Fetal and Pediatric Pathology最新文献_第5页

Performance Validation of the NeoBase 2 Non-Derivatized MSMS Assay Kit and Cutoff Values Establishment of Term and Preterm Neonates. NeoBase 2 非衍生化 MSMS 检测试剂盒的性能验证及足月儿和早产儿临界值的确定

IF 0.7 4区医学

Fetal and Pediatric Pathology Pub Date : 2024-09-01 Epub Date: 2024-08-06 DOI: 10.1080/15513815.2024.2386659

Zhihui Wan, Wei Liu, Yanhong Zhai, Zhijun Ma, Zheng Cao

{"title":"Performance Validation of the NeoBase 2 Non-Derivatized MSMS Assay Kit and Cutoff Values Establishment of Term and Preterm Neonates.","authors":"Zhihui Wan, Wei Liu, Yanhong Zhai, Zhijun Ma, Zheng Cao","doi":"10.1080/15513815.2024.2386659","DOIUrl":"10.1080/15513815.2024.2386659","url":null,"abstract":"Objective: NeoBase 2 Non-derivatized MSMS assay kit (NeoBase 2 kit) was used for newborn screening, the performance of the NeoBase 2 kit should be validated before its implementation in clinical diagnostic laboratories.Methods: Leftover dried blood spot samples, quality control materials in the NeoBase 2 kit, and proficiency testing materials received from the NSQAP were used. Precision, accuracy, LOD, LLOQ, recovery, and stability were carried out to verify the performance of the Waters ACQUITY TQD MS/MS system with the NeoBase 2 kit for newborn screening. Cutoffs were determined and analytes requiring different cutoffs in preterm neonates were investigated.Results: Within-run and between-run precisions ranged from 3.95% to 14.41%. The accuracy and stability were within 15%. All analytes demonstrated acceptable LOD, LLOQ, and recoveries. Cutoffs for term and preterm neonates were established.Conclusions: The performance of the NeoBase 2 kit is acceptable and can be implemented in clinical diagnostic laboratories.","PeriodicalId":50452,"journal":{"name":"Fetal and Pediatric Pathology","volume":" ","pages":"366-375"},"PeriodicalIF":0.7,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AKT1 and MAPK8: New Targets for Gestational Diabetes Mellitus? AKT1 和 MAPK8：妊娠糖尿病的新靶点？

IF 0.7 4区医学

Fetal and Pediatric Pathology Pub Date : 2024-08-23 DOI: 10.1080/15513815.2024.2393357

Aysegul Turkyilmaz, Melike Nur Akin, Burcu Kasap, Cilem Ozdemİr, Aysegul Demirtas Bilgic, Tuba Gokdogan Edgunlu

{"title":"AKT1 and MAPK8: New Targets for Gestational Diabetes Mellitus?","authors":"Aysegul Turkyilmaz, Melike Nur Akin, Burcu Kasap, Cilem Ozdemİr, Aysegul Demirtas Bilgic, Tuba Gokdogan Edgunlu","doi":"10.1080/15513815.2024.2393357","DOIUrl":"https://doi.org/10.1080/15513815.2024.2393357","url":null,"abstract":"Objective: Gestational diabetes mellitus (GDM) disrupts placental function and increases risks for pregnancy. This study investigates the potential involvement of AKT1 and MAPK8 genes, known for their roles in insulin resistance and cell signaling, in GDM pathophysiology. Methods: Placental tissues from GDM patients and healthy controls were analyzed using real-time PCR to quantify gene expression levels. In silico analysis further explored the functional implications of expression changes. Results: AKT1 and MAPK8 displayed significantly altered expression in GDM placentas compared to controls (p = 0.047 and p = 0.007, respectively). In silico analysis suggests potential functional consequences related to diabetes-associated pathways. Conclusion: This study identifies differential expression of AKT1 and MAPK8 in GDM placentas, suggesting their potential roles in the disease process. Further investigation into their functional contributions could provide valuable insights into GDM pathophysiology and potential therapeutic targets.","PeriodicalId":50452,"journal":{"name":"Fetal and Pediatric Pathology","volume":" ","pages":"1-9"},"PeriodicalIF":0.7,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142037599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Malformed Perinatal Anthropological Crania Contribute to New Insight in the Extension of Bone Malformations in Cranial Development. 人类畸形围产期人类学颅骨为骨骼畸形在颅骨发育中的扩展提供了新的见解。

IF 0.7 4区医学

Fetal and Pediatric Pathology Pub Date : 2024-07-01 Epub Date: 2024-05-30 DOI: 10.1080/15513815.2024.2338434

