Journal of Infectious Diseases最新文献

{"title":"ICESp1109, a Novel Hybrid Integrative Conjugative Element of Macrolide-Resistant Streptococcus pyogenes Serotype M77 Collected Between 2003 and 2017 in Poland.","authors":"Jan Gawor, Karolina Żuchniewicz, Matthew Ojeda Saavedra, Stephen B Beres, Marlena Kiedrowska, Izabela Wróbel-Pawelczyk, Aleksandra Kozińska, Robert Gromadka, James M Musser, Izabela Sitkiewicz, Izabela Kern-Zdanowicz","doi":"10.1093/infdis/jiae473","DOIUrl":"10.1093/infdis/jiae473","url":null,"abstract":"<p><strong>Background: </strong>The antibiotic resistance determinants and associated mobile genetic elements (MGEs) were detected among Streptococcus pyogenes (group A streptococci [GAS]) clinical isolates of an M77 serotype collected in Poland between 2003 and 2017.</p><p><strong>Methods: </strong>The genomes of 136 M77 GAS isolates were sequenced using short- and selected with long-read approach; whole genome sequences were analyzed to determine the genetic context of macrolide resistance determinants.</p><p><strong>Results: </strong>The analysed strains were collected from in- and outpatients. Sequencing data analysis revealed that all strains carried the tet(O) gene. They were classified as a single sequence type, ST63. The unique erythromycin-resistance determinant, the erm(TR), was detected in 76.5% (n = 104) of isolates. It was found predominantly (n = 74) within a novel hybrid integrative conjugative element composed of the ICESp1108-like sequence and ICESp2906 variant, which was then named ICESp1109. However, in strains isolated before 2008, erm(TR) was located within ICESp2905 (n = 27) and in 3 strains - within stand-alone ICESp1108-like sequences.</p><p><strong>Conclusions: </strong>Based on phylogenetic analysis results, the clonal dissemination of the macrolide-resistant S. pyogenes M77/ST63 strain with hybrid ICESp1109 was observed between 2008 and 2017. ICESp1109 is the novel hybrid ICE in gram-positive bacteria.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":"e521-e530"},"PeriodicalIF":5.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142407145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Instrumental Variables Analysis and Mendelian Randomization for Causal Inference.","authors":"Erica E M Moodie, Saskia le Cessie","doi":"10.1093/infdis/jiae357","DOIUrl":"10.1093/infdis/jiae357","url":null,"abstract":"","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":"556-558"},"PeriodicalIF":5.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relating in vivo RSV infection kinetics to host infectiousness in different age groups.","authors":"Ke Li, Louis J Bont, Daniel M Weinberger, Virginia E Pitzer","doi":"10.1093/infdis/jiaf138","DOIUrl":"10.1093/infdis/jiaf138","url":null,"abstract":"<p><p>Respiratory syncytial virus (RSV) infections are a major public health concern for pediatric populations and older adults. Viral kinetics, the dynamic processes of viral infection within an individual over time, vary across different populations. However, RSV transmission in different age groups is incompletely understood from the perspective of individual-level viral kinetics. Using a mathematical model and a hierarchical Bayesian framework, we analyzed viral kinetics in 53 individuals from different age groups to estimate infection parameters and linked within-host viral load to transmission probability through a probabilistic model. We found that children had higher peak viral loads and longer shedding periods compared to other age groups, suggesting a higher transmission probability over the infectious period. We validated our findings by comparing the estimated secondary attack rate across different age groups to empirical estimates from household transmission studies. Our work highlights the importance of age-specific considerations in understanding and managing RSV infections.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Miniaturized assay to evaluate Plasmodium cynomolgi and P. knowlesi as models for prioritizing P. vivax vaccine targets.","authors":"Sheena Dass, Prasun Kundu, Deboki Naskar, Usheer Kanjee, Marcelo U Ferreira, Julian C Rayner, Manoj T Duraisingh","doi":"10.1093/infdis/jiaf136","DOIUrl":"https://doi.org/10.1093/infdis/jiaf136","url":null,"abstract":"<p><p>Plasmodium vivax vaccine discovery lags behind P. falciparum due to the absence of a reliable in vitro culture system. We developed a miniature 384-well assay for the evolutionarily related parasite species P. cynomolgi and P. knowlesi, deploying it for screening invasion inhibition efficacy of antibodies elicited against 8 P. vivax proteins. All 8 antibodies showed consistent inhibition across both P. knowlesi and P. cynomolgi species, correlating with inhibition in ex vivo P. vivax isolates. The use of simian malaria parasites and this assay represents a robust, high-throughput method for prioritizing P. vivax blood stage vaccine candidates.