Pedro Aguilar","doi":"10.21931/rb/2024.09.01.14","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.14","url":null,"abstract":"Cocoa is considered a significant crop in Ecuador, as it represents a favorable source of income for the country's economy, thanks to the remarkable quality of the product. However, it faces a significant issue in its crops: moniliasis, a fungal disease that attacks cocoa cultivation, is present in most Latin American countries. Consequently, this leads to decreased cocoa production and a lower final product quality. The study focuses on designing a predictive production model through a progressive web application to forecast moniliasis in cocoa. The objective is to create an application that anticipates the presence of this disease, thereby contributing to the improvement of the local economy for all farmers. Various methodologies were employed, including bibliographic methods, design science research methodology, and machine learning models. The results obtained from this research indicate that the Gradient Boosting Classifier is the algorithm that best fits the provided dataset. Once this algorithm was identified, a progressive web application was developed and made available for public use by farmers. Furthermore, the efficiency of the predictive model was verified using the statistical method of central tendency, demonstrating that the predictive model is beneficial, primarily by saving farmers a significant amount of time. Anticipating the disease enables timely preventive and corrective measures, which could reduce losses in cocoa production and enhance the quality of the final product.\u0000 \u0000Keywords: cacao, moniliasis, predictive model, progressive web apps, supervised learning.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140241233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heart failure prognostic algorithm using Spectral Analysis and MATLAB software. 使用频谱分析和 MATLAB 软件的心力衰竭预后算法。
Bionatura Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.12
Segundo Fabián Siza Moposita, Miguel Ángel Sáez Paguay, Yesenia Maricela Fiallos Godoy, Ricardo Fabián Siza Gualpa
{"title":"Heart failure prognostic algorithm using Spectral Analysis and MATLAB software.","authors":"Segundo Fabián Siza Moposita, Miguel Ángel Sáez Paguay, Yesenia Maricela Fiallos Godoy, Ricardo Fabián Siza Gualpa","doi":"10.21931/rb/2024.09.01.12","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.12","url":null,"abstract":"Fourier analysis for biological signals is based on the use of the infinite sum of sines and cosines that allows modeling: the periodic functioning of the heart, its amplitude, frequency, and phase period, transforming these signals into images called ECG, based on studies and programs that model the ideal functioning of the heart. In this work, a mathematical algorithm has been designed to predict the cardiac pathology called bradycardia, which relates the prolongation of the monthly QT interval in the order of 10-4 seconds/month in a time of 10 years with the ventricular alteration. MATLAB software and spectral analysis are used to contrast the spectrum without pathology, which contains harmonics of greater amplitude, with a spectrum already with pathology that reaches heart failure, where the most significant number of harmonics are grouped in the first values, and then the model with an exponential function the delay of the QT interval in the ECG, concluding that up to 40 months after the onset of the pathology, the patient can counteract the disease. In comparison, by 80 months, difficulties arise, even the disease becomes irreversible in the last months, and the blood-propelling organ ceases to function.\u0000\u0000Keywords: Heart Failure Prognostic, Bradycardia, Fourier Series, Spectral Analysis.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140391722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Análisis multitemporal de deforestación y cambio de la cobertura del suelo, en el cantón La Joya de los Sachas, período 1990-2018. 1990-2018 年期间拉霍亚德洛斯萨夏斯州森林砍伐和土地覆被变化的多时空分析。
Bionatura Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.18
Fernando Paredes-Arcos, Leider Tinoco-Jaramillo, Carlos D. Congo-Yépez, Yadira Vargas-Tierras