Inger Kjær, Amberley Marin, Ion Meyer

{"title":"Human Malformed Perinatal Anthropological Crania Contribute to New Insight in the Extension of Bone Malformations in Cranial Development.","authors":"Inger Kjær, Amberley Marin, Ion Meyer","doi":"10.1080/15513815.2024.2338434","DOIUrl":"10.1080/15513815.2024.2338434","url":null,"abstract":"Introduction: We describe five abnormal crania which may provide more diagnostic data for assessment of abnormal crania in newborns.Methods: Five malformed perinatal crania from the Saxtorphian Collection are described using published prenatal abnormal cranial development criteria. These malformations were compared to normal cranial development arising from the migration of neural crest cells. Visual and photographic investigations were performed.Results: The malformed crania were occipital encephalocele, holoprosencephaly, anencephaly, and two without a recognizable diagnosis. The anthropological crania were malformed in the same regions as formerly observed in fetal pathology. These regions were comparable to fields formed during normal cell migration from the neural crest. This has seemingly not previously been demonstrated. One undiagnosed cranium may represent a Treacher Collins syndrome (Case 3). The other undiagnosed cranium (Case 4) could be from a scaphocephalic specimen.Discussion: Sharp borderlines between malformed and non-malformed regions in cranial syndromes may enable improvement in diagnostics.","PeriodicalId":50452,"journal":{"name":"Fetal and Pediatric Pathology","volume":" ","pages":"277-289"},"PeriodicalIF":0.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survey Data and Experience from a Pediatric Pathology Workshop in Indonesia: Understanding Practice Needs and Utility of Outreach Teachings. 印度尼西亚儿科病理学研讨会的调查数据和经验：了解实践需求和推广教学的效用。

IF 0.7 4区医学

Fetal and Pediatric Pathology Pub Date : 2024-07-01 Epub Date: 2024-07-11 DOI: 10.1080/15513815.2024.2377651

Juan Putra, Nur Rahadiani, Chrystalle Katte Carreon

{"title":"Survey Data and Experience from a Pediatric Pathology Workshop in Indonesia: Understanding Practice Needs and Utility of Outreach Teachings.","authors":"Juan Putra, Nur Rahadiani, Chrystalle Katte Carreon","doi":"10.1080/15513815.2024.2377651","DOIUrl":"10.1080/15513815.2024.2377651","url":null,"abstract":"Objective: We aimed to share the post-workshop survey results of a pediatric pathology course held in Jakarta, Indonesia. Methods: Questionnaires were distributed to participants; responses from practicing pathologists and pathologists-in-training were analyzed. Results: The respondents (107 pathologists of 143 attendees) were predominantly female (83.2%) and 31-60 years of age (77.5%). Over half (71.7%) signed out pediatric and perinatal specimens but only a third (34.3%) were comfortable handling such cases. Most (70.0%) felt that their exposure to pediatric and perinatal cases during their training was inadequate. All respondents thought that the workshop was helpful, and would highly recommend it to their colleagues. Post-workshop, the respondents claimed expansion of differential diagnoses (49.5%) and better understanding of what to include in pathology reports (41.1%). Conclusions: Our experience affirms the need for subspecialty courses to address training gaps in developing countries. Post-workshop surveys are helpful in determining actionable deficiencies and effectiveness of outreach teachings.","PeriodicalId":50452,"journal":{"name":"Fetal and Pediatric Pathology","volume":" ","pages":"330-340"},"PeriodicalIF":0.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of E-Cadherin and Ber-EP4 in the Trophoblastic Tissues of Intrauterine and Ectopic Tubal Pregnancies. 宫内和异位输卵管妊娠滋养细胞组织中 E-Cadherin 和 Ber-EP4 的表达

IF 0.7 4区医学

Fetal and Pediatric Pathology Pub Date : 2024-07-01 Epub Date: 2024-06-24 DOI: 10.1080/15513815.2024.2368579