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical phenotypes and outcomes associated with respiratory syncytial virus infection in critically ill patients: a retrospective multicentre cohort study in Great Paris area hospitals, 2017-2023.","authors":"Antoine Gaillet, Richard Layese, Slim Fourati, Héloise Celante, Tài Pham, Sarah Benghanem, Armand Mekontso Dessap, Etienne de Montmollin, Jeoffrey Pirault, Antoine Vieillard-Baron, Eric Maury, Yves Cohen, Maxens Decavèle, Muriel Fartoukh, Nicholas Heming, Charles Damoisel, Nadia Oubaya, Nicolas de Prost","doi":"10.1093/infdis/jiaf129","DOIUrl":"https://doi.org/10.1093/infdis/jiaf129","url":null,"abstract":"<p><strong>Background: </strong>Respiratory syncytial virus (RSV) is one of the deadliest respiratory viruses. There is a need to better identify prognostic factors in RSV-infected patients, particularly those requiring intensive care unit (ICU) admission, with a focus on immunosuppressed patients.</p><p><strong>Methods: </strong>Multicenter, retrospective cohort study of RSV-infected adults hospitalized in 17 ICUs in the Great Paris area between 08/01/2017 and 05/01/2023. The primary endpoint was all cause day 30 mortality. Supervised and unsupervised analyses were performed.</p><p><strong>Results: </strong>During the study period, 474 patients with RSV infection were admitted. Patients were predominantly male (56%), were 65±17-years old, had frequent cardiovascular and respiratory comorbidities and 34% were immunosuppressed. Day 30 mortality was 14%. Besides age >65 years and acute respiratory failure, immunosuppression was associated with day 30 mortality in multivariable logistic regression analysis (adjusted odds ratio=2.10 95% confidence interval [1.14;3.80]; p=0.035). Unsupervised cluster analysis identified three groups of patients: one cluster (17%) included only immunosuppressed patients and had the highest day 30 mortality rate (21%); the second cluster (43%) included older patients (median age 71 years) with a high prevalence of cardiovascular and respiratory comorbidities and an intermediate mortality rate (14%); the last cluster (37%) included younger patients (median age 57 years) with the lowest mortality rate (9%).</p><p><strong>Conclusion: </strong>One third of patients admitted to the ICU with RSV infection were immunosuppressed. Both supervised and unsupervised methods identified immunosuppression as being associated with day 30 mortality. The effect of anti-RSV therapies aimed at preventing ICU admission should be evaluated in this subgroup of patients.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV-2 infection of human lung ALI cultures reveals basal cells as relevant targets.","authors":"Morris Baumgardt, Benedikt Obermayer, Anita Balázs, Anna Löwa, Emanuel Wyler, Luiz Gustavo Teixeira Alves, Katharina Hellwig, Dieter Beule, Markus Landthaler, Marcel A Müller, Christian Drosten, Marcus A Mall, Stefan Hippenstiel, Katja Hönzke, Andreas C Hocke","doi":"10.1093/infdis/jiaf125","DOIUrl":"https://doi.org/10.1093/infdis/jiaf125","url":null,"abstract":"<p><p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) primarily targets ciliated cells during the initial infection of the upper respiratory tract. Since uncertainties persist regarding other involved epithelial cell types, we here utilized viral replication analysis, single-cell RNA sequencing, and spectral microscopy on infected air-liquid interface cultures of human primary nasal and bronchial epithelial cells to discern cell type proportions in relation to SARS-CoV-2 tropism and immune activation. We revealed that, next to ciliated and secretory cells, SARS-CoV-2 (wild type and lineage B1.1.7 [Alpha variant]) strongly infects basal cells, significantly contributing to the epithelial immune response in a donor-specific manner. Moreover, local Camostat mesylate treatment was effective on both the basal and apical cell compartment, resulting in a notable reduction in viral load and reduced immune activation. Collectively, our data emphasize the critical role of basal cells in facilitating SARS-CoV-2 dissemination within the upper respiratory tract and their substantial contribution to the epithelial immune response. Furthermore, our results highlight the potential of local application of Camostat mesylate as an effective strategy for inhibiting SARS-CoV-2 infection and mitigating associated immune activation early on.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143598236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"10 Years of Severe Acute Respiratory Infections Network (SARInet plus): Accomplishments and Way Forward.","authors":"Marc Rondy, Eduardo Azziz-Baumgartner, Rebecca Kondor, Rakhee S Palekar, Wenqing Zhang, Andrea S Vicari","doi":"10.1093/infdis/jiaf039","DOIUrl":"10.1093/infdis/jiaf039","url":null,"abstract":"","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":"S87-S89"},"PeriodicalIF":5.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pandemic Preparedness: Analyzing National Plans for Respiratory Pathogen Pandemics in the Americas Region.","authors":"Andrea Patricia Villalobos Rodríguez, Iyanna Wellington Perkins, Shoa Moosavi, Ana de la Garza, Ángel Rodríguez, Britney McMurren, Juliana Almeida Leite, Jairo Méndez-Rico, Lidia Redondo-Bravo, Miriam Esther Blanco Reyes, Olaya Astudillo, Paula Couto, Priscila S Born, Sarah Hess, Shoshanna Goldin, Hannah Lewis, Tamara Mancero, Andrea S Vicari, Marc Rondy","doi":"10.1093/infdis/jiaf047","DOIUrl":"10.1093/infdis/jiaf047","url":null,"abstract":"<p><strong>Background: </strong>To identify gaps and define priority actions to strengthen national pandemic preparedness and response plans, we assessed the concordance between national plans for respiratory pathogens against the World Health Organization (WHO) pandemic checklist and the States Parties Annual Report (SPAR) in the Americas.