{"title":"Análisis multitemporal de deforestación y cambio de la cobertura del suelo, en el cantón La Joya de los Sachas, período 1990-2018.","authors":"Fernando Paredes-Arcos, Leider Tinoco-Jaramillo, Carlos D. Congo-Yépez, Yadira Vargas-Tierras","doi":"10.21931/rb/2024.09.01.18","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.18","url":null,"abstract":"La Joya de los Sachas, es uno de los principales cantones productores de petróleo y productos agrícolas; sin embargo, el incremento de la frontera agrícola ha ocasionado cambios importantes en la cobertura y uso del suelo. Ante esta situación, el estudio tuvo la finalidad de conocer las causas y efectos que ha ocasionado el cambio de cobertura para obtener las tasas anuales e índices de cambio de cobertura. El análisis se realizó mediante el geoprocesamiento de información geográfica disponible en el portal del Ministerio de Ambiente Agua y Transición Ecológica (MAATE) y el Instituto Geográfico Militar (IGM), se utilizó el método de tablas de tabulación cruzada y sobreposición de capas para determinar el contraste de las coberturas entre las fechas establecidas. Los resultados muestran un incremento acumulado de 17.626,5 ha de tierra agrícola y 16.767,6 ha de pasto en los últimos 28 años; las actividades agropecuarias se han convertido en la principal causa de deforestación. Durante el período 1990-2018 se perdieron 36.413 ha de bosques, con una tasa anual de deforestación de 1.300 ha*año-1. Este comportamiento permitió estimar si la tendencia persiste, en 30 años aproximadamente La Joya de los Sachas perderá completamente el bosque. Además, las políticas públicas, dolarización, apertura de caminos y cambios en la actividad agrícola causaron impactos en las coberturas y usos de suelo durante los tres periodos evaluados (1990-2000, 2000-2008 y 2008-2018).\u0000 \u0000Palabras Clave: Suelo, Uso de suelo, cobertura, tierra agrícola, pastizal, bosque, mapas.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ameliorative Effect of Cinnamon and Rosemary Oils in Acrylamide–Induced Hepatic Injury in Rats 肉桂和迷迭香油对丙烯酰胺诱导的大鼠肝损伤的改善作用
Bionatura Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.58
Hala Elsayed, Ashraf Abd El-Hakim El komy, Elham Abd-El Moneim El-Shewy, Faten Ebrahim Elsayed Abdallah
{"title":"Ameliorative Effect of Cinnamon and Rosemary Oils in Acrylamide–Induced Hepatic Injury in Rats","authors":"Hala Elsayed, Ashraf Abd El-Hakim El komy, Elham Abd-El Moneim El-Shewy, Faten Ebrahim Elsayed Abdallah","doi":"10.21931/rb/2024.09.01.58","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.58","url":null,"abstract":"Liver diseases can result from various causes, such as viruses, bacteria, autoimmune disorders, or certain\u0000medications and toxic substances. While modern medicine offers treatments for these conditions, there needs\u0000to be more effective drugs that can protect and regenerate liver cells. Therefore, it is crucial to identify new\u0000treatment options and liver-protective agents that are both highly efficient and safe. This study is assigned to\u0000investigate the adverse effects of acrylamide on the liver in rats and explore whether these effects can be\u0000mitigated by co-administration of cinnamon oil (C.O.), rosemary oil (R.O.), or a combination of both oils\u0000during acrylamide exposure. A total of 70 male albino rats were divided randomly into 7 groups, each group\u0000of 10 rats, that received different treatments: control group, acrylamide-treated group (20 mg/kg b.wt), cinnamon oil-treated group (200 mg/kg b.wt), rosemary oil-treated group (250 mg/kg b.wt), acrylamide and cinnamon oil-treated group, acrylamide and rosemary oil-treated group, and acrylamide, cinnamon oil, and Rosemary oil-treated group. These treatments were administered orally for 28 consecutive days. Blood and liver\u0000tissue samples were gathered at the end of the study to assess the outcomes. The results revealed that cinnamon\u0000oil and rosemary oils exhibited hepatoprotective effects, as evidenced by normalized liver function parameters\u0000(alanine transaminase, Aspartate transaminase, and Alkaline phosphatase), as well as improvements in nonenzymatic parameters (total protein, albumin, cholesterol, triglyceride, low-density lipoprotein, and high-density lipoprotein). The observed hepatoprotection of cinnamon oil and rosemary oils was attributed to their\u0000ability to reduce oxidative stress caused by acrylamide, as demonstrated by lower levels of liver cell lipid\u0000peroxidation product (malondialdehyde) and enhanced activity of antioxidative enzymes (glutathione and catalase) in liver tissue.\u0000Keywords: Cinnamon, Rosemary, Acrylamide, Liver, Rats, Antioxidants","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro evaluation of the inhibitory capacity of three Trichoderma isolates on Ralstonia solanacearum 体外评估三种毛霉分离菌对茄枯镰孢菌的抑制能力
Bionatura Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.6
Jimmy Trinidad Pico Rosado, Christopher Wilson Suárez Palacios, Jessenia Jiménez Cumbicus, Ernesto Paredes Puga, Gladys Sabando, Liliana Andrade Olalla