Nermin Koc, Sevcan Arzu Arinkan, Cansu Sonmez, Berker Kaya

{"title":"Expression of E-Cadherin and Ber-EP4 in the Trophoblastic Tissues of Intrauterine and Ectopic Tubal Pregnancies.","authors":"Nermin Koc, Sevcan Arzu Arinkan, Cansu Sonmez, Berker Kaya","doi":"10.1080/15513815.2024.2368579","DOIUrl":"10.1080/15513815.2024.2368579","url":null,"abstract":"Introduction: We investigated the role of E-cadherin and Ber-EP4 in tubal pregnancy by comparing their expressions in epithelial and trophoblastic cells both in ectopic tubal and intrauterine pregnancies. Methods: The Formalin-fixed paraffin embedded blocks of 17 intrauterine and 17 tubal pregnancies were immunohistochemically stained with E-cadherin and Ber-EP4. Results: E-cadherin was expressed in the epithelium, villous and extravillous trophoblast in tubal and intrauterine pregnancies but not in the syncytiotrophoblast. The staining intensity was lower in the extra-villous trophoblast in tubal ectopic pregnancies compared with intrauterine pregnancies. Ber-EP4 was expressed in the epithelium of tubal and intrauterine pregnancies and only in villous cytotrophoblast. The intensity of staining in tubal pregnancy was higher than in intrauterine pregnancy. Discussion: The loss of E-cadherin expression in extra-villous trophoblast and increased expression of Ber-EP4 in the villous cytotrophoblast may play a role in the formation of tubal pregnancy by allowing the blastocyst to attach to the tubal epithelium.","PeriodicalId":50452,"journal":{"name":"Fetal and Pediatric Pathology","volume":" ","pages":"290-299"},"PeriodicalIF":0.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Markers of Acute Childhood B-Lineage Lymphoblastic Leukemia in the Kazakh Population. 哈萨克人群中急性儿童 B 系淋巴细胞白血病的遗传标记。

IF 0.7 4区医学

Fetal and Pediatric Pathology Pub Date : 2024-07-01 Epub Date: 2024-07-11 DOI: 10.1080/15513815.2024.2375523

Gulnara Svyatova, Galina Berezina, Aigul Bazarbayeva, Kulyan Omarova, Abay Kussainov

引用次数: 0

Role of Telomere Length in Radiation Response of Hematopoietic Stem & Progenitor Cells in Newborns. 端粒长度在新生儿造血干细胞和祖细胞辐射反应中的作用

IF 0.7 4区医学

Fetal and Pediatric Pathology Pub Date : 2024-07-01 Epub Date: 2024-08-06 DOI: 10.1080/15513815.2024.2381752

Angshuman Biswas, Mandar Bhattacharya, Priyanka Ghosh, Subrata Kumar Dey

{"title":"Role of Telomere Length in Radiation Response of Hematopoietic Stem & Progenitor Cells in Newborns.","authors":"Angshuman Biswas, Mandar Bhattacharya, Priyanka Ghosh, Subrata Kumar Dey","doi":"10.1080/15513815.2024.2381752","DOIUrl":"10.1080/15513815.2024.2381752","url":null,"abstract":"Objective: Wide inter-individual variations in ionizing radiation (IR) responses of neonatal hematopoietic system calls for identifying reliable biomarkers to effectively estimate radiation exposure damages in neonates.Methods: Association between telomere length (TL) at birth and radiation sensitivity of cord blood hematopoietic stem cells (HSC) from 166 healthy newborns were investigated by assessing their clonogenic differentiation. TL was determined as terminal restriction fragment (TRF) by Southern blot method.Results: TL correlated with surviving fractions of total progenitor colony forming cell (CFC) content at 0.75 Gy (p < 0.05), granulo-macrophagic lineage colony forming units (CFU-GM) at 0.75 Gy (p < 0.05) and erythroid burst forming unit (BFU-E) at 0.75 Gy (p < 0.05) & at 3 Gy (p < 0.05) of newborns.Conclusion: Our results indicate risks for HSC clonogenic survival in neonates with shorter telomeres after IR exposure. These observations might aid in considering TL at birth as an assessment factor for radiation related hematopoietic challenges in children.","PeriodicalId":50452,"journal":{"name":"Fetal and Pediatric Pathology","volume":" ","pages":"315-329"},"PeriodicalIF":0.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141898767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Restriction of Medically Indicated Abortions. 限制有医学指征的堕胎。

IF 0.7 4区医学

Fetal and Pediatric Pathology Pub Date : 2024-05-01 Epub Date: 2023-12-26 DOI: 10.1080/15513815.2023.2298144

Randall Craver

引用次数: 0

Cell Free Microbial DNA Utilization at a Children's Hospital. 儿童医院的无细胞微生物 DNA 利用。

IF 1.1 4区医学

Fetal and Pediatric Pathology Pub Date : 2024-05-01 Epub Date: 2024-02-12 DOI: 10.1080/15513815.2024.2315434

Randall Craver, Stephanie Collier, Margot Anderson

引用次数: 0

MTHFR 677 C > T Gene Polymorphism is Associated with Large for Gestational Age Infants. MTHFR 677 C > T 基因多态性与巨大胎龄儿有关。