</p><p><strong>Methods: </strong>In this retrospective, semiquantitative study, we reviewed the most recent respiratory pandemic plans for 35 Pan American Health Organization (PAHO) member states and assessed their concordance with (1) actionable guidelines in WHO pandemic checklist and (2) International Health Regulations (IHR) (2005) core capacities using the latest SPAR tool. We developed 25 tracking questions to identify gaps, strengths, and opportunities for improvement using a 5-point scale. We compared the average SPAR score and the Pandemic Plans score to assess areas of convergence and variance between preparedness and capacities.</p><p><strong>Results: </strong>We analyzed 35 respiratory pandemic plans (2005-2024): 29 were influenza specific, 5 were COVID-19 specific, and 1 was not pathogen specific. Most national plans showed limited alignment with the content recommended in the pandemic checklist. The lowest concordance between plans and checklist was in public health and social measures (80% of plans with score of 1); emergency, logistics and supply chain management (74%); and research and development (71%). Conversely, the strongest subcomponents were policy, legal, and normative instruments (45% of plans with score 4 or 5); coordination (46%); and surveillance: early detection and assessment (43%).</p><p><strong>Conclusions: </strong>It is recommended that countries build on the strengths of their national pandemic preparedness and response plans and update them using PRET module 1. This will support countries advance the capacities required by the IHR.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":"S90-S99"},"PeriodicalIF":5.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strengthening the Surveillance and Response to Public Health Events With a One Health Approach: A Perspective From 12 Countries in Latin America and the Caribbean.","authors":"Ángel Rodríguez, Paula Couto, Alejandra Acevedo, Belsy Acosta Herrera, Olaya Astudillo, Martín Avaro, Gisela Barrera Badillo, Alfredo Bruno, Patricia Bustos, Mauricio Cerpa, Hector Chiparelli, Dulce Duron, Rodrigo Fasce, Axel Galo, Elías Guilarte, Irma López Martínez, Mariela G Martínez, Jairo Méndez-Rico, María Fernanda Olivares Barraza, Elena Penayo, María Paz Rojas Mena, Paula Rodríguez Ferrari, Marc Rondy, Viviana Sotomayor Proschle, Priscila S Born, Lidia Redondo-Bravo, Juliana Almeida Leite, Natalia Vergara Mallegas, Marta von Horoch, Andrea Patricia Villalobos Rodríguez, Andrea S Vicari","doi":"10.1093/infdis/jiae629","DOIUrl":"10.1093/infdis/jiae629","url":null,"abstract":"<p><p>The Surveillance Network for Influenza and Other Respiratory Viruses (SARInet) has played a crucial role in improving the capacity of Latin American countries to monitor and respond to events with potential public health impact. This perspective compiles and analyzes case studies from several countries in the Americas to illustrate the challenges faced prior to the creation of SARInet, the interventions implemented, and the impacts achieved.</p><p><strong>Methodology: </strong>This study is based on a series of cases from 12 countries in Latin America and the Caribbean. The data have been compiled from reports, ad-hoc assessments and technical group analyses conducted before and after the implementation of SARInet in 2014.</p><p><strong>Results: </strong>Over the past decade, SARInet has been instrumental in strengthening the capacity for early detection and response to public health events in several Latin American countries. Technical cooperation in epidemiological and laboratory surveillance, personnel training, intersectoral coordination and the adoption of advanced technologies have improved the countries' capacity for timely detection and management of epidemic and pandemic outbreaks.</p><p><strong>Conclusions: </strong>The experience of the countries shows that regional collaboration and the strengthening of local capacities are essential to face future public health challenges.</p>","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":"S108-S113"},"PeriodicalIF":5.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Epidemics to Pandemics: Over a Decade of Strengthening SARInet Laboratory Surveillance and Response for Respiratory Viruses in the Americas.","authors":"Juliana Almeida Leite, Priscila S Born, Ángel Rodríguez, Paula Couto, Mauricio Cerpa, Jean-Marc Gabastou, Lionel Gresh, Leticia Franco, Mariela G Martínez, Madelaine Sugasti, Jorge H Jara, Lidia Rodondo-Bravo, Iyanna Wellington Perkins, Andrea Patricia Villalobos Rodríguez, Rosa Ramirez, Ximena Terrazas, Juliana Barbosa, Marc Rondy, Andrea S Vicari, Sylvain Aldighieri, Richard Webby, Wenqing Zhang, Dimitry Pereyaslov, Magdi Samaan, Jairo Méndez-Rico","doi":"10.1093/infdis/jiaf046","DOIUrl":"10.1093/infdis/jiaf046","url":null,"abstract":"","PeriodicalId":50179,"journal":{"name":"Journal of Infectious Diseases","volume":" ","pages":"S100-S107"},"PeriodicalIF":5.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11892004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}