{"title":"In vitro evaluation of the inhibitory capacity of three Trichoderma isolates on Ralstonia solanacearum","authors":"Jimmy Trinidad Pico Rosado, Christopher Wilson Suárez Palacios, Jessenia Jiménez Cumbicus, Ernesto Paredes Puga, Gladys Sabando, Liliana Andrade Olalla","doi":"10.21931/rb/2024.09.01.6","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.6","url":null,"abstract":"Bacterial wilt in bananas, caused by Ralstonia solanacearum or Moko, limits crop production and threatens Ecuador. This study evaluated Trichoderma isolates in laboratory conditions as an innovative alternative to ensure sustainability in banana production. The four R. solanacearum. isolates were obtained from banana plants exhibiting disease symptoms and were characterized through morphological and biochemical tests. Four treatments were evaluated: three isolates of fungi from the genus Trichoderma (Trichoderma viride, T. harzianum, T. asperellum) and one consisting of a combination of the three isolates above. The inhibitory capacity of the Trichoderma isolates on R. solanacearum colonies was measured. A completely randomized design with three replicates was used, and general linear and mixed models were employed, with qq-plot graphs for normality and residual plots for variance homogeneity.\u0000 \u0000Furthermore, a Fisher's LSD test was conducted at a significance level of α = 0.05. In the biochemical tests, the bacterial isolates exhibited specific characteristics of R. solanacearum in two bacterial isolates. In the inhibition tests, treatment four and treatment one (consortium of the three Trichoderma isolates and Trichoderma viride) showed the highest inhibitory potential, with 76.07% and 61.19%, respectively. The consortium of Trichoderma isolates demonstrated the highest inhibitory potential against R. solanacearum, with day 10 being the time with the highest percentage of inhibition (72.61%).\u0000 \u0000Keywords: Bacterial wilt, Ralstonia solanacearum, Trichoderma, inhibition","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Analysis of Threshold Cycle Results for RNA Extraction in SARS-CoV-2 RT-qPCR Using Magnetic Beads and Spin Column Methods 使用磁珠法和旋转柱法提取 SARS-CoV-2 RT-qPCR 中 RNA 的阈值循环结果比较分析
Bionatura Pub Date : 2024-03-15 DOI: 10.21931/rb/2024.09.01.64
Fardiah Tilawati Sitanggang, James Perdinan Simanjuntak, Nasrah Nasrah, Ridwansyah Ridwansyah, Arvida Bar
{"title":"Comparative Analysis of Threshold Cycle Results for RNA Extraction in SARS-CoV-2 RT-qPCR Using Magnetic Beads and Spin Column Methods","authors":"Fardiah Tilawati Sitanggang, James Perdinan Simanjuntak, Nasrah Nasrah, Ridwansyah Ridwansyah, Arvida Bar","doi":"10.21931/rb/2024.09.01.64","DOIUrl":"https://doi.org/10.21931/rb/2024.09.01.64","url":null,"abstract":"Coronavirus Disease-2019 (COVID-19) belongs to the large family of SARS-CoV viruses, initially emerging\u0000in 2002-2003. In humans, this virus triggers respiratory infectious diseases. COVID-19, a new variant of\u0000SARS-CoV, was identified in humans following an unprecedented incident in Wuhan, China, in December\u00002019. This virus typically manifests mild symptoms, including a runny nose, sore throat, cough, and fever.\u0000The Nucleic Acid Amplification Test (NAAT), specifically the realtime Reverse Transcription Polymerase\u0000Chain Reaction (rRT-PCR) examination, is recommended by the World Health Organization (WHO) for diagnosing COVID-19. This study assessed potential differences in Threshold Cycle results during RNA extraction using magnetic beads compared to spin columns in the SARS-CoV-2 rRT-PCR method. The population for this study was selected through accidental sampling from nasopharyngeal and oropharyngeal swabs\u0000of COVID-19 patients obtained between December 2022 and April 2023, with Threshold Cycle values\u0000<30,000. The samples were stored at -80°C. The findings revealed that the average N (VIC) was 23.359, and\u0000RdRP (FAM) was 25.558 in the Magnetic Beads method, indicating a lower value compared to the average N\u0000(VIC) of 29.200 and RdRP (FAM) of 29.661 in the Spin Column method. This suggests that the Magnetic\u0000Beads method exhibited greater sensitivity than the Spin Column method. The statistical analysis confirmed\u0000these differences, with a P value of 0.003 in N (VIC) and the P value of 0.000 in RdRP (FAM). Consequently,\u0000it can be concluded that there is a significant 19.5% difference in the Threshold Cycle during RNA extraction\u0000using Magnetic Beads and Spin Column in the examination of the SARS-CoV-2 rRT-PCR method.\u0000Keywords: Sars-CoV-2; rRT-PCR; Magnetic Beads; Spin Column; Threshold Cycle